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The map label for this gene is yfkN [H]

Identifier: 23098053

GI number: 23098053

Start: 625525

End: 627105

Strand: Reverse

Name: yfkN [H]

Synonym: OB0598

Alternate gene names: 23098053

Gene position: 627105-625525 (Counterclockwise)

Preceding gene: 23098054

Following gene: 23098045

Centisome position: 17.27

GC content: 34.66

Gene sequence:

>1581_bases
TTGCACCAATCAGCTGTAACTATTTTATACACAAGTGATGTACACGGACACGCAATGCCAATACAGTATGGGACAAACAA
AAAAGAAGATATTGGACTTGCTAAATATGCAACCGTGGTAGAAGAACATCGAGAATCCGAAGAGAATTTAATTATTATTG
ATAATGGTGATTTAATACAAGGCACACCACTTATGACACATTATGTGAAGGAACATTTAGATAAGCAAAATCCTATGGTA
GGAATAATGAATCAATTACACATAGACGCAGGAGTAATCGGTAACCATGAATTTAACTTTGGAAAATCCGTTTTACAACA
AGCCATCCATGAATCTAATTTCCCTTGGTTATCTGCTAACATAATAAACAACCAAACAAAAGAACCTGCATTCGGTCCGC
CTTATATTATAAAAACATTTCATAATGGAATAACTGCAGCAATTGTAGGGGTGACTACCCATTACATTCCTAATTGGGAG
ACTCCTGAGCATATTGAAGGTTTATTATTTGAAGATGCATTTGATACGTTGAATAGTTGGGTTTCTTACATTAGAGAGAA
CGAGCAGCCTGATATTGTAATTGCTGCTTATCATGGAGGATTTGAAAAAGACTTACATACAGGAGAAGCTACTGAAGATT
TAACTGGTGAAAACCAAGGCTATCAAATAGTTGAAGAGATTGAGGGCATCGATATTTTGCTCACTGGTCATCAACATCGA
CGCATTAATGAGGTTAATAATAATATCATCGTTTTACAACCAGGAAACAATGGACAAGCATATGGAAAAGTCGAAATATC
CTTGACCAAAGATAATAATAGTAGTTGGATAATCGAGAATAAGAAAGGTGCTATTCATGAACTTACCGGTGTAAAATCCA
ACCAGCAAATACTTAACTACATGGAAGCATTAGAAGCTTCTACGCAGACATGGTTAGATCAACCTATCGGGAAGATTGAT
GGCGATATGATTATTCATGACCCCTTTCAAGCACGTATTAAAAAGCATCCTTTTATAGAATTTATACATAACGTACAAAT
GGATGCAGCAGAAGTTGATATATCCGTATCTGCGTTATTAAACAATACGTCAAAAGGATTTTCCTCAGAAGTTACGATGC
GAGATATTGTATCTAATTATATGTATCCCAATACGTTAACTGTATTAGAATTAACGGGGAAAGACATAAAAGAAGCTTTG
GAAAAAAGCGCAGAATACTTTACAATTAAAAATAATCAAATTACCGTCAATCCTAATTATATCGAACCAAAACCACAACA
TTACAATTATGATATCTGGCATGGAATTGACTATACGATGAATATATCAAATTCACCAGGTGATCGTGTTTCCAATATGC
GATATCACGGTGAACCAATAAAATCCGATGATACATTTCACGTTGTATTAAATAATTACCGTGCATCTGGCGGTGGTAAT
TACGATATGTTTAAACATAAACCAGTAATAAAAGAAATACAAAGAGATGCAGTAGAACTTATACAAACATATTTTGAAAA
ACACCCCCGTGTTGCTGCAACTACGACAAATAATTATCAGGTGGTTATGGATCACAATTAA

Upstream 100 bases:

>100_bases
AGAAACCTATTAATAGTTTTCGTCCTCGAGTAAACTCCTTTATGCTCGAGGACTTTTTAACGATTATCGTCAAGTAGTAC
TATAGAAAGGATGTTTTTGA

Downstream 100 bases:

>100_bases
CGAGGAACATCAATTAGAATTGAAATTGGAATTGGACAGCTTGGTTCATGATTCTTAGGTACTGAATGACGAGCCAACCC
TGGCAATTTAGTAAGTCCGT

Product: phosphatase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MHQSAVTILYTSDVHGHAMPIQYGTNKKEDIGLAKYATVVEEHRESEENLIIIDNGDLIQGTPLMTHYVKEHLDKQNPMV
GIMNQLHIDAGVIGNHEFNFGKSVLQQAIHESNFPWLSANIINNQTKEPAFGPPYIIKTFHNGITAAIVGVTTHYIPNWE
TPEHIEGLLFEDAFDTLNSWVSYIRENEQPDIVIAAYHGGFEKDLHTGEATEDLTGENQGYQIVEEIEGIDILLTGHQHR
RINEVNNNIIVLQPGNNGQAYGKVEISLTKDNNSSWIIENKKGAIHELTGVKSNQQILNYMEALEASTQTWLDQPIGKID
GDMIIHDPFQARIKKHPFIEFIHNVQMDAAEVDISVSALLNNTSKGFSSEVTMRDIVSNYMYPNTLTVLELTGKDIKEAL
EKSAEYFTIKNNQITVNPNYIEPKPQHYNYDIWHGIDYTMNISNSPGDRVSNMRYHGEPIKSDDTFHVVLNNYRASGGGN
YDMFKHKPVIKEIQRDAVELIQTYFEKHPRVAATTTNNYQVVMDHN

Sequences:

>Translated_526_residues
MHQSAVTILYTSDVHGHAMPIQYGTNKKEDIGLAKYATVVEEHRESEENLIIIDNGDLIQGTPLMTHYVKEHLDKQNPMV
GIMNQLHIDAGVIGNHEFNFGKSVLQQAIHESNFPWLSANIINNQTKEPAFGPPYIIKTFHNGITAAIVGVTTHYIPNWE
TPEHIEGLLFEDAFDTLNSWVSYIRENEQPDIVIAAYHGGFEKDLHTGEATEDLTGENQGYQIVEEIEGIDILLTGHQHR
RINEVNNNIIVLQPGNNGQAYGKVEISLTKDNNSSWIIENKKGAIHELTGVKSNQQILNYMEALEASTQTWLDQPIGKID
GDMIIHDPFQARIKKHPFIEFIHNVQMDAAEVDISVSALLNNTSKGFSSEVTMRDIVSNYMYPNTLTVLELTGKDIKEAL
EKSAEYFTIKNNQITVNPNYIEPKPQHYNYDIWHGIDYTMNISNSPGDRVSNMRYHGEPIKSDDTFHVVLNNYRASGGGN
YDMFKHKPVIKEIQRDAVELIQTYFEKHPRVAATTTNNYQVVMDHN
>Mature_526_residues
MHQSAVTILYTSDVHGHAMPIQYGTNKKEDIGLAKYATVVEEHRESEENLIIIDNGDLIQGTPLMTHYVKEHLDKQNPMV
GIMNQLHIDAGVIGNHEFNFGKSVLQQAIHESNFPWLSANIINNQTKEPAFGPPYIIKTFHNGITAAIVGVTTHYIPNWE
TPEHIEGLLFEDAFDTLNSWVSYIRENEQPDIVIAAYHGGFEKDLHTGEATEDLTGENQGYQIVEEIEGIDILLTGHQHR
RINEVNNNIIVLQPGNNGQAYGKVEISLTKDNNSSWIIENKKGAIHELTGVKSNQQILNYMEALEASTQTWLDQPIGKID
GDMIIHDPFQARIKKHPFIEFIHNVQMDAAEVDISVSALLNNTSKGFSSEVTMRDIVSNYMYPNTLTVLELTGKDIKEAL
EKSAEYFTIKNNQITVNPNYIEPKPQHYNYDIWHGIDYTMNISNSPGDRVSNMRYHGEPIKSDDTFHVVLNNYRASGGGN
YDMFKHKPVIKEIQRDAVELIQTYFEKHPRVAATTTNNYQVVMDHN

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=211, Percent_Identity=31.2796208530806, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790658, Length=544, Percent_Identity=26.4705882352941, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1786687, Length=535, Percent_Identity=22.0560747663551, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921980, Length=500, Percent_Identity=23.4, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161076508, Length=500, Percent_Identity=23.4, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652512, Length=500, Percent_Identity=23.4, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 59589; Mature: 59589

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQSAVTILYTSDVHGHAMPIQYGTNKKEDIGLAKYATVVEEHRESEENLIIIDNGDLIQ
CCCCEEEEEEECCCCCCEEEEEECCCCCHHCCHHHHHHHHHHHHCCCCCEEEEECCCEEE
GTPLMTHYVKEHLDKQNPMVGIMNQLHIDAGVIGNHEFNFGKSVLQQAIHESNFPWLSAN
CCHHHHHHHHHHHCCCCCHHHHHHHHEECCEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
IINNQTKEPAFGPPYIIKTFHNGITAAIVGVTTHYIPNWETPEHIEGLLFEDAFDTLNSW
ECCCCCCCCCCCCCHHEEECCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
VSYIRENEQPDIVIAAYHGGFEKDLHTGEATEDLTGENQGYQIVEEIEGIDILLTGHQHR
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCCCEEEEECCCCH
RINEVNNNIIVLQPGNNGQAYGKVEISLTKDNNSSWIIENKKGAIHELTGVKSNQQILNY
HHHHCCCCEEEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCEEHHHCCCCHHHHHHH
MEALEASTQTWLDQPIGKIDGDMIIHDPFQARIKKHPFIEFIHNVQMDAAEVDISVSALL
HHHHHHHHHHHHHCCHHHCCCCEEEECCHHHHHCCCCHHHHHHHCCCCHHHCCEEEEHHH
NNTSKGFSSEVTMRDIVSNYMYPNTLTVLELTGKDIKEALEKSAEYFTIKNNQITVNPNY
CCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCEEEEECCEEEECCCC
IEPKPQHYNYDIWHGIDYTMNISNSPGDRVSNMRYHGEPIKSDDTFHVVLNNYRASGGGN
CCCCCCCCCEEEEECCEEEEECCCCCCCHHCCCEECCCCCCCCCEEEEEEECEECCCCCC
YDMFKHKPVIKEIQRDAVELIQTYFEKHPRVAATTTNNYQVVMDHN
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECC
>Mature Secondary Structure
MHQSAVTILYTSDVHGHAMPIQYGTNKKEDIGLAKYATVVEEHRESEENLIIIDNGDLIQ
CCCCEEEEEEECCCCCCEEEEEECCCCCHHCCHHHHHHHHHHHHCCCCCEEEEECCCEEE
GTPLMTHYVKEHLDKQNPMVGIMNQLHIDAGVIGNHEFNFGKSVLQQAIHESNFPWLSAN
CCHHHHHHHHHHHCCCCCHHHHHHHHEECCEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
IINNQTKEPAFGPPYIIKTFHNGITAAIVGVTTHYIPNWETPEHIEGLLFEDAFDTLNSW
ECCCCCCCCCCCCCHHEEECCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
VSYIRENEQPDIVIAAYHGGFEKDLHTGEATEDLTGENQGYQIVEEIEGIDILLTGHQHR
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHCCCCCCCCHHHHHCCCCEEEEECCCCH
RINEVNNNIIVLQPGNNGQAYGKVEISLTKDNNSSWIIENKKGAIHELTGVKSNQQILNY
HHHHCCCCEEEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCEEHHHCCCCHHHHHHH
MEALEASTQTWLDQPIGKIDGDMIIHDPFQARIKKHPFIEFIHNVQMDAAEVDISVSALL
HHHHHHHHHHHHHCCHHHCCCCEEEECCHHHHHCCCCHHHHHHHCCCCHHHCCEEEEHHH
NNTSKGFSSEVTMRDIVSNYMYPNTLTVLELTGKDIKEALEKSAEYFTIKNNQITVNPNY
CCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCEEEEECCEEEECCCC
IEPKPQHYNYDIWHGIDYTMNISNSPGDRVSNMRYHGEPIKSDDTFHVVLNNYRASGGGN
CCCCCCCCCEEEEECCEEEEECCCCCCCHHCCCEECCCCCCCCCEEEEEEECEECCCCCC
YDMFKHKPVIKEIQRDAVELIQTYFEKHPRVAATTTNNYQVVMDHN
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]