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The map label for this gene is gltX

Identifier: 23097551

GI number: 23097551

Start: 116986

End: 118455

Strand: Direct

Name: gltX

Synonym: OB0096

Alternate gene names: 23097551

Gene position: 116986-118455 (Clockwise)

Preceding gene: 23097550

Following gene: 23097552

Centisome position: 3.22

GC content: 37.21

Gene sequence:

>1470_bases
ATGAGTAAAGAAGTTCGCGTGCGTTATGCACCAAGTCCAACAGGACATTTACATATTGGGAATGCAAGAACTGCATTATT
TAATTATTTATATGCAAAACATTTTGATGGAAAGTTTATTATCCGTACGGAGGATACAGATGATAAAAGAAATGTTGAAG
GTGGCGAAGAAAGCCAATTAAAATACTTAAAATGGTTAGGTCTACAATGGGATGAAGGCGCTGATATCGGTGGAGAGTAT
GGCCCTTATCGTCAAACAGAACGCCTTGATATTTACCAAGAGTATCTTAATGAATTATTTGCAAAAAATTTAGCTTATAA
ATGTTATATGACAGAAGATGAATTAGAAGCAGAACGGGAAGAGCAACGCCAAAATGGACAAGTTCCCAAATATTCTGGAG
CTCACCGTGACTTAACACCTGAGCAGATCGAACAATTCGAAGCTGAAGGTCGTCAACCAAGTATTCGTTTCCGAGTTCCG
GAGAATACTACGTATACATTCAATGATTTAGTTCGCGGGAAGATTACATTCGAGTCTAGTGATTTTGGAGACTGGGTTAT
TGTGAAGAAGAATGGAATTCCAACGTATAATTTTGCTGTAGCGATTGATGATCATTTAATGAAAATCAGTGATGTTTTAC
GTGGGGAAGAACATATTTCGAATACGCCGAAACAAATGATGATTTTTGATGCGTTTGGATGGGAAGCTCCTCGTTATGGA
CATATGACACTTATTCTTAACGAAGAACGCAAGAAATTAAGTAAGCGTGATGAACATATTCTTCAATTTATAGAGCAATA
TCGTAATCTTGGTTATTTACCAGAAGCGATGTTTAATTTTATTAGCTTACTTGGATGGTCTCCGGTTGGGGAAGAAGAGA
TGTTCACACAGGCACAATTGATTGAGATTTTTGATCCAGAACGTCTATCTACTTCTGCAGCAATCTTTGACCAACATAAG
CTGAAGTGGATGAATAATGAGTACATTAAAGCAGCGGATCTTGACAGAGTTATTGATCTTGCGTTACCGCATTTAATAAA
AGCTGGAAAACTGTCAGAGGACATGGATGATGAAACTCGTCAATGGGCTGAAAATGTGATCTCGCTTTACCGTGAACAGT
TACGCTATGGTGCTGAAATCGTTGAACTTACAGAACTATTTTTCCAAAAAGAAATTTCTTATGACGAAGAAGCAAAAGAA
GTACTTAACGGAGAACAAGTTCCTGAAGTACTACAAGTTTTCACGGATAAATTGATTCATTTAGAGTCATTTGACAAAGA
CGCTATAAAAGCGCAATTTAAAGCAACTCAGAAAGAAACTGGCCATCGTGGCAAGAAATTATTTATGCCTATACGTGTTG
CGACAACAGGACAAATGCATGGACCTGAACTTCCGTTTGCGATTGAGTTATTAGGTAGAGATATTATACTTAATCGATTG
GATAAACTATTGAAAGAAATGGGAGCATAA

Upstream 100 bases:

>100_bases
GAAGAAAAGTGATAGCATGAAATATGTTATCACTTTTTCATATAGAACTTATTCGATTATAAGCAAATCCATATTTTATT
CCTAAGGGAGGCAACAAGTA

Downstream 100 bases:

>100_bases
GAATTCACATTTAGGCAGATTTGTAATATATTAAGCATATAGAAATTCGATAAATAAGAGTTAATCAAATAGATAAAACG
TAGAAGAGGGAAAGTAAGAC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSKEVRVRYAPSPTGHLHIGNARTALFNYLYAKHFDGKFIIRTEDTDDKRNVEGGEESQLKYLKWLGLQWDEGADIGGEY
GPYRQTERLDIYQEYLNELFAKNLAYKCYMTEDELEAEREEQRQNGQVPKYSGAHRDLTPEQIEQFEAEGRQPSIRFRVP
ENTTYTFNDLVRGKITFESSDFGDWVIVKKNGIPTYNFAVAIDDHLMKISDVLRGEEHISNTPKQMMIFDAFGWEAPRYG
HMTLILNEERKKLSKRDEHILQFIEQYRNLGYLPEAMFNFISLLGWSPVGEEEMFTQAQLIEIFDPERLSTSAAIFDQHK
LKWMNNEYIKAADLDRVIDLALPHLIKAGKLSEDMDDETRQWAENVISLYREQLRYGAEIVELTELFFQKEISYDEEAKE
VLNGEQVPEVLQVFTDKLIHLESFDKDAIKAQFKATQKETGHRGKKLFMPIRVATTGQMHGPELPFAIELLGRDIILNRL
DKLLKEMGA

Sequences:

>Translated_489_residues
MSKEVRVRYAPSPTGHLHIGNARTALFNYLYAKHFDGKFIIRTEDTDDKRNVEGGEESQLKYLKWLGLQWDEGADIGGEY
GPYRQTERLDIYQEYLNELFAKNLAYKCYMTEDELEAEREEQRQNGQVPKYSGAHRDLTPEQIEQFEAEGRQPSIRFRVP
ENTTYTFNDLVRGKITFESSDFGDWVIVKKNGIPTYNFAVAIDDHLMKISDVLRGEEHISNTPKQMMIFDAFGWEAPRYG
HMTLILNEERKKLSKRDEHILQFIEQYRNLGYLPEAMFNFISLLGWSPVGEEEMFTQAQLIEIFDPERLSTSAAIFDQHK
LKWMNNEYIKAADLDRVIDLALPHLIKAGKLSEDMDDETRQWAENVISLYREQLRYGAEIVELTELFFQKEISYDEEAKE
VLNGEQVPEVLQVFTDKLIHLESFDKDAIKAQFKATQKETGHRGKKLFMPIRVATTGQMHGPELPFAIELLGRDIILNRL
DKLLKEMGA
>Mature_488_residues
SKEVRVRYAPSPTGHLHIGNARTALFNYLYAKHFDGKFIIRTEDTDDKRNVEGGEESQLKYLKWLGLQWDEGADIGGEYG
PYRQTERLDIYQEYLNELFAKNLAYKCYMTEDELEAEREEQRQNGQVPKYSGAHRDLTPEQIEQFEAEGRQPSIRFRVPE
NTTYTFNDLVRGKITFESSDFGDWVIVKKNGIPTYNFAVAIDDHLMKISDVLRGEEHISNTPKQMMIFDAFGWEAPRYGH
MTLILNEERKKLSKRDEHILQFIEQYRNLGYLPEAMFNFISLLGWSPVGEEEMFTQAQLIEIFDPERLSTSAAIFDQHKL
KWMNNEYIKAADLDRVIDLALPHLIKAGKLSEDMDDETRQWAENVISLYREQLRYGAEIVELTELFFQKEISYDEEAKEV
LNGEQVPEVLQVFTDKLIHLESFDKDAIKAQFKATQKETGHRGKKLFMPIRVATTGQMHGPELPFAIELLGRDIILNRLD
KLLKEMGA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=328, Percent_Identity=39.6341463414634, Blast_Score=251, Evalue=2e-66,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=34.5114345114345, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI226510925, Length=254, Percent_Identity=33.0708661417323, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786895, Length=306, Percent_Identity=25.4901960784314, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=480, Percent_Identity=31.875, Blast_Score=229, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6324540, Length=498, Percent_Identity=30.9236947791165, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=226, Percent_Identity=29.2035398230088, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=316, Percent_Identity=25.3164556962025, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=32.3293172690763, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI17864488, Length=304, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_OCEIH (Q8EU02)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691017.1
- ProteinModelPortal:   Q8EU02
- SMR:   Q8EU02
- GeneID:   1015176
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0096
- NMPDR:   fig|221109.1.peg.97
- HOGENOM:   HBG628189
- OMA:   WINNQYV
- ProtClustDB:   PRK01406
- BioCyc:   OIHE221109:OB0096-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56905; Mature: 56774

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEVRVRYAPSPTGHLHIGNARTALFNYLYAKHFDGKFIIRTEDTDDKRNVEGGEESQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHH
KYLKWLGLQWDEGADIGGEYGPYRQTERLDIYQEYLNELFAKNLAYKCYMTEDELEAERE
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHH
EQRQNGQVPKYSGAHRDLTPEQIEQFEAEGRQPSIRFRVPENTTYTFNDLVRGKITFESS
HHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCEEHHHHEEEEEEECCC
DFGDWVIVKKNGIPTYNFAVAIDDHLMKISDVLRGEEHISNTPKQMMIFDAFGWEAPRYG
CCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCC
HMTLILNEERKKLSKRDEHILQFIEQYRNLGYLPEAMFNFISLLGWSPVGEEEMFTQAQL
CEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IEIFDPERLSTSAAIFDQHKLKWMNNEYIKAADLDRVIDLALPHLIKAGKLSEDMDDETR
HHHCCCCHHCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
QWAENVISLYREQLRYGAEIVELTELFFQKEISYDEEAKEVLNGEQVPEVLQVFTDKLIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
LESFDKDAIKAQFKATQKETGHRGKKLFMPIRVATTGQMHGPELPFAIELLGRDIILNRL
HHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
DKLLKEMGA
HHHHHHHCC
>Mature Secondary Structure 
SKEVRVRYAPSPTGHLHIGNARTALFNYLYAKHFDGKFIIRTEDTDDKRNVEGGEESQL
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHH
KYLKWLGLQWDEGADIGGEYGPYRQTERLDIYQEYLNELFAKNLAYKCYMTEDELEAERE
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHH
EQRQNGQVPKYSGAHRDLTPEQIEQFEAEGRQPSIRFRVPENTTYTFNDLVRGKITFESS
HHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCEEHHHHEEEEEEECCC
DFGDWVIVKKNGIPTYNFAVAIDDHLMKISDVLRGEEHISNTPKQMMIFDAFGWEAPRYG
CCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCC
HMTLILNEERKKLSKRDEHILQFIEQYRNLGYLPEAMFNFISLLGWSPVGEEEMFTQAQL
CEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IEIFDPERLSTSAAIFDQHKLKWMNNEYIKAADLDRVIDLALPHLIKAGKLSEDMDDETR
HHHCCCCHHCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
QWAENVISLYREQLRYGAEIVELTELFFQKEISYDEEAKEVLNGEQVPEVLQVFTDKLIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
LESFDKDAIKAQFKATQKETGHRGKKLFMPIRVATTGQMHGPELPFAIELLGRDIILNRL
HHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
DKLLKEMGA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376