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The map label for this gene is lysS

Identifier: 23097543

GI number: 23097543

Start: 89569

End: 91050

Strand: Direct

Name: lysS

Synonym: OB0088

Alternate gene names: 23097543

Gene position: 89569-91050 (Clockwise)

Preceding gene: 28563899

Following gene: 23097544

Centisome position: 2.47

GC content: 37.11

Gene sequence:

>1482_bases
ATGTCAGAAGAGTTAAATGAACATATGCAGGTTCGAAGAGATAAATTGGCAGAACACATGGAAAAAGGGTTGGATCCATT
TGGTGGTAAATTCGAACGTAGCCATCAGGCTACAGATCTTATTGAAAAATATGATTCCTATTCAAAAGAAGAATTAGAGG
AAACTACAGATGAAGTTACCATTGCTGGACGATTAATGACAAAGCGTGGAAAAGGAAAAGCTGGATTTGCTCATATTCAA
GATTTAAGTGGACAAATTCAATTATATGTACGAAAAGATATGATCGGTGACGATGCTTATGAAGTGTTTAAGTCGGCAGA
TTTAGGAGATATCGTCGGCGTTACTGGTGTTATGTTTAAAACAAATGTAGGAGAAATATCTGTTAAAGCAAAACAATTTC
AACTATTAACGAAATCCTTACGACCTTTACCAGAAAAGTATCATGGATTAAAGGATATTGAACAGCGTTATCGTCAACGT
TATCTGGACTTAATTACAAATCCTGATAGTAGAGGAACTTTTGTATCTCGTAGTAAAATCATTCAGTCGATGAGAGAGTA
TTTAAACGGACAAGGCTTTTTAGAAGTTGAAACACCGATGATGCATAGTATTCCTGGTGGAGCTTCTGCCCGTCCATTTA
TTACACATCATAACGCTTTAGATATAGAATTGTATATGCGAATAGCTATTGAATTACACCTAAAACGTTTAATGGTTGGC
GGACTAGAAAAAGTGTATGAAATAGGGCGCGTTTTCCGAAATGAAGGAGTATCTACAAGGCACAACCCTGAATTCACGAT
GATTGAGCTATATGAAGCGTACGCTGATTATCATGACATTATGGAACTTACAGAGAATCTCGTAGCTCATATTGCAAAAC
AAGTCCATGGTTCAACAACAATTACTTATGGAGAACATGAAATTAATCTAGAGCCAAAATGGACAAGACTGCATATCGTA
GATGCTGTTAAAGATGCTACAGGTGTGGATTTTTGGAAAGAAGTTTCAGATGAGGAAGCTCGAGCACTTGCTAAAGAGCA
TGGTGTGCAAGTAACTGAATCTATGAGTTATGGACATGTGGTTAATGAATTTTTTGAACAAAAAGTAGAAGAGACTCTTA
TTCAACCTACATTTATACACGGACATCCAGTGGAAATTTCTCCACTTGCAAAGAAAAATAAAGAAGATGAGCGATTCACA
GATCGTTTTGAGTTGTTTATTGTAGGAAGAGAACATGCGAATGCGTTTAGTGAATTGAATGATCCAATTGACCAACGCGC
TCGTTTTGAGGCTCAAGTAAAAGAAAGAGCAGAAGGAAACGATGAAGCACATTATATGGATGAGGATTTCTTAGAAGCAC
TTGAATATGGAATGCCACCAACTGGAGGACTCGGTATTGGTGTTGACCGCTTAGTAATGTTATTAACAAACTCACCATCC
ATTCGTGACGTATTACTTTTCCCGCAAATGCGTACAAAGTAA

Upstream 100 bases:

>100_bases
GTTATAAGGGAATTAGAGATCCTTTTTAAATAATGAATAATCAATTCAAGTCATTGTAAGATACGTTATACTTAGTTTAT
AGAGAATTGGAGTGATAAAA

Downstream 100 bases:

>100_bases
CTGGCGGGTTGAATCCCTCCTTATCAAAACAAGGATTTGATAAGGGGGGATTCTTTGTTTAAAACAGGATTATATTGGGT
ATATATAAAGTATGTATTCT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSEELNEHMQVRRDKLAEHMEKGLDPFGGKFERSHQATDLIEKYDSYSKEELEETTDEVTIAGRLMTKRGKGKAGFAHIQ
DLSGQIQLYVRKDMIGDDAYEVFKSADLGDIVGVTGVMFKTNVGEISVKAKQFQLLTKSLRPLPEKYHGLKDIEQRYRQR
YLDLITNPDSRGTFVSRSKIIQSMREYLNGQGFLEVETPMMHSIPGGASARPFITHHNALDIELYMRIAIELHLKRLMVG
GLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMELTENLVAHIAKQVHGSTTITYGEHEINLEPKWTRLHIV
DAVKDATGVDFWKEVSDEEARALAKEHGVQVTESMSYGHVVNEFFEQKVEETLIQPTFIHGHPVEISPLAKKNKEDERFT
DRFELFIVGREHANAFSELNDPIDQRARFEAQVKERAEGNDEAHYMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSPS
IRDVLLFPQMRTK

Sequences:

>Translated_493_residues
MSEELNEHMQVRRDKLAEHMEKGLDPFGGKFERSHQATDLIEKYDSYSKEELEETTDEVTIAGRLMTKRGKGKAGFAHIQ
DLSGQIQLYVRKDMIGDDAYEVFKSADLGDIVGVTGVMFKTNVGEISVKAKQFQLLTKSLRPLPEKYHGLKDIEQRYRQR
YLDLITNPDSRGTFVSRSKIIQSMREYLNGQGFLEVETPMMHSIPGGASARPFITHHNALDIELYMRIAIELHLKRLMVG
GLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMELTENLVAHIAKQVHGSTTITYGEHEINLEPKWTRLHIV
DAVKDATGVDFWKEVSDEEARALAKEHGVQVTESMSYGHVVNEFFEQKVEETLIQPTFIHGHPVEISPLAKKNKEDERFT
DRFELFIVGREHANAFSELNDPIDQRARFEAQVKERAEGNDEAHYMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSPS
IRDVLLFPQMRTK
>Mature_492_residues
SEELNEHMQVRRDKLAEHMEKGLDPFGGKFERSHQATDLIEKYDSYSKEELEETTDEVTIAGRLMTKRGKGKAGFAHIQD
LSGQIQLYVRKDMIGDDAYEVFKSADLGDIVGVTGVMFKTNVGEISVKAKQFQLLTKSLRPLPEKYHGLKDIEQRYRQRY
LDLITNPDSRGTFVSRSKIIQSMREYLNGQGFLEVETPMMHSIPGGASARPFITHHNALDIELYMRIAIELHLKRLMVGG
LEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMELTENLVAHIAKQVHGSTTITYGEHEINLEPKWTRLHIVD
AVKDATGVDFWKEVSDEEARALAKEHGVQVTESMSYGHVVNEFFEQKVEETLIQPTFIHGHPVEISPLAKKNKEDERFTD
RFELFIVGREHANAFSELNDPIDQRARFEAQVKERAEGNDEAHYMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSPSI
RDVLLFPQMRTK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=509, Percent_Identity=42.8290766208251, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI194272210, Length=509, Percent_Identity=42.8290766208251, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI40789249, Length=246, Percent_Identity=28.8617886178862, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI45439306, Length=474, Percent_Identity=23.4177215189873, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=53.1187122736419, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1789256, Length=495, Percent_Identity=52.3232323232323, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI87082379, Length=331, Percent_Identity=30.5135951661631, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788173, Length=272, Percent_Identity=26.1029411764706, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=502, Percent_Identity=43.8247011952191, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17535927, Length=502, Percent_Identity=43.8247011952191, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71994340, Length=486, Percent_Identity=44.8559670781893, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17551876, Length=474, Percent_Identity=22.7848101265823, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=250, Percent_Identity=23.6, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=492, Percent_Identity=43.9024390243902, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324256, Length=476, Percent_Identity=32.3529411764706, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6323011, Length=475, Percent_Identity=24.8421052631579, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321807, Length=473, Percent_Identity=24.9471458773784, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640849, Length=503, Percent_Identity=41.7495029821074, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24640851, Length=503, Percent_Identity=41.7495029821074, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24584738, Length=246, Percent_Identity=28.8617886178862, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_OCEIH (Q8EU10)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691009.1
- ProteinModelPortal:   Q8EU10
- SMR:   Q8EU10
- GeneID:   1015157
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0088
- NMPDR:   fig|221109.1.peg.89
- HOGENOM:   HBG631383
- OMA:   VDMIANP
- ProtClustDB:   PRK00484
- BioCyc:   OIHE221109:OB0088-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56437; Mature: 56306

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEELNEHMQVRRDKLAEHMEKGLDPFGGKFERSHQATDLIEKYDSYSKEELEETTDEVT
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEE
IAGRLMTKRGKGKAGFAHIQDLSGQIQLYVRKDMIGDDAYEVFKSADLGDIVGVTGVMFK
HHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHEEEE
TNVGEISVKAKQFQLLTKSLRPLPEKYHGLKDIEQRYRQRYLDLITNPDSRGTFVSRSKI
CCCCCEEEHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
IQSMREYLNGQGFLEVETPMMHSIPGGASARPFITHHNALDIELYMRIAIELHLKRLMVG
HHHHHHHHCCCCEEEECCCHHHCCCCCCCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHH
GLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMELTENLVAHIAKQVHGSTT
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ITYGEHEINLEPKWTRLHIVDAVKDATGVDFWKEVSDEEARALAKEHGVQVTESMSYGHV
EEECCEEECCCCCEEEEEEHHHHHCCCCCHHHHHCCHHHHHHHHHHCCCEEECCCCHHHH
VNEFFEQKVEETLIQPTFIHGHPVEISPLAKKNKEDERFTDRFELFIVGREHANAFSELN
HHHHHHHHHHHHHCCCEEECCCCCEECCHHHCCCCHHHHHCEEEEEEEECHHHHHHHHHC
DPIDQRARFEAQVKERAEGNDEAHYMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSPS
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
IRDVLLFPQMRTK
CCCEEECCCCCCC
>Mature Secondary Structure 
SEELNEHMQVRRDKLAEHMEKGLDPFGGKFERSHQATDLIEKYDSYSKEELEETTDEVT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEE
IAGRLMTKRGKGKAGFAHIQDLSGQIQLYVRKDMIGDDAYEVFKSADLGDIVGVTGVMFK
HHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHEEEE
TNVGEISVKAKQFQLLTKSLRPLPEKYHGLKDIEQRYRQRYLDLITNPDSRGTFVSRSKI
CCCCCEEEHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
IQSMREYLNGQGFLEVETPMMHSIPGGASARPFITHHNALDIELYMRIAIELHLKRLMVG
HHHHHHHHCCCCEEEECCCHHHCCCCCCCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHH
GLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMELTENLVAHIAKQVHGSTT
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ITYGEHEINLEPKWTRLHIVDAVKDATGVDFWKEVSDEEARALAKEHGVQVTESMSYGHV
EEECCEEECCCCCEEEEEEHHHHHCCCCCHHHHHCCHHHHHHHHHHCCCEEECCCCHHHH
VNEFFEQKVEETLIQPTFIHGHPVEISPLAKKNKEDERFTDRFELFIVGREHANAFSELN
HHHHHHHHHHHHHCCCEEECCCCCEECCHHHCCCCHHHHHCEEEEEEEECHHHHHHHHHC
DPIDQRARFEAQVKERAEGNDEAHYMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSPS
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
IRDVLLFPQMRTK
CCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376