BacMap

Definition	Oceanobacillus iheyensis HTE831, complete genome.
Accession	NC_004193
Length	3,630,528

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The map label for this gene is tuf

Identifier: 23097572

GI number: 23097572

Start: 139729

End: 140916

Strand: Direct

Name: tuf

Synonym: OB0117

Alternate gene names: 23097572

Gene position: 139729-140916 (Clockwise)

Preceding gene: 23097571

Following gene: 23097573

Centisome position: 3.85

GC content: 42.51

Gene sequence:

>1188_bases
ATGGCTAAAGAAAAATTCGATCGCTCCAAAAGTCACGTTAACGTTGGTACTTTAGGACACGTTGACCATGGTAAAACTAC
ATTAACAGCTGCTATCACTACTGTACTTGCAAAACACGGCGGTGGAGAAGCACGTGCGTATGACCAAATTGATGGTGCGC
CTGAAGAAAGAGAACGTGGTATCACAATCTCAACTGCACACGTAGAGTATGAAACTGAAACTCGTCACTATGCACACGTT
GACTGCCCAGGTCACGCTGACTATGTTAAAAACATGATCACTGGTGCTGCACAAATGGACGGAGCTATCCTTGTAGTATC
TGCTGCTGATGGCCCAATGCCACAAACTCGTGAGCACATCCTTCTATCTCGTAACGTTGGGGTACCTGCTTTCGTAGTAT
TCCTTAACAAAACAGATATGGTTGACGATGAAGAGCTACTTGAATTAGTAGAAATGGAAGTTCGTGACCTATTAACTGAG
TACGATTTCCCTGGTGACGATCTACCAGTAATCAAAGGTTCTGCACTTAAAGCTCTAGAAGGCGTTGCAGAATACGAAGA
AAGAATTCTTGAGTTAATGGCTGCAGTTGATGAGTATATCCCAACTCCAGAACGTGACAAAGAAAAACCATTCATGATGC
CAGTTGAGGATGTATTCTCAATCACTGGTCGTGGAACAGTTGCAACTGGACGTGTTGAGCGTGGAGAAGTTAAAGTTGGA
GATGAAGTTGAAATCATCGGTCTTGCTGAAGATGCAAGCAAAACAACTGTAACTGGTGTTGAAATGTTCCGTAAGCTTCT
AGATTATGCTGAAGCTGGAGATAACATTGGTGCACTTCTTCGTGGGGTTTCTCGTGAAGACATCAACCGTGGTCAAGTAT
TAGCTAAACCAGGTTCAATCACTCCACATACTAACTTCAAAGCTGAAGTTTATGTATTATCAAAAGAAGAAGGTGGACGT
CATACTCCATTCTTCTCTAACTACCGTCCACAGTTCTACTTCCGTACTACGGACGTAACTGGTGTTATTGAATTACCAGA
AGGAACTGAAATGGTAATGCCTGGTGATAACATTGAAATGACAGTAGAACTTATTTCTCCAATCGCGATTGAAGACGGAA
CTCGTTTTTCAATCCGTGAAGGTGGACGTACTGTAGGTTCTGGCGTTGTTTCTTCTATCCAAAAATAA

Upstream 100 bases:

>100_bases
TTATCAATCTAAAGTATAACTTTTAGTAAAGGCTGAGAAATACAAATCTGTATTTCTTGCCACTAATACAAATGGTATTT
AATTAAAGGAGGAACTATAA

Downstream 100 bases:

>100_bases
GTAATTAAAATAAAAACAGACAATGGAATTCCATTGTCTGTTTCTTTATGTGTGTTTTCTTTCCTAACCTATACGAATAA
TACTTACTGTTGTTTATATC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKEKFDRSKSHVNVGTLGHVDHGKTTLTAAITTVLAKHGGGEARAYDQIDGAPEERERGITISTAHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRNVGVPAFVVFLNKTDMVDDEELLELVEMEVRDLLTE
YDFPGDDLPVIKGSALKALEGVAEYEERILELMAAVDEYIPTPERDKEKPFMMPVEDVFSITGRGTVATGRVERGEVKVG
DEVEIIGLAEDASKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREDINRGQVLAKPGSITPHTNFKAEVYVLSKEEGGR
HTPFFSNYRPQFYFRTTDVTGVIELPEGTEMVMPGDNIEMTVELISPIAIEDGTRFSIREGGRTVGSGVVSSIQK

Sequences:

>Translated_395_residues
MAKEKFDRSKSHVNVGTLGHVDHGKTTLTAAITTVLAKHGGGEARAYDQIDGAPEERERGITISTAHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRNVGVPAFVVFLNKTDMVDDEELLELVEMEVRDLLTE
YDFPGDDLPVIKGSALKALEGVAEYEERILELMAAVDEYIPTPERDKEKPFMMPVEDVFSITGRGTVATGRVERGEVKVG
DEVEIIGLAEDASKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREDINRGQVLAKPGSITPHTNFKAEVYVLSKEEGGR
HTPFFSNYRPQFYFRTTDVTGVIELPEGTEMVMPGDNIEMTVELISPIAIEDGTRFSIREGGRTVGSGVVSSIQK
>Mature_394_residues
AKEKFDRSKSHVNVGTLGHVDHGKTTLTAAITTVLAKHGGGEARAYDQIDGAPEERERGITISTAHVEYETETRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRNVGVPAFVVFLNKTDMVDDEELLELVEMEVRDLLTEY
DFPGDDLPVIKGSALKALEGVAEYEERILELMAAVDEYIPTPERDKEKPFMMPVEDVFSITGRGTVATGRVERGEVKVGD
EVEIIGLAEDASKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREDINRGQVLAKPGSITPHTNFKAEVYVLSKEEGGRH
TPFFSNYRPQFYFRTTDVTGVIELPEGTEMVMPGDNIEMTVELISPIAIEDGTRFSIREGGRTVGSGVVSSIQK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=393, Percent_Identity=57.7608142493639, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI223555963, Length=442, Percent_Identity=30.5429864253394, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI5729864, Length=442, Percent_Identity=30.5429864253394, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI4503475, Length=454, Percent_Identity=28.6343612334802, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4503471, Length=452, Percent_Identity=28.5398230088496, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI54607086, Length=333, Percent_Identity=30.03003003003, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI46094014, Length=332, Percent_Identity=27.710843373494, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI194018522, Length=346, Percent_Identity=27.4566473988439, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI194018520, Length=345, Percent_Identity=27.536231884058, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI194097354, Length=345, Percent_Identity=27.536231884058, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI4503507, Length=347, Percent_Identity=27.9538904899135, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157426893, Length=252, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI45593140, Length=429, Percent_Identity=23.0769230769231, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=76.3358778625954, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=76.3358778625954, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI2367247, Length=371, Percent_Identity=30.7277628032345, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI48994988, Length=272, Percent_Identity=33.0882352941176, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789108, Length=320, Percent_Identity=30.625, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788922, Length=329, Percent_Identity=25.8358662613982, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=52.2613065326633, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.6692506459948, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=30.6167400881057, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=30.6167400881057, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=28.735632183908, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=28.1464530892449, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=28.1464530892449, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=453, Percent_Identity=27.1523178807947, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=29.5302013422819, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=176, Percent_Identity=34.0909090909091, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=274, Percent_Identity=27.7372262773723, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=27.0386266094421, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=394, Percent_Identity=65.7360406091371, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=450, Percent_Identity=29.5555555555556, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=450, Percent_Identity=29.5555555555556, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=346, Percent_Identity=25.4335260115607, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=311, Percent_Identity=26.3665594855305, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=29.5081967213115, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=144, Percent_Identity=34.0277777777778, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=57.6530612244898, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=57.6530612244898, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=47.6683937823834, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=29.0465631929047, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=29.0465631929047, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=29.0465631929047, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=29.0465631929047, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24652838, Length=451, Percent_Identity=28.3813747228381, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17137572, Length=451, Percent_Identity=28.3813747228381, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=31.25, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI45550900, Length=343, Percent_Identity=30.0291545189504, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=30.2325581395349, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647046, Length=360, Percent_Identity=26.1111111111111, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647048, Length=360, Percent_Identity=26.1111111111111, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78706572, Length=252, Percent_Identity=26.5873015873016, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28572034, Length=172, Percent_Identity=37.2093023255814, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_OCEIH (Q8ETY4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691038.1
- ProteinModelPortal:   Q8ETY4
- SMR:   Q8ETY4
- GeneID:   1015206
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0117
- NMPDR:   fig|221109.1.peg.118
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   OIHE221109:OB0117-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43228; Mature: 43097

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKSHVNVGTLGHVDHGKTTLTAAITTVLAKHGGGEARAYDQIDGAPEERERG
CCCCHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHCCCCCHHHHCC
ITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRNVGVPAFVVFLNKTDMVDDEELLELVEMEVRDLLTEYDFPGDDLPVIKGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
GVAEYEERILELMAAVDEYIPTPERDKEKPFMMPVEDVFSITGRGTVATGRVERGEVKVG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCEECCCEEEC
DEVEIIGLAEDASKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREDINRGQVLAKPGSI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHCCCCCEEEECCCCC
TPHTNFKAEVYVLSKEEGGRHTPFFSNYRPQFYFRTTDVTGVIELPEGTEMVMPGDNIEM
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
TVELISPIAIEDGTRFSIREGGRTVGSGVVSSIQK
EEEECCCEEECCCCEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKEKFDRSKSHVNVGTLGHVDHGKTTLTAAITTVLAKHGGGEARAYDQIDGAPEERERG
CCCHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHCCCCCHHHHCC
ITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRNVGVPAFVVFLNKTDMVDDEELLELVEMEVRDLLTEYDFPGDDLPVIKGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
GVAEYEERILELMAAVDEYIPTPERDKEKPFMMPVEDVFSITGRGTVATGRVERGEVKVG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCEECCCEEEC
DEVEIIGLAEDASKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREDINRGQVLAKPGSI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHCCCCCEEEECCCCC
TPHTNFKAEVYVLSKEEGGRHTPFFSNYRPQFYFRTTDVTGVIELPEGTEMVMPGDNIEM
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
TVELISPIAIEDGTRFSIREGGRTVGSGVVSSIQK
EEEECCCEEECCCCEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376