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The map label for this gene is lytE [H]

Identifier: 23097729

GI number: 23097729

Start: 306924

End: 307754

Strand: Direct

Name: lytE [H]

Synonym: OB0274

Alternate gene names: 23097729

Gene position: 306924-307754 (Clockwise)

Preceding gene: 23097728

Following gene: 23097733

Centisome position: 8.45

GC content: 39.83

Gene sequence:

>831_bases
TTGGCACAGACCAATCTATCAGCTAAGAAATATGTGCTTTCTACAGCACTTGTTACTTCATTAGCTCTAACACCTGTTTT
TGCAGGAAGTGTGTTTGCTAATGCTGGAGCAGGAGCGTCGGAGGGAGATATACCTGCTAGCGAAAGTAACCTACCGAACG
CAACAGAGCAAGAAGAAACTTCACCTAGTGTGGGACTAATTCAGCGCGGAGATGTAAGTTCTGCAGTAGAAGATTTGCAA
GAAGAATTACAAGATCAAGGTTATTATACATATAACATTGATGGAATCTTTGGCCCAATTACTGAAGAAGCAGTAAGAGA
ATACCAAGCAGACCAAGACTTACAAGTGGATGGTATTGTAGGACCAAATACGAAAGACGCGTTGGCAGTACAAAATGAAA
ATTCTGATGAAGAATTGAACATTGTAGAAAAAGACGAAGATGAAAATACATCAGAGTCTGGAGACATCCAATCTGATATT
GTTGCTGCAGCAGAGAGTGTTGTAGGTACTCCATATGTATGGGGTGGAACAACTACAGATGGTATGGATAGTAGTGGATT
TATTAACTATGTATTCGATCAAGTAGATATTGATATTTCTCGTACGCATAGTGAAATGTGGGAGAACGATGGAGTACATG
TAGACTCTCCTGAAGTTGGAGATGTTGTATTCTTTGAAGGAACTTATGACACTGAAGGAGCTTCTCACAGTGGTATCTAT
ATTGGTGACGGTCAAATGATTCACGCTGGAAATGATGGTGTAAGTGTTGCTGATTTCACTATTGATTACTGGCAAGATCA
TTACATTGGCGCGAAATCTTTCACTGAATAA

Upstream 100 bases:

>100_bases
TTTCATATGATAGATTGGGTACAGTGGTAAAATATGATGGATTTTGTCGATAATTATCATATTTTAACTAGTATAGTAGA
GAAATAAAGGAGGAATAACG

Downstream 100 bases:

>100_bases
TACAATCTAAAATTAGTATGATTACTCATTGAAAAGAGGCTAGTAAAATCTAGCCTCTTTCTTTATAGCTTTAGTAGCAA
TGAGTGTGTTATCTTTTTTC

Product: cell wall associated protein

Products: NA

Alternate protein names: Cell wall-associated polypeptide CWBP33; Gamma-D-glutamate-meso-diaminopimelate muropeptidase lytE; Phosphatase-associated protein papQ [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAQTNLSAKKYVLSTALVTSLALTPVFAGSVFANAGAGASEGDIPASESNLPNATEQEETSPSVGLIQRGDVSSAVEDLQ
EELQDQGYYTYNIDGIFGPITEEAVREYQADQDLQVDGIVGPNTKDALAVQNENSDEELNIVEKDEDENTSESGDIQSDI
VAAAESVVGTPYVWGGTTTDGMDSSGFINYVFDQVDIDISRTHSEMWENDGVHVDSPEVGDVVFFEGTYDTEGASHSGIY
IGDGQMIHAGNDGVSVADFTIDYWQDHYIGAKSFTE

Sequences:

>Translated_276_residues
MAQTNLSAKKYVLSTALVTSLALTPVFAGSVFANAGAGASEGDIPASESNLPNATEQEETSPSVGLIQRGDVSSAVEDLQ
EELQDQGYYTYNIDGIFGPITEEAVREYQADQDLQVDGIVGPNTKDALAVQNENSDEELNIVEKDEDENTSESGDIQSDI
VAAAESVVGTPYVWGGTTTDGMDSSGFINYVFDQVDIDISRTHSEMWENDGVHVDSPEVGDVVFFEGTYDTEGASHSGIY
IGDGQMIHAGNDGVSVADFTIDYWQDHYIGAKSFTE
>Mature_275_residues
AQTNLSAKKYVLSTALVTSLALTPVFAGSVFANAGAGASEGDIPASESNLPNATEQEETSPSVGLIQRGDVSSAVEDLQE
ELQDQGYYTYNIDGIFGPITEEAVREYQADQDLQVDGIVGPNTKDALAVQNENSDEELNIVEKDEDENTSESGDIQSDIV
AAAESVVGTPYVWGGTTTDGMDSSGFINYVFDQVDIDISRTHSEMWENDGVHVDSPEVGDVVFFEGTYDTEGASHSGIYI
GDGQMIHAGNDGVSVADFTIDYWQDHYIGAKSFTE

Specific function: Cleaves gamma-D-glutamate-meso-diaminopimelate bonds. Cell wall hydrolase involved in cell autolysis [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted, cell wall. Note=LysM repeats are thought to be involved in peptidoglycan binding [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=115, Percent_Identity=38.2608695652174, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786421, Length=194, Percent_Identity=26.8041237113402, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788501, Length=132, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 29554; Mature: 29423

Theoretical pI: Translated: 3.52; Mature: 3.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTNLSAKKYVLSTALVTSLALTPVFAGSVFANAGAGASEGDIPASESNLPNATEQEET
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCC
SPSVGLIQRGDVSSAVEDLQEELQDQGYYTYNIDGIFGPITEEAVREYQADQDLQVDGIV
CCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCEECCEE
GPNTKDALAVQNENSDEELNIVEKDEDENTSESGDIQSDIVAAAESVVGTPYVWGGTTTD
CCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCC
GMDSSGFINYVFDQVDIDISRTHSEMWENDGVHVDSPEVGDVVFFEGTYDTEGASHSGIY
CCCCCCCEEEEHHHHCCCHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCCCCCEE
IGDGQMIHAGNDGVSVADFTIDYWQDHYIGAKSFTE
EECCEEEEECCCCCEEEEEEEEHHHCCCCCCCCCCC
>Mature Secondary Structure 
AQTNLSAKKYVLSTALVTSLALTPVFAGSVFANAGAGASEGDIPASESNLPNATEQEET
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCC
SPSVGLIQRGDVSSAVEDLQEELQDQGYYTYNIDGIFGPITEEAVREYQADQDLQVDGIV
CCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCEECCEE
GPNTKDALAVQNENSDEELNIVEKDEDENTSESGDIQSDIVAAAESVVGTPYVWGGTTTD
CCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCC
GMDSSGFINYVFDQVDIDISRTHSEMWENDGVHVDSPEVGDVVFFEGTYDTEGASHSGIY
CCCCCCCEEEEHHHHCCCHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCCCCCEE
IGDGQMIHAGNDGVSVADFTIDYWQDHYIGAKSFTE
EECCEEEEECCCCCEEEEEEEEHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9457885; 9579061; 9384377; 8045898 [H]