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The map label for this gene is thrB

Identifier: 23097919

GI number: 23097919

Start: 492111

End: 492995

Strand: Reverse

Name: thrB

Synonym: OB0464

Alternate gene names: 23097919

Gene position: 492995-492111 (Counterclockwise)

Preceding gene: 23097920

Following gene: 23097916

Centisome position: 13.58

GC content: 38.31

Gene sequence:

>885_bases
ATGACGCCTTTTGGTATCTCTGTTCCAGCAAGCAGCGGTAATGTGGGCCCTGGTTTTGATTCAACAGGGCTTGCATTAGG
CTTATACTTACATCTAAATGTAGAGGATGCGAACAGCATACAATTTTATTCCGACGGCGAATCTACCCCTACAGAAAACC
ACTTTATATGGAATATTGCAGAAAACATTGCTAATAAACACGGTATTCGTCTACCTGCTTGCAAGGTACAGGAAACTAAC
GAGATTCCTTTGGCTCGTGGTTTAGGAAGCAGCGCTTCTGCAATTGTTGCAGGTATCGAGCTTGCCAATCAATTAGGAAA
TCTACATTTAACACCAGAACAAAAATTACAATACGGCACGGAAATTGAAGGGCATCCTGATAATGTTGCACCTTCTATCT
ATGGTGGATTAGTTATTTCAACAGTATTAGAGAAAGAAATTGAACATATCCAATTGAGAGACATTGATGTCGATATAGTT
GCCTATATACCAAATATAGAACTAAAGACATCCGTATCTAGAAATTGCTTACCTGATAGCTACAATCGAGATTATGCTGC
TAAAGCAAGTGCAATTAGCAATTTAACTATTGCTGCATTATACAGTAAGGATTACAAATTAGCAGGTAAACTAATGGAAG
AAGATCTCTTTCATGAACCTTTTCGTTCAGAGCTAATTCCAAATTTTGCTTATATTCGAGAGGAAGCGAAAAAGTATGGC
GCATTTGGCACTATCTTAAGTGGCGCAGGTCCGACTATGCTATCCATAACTCCAAAAGGAAACGGACCAACTTTACAAAA
CAATATGTCTAAGTTGTCTTTACTTGCGGATTACCAAGTTGAATACATACCTATCGACTATCAAGGTATCTCCATTCAAG
AATAA

Upstream 100 bases:

>100_bases
AAGATCCACAAACGGCAATCGATCAACTATCCATTGATCCTGTAAGCTTACCAAACAACGAAAAAGCAGTCATGGACTAT
CTTGAAGAGTTGATTACATC

Downstream 100 bases:

>100_bases
GTTAGCAATTATGTAATTGACTTATTTGTATGCATTCGAAAATAGAGGCTGAGACAAAAGTATTTAATCAAAAGACAAAT
CCAAATGATTGGACTATAAA

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTPFGISVPASSGNVGPGFDSTGLALGLYLHLNVEDANSIQFYSDGESTPTENHFIWNIAENIANKHGIRLPACKVQETN
EIPLARGLGSSASAIVAGIELANQLGNLHLTPEQKLQYGTEIEGHPDNVAPSIYGGLVISTVLEKEIEHIQLRDIDVDIV
AYIPNIELKTSVSRNCLPDSYNRDYAAKASAISNLTIAALYSKDYKLAGKLMEEDLFHEPFRSELIPNFAYIREEAKKYG
AFGTILSGAGPTMLSITPKGNGPTLQNNMSKLSLLADYQVEYIPIDYQGISIQE

Sequences:

>Translated_294_residues
MTPFGISVPASSGNVGPGFDSTGLALGLYLHLNVEDANSIQFYSDGESTPTENHFIWNIAENIANKHGIRLPACKVQETN
EIPLARGLGSSASAIVAGIELANQLGNLHLTPEQKLQYGTEIEGHPDNVAPSIYGGLVISTVLEKEIEHIQLRDIDVDIV
AYIPNIELKTSVSRNCLPDSYNRDYAAKASAISNLTIAALYSKDYKLAGKLMEEDLFHEPFRSELIPNFAYIREEAKKYG
AFGTILSGAGPTMLSITPKGNGPTLQNNMSKLSLLADYQVEYIPIDYQGISIQE
>Mature_293_residues
TPFGISVPASSGNVGPGFDSTGLALGLYLHLNVEDANSIQFYSDGESTPTENHFIWNIAENIANKHGIRLPACKVQETNE
IPLARGLGSSASAIVAGIELANQLGNLHLTPEQKLQYGTEIEGHPDNVAPSIYGGLVISTVLEKEIEHIQLRDIDVDIVA
YIPNIELKTSVSRNCLPDSYNRDYAAKASAISNLTIAALYSKDYKLAGKLMEEDLFHEPFRSELIPNFAYIREEAKKYGA
FGTILSGAGPTMLSITPKGNGPTLQNNMSKLSLLADYQVEYIPIDYQGISIQE

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=275, Percent_Identity=32.3636363636364, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321814, Length=314, Percent_Identity=30.5732484076433, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_OCEIH (Q8ET01)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691385.1
- ProteinModelPortal:   Q8ET01
- GeneID:   1015733
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0464
- NMPDR:   fig|221109.1.peg.470
- HOGENOM:   HBG646290
- OMA:   GLYDEVD
- ProtClustDB:   PRK01212
- BioCyc:   OIHE221109:OB0464-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 31958; Mature: 31827

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFGISVPASSGNVGPGFDSTGLALGLYLHLNVEDANSIQFYSDGESTPTENHFIWNIA
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEEEH
ENIANKHGIRLPACKVQETNEIPLARGLGSSASAIVAGIELANQLGNLHLTPEQKLQYGT
HHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCEEECHHHHHCCCC
EIEGHPDNVAPSIYGGLVISTVLEKEIEHIQLRDIDVDIVAYIPNIELKTSVSRNCLPDS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEECCCEEECCCCCCCCCCC
YNRDYAAKASAISNLTIAALYSKDYKLAGKLMEEDLFHEPFRSELIPNFAYIREEAKKYG
CCCCHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHC
AFGTILSGAGPTMLSITPKGNGPTLQNNMSKLSLLADYQVEYIPIDYQGISIQE
CHHEEECCCCCEEEEEEECCCCCCCHHCHHHEEEEECCEEEEEEECCCCCEECC
>Mature Secondary Structure 
TPFGISVPASSGNVGPGFDSTGLALGLYLHLNVEDANSIQFYSDGESTPTENHFIWNIA
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEEEH
ENIANKHGIRLPACKVQETNEIPLARGLGSSASAIVAGIELANQLGNLHLTPEQKLQYGT
HHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCEEECHHHHHCCCC
EIEGHPDNVAPSIYGGLVISTVLEKEIEHIQLRDIDVDIVAYIPNIELKTSVSRNCLPDS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEECCCEEECCCCCCCCCCC
YNRDYAAKASAISNLTIAALYSKDYKLAGKLMEEDLFHEPFRSELIPNFAYIREEAKKYG
CCCCHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHC
AFGTILSGAGPTMLSITPKGNGPTLQNNMSKLSLLADYQVEYIPIDYQGISIQE
CHHEEECCCCCEEEEEEECCCCCCCHHCHHHEEEEECCEEEEEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376