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The map label for this gene is truA

Identifier: 23097605

GI number: 23097605

Start: 158840

End: 159601

Strand: Direct

Name: truA

Synonym: OB0150

Alternate gene names: 23097605

Gene position: 158840-159601 (Clockwise)

Preceding gene: 23097604

Following gene: 23097606

Centisome position: 4.38

GC content: 37.8

Gene sequence:

>762_bases
GTGGAAAGATTAAAATGCACCGTATCGTATGATGGATCTGATTTTGCCGGATTCCAAGTACAACCAGATCACAGGACGGT
ACAAGGTGTCTTGGAAAAAGCATTGTACAACATGCATAAAGGTAAATCAATACGGATTCAAGCATCCGGTAGAACGGATA
CAGGTGTGCATGCTGTTGGACAAGTAATTCACTTCGACAGTCCACTGGAAATCCCTGAACGAAATTGGAAACAAGCACTA
AATACATTACTACCGGATGATGTGCGAATTCAAAAGGTAGAAAAGAAAACAGAAGAATTCCATGCTCGATATAGTGTGAA
AGAAAAAGAATATCACTATTTTGTCTTAAATGAAGAGGATCCTAATGTCTTTCAGAGAGGATATGTTTACCACGAACCTA
GAAAACTGGATCTCGAACGAATGCAAGAAGCCTGTCAGTATCTAGAAGGTACACATGATTTTACGAGTTTTTCTTCAGCA
AAGTCATCAGTAAAAGGATCAAAAGTTCGTACGTTATATCACGTATCTTGTCACCAAACAGGTACTAATATTGAATTTAT
CTTTCGGGGAAGCGGCTTTTTATATAATATGGTACGTATCATGATGAGTGTACTTATTGATGTTGGTAAAGGGAAGCGAA
ACCCAGAAGATATTATAGATTTGTTAGCGAGCAAAAACCGTCAACAAATAGGAAAAACAATGGCTCCACAAGGCTTGTAT
TTATGGCGAGTATCCTATGAAGAAAATGGCAATAATGTGTAA

Upstream 100 bases:

>100_bases
TGGACCTAAGGATATTAGTATTATGGTTAGCTTTGCAATTGTCATAATAGCTTTATTCTTACTGAGAAAGTAGATTAGGG
AAAGAAAGAGGTTGAAGTAA

Downstream 100 bases:

>100_bases
TAAATTATTTCACCGGCAGCTTGACTTTTACGGCTAATCGTTATATTATGGTATATGGCATTTTATTTCTAAACCATGAT
TAGCCCCGGAATCTAATTAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MERLKCTVSYDGSDFAGFQVQPDHRTVQGVLEKALYNMHKGKSIRIQASGRTDTGVHAVGQVIHFDSPLEIPERNWKQAL
NTLLPDDVRIQKVEKKTEEFHARYSVKEKEYHYFVLNEEDPNVFQRGYVYHEPRKLDLERMQEACQYLEGTHDFTSFSSA
KSSVKGSKVRTLYHVSCHQTGTNIEFIFRGSGFLYNMVRIMMSVLIDVGKGKRNPEDIIDLLASKNRQQIGKTMAPQGLY
LWRVSYEENGNNV

Sequences:

>Translated_253_residues
MERLKCTVSYDGSDFAGFQVQPDHRTVQGVLEKALYNMHKGKSIRIQASGRTDTGVHAVGQVIHFDSPLEIPERNWKQAL
NTLLPDDVRIQKVEKKTEEFHARYSVKEKEYHYFVLNEEDPNVFQRGYVYHEPRKLDLERMQEACQYLEGTHDFTSFSSA
KSSVKGSKVRTLYHVSCHQTGTNIEFIFRGSGFLYNMVRIMMSVLIDVGKGKRNPEDIIDLLASKNRQQIGKTMAPQGLY
LWRVSYEENGNNV
>Mature_253_residues
MERLKCTVSYDGSDFAGFQVQPDHRTVQGVLEKALYNMHKGKSIRIQASGRTDTGVHAVGQVIHFDSPLEIPERNWKQAL
NTLLPDDVRIQKVEKKTEEFHARYSVKEKEYHYFVLNEEDPNVFQRGYVYHEPRKLDLERMQEACQYLEGTHDFTSFSSA
KSSVKGSKVRTLYHVSCHQTGTNIEFIFRGSGFLYNMVRIMMSVLIDVGKGKRNPEDIIDLLASKNRQQIGKTMAPQGLY
LWRVSYEENGNNV

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=34.1818181818182, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI199559785, Length=280, Percent_Identity=28.2142857142857, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1788657, Length=248, Percent_Identity=33.8709677419355, Blast_Score=147, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71985046, Length=255, Percent_Identity=25.8823529411765, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321112, Length=246, Percent_Identity=28.4552845528455, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI116007752, Length=307, Percent_Identity=25.7328990228013, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362146, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_OCEIH (Q8CXP1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691071.1
- ProteinModelPortal:   Q8CXP1
- GeneID:   1015246
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0150
- NMPDR:   fig|221109.1.peg.151
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- BioCyc:   OIHE221109:OB0150-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29170; Mature: 29170

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLKCTVSYDGSDFAGFQVQPDHRTVQGVLEKALYNMHKGKSIRIQASGRTDTGVHAVG
CCCEEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
QVIHFDSPLEIPERNWKQALNTLLPDDVRIQKVEKKTEEFHARYSVKEKEYHYFVLNEED
HHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
PNVFQRGYVYHEPRKLDLERMQEACQYLEGTHDFTSFSSAKSSVKGSKVRTLYHVSCHQT
CCHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCC
GTNIEFIFRGSGFLYNMVRIMMSVLIDVGKGKRNPEDIIDLLASKNRQQIGKTMAPQGLY
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCEE
LWRVSYEENGNNV
EEEEEECCCCCCC
>Mature Secondary Structure
MERLKCTVSYDGSDFAGFQVQPDHRTVQGVLEKALYNMHKGKSIRIQASGRTDTGVHAVG
CCCEEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
QVIHFDSPLEIPERNWKQALNTLLPDDVRIQKVEKKTEEFHARYSVKEKEYHYFVLNEED
HHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
PNVFQRGYVYHEPRKLDLERMQEACQYLEGTHDFTSFSSAKSSVKGSKVRTLYHVSCHQT
CCHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCC
GTNIEFIFRGSGFLYNMVRIMMSVLIDVGKGKRNPEDIIDLLASKNRQQIGKTMAPQGLY
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCEE
LWRVSYEENGNNV
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376