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The map label for this gene is thiM2

Identifier: 23098098

GI number: 23098098

Start: 677948

End: 678730

Strand: Reverse

Name: thiM2

Synonym: OB0643

Alternate gene names: 23098098

Gene position: 678730-677948 (Counterclockwise)

Preceding gene: 23098105

Following gene: 23098097

Centisome position: 18.7

GC content: 39.97

Gene sequence:

>783_bases
TTGGTGGATGCTATTGCTAAGATTAAACAAGATAAACCGTTTATTTTTAATATAACCAATGAAGTTGCATCTAATTTTTC
TGCTAATGGCTTAATCGCTATCGGAGCGTCACCTGCGATGTCTCATACTCCAAGAGAAGCTAAAAAGTATGGAGAAATCG
CCGACGCGGTAGTGCTAAACCTGGGGACATTAACGGAAGACCGGGGAGAAGCAATGTTAAAGGCTGGAATGGCAGCAAAC
AAGAATGGGGTTCCAGTTATTCTTGATCCCATTGCAGTTGGAGGTACAGATTTTCGGACGGAGATCATTGATGACATACT
TAAAACGGTAAAGCTAGCTGCAATACGTGCTAATGCTGGGGAAATAGCTGTGTTGGCAGGTAAGCTAGAAAAAGCAAAGG
GGCCAGATTCCATTATTCAAGAAAACGAACCAGAAATCGCAAAAACTGTCGCTAAAAAATATGATACGGTTGTCATCTCA
ACTGGAAAAGTTGACGTTGTGACAGATGGTAAGCGAACTGCTTTATGTACAAACGGGCATGAAATGCTACAAAACATTAC
GGCTAGCGGATGTTTACTTTCATCTTTTGTCGGACCTTTTGTGAGTGTAGTTGATGACTTTTATCAAGCAGGTATATATG
CTGTGACAAGTTATGGTATTGCAGCTGAATTAGCAATGGAAAAAGCATCTGGGCCTGGAACATTTATTCCGGCATTACTC
GATGAAATATACTTTCTAACTGATGAAAAAGTAGAAAAATACAAACAAGTTCACGAACTATAA

Upstream 100 bases:

>100_bases
GAGTTGGCGACTATACCTATTCTGTAGCTGTATATAAAACCACTCCATTTTGGGGTGGTTTATTTGTTCTTTATTCTAAA
TTAATAGGAGAGGAGAATTT

Downstream 100 bases:

>100_bases
GGATATGTATCATTATCGAAGGAGGAAAAGTAATTGGAAAATCTTCTATTTGTCGAGACAGGAACAGGAGTAGATGTTCA
TGGACAGAACGTGAATATTG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 2; TH kinase 2; Thz kinase 2

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MVDAIAKIKQDKPFIFNITNEVASNFSANGLIAIGASPAMSHTPREAKKYGEIADAVVLNLGTLTEDRGEAMLKAGMAAN
KNGVPVILDPIAVGGTDFRTEIIDDILKTVKLAAIRANAGEIAVLAGKLEKAKGPDSIIQENEPEIAKTVAKKYDTVVIS
TGKVDVVTDGKRTALCTNGHEMLQNITASGCLLSSFVGPFVSVVDDFYQAGIYAVTSYGIAAELAMEKASGPGTFIPALL
DEIYFLTDEKVEKYKQVHEL

Sequences:

>Translated_260_residues
MVDAIAKIKQDKPFIFNITNEVASNFSANGLIAIGASPAMSHTPREAKKYGEIADAVVLNLGTLTEDRGEAMLKAGMAAN
KNGVPVILDPIAVGGTDFRTEIIDDILKTVKLAAIRANAGEIAVLAGKLEKAKGPDSIIQENEPEIAKTVAKKYDTVVIS
TGKVDVVTDGKRTALCTNGHEMLQNITASGCLLSSFVGPFVSVVDDFYQAGIYAVTSYGIAAELAMEKASGPGTFIPALL
DEIYFLTDEKVEKYKQVHEL
>Mature_260_residues
MVDAIAKIKQDKPFIFNITNEVASNFSANGLIAIGASPAMSHTPREAKKYGEIADAVVLNLGTLTEDRGEAMLKAGMAAN
KNGVPVILDPIAVGGTDFRTEIIDDILKTVKLAAIRANAGEIAVLAGKLEKAKGPDSIIQENEPEIAKTVAKKYDTVVIS
TGKVDVVTDGKRTALCTNGHEMLQNITASGCLLSSFVGPFVSVVDDFYQAGIYAVTSYGIAAELAMEKASGPGTFIPALL
DEIYFLTDEKVEKYKQVHEL

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=247, Percent_Identity=36.4372469635627, Blast_Score=157, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=284, Percent_Identity=31.3380281690141, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM2_OCEIH (Q8ESJ1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691564.1
- ProteinModelPortal:   Q8ESJ1
- SMR:   Q8ESJ1
- GeneID:   1016041
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0643
- NMPDR:   fig|221109.1.peg.649
- HOGENOM:   HBG351126
- ProtClustDB:   PRK09355
- BioCyc:   OIHE221109:OB0643-MONOMER
- BRENDA:   2.7.1.50
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27636; Mature: 27636

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: BINDING 40-40 BINDING 116-116 BINDING 161-161 BINDING 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDAIAKIKQDKPFIFNITNEVASNFSANGLIAIGASPAMSHTPREAKKYGEIADAVVLN
CCCHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
LGTLTEDRGEAMLKAGMAANKNGVPVILDPIAVGGTDFRTEIIDDILKTVKLAAIRANAG
HCCCCCHHHHHHHHHCCCCCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
EIAVLAGKLEKAKGPDSIIQENEPEIAKTVAKKYDTVVISTGKVDVVTDGKRTALCTNGH
CEEEEECCHHHCCCCHHHHCCCCCHHHHHHHHHHCEEEEECCEEEEEECCCCEEEECCHH
EMLQNITASGCLLSSFVGPFVSVVDDFYQAGIYAVTSYGIAAELAMEKASGPGTFIPALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCHHHHH
DEIYFLTDEKVEKYKQVHEL
HHHHHHCHHHHHHHHHHHCC
>Mature Secondary Structure
MVDAIAKIKQDKPFIFNITNEVASNFSANGLIAIGASPAMSHTPREAKKYGEIADAVVLN
CCCHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
LGTLTEDRGEAMLKAGMAANKNGVPVILDPIAVGGTDFRTEIIDDILKTVKLAAIRANAG
HCCCCCHHHHHHHHHCCCCCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
EIAVLAGKLEKAKGPDSIIQENEPEIAKTVAKKYDTVVISTGKVDVVTDGKRTALCTNGH
CEEEEECCHHHCCCCHHHHCCCCCHHHHHHHHHHCEEEEECCEEEEEECCCCEEEECCHH
EMLQNITASGCLLSSFVGPFVSVVDDFYQAGIYAVTSYGIAAELAMEKASGPGTFIPALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCHHHHH
DEIYFLTDEKVEKYKQVHEL
HHHHHHCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376