Click here to switch to the map view.

The map label for this gene is ybbD [H]

Identifier: 23097725

GI number: 23097725

Start: 302049

End: 303323

Strand: Direct

Name: ybbD [H]

Synonym: OB0270

Alternate gene names: 23097725

Gene position: 302049-303323 (Clockwise)

Preceding gene: 23097724

Following gene: 23097726

Centisome position: 8.32

GC content: 37.49

Gene sequence:

>1275_bases
ATGAAATGGATAAGTAGTATTGTCATTATGGCGGTTATTACCGTTGGAGTGGTTTATTATTATTTACAATCAGGCGACGA
AAAAACTAATCATTCCAATCATAATACTTCAATGGGTGGAACATTGGATGTATCGAGTTTGGTAGATGCGATGAGTTTAG
ATGAAAAGATTGGGCAATTGATCGTTGGTGGAATTGACGGAACAGAAATAAATACGGATACGAAAGACATGATTGAAAAT
TATCATGTTGGAGGAGTTATTCTGTTTGCAGACAATATTGAAAGCAAGGCACAAACTGTAAATCTTATGAATGACATAAA
ACAGGTGAATGAGAATAATCCACATCCATTGCTTCTTGGGGTTGATGAAGAAGGTGGAAGCGTAACAAGAATGCCAGACG
AGATCACAAGTCTCCCACCAAGTGGATCTATAGGAAAAGCACAAGATGAGGAACTTGCTTTTGAAGTTGGTATTCTTCTT
GGACAGCAAATGCGAGGATTAGGATTCAATTTAAATTTCGCTCCGGTTCTTGATGTGAATAGTAATCCAGATAATCCTGT
AATTGGCGATCGTTCCTTTGGTGATAGTCCAGACATCGTGACTGATATGGGGATTCAATCCATGAAAGGAATTCAAAGTG
AGGGGATTATCTCTGTCGTCAAACACTTTCCTGGGCATGGAGATACAAGTGAGGATTCACATTTAAAGCTTCCCAAAGTC
GATAAAAGTATAGAGGAGCTTTCAGAAGTGGAACTGGTTCCTTTTGAAAAAGCAATTTCCAAAGGAGCAGACGTGGTGAT
GACTGCACACATCTTGTTGCCACAAATAGATACTGAATATCCAGCATCTATGTCTGAAAAAATCATTTCTGGTCTATTGA
GAGAAGATATGGGGTTTGATGGAGTTGTTATCTCAGACGATTTAACAATGGGAGCAATTACGGAAAACATTAGAATAGAA
GAAGCGGCTATCCAATCTGTAAAAGCTGGCAGTGATATGGTACTTATCGCACATCATCCAGATGTAGTGGTATCCGTTCA
TAAGAAGCTAAAAGCAGCCGTGCAAAATGGTGTTATTTCTGAAGCAAAAATTGATGAAAGTGTAGAAAGAATTATTCAGC
TAAAAAGGAAATATAAACTTTCAAATGAGGAAACTCCGCAGATAGATTTTCAACGAATAAACGAAAAGGTGGAAGAGATA
CTAGATCAAGTCTCTTCCACCTTTTCGTTTGATGAATTTTTAGTATATTCCAAATTCATGTCAATTGTACTATAA

Upstream 100 bases:

>100_bases
GGTATATCTGGTTAGCAAGCAGCGATTAAAGAAAGATACTTCCGGGGAAAATGGGGAATGAGAAACATAGAATTATTTCG
ACACCTTGGAGGTAAGCAGA

Downstream 100 bases:

>100_bases
TGAAGATTAGTAAAGGTATATATACCTGTATATTTGTAAGGAGTGATTGTAATAGCTGTTTTAAAGTTAAAAGAGGTATC
CAAAAAGTACAAATCTCAAC

Product: beta-hexosaminidase

Products: N-acetylglucosamin; anhydro-N-acetylmuramic acid [C]

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKWISSIVIMAVITVGVVYYYLQSGDEKTNHSNHNTSMGGTLDVSSLVDAMSLDEKIGQLIVGGIDGTEINTDTKDMIEN
YHVGGVILFADNIESKAQTVNLMNDIKQVNENNPHPLLLGVDEEGGSVTRMPDEITSLPPSGSIGKAQDEELAFEVGILL
GQQMRGLGFNLNFAPVLDVNSNPDNPVIGDRSFGDSPDIVTDMGIQSMKGIQSEGIISVVKHFPGHGDTSEDSHLKLPKV
DKSIEELSEVELVPFEKAISKGADVVMTAHILLPQIDTEYPASMSEKIISGLLREDMGFDGVVISDDLTMGAITENIRIE
EAAIQSVKAGSDMVLIAHHPDVVVSVHKKLKAAVQNGVISEAKIDESVERIIQLKRKYKLSNEETPQIDFQRINEKVEEI
LDQVSSTFSFDEFLVYSKFMSIVL

Sequences:

>Translated_424_residues
MKWISSIVIMAVITVGVVYYYLQSGDEKTNHSNHNTSMGGTLDVSSLVDAMSLDEKIGQLIVGGIDGTEINTDTKDMIEN
YHVGGVILFADNIESKAQTVNLMNDIKQVNENNPHPLLLGVDEEGGSVTRMPDEITSLPPSGSIGKAQDEELAFEVGILL
GQQMRGLGFNLNFAPVLDVNSNPDNPVIGDRSFGDSPDIVTDMGIQSMKGIQSEGIISVVKHFPGHGDTSEDSHLKLPKV
DKSIEELSEVELVPFEKAISKGADVVMTAHILLPQIDTEYPASMSEKIISGLLREDMGFDGVVISDDLTMGAITENIRIE
EAAIQSVKAGSDMVLIAHHPDVVVSVHKKLKAAVQNGVISEAKIDESVERIIQLKRKYKLSNEETPQIDFQRINEKVEEI
LDQVSSTFSFDEFLVYSKFMSIVL
>Mature_424_residues
MKWISSIVIMAVITVGVVYYYLQSGDEKTNHSNHNTSMGGTLDVSSLVDAMSLDEKIGQLIVGGIDGTEINTDTKDMIEN
YHVGGVILFADNIESKAQTVNLMNDIKQVNENNPHPLLLGVDEEGGSVTRMPDEITSLPPSGSIGKAQDEELAFEVGILL
GQQMRGLGFNLNFAPVLDVNSNPDNPVIGDRSFGDSPDIVTDMGIQSMKGIQSEGIISVVKHFPGHGDTSEDSHLKLPKV
DKSIEELSEVELVPFEKAISKGADVVMTAHILLPQIDTEYPASMSEKIISGLLREDMGFDGVVISDDLTMGAITENIRIE
EAAIQSVKAGSDMVLIAHHPDVVVSVHKKLKAAVQNGVISEAKIDESVERIIQLKRKYKLSNEETPQIDFQRINEKVEEI
LDQVSSTFSFDEFLVYSKFMSIVL

Specific function: Hydrolyses the beta-1,4-glycosidic bond between N-acetylglucosamine and anhydro-N-acetylmuramic acid in cell wall degradation products following their importation into the cell during the process for recycling cell wall muropeptides [C]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=325, Percent_Identity=30.4615384615385, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1788453, Length=398, Percent_Identity=24.1206030150754, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR002772
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3; PF01915 Glyco_hydro_3_C [H]

EC number: NA

Molecular weight: Translated: 46283; Mature: 46283

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWISSIVIMAVITVGVVYYYLQSGDEKTNHSNHNTSMGGTLDVSSLVDAMSLDEKIGQL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHE
IVGGIDGTEINTDTKDMIENYHVGGVILFADNIESKAQTVNLMNDIKQVNENNPHPLLLG
EECCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
VDEEGGSVTRMPDEITSLPPSGSIGKAQDEELAFEVGILLGQQMRGLGFNLNFAPVLDVN
ECCCCCCEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
SNPDNPVIGDRSFGDSPDIVTDMGIQSMKGIQSEGIISVVKHFPGHGDTSEDSHLKLPKV
CCCCCCCCCCCCCCCCCCHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEECCCH
DKSIEELSEVELVPFEKAISKGADVVMTAHILLPQIDTEYPASMSEKIISGLLREDMGFD
HHHHHHHHHCCCCCHHHHHHCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
GVVISDDLTMGAITENIRIEEAAIQSVKAGSDMVLIAHHPDVVVSVHKKLKAAVQNGVIS
CEEEECCCEEHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCC
EAKIDESVERIIQLKRKYKLSNEETPQIDFQRINEKVEEILDQVSSTFSFDEFLVYSKFM
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
SIVL
HHHC
>Mature Secondary Structure
MKWISSIVIMAVITVGVVYYYLQSGDEKTNHSNHNTSMGGTLDVSSLVDAMSLDEKIGQL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHE
IVGGIDGTEINTDTKDMIENYHVGGVILFADNIESKAQTVNLMNDIKQVNENNPHPLLLG
EECCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
VDEEGGSVTRMPDEITSLPPSGSIGKAQDEELAFEVGILLGQQMRGLGFNLNFAPVLDVN
ECCCCCCEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
SNPDNPVIGDRSFGDSPDIVTDMGIQSMKGIQSEGIISVVKHFPGHGDTSEDSHLKLPKV
CCCCCCCCCCCCCCCCCCHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEECCCH
DKSIEELSEVELVPFEKAISKGADVVMTAHILLPQIDTEYPASMSEKIISGLLREDMGFD
HHHHHHHHHCCCCCHHHHHHCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
GVVISDDLTMGAITENIRIEEAAIQSVKAGSDMVLIAHHPDVVVSVHKKLKAAVQNGVIS
CEEEECCCEEHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCC
EAKIDESVERIIQLKRKYKLSNEETPQIDFQRINEKVEEILDQVSSTFSFDEFLVYSKFM
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
SIVL
HHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cell Wall [C]

Specific reaction: Cell Wall = N-acetylglucosamin + anhydro-N-acetylmuramic acid [C]

General reaction: Hydrolyse [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8011666; 9274029; 9384377 [H]