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The map label for this gene is cwlD [H]

Identifier: 23097650

GI number: 23097650

Start: 209362

End: 210093

Strand: Direct

Name: cwlD [H]

Synonym: OB0195

Alternate gene names: 23097650

Gene position: 209362-210093 (Clockwise)

Preceding gene: 23097648

Following gene: 23097651

Centisome position: 5.77

GC content: 37.3

Gene sequence:

>732_bases
ATGGGAAGAAAAAAGAAAATTGCTTTTTGGCTATTAGGTGTATTTTTACTCGCGTACTTATTACAATACCCAATAAATGA
GACAAATATGACAGTAAATCAAGGGTGGACGTTACCCCTTACCGGAAAAACAATTGTAATCGATCCTGGCCATGGTGGAC
CTGACGGAGGGGCAGTTGGATCCGATGATACAGAAGAGAAAGATATTGCTTTACAAGTATCGAAACTAATTCAATCTTAT
TTGCAACAACAAGGAGCACTTGTTTATTTAACTCGTGAACAGGATAAGGATTTAGCCGCTGAAGAAACAAGTGGACTAGC
TCGAAGAAAATCTGAAGATATTCGAAATCGTTTGAAGTTTATTCACGATAAGGAACCGGACTTTTTCCTTTCTTTGCATT
TAAATGCATTACCATCGACACAATGGAGTGGAGCACAAACGTTTTATTATCCAACAAAAGATGAGAATAAGCATTTGGCC
ACGATGATTCAACAAGAAATAATACGAAACTTAGAAAACACGAATCGGTCACCATTAGCATTAAATAGCATGTATTTATT
AAAGCATGCTGAAGTACCAGGAGCTTTGGTAGAAATTGGATTTTTGTCTAATGTCGAGGAAAGAGAATTGTTAAAAGACG
AAGATTATCAACGAAAAATGGCAGCAAGTATTTATGAGGGAATTTTACGATATGCGACAGAGGAGCCTGAAGAACAGGAG
GAGTCAGACTGA

Upstream 100 bases:

>100_bases
CCTACCTCGTAAATAGCATACCATAAATGTTAAGTCATAAACTGCAATCTTGTCCCATAAATTTAGTAAAAGAGAAGTTA
AAGGAAGGAGCAAGAACTAT

Downstream 100 bases:

>100_bases
AACCGTCACCAAATAGCATATTACTTAGATTTTCTGTGTTTTGTGTTATACTAGCAGGTGGATACAAACACAAGGGATGG
TGATGTACGATGCTAACAAA

Product: germination specific N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MGRKKKIAFWLLGVFLLAYLLQYPINETNMTVNQGWTLPLTGKTIVIDPGHGGPDGGAVGSDDTEEKDIALQVSKLIQSY
LQQQGALVYLTREQDKDLAAEETSGLARRKSEDIRNRLKFIHDKEPDFFLSLHLNALPSTQWSGAQTFYYPTKDENKHLA
TMIQQEIIRNLENTNRSPLALNSMYLLKHAEVPGALVEIGFLSNVEERELLKDEDYQRKMAASIYEGILRYATEEPEEQE
ESD

Sequences:

>Translated_243_residues
MGRKKKIAFWLLGVFLLAYLLQYPINETNMTVNQGWTLPLTGKTIVIDPGHGGPDGGAVGSDDTEEKDIALQVSKLIQSY
LQQQGALVYLTREQDKDLAAEETSGLARRKSEDIRNRLKFIHDKEPDFFLSLHLNALPSTQWSGAQTFYYPTKDENKHLA
TMIQQEIIRNLENTNRSPLALNSMYLLKHAEVPGALVEIGFLSNVEERELLKDEDYQRKMAASIYEGILRYATEEPEEQE
ESD
>Mature_242_residues
GRKKKIAFWLLGVFLLAYLLQYPINETNMTVNQGWTLPLTGKTIVIDPGHGGPDGGAVGSDDTEEKDIALQVSKLIQSYL
QQQGALVYLTREQDKDLAAEETSGLARRKSEDIRNRLKFIHDKEPDFFLSLHLNALPSTQWSGAQTFYYPTKDENKHLAT
MIQQEIIRNLENTNRSPLALNSMYLLKHAEVPGALVEIGFLSNVEERELLKDEDYQRKMAASIYEGILRYATEEPEEQEE
SD

Specific function: Cell-Wall Hydrolase Probably Involved In Cell-Wall Hydrolysis, Septation Or Recycling (By Similarity). [C]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=267, Percent_Identity=28.4644194756554, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788776, Length=233, Percent_Identity=24.8927038626609, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27605; Mature: 27473

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKKKIAFWLLGVFLLAYLLQYPINETNMTVNQGWTLPLTGKTIVIDPGHGGPDGGAVG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCEEEEECCCCCCCCCCCC
SDDTEEKDIALQVSKLIQSYLQQQGALVYLTREQDKDLAAEETSGLARRKSEDIRNRLKF
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IHDKEPDFFLSLHLNALPSTQWSGAQTFYYPTKDENKHLATMIQQEIIRNLENTNRSPLA
HCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
LNSMYLLKHAEVPGALVEIGFLSNVEERELLKDEDYQRKMAASIYEGILRYATEEPEEQE
EHHHHHHHHCCCCCHHEEEHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
ESD
CCC
>Mature Secondary Structure 
GRKKKIAFWLLGVFLLAYLLQYPINETNMTVNQGWTLPLTGKTIVIDPGHGGPDGGAVG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCEEEEECCCCCCCCCCCC
SDDTEEKDIALQVSKLIQSYLQQQGALVYLTREQDKDLAAEETSGLARRKSEDIRNRLKF
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IHDKEPDFFLSLHLNALPSTQWSGAQTFYYPTKDENKHLATMIQQEIIRNLENTNRSPLA
HCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
LNSMYLLKHAEVPGALVEIGFLSNVEERELLKDEDYQRKMAASIYEGILRYATEEPEEQE
EHHHHHHHHCCCCCHHEEEHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
ESD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]