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The map label for this gene is ipk

Identifier: 23097510

GI number: 23097510

Start: 53741

End: 54583

Strand: Direct

Name: ipk

Synonym: OB0055

Alternate gene names: 23097510

Gene position: 53741-54583 (Clockwise)

Preceding gene: 23097509

Following gene: 23097511

Centisome position: 1.48

GC content: 39.15

Gene sequence:

>843_bases
ATGGAGAAAGCACCTGCAAAGATTAATCTGTCTTTAGATGTTTTGCATAAGCGAGATGATGGTTATCATGATGTTGAAAT
GGTGATGACAACCATTGATTTATCTGACCGAATCGAATTACAACAACTTCCTAAAGATGAGATACAAATTGCATTGGAAA
GCAGATATGTTCCCAGTGATGAACGGAATTTAGCTTACCAAGCAGCAAAATTATTTAAGAAGACATATCAAATTCCAAAT
GGAGTTCGTATCCAAATAGAGAAAAACATCCCTGTATCTGCAGGTCTTGCTGGGGGTAGTACAGATGCTGCAGCTGTTTT
ACGAGGATTAAATCGCTTATTTCATGTAAATGCGCCTCTGAAAGATTTAGCAAAATTAGGTTCTAATCTAGGTGCAGATG
TTGCTTTTTGTGTATATGGAAAGACAGCCATTGCAAAGGGCTTCGGAGATCAAATTGAAATGCTTCCCTCACCACCAAAC
TGTTGGGTAATTACTGCAAGACCAGATATTGGAGTGTCCACTAGAACGATCTTTGAACGGATTGAACTACAACACCTTCA
ACACCCGGATACAAAGAAAGTATTAGAGGCACTACAAAAAAGAGATTTTTATCAAATTTGTATAAGTTTAGGAAATGCTT
TGGAAGATATAACATTTGCACTTCATCCGGAAGTTAATCGAATTAAGAATGCCATGCTGCAAGCTGGTGCCGACGGAGCA
ATGATGAGTGGTAGCGGGCCAACAGTATTCGGTCTGGTACAACACTTTAGTAAAGCGCAACGGATTTATAATAGTATGCG
CGGTTTTTGTGAGGATGTACAGTTGGTCCGGATTTTAGGATGA

Upstream 100 bases:

>100_bases
TTTTTATATTTATTCGCCTCTATATCTTGAATATGATACAATAATCTATATTTTTAAGCCCGTGTATAAAGAAAGAAAGG
TAGGTGATCTGGTGATTGTC

Downstream 100 bases:

>100_bases
GTATTGTTTAAAGTCGTATATTAATGGTATATTATATATAAAACATTCGGGTTTGGAGGTGTCACAAATGAAAAGAAGTA
ATCGATTGGTAGTTTTAACA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MEKAPAKINLSLDVLHKRDDGYHDVEMVMTTIDLSDRIELQQLPKDEIQIALESRYVPSDERNLAYQAAKLFKKTYQIPN
GVRIQIEKNIPVSAGLAGGSTDAAAVLRGLNRLFHVNAPLKDLAKLGSNLGADVAFCVYGKTAIAKGFGDQIEMLPSPPN
CWVITARPDIGVSTRTIFERIELQHLQHPDTKKVLEALQKRDFYQICISLGNALEDITFALHPEVNRIKNAMLQAGADGA
MMSGSGPTVFGLVQHFSKAQRIYNSMRGFCEDVQLVRILG

Sequences:

>Translated_280_residues
MEKAPAKINLSLDVLHKRDDGYHDVEMVMTTIDLSDRIELQQLPKDEIQIALESRYVPSDERNLAYQAAKLFKKTYQIPN
GVRIQIEKNIPVSAGLAGGSTDAAAVLRGLNRLFHVNAPLKDLAKLGSNLGADVAFCVYGKTAIAKGFGDQIEMLPSPPN
CWVITARPDIGVSTRTIFERIELQHLQHPDTKKVLEALQKRDFYQICISLGNALEDITFALHPEVNRIKNAMLQAGADGA
MMSGSGPTVFGLVQHFSKAQRIYNSMRGFCEDVQLVRILG
>Mature_280_residues
MEKAPAKINLSLDVLHKRDDGYHDVEMVMTTIDLSDRIELQQLPKDEIQIALESRYVPSDERNLAYQAAKLFKKTYQIPN
GVRIQIEKNIPVSAGLAGGSTDAAAVLRGLNRLFHVNAPLKDLAKLGSNLGADVAFCVYGKTAIAKGFGDQIEMLPSPPN
CWVITARPDIGVSTRTIFERIELQHLQHPDTKKVLEALQKRDFYQICISLGNALEDITFALHPEVNRIKNAMLQAGADGA
MMSGSGPTVFGLVQHFSKAQRIYNSMRGFCEDVQLVRILG

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=265, Percent_Identity=31.6981132075472, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_OCEIH (Q8EU37)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690976.1
- ProteinModelPortal:   Q8EU37
- SMR:   Q8EU37
- GeneID:   1015116
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0055
- NMPDR:   fig|221109.1.peg.55
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK00128
- BioCyc:   OIHE221109:OB0055-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30958; Mature: 30958

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: ACT_SITE 7-7 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKAPAKINLSLDVLHKRDDGYHDVEMVMTTIDLSDRIELQQLPKDEIQIALESRYVPSD
CCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCHHHHEEHHHCCCCCCC
ERNLAYQAAKLFKKTYQIPNGVRIQIEKNIPVSAGLAGGSTDAAAVLRGLNRLFHVNAPL
CHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCH
KDLAKLGSNLGADVAFCVYGKTAIAKGFGDQIEMLPSPPNCWVITARPDIGVSTRTIFER
HHHHHHCCCCCCCEEEEEECHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHH
IELQHLQHPDTKKVLEALQKRDFYQICISLGNALEDITFALHPEVNRIKNAMLQAGADGA
HHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHCCCCCC
MMSGSGPTVFGLVQHFSKAQRIYNSMRGFCEDVQLVRILG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKAPAKINLSLDVLHKRDDGYHDVEMVMTTIDLSDRIELQQLPKDEIQIALESRYVPSD
CCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCHHHHEEHHHCCCCCCC
ERNLAYQAAKLFKKTYQIPNGVRIQIEKNIPVSAGLAGGSTDAAAVLRGLNRLFHVNAPL
CHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCH
KDLAKLGSNLGADVAFCVYGKTAIAKGFGDQIEMLPSPPNCWVITARPDIGVSTRTIFER
HHHHHHCCCCCCCEEEEEECHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHH
IELQHLQHPDTKKVLEALQKRDFYQICISLGNALEDITFALHPEVNRIKNAMLQAGADGA
HHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHCCCCCC
MMSGSGPTVFGLVQHFSKAQRIYNSMRGFCEDVQLVRILG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376