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The map label for this gene is opuD [H]

Identifier: 23097855

GI number: 23097855

Start: 424580

End: 426097

Strand: Reverse

Name: opuD [H]

Synonym: OB0400

Alternate gene names: 23097855

Gene position: 426097-424580 (Counterclockwise)

Preceding gene: 23097857

Following gene: 23097854

Centisome position: 11.74

GC content: 36.96

Gene sequence:

>1518_bases
ATGAAAAAGGTCACGAATGTTTTTTATTTCGCAGTTATTTTAGTTGGTATTATGGTTGCACTTGGTGCCATTTTCCCAGT
GAAATTTGAACAAATTTCACAAAGCGTTCAAGCTTGGGTATCATCTACTTTTGGTTGGTATTATTTATTACTAATGTCTG
TTTTAGTTGCTGTAGCACTATTTATTGCTGTTAGTCCTTATGGTAATATTCGCCTTGGGAAAGATAGCGATCGTCCTGAT
TTTTCAAATATTTCTTGGGTAGCTATGTTGTTCGCTGCGGGAATTGGTATTGGTTTAGTCTTTTACGGGGCATCGGAGCC
ACTTGCTCACTTTATGATTGATGCGCCTCTATCAGAACCAGGGACTGCACAAGCATTTAAAGAAGCTTTATCGCATTCAT
TCTTTCATTGGGGAATACATGGATGGTCCATGTATGGACTCGTCGCTCTTGCTATTGCATACTTTCAATTCAGAAAAGAT
GAACCTGGACTTATCTCCGTTACATTAAAGCCTTTATTTGGCGATCGAATGAATGGGGGATTCGGTAAAGCAATTGATGC
TTTAGCGATCTTCGCAACAGTAGTTGGAGTGGCTACATCTCTTGGTTTAGGTGCAATCCAGATTAATGCTGGCTTATCCC
ATCTATTTGATCTTCCAGTTAATATCGGCGTACAGGCAACTATCATATTAATTGTTACTGTATTATTTATAATATCAGCT
TGGAGTGGGTTAAATAAAGGAATTAAGACTCTATCCAACATTAATCTTATACTTGCTTTAATACTTTTTATCTTTGTATT
AGCCGTCGGTCCTACATTATTAATATTTAACATGTTCTCCGAATCAATTGGCTTGTATTTTCAAAATTTTATTCAGATGA
GCTTCCGATCCGCTCCTCTAGCATCAGATAATCGAGCATGGTTGGATATATGGACAATATTCTACTGGGCATGGTGGATA
TCTTGGGCACCATTCGTAGGTATCTTCGTTGCACGTATATCTCGAGGGAGAACCATTCGTCAATTCATTTTTGGCGTAAT
TATTGTACCGGTTCTAGTAATCTCTGCATGGTTTACTGTTTTTGGTACTACTGCAGGAGATATGCAGCAACAAGGAATTG
TTGACCTAACTCAATTTCCAACTGAACTCGTTTTATTTAATATGTTTGATTACCTACCTTTATCAACCATCATCTCTTTA
CTGGCAATATTTTTATTGGTTATTTTCTTTATCACGTCTGCGGATTCAGCGACATTTGTGTTAGGTATGCAGTCAACCGG
AGGATCACTTTCACCCCCGAATTCTGTGAAAGTCGCACTTGGTTTATCTATTTCTGCGGTAGCATTAATTCTTTTATATG
TAGGCGGACTTAGCTCCCTGCAAAATACAGTTATTGTTGCAGCATTACCATTTTCCTTTGTTATTATATTAATGGTTATT
TCTTTATATAAAGCCTTAAAAGAGGATGCTTACATCAAAAAAAGGAAAACATCATTAAAGAAAAATGAAGAAGCATAA

Upstream 100 bases:

>100_bases
TGTACGCGGTTCATAATTCAAAAGAAAAGTTTAAAAATATTCAAAAAAGGGTAGTTTATTATATCTATTAACTACGCTCT
TCCATACGAAAGGGGAACAC

Downstream 100 bases:

>100_bases
AGCTTCACTTCATAAGATTCTACCTCTTAAAGGGTAGCGATATTTATCAGATAGAAAAAGTGAAGGCAAATTTTACAGCC
TTCACTTTTTCTATGCTTCG

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MKKVTNVFYFAVILVGIMVALGAIFPVKFEQISQSVQAWVSSTFGWYYLLLMSVLVAVALFIAVSPYGNIRLGKDSDRPD
FSNISWVAMLFAAGIGIGLVFYGASEPLAHFMIDAPLSEPGTAQAFKEALSHSFFHWGIHGWSMYGLVALAIAYFQFRKD
EPGLISVTLKPLFGDRMNGGFGKAIDALAIFATVVGVATSLGLGAIQINAGLSHLFDLPVNIGVQATIILIVTVLFIISA
WSGLNKGIKTLSNINLILALILFIFVLAVGPTLLIFNMFSESIGLYFQNFIQMSFRSAPLASDNRAWLDIWTIFYWAWWI
SWAPFVGIFVARISRGRTIRQFIFGVIIVPVLVISAWFTVFGTTAGDMQQQGIVDLTQFPTELVLFNMFDYLPLSTIISL
LAIFLLVIFFITSADSATFVLGMQSTGGSLSPPNSVKVALGLSISAVALILLYVGGLSSLQNTVIVAALPFSFVIILMVI
SLYKALKEDAYIKKRKTSLKKNEEA

Sequences:

>Translated_505_residues
MKKVTNVFYFAVILVGIMVALGAIFPVKFEQISQSVQAWVSSTFGWYYLLLMSVLVAVALFIAVSPYGNIRLGKDSDRPD
FSNISWVAMLFAAGIGIGLVFYGASEPLAHFMIDAPLSEPGTAQAFKEALSHSFFHWGIHGWSMYGLVALAIAYFQFRKD
EPGLISVTLKPLFGDRMNGGFGKAIDALAIFATVVGVATSLGLGAIQINAGLSHLFDLPVNIGVQATIILIVTVLFIISA
WSGLNKGIKTLSNINLILALILFIFVLAVGPTLLIFNMFSESIGLYFQNFIQMSFRSAPLASDNRAWLDIWTIFYWAWWI
SWAPFVGIFVARISRGRTIRQFIFGVIIVPVLVISAWFTVFGTTAGDMQQQGIVDLTQFPTELVLFNMFDYLPLSTIISL
LAIFLLVIFFITSADSATFVLGMQSTGGSLSPPNSVKVALGLSISAVALILLYVGGLSSLQNTVIVAALPFSFVIILMVI
SLYKALKEDAYIKKRKTSLKKNEEA
>Mature_505_residues
MKKVTNVFYFAVILVGIMVALGAIFPVKFEQISQSVQAWVSSTFGWYYLLLMSVLVAVALFIAVSPYGNIRLGKDSDRPD
FSNISWVAMLFAAGIGIGLVFYGASEPLAHFMIDAPLSEPGTAQAFKEALSHSFFHWGIHGWSMYGLVALAIAYFQFRKD
EPGLISVTLKPLFGDRMNGGFGKAIDALAIFATVVGVATSLGLGAIQINAGLSHLFDLPVNIGVQATIILIVTVLFIISA
WSGLNKGIKTLSNINLILALILFIFVLAVGPTLLIFNMFSESIGLYFQNFIQMSFRSAPLASDNRAWLDIWTIFYWAWWI
SWAPFVGIFVARISRGRTIRQFIFGVIIVPVLVISAWFTVFGTTAGDMQQQGIVDLTQFPTELVLFNMFDYLPLSTIISL
LAIFLLVIFFITSADSATFVLGMQSTGGSLSPPNSVKVALGLSISAVALILLYVGGLSSLQNTVIVAALPFSFVIILMVI
SLYKALKEDAYIKKRKTSLKKNEEA

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=471, Percent_Identity=39.4904458598726, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1788102, Length=450, Percent_Identity=33.1111111111111, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786224, Length=496, Percent_Identity=29.6370967741936, Blast_Score=212, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 55213; Mature: 55213

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVTNVFYFAVILVGIMVALGAIFPVKFEQISQSVQAWVSSTFGWYYLLLMSVLVAVAL
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAVSPYGNIRLGKDSDRPDFSNISWVAMLFAAGIGIGLVFYGASEPLAHFMIDAPLSEP
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHEECCCCCC
GTAQAFKEALSHSFFHWGIHGWSMYGLVALAIAYFQFRKDEPGLISVTLKPLFGDRMNGG
CHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHCCCCCCC
FGKAIDALAIFATVVGVATSLGLGAIQINAGLSHLFDLPVNIGVQATIILIVTVLFIISA
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
WSGLNKGIKTLSNINLILALILFIFVLAVGPTLLIFNMFSESIGLYFQNFIQMSFRSAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ASDNRAWLDIWTIFYWAWWISWAPFVGIFVARISRGRTIRQFIFGVIIVPVLVISAWFTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FGTTAGDMQQQGIVDLTQFPTELVLFNMFDYLPLSTIISLLAIFLLVIFFITSADSATFV
HCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
LGMQSTGGSLSPPNSVKVALGLSISAVALILLYVGGLSSLQNTVIVAALPFSFVIILMVI
EEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHEEEHHHHHHHHHHHHH
SLYKALKEDAYIKKRKTSLKKNEEA
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKVTNVFYFAVILVGIMVALGAIFPVKFEQISQSVQAWVSSTFGWYYLLLMSVLVAVAL
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAVSPYGNIRLGKDSDRPDFSNISWVAMLFAAGIGIGLVFYGASEPLAHFMIDAPLSEP
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHEECCCCCC
GTAQAFKEALSHSFFHWGIHGWSMYGLVALAIAYFQFRKDEPGLISVTLKPLFGDRMNGG
CHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHCCCCCCC
FGKAIDALAIFATVVGVATSLGLGAIQINAGLSHLFDLPVNIGVQATIILIVTVLFIISA
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
WSGLNKGIKTLSNINLILALILFIFVLAVGPTLLIFNMFSESIGLYFQNFIQMSFRSAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ASDNRAWLDIWTIFYWAWWISWAPFVGIFVARISRGRTIRQFIFGVIIVPVLVISAWFTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FGTTAGDMQQQGIVDLTQFPTELVLFNMFDYLPLSTIISLLAIFLLVIFFITSADSATFV
HCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
LGMQSTGGSLSPPNSVKVALGLSISAVALILLYVGGLSSLQNTVIVAALPFSFVIILMVI
EEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHEEEHHHHHHHHHHHHH
SLYKALKEDAYIKKRKTSLKKNEEA
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]