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The map label for this gene is yhcA [H]

Identifier: 23097527

GI number: 23097527

Start: 70698

End: 72227

Strand: Reverse

Name: yhcA [H]

Synonym: OB0072

Alternate gene names: 23097527

Gene position: 72227-70698 (Counterclockwise)

Preceding gene: 23097528

Following gene: 23097500

Centisome position: 1.99

GC content: 37.91

Gene sequence:

>1530_bases
ATGAAGGATAGATCCACGTACCTTGATGCAACCAACTTACCAAAGGTACCAATTATATTTGTAATGGTAATGGGATCATT
TATCGCTATTCTGAATCAAACATTATTAAATGTAGCATTACCTGTCATGATTGTTGATATGAATATGTCTGCCAATCAAG
CACAGTCATTAACGACGATCTTTATGCTTGTGAATGGAATTCTTATTCCAATTACGGCTTTTTTAATGGAGAAGTTTACC
ACAAGACAATTGTTTCTAACAGCAATGTCACTATTTGCCCTAGGTACGCTAGTATGTGGATTAGCCTTTGGTTACCCATC
CCTCTTAGTTGGTCGTGTCATTCAAGCGGCTGGTGCAGGTATAATGATGCCACTGTTGATGAATGTAATTTTAAGTCTAT
TCCCTATCAATAAACGAGGATCTGCGATGGGATATATAGGACTTGCGATGATGTTTGCGCCAGCAATTGGGCCCACATTG
TCGGGATTGGTGGTCCAAAATTATTCATGGCGTGTATTATTCTTTATCGTTCTTCCTATTGCCATCATTGATATCATTTT
GGCCTTTTTCTTTTTACGTAACGTTACCAAACAATCCAATCCAAAAGTAAATTATATTTCGATATTAATGTCCACTGTCG
CATTTGGTGGATTACTCCTTGGATTTAGCCTAGCAGGAGAAATGGGCTGGAAACATGTAGAAGTTATTGCTCCATTAATG
ATAGGAGCAATTGTAATGGTGTTCTTTATACGGATGCAGTTGAAACTAAAAAATCCCATACTCGAATTTAGAGTGTTCAA
GTTTAGTATGTTCTCCCTAACAACGGTTATTAGTGTGGTCATAACCATGTCGATGTTTGCAGCAATGATTCTCGTGCCAA
TTTATTTACAAAACTTACGAGGTTTTTCTCCGTTAGCATCAGGATTGATGCTATTACCTGGGGCGTTAATCTCAGCATTT
ATGTCACCAATCACTGGTAAATTATTTGATAGAATTGGTGCACGACCGTTAGCAATTACTGGACTAGGGATAACCGCCAT
TACTACATTTCTGTTCTCTGATTTATCGAATGAAACTGGATATTATTATTTAATGTTTGTCTACAGCCTGCGCATGGTGG
GTATTTCAATGATTATGATGCCAATAATGACTGCTGGATTAAATCAATTGCCCCAGCGTCTATATTCACATGGAACCGCA
ATGGCTAATACGCTGCGACAAATGTCCGGTGCGATTGGAACTGCTTTTTTAGTTACCGTTATGAGTAGCCAAACGGTCAA
GCATATGGACGAAATGACACAAACAGTTGTATCGGAAGAACAGTTAAAAGTAATAGAGAACTTAGCAACAATTGAAGGAA
TTAATGATTCGTTTATGATTGCGACGCTTTTCGCTATTTTTGGATTTATACTATCCTTCTTCATAAAAAGAAGGTACCCA
CCCGAAGAATCAGTAGAAACTGTATCTAAAGAACAAAAGCGTAAGCAACCATTTAGAAACGTAGCGAGTGGAACGAATCA
ACTGAGATAA

Upstream 100 bases:

>100_bases
CTTCATGAGTTGTCTGAAGTTAGTGACGAAGATACCGAAAGCTTATTTCGCCTCCATCAGTTGATAATAGAAAAATTGAC
AACGGAGAGGAGTGATCGTT

Downstream 100 bases:

>100_bases
AGGAATCACGGTGAGGTACGAGCCGATGATGACTTTCACCCATTGGCATAAGTGCGACTAAGCCTCATAAAGAACATTTT
GCAGTCTTCTTTATCGTAGG

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKDRSTYLDATNLPKVPIIFVMVMGSFIAILNQTLLNVALPVMIVDMNMSANQAQSLTTIFMLVNGILIPITAFLMEKFT
TRQLFLTAMSLFALGTLVCGLAFGYPSLLVGRVIQAAGAGIMMPLLMNVILSLFPINKRGSAMGYIGLAMMFAPAIGPTL
SGLVVQNYSWRVLFFIVLPIAIIDIILAFFFLRNVTKQSNPKVNYISILMSTVAFGGLLLGFSLAGEMGWKHVEVIAPLM
IGAIVMVFFIRMQLKLKNPILEFRVFKFSMFSLTTVISVVITMSMFAAMILVPIYLQNLRGFSPLASGLMLLPGALISAF
MSPITGKLFDRIGARPLAITGLGITAITTFLFSDLSNETGYYYLMFVYSLRMVGISMIMMPIMTAGLNQLPQRLYSHGTA
MANTLRQMSGAIGTAFLVTVMSSQTVKHMDEMTQTVVSEEQLKVIENLATIEGINDSFMIATLFAIFGFILSFFIKRRYP
PEESVETVSKEQKRKQPFRNVASGTNQLR

Sequences:

>Translated_509_residues
MKDRSTYLDATNLPKVPIIFVMVMGSFIAILNQTLLNVALPVMIVDMNMSANQAQSLTTIFMLVNGILIPITAFLMEKFT
TRQLFLTAMSLFALGTLVCGLAFGYPSLLVGRVIQAAGAGIMMPLLMNVILSLFPINKRGSAMGYIGLAMMFAPAIGPTL
SGLVVQNYSWRVLFFIVLPIAIIDIILAFFFLRNVTKQSNPKVNYISILMSTVAFGGLLLGFSLAGEMGWKHVEVIAPLM
IGAIVMVFFIRMQLKLKNPILEFRVFKFSMFSLTTVISVVITMSMFAAMILVPIYLQNLRGFSPLASGLMLLPGALISAF
MSPITGKLFDRIGARPLAITGLGITAITTFLFSDLSNETGYYYLMFVYSLRMVGISMIMMPIMTAGLNQLPQRLYSHGTA
MANTLRQMSGAIGTAFLVTVMSSQTVKHMDEMTQTVVSEEQLKVIENLATIEGINDSFMIATLFAIFGFILSFFIKRRYP
PEESVETVSKEQKRKQPFRNVASGTNQLR
>Mature_509_residues
MKDRSTYLDATNLPKVPIIFVMVMGSFIAILNQTLLNVALPVMIVDMNMSANQAQSLTTIFMLVNGILIPITAFLMEKFT
TRQLFLTAMSLFALGTLVCGLAFGYPSLLVGRVIQAAGAGIMMPLLMNVILSLFPINKRGSAMGYIGLAMMFAPAIGPTL
SGLVVQNYSWRVLFFIVLPIAIIDIILAFFFLRNVTKQSNPKVNYISILMSTVAFGGLLLGFSLAGEMGWKHVEVIAPLM
IGAIVMVFFIRMQLKLKNPILEFRVFKFSMFSLTTVISVVITMSMFAAMILVPIYLQNLRGFSPLASGLMLLPGALISAF
MSPITGKLFDRIGARPLAITGLGITAITTFLFSDLSNETGYYYLMFVYSLRMVGISMIMMPIMTAGLNQLPQRLYSHGTA
MANTLRQMSGAIGTAFLVTVMSSQTVKHMDEMTQTVVSEEQLKVIENLATIEGINDSFMIATLFAIFGFILSFFIKRRYP
PEESVETVSKEQKRKQPFRNVASGTNQLR

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=417, Percent_Identity=25.8992805755396, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1788710, Length=428, Percent_Identity=25.4672897196262, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081983, Length=421, Percent_Identity=29.4536817102138, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790195, Length=429, Percent_Identity=27.2727272727273, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788392, Length=432, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323735, Length=323, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56149; Mature: 56149

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
7.5 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
7.5 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKDRSTYLDATNLPKVPIIFVMVMGSFIAILNQTLLNVALPVMIVDMNMSANQAQSLTTI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHH
FMLVNGILIPITAFLMEKFTTRQLFLTAMSLFALGTLVCGLAFGYPSLLVGRVIQAAGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCH
IMMPLLMNVILSLFPINKRGSAMGYIGLAMMFAPAIGPTLSGLVVQNYSWRVLFFIVLPI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHH
AIIDIILAFFFLRNVTKQSNPKVNYISILMSTVAFGGLLLGFSLAGEMGWKHVEVIAPLM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IGAIVMVFFIRMQLKLKNPILEFRVFKFSMFSLTTVISVVITMSMFAAMILVPIYLQNLR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GFSPLASGLMLLPGALISAFMSPITGKLFDRIGARPLAITGLGITAITTFLFSDLSNETG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCC
YYYLMFVYSLRMVGISMIMMPIMTAGLNQLPQRLYSHGTAMANTLRQMSGAIGTAFLVTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MSSQTVKHMDEMTQTVVSEEQLKVIENLATIEGINDSFMIATLFAIFGFILSFFIKRRYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PEESVETVSKEQKRKQPFRNVASGTNQLR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]