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The map label for this gene is metG

Identifier: 23097501

GI number: 23097501

Start: 45421

End: 47442

Strand: Direct

Name: metG

Synonym: OB0046

Alternate gene names: 23097501

Gene position: 45421-47442 (Clockwise)

Preceding gene: 23097499

Following gene: 23097502

Centisome position: 1.25

GC content: 38.18

Gene sequence:

>2022_bases
ATGGCAAGAGAAAATGCATTTTATATAACCACGCCAATATACTATCCTAGTGGAAAATTACATATTGGAAATGCGTACAC
GACAATCGCTTGTGATGTAATGGCACGTTATAAACGCATGCGTGGATTCGATGTTTTTTATTTAACAGGTAGTGATGAGC
ATGGACAAAAAATCGAACAAAAAGCGAAAGAGATGAATATTTCTCCGAAAGCCTATGTAGATGATATGGCTGAAGGAATG
AAAAAGTTATGGAATACATTAGAAATTAGTAATGATAAGTTTATTCGCACAACGGAAGAACAACATAAAAAAGTAGTAGC
CGATATATTTGAACGATTCTTAGAACAAGGTGATATTTACTTAGATGAATATGAAGGATGGTATTCGGTTCCTGATGAAA
CATTCTATACTGAAACACAGTTAGAGGATGTAGAAAGAGATGACGACGGTAATGTTATTGGTGGTAAATCTCCAGATAGT
GGACATCCAGTCGAATTAATTAAAGAAGAATCCTATTTCTTCCGGATGAGTAAATATGCCGATCGTTTGTTGAAATTTTA
TGAAGACAACCCGGAATTTATTCAACCAGAATCTCGAAAAAATGAAATGATTAATAACTTTATTAAACCAGGTTTAGAGG
ACCTAGCCGTTTCTCGTACAACATTCAGCTGGGGTGTACAAGTTCCGAGTAACCCAAAACACGTTGTATACGTCTGGATT
GATGCGCTTACCAACTATATCACAGCATTAGGTTATGGCTCAGAAGACACATCCCTTTATGACAAGTTTTGGCCAGCGGA
TGTGCATATGGTTGGAAAAGAGATTGTACGATTCCATACGATTTATTGGCCGATTATGCTAATGGCTCTAGACTTACCTT
TACCAAAAAAAGTCTTTGCACATGGCTGGCTTTTAATGAAGGATGGAAAAATGTCGAAATCAAAAGGGAATGTCGTATAT
CCTGAAATGCTTGTCGAACGTTATGGTCTAGATGCTTTACGTTACTACCTCATGCGTGAAGTTGCCTTTGGAAGCGATGG
CGTCTTTACACCGGAAGATTTTATTTCTCGTGTAAATTATGACCTAGCAAATGACCTCGGAAACTTATTAAATCGGACAG
TGGCGATGATTAATAAGTATTTTGATGGAAAGGTTCCTGAATTTAAAGGGGAAGTAACAAGTTTTGATGGCGAGTTACAA
ACAACCGCAAATAATGCAGTCAAAGAATATGAGAAACATATGGAAGGCATGCAATTTAGCGATGCATTAAAACAAGTGTG
GATTTTAATTTCGAGAGCAAACAAATATATTGATGAAACAGAACCTTGGATAGTAGCAAAAGATGAAGGTCGCAGAAATG
AATTAGCAAGTGTCATGGTTCATTTAGCGGAAAGTTTACATGCAGCCGCGCTAATGTTACAACCATTCCTAACTCATGCA
CCGAAGAAAATCGCAGAGCAACTTGGTCTAGGAGAAGAATATGGGCTAGATTGGGGTACAATCGGTTTTGGCAATTTCCC
AGAAAATACAACCGTGGTTAAGAAAGGCACACCAATCTTCCCTCGTCTTGATTTGGATGAAGAAGCTGCTTATATTCGTG
ATCAAATGGCGAATGGTGCAAACGCAGCTTCTTCAGAAGATGAGACGGGCGACTGGGACCCTAATGAGACAGACCTTGTC
TCTGAAAAAGAAAAACAGATTAAATATGATGTGTTTGATAAGGTTGAACTAAAAGTAGCTGAAGTGAAAGACTGTAGTAA
AGTAGAGGGCGCAGATAAACTATTAAAATTCCGATTAGATGCTGGAGATAATGGAGATCGACAAATACTTTCCGGCATCG
CTGAATATTATTCGGAACCAGAGCAATTAATTGGCAAAAAAGTTGTCATCGTCGCTAATTTGAAGCCACGTAAAATGCGT
GGAGAAATTAGTCAAGGTATGATATTATCAGCGGAATACGACGGCAAACTACAAATTGTAGAAGCACCAAGTGAAGCGCC
TAACGGTTCGTCAATTTCTTAA

Upstream 100 bases:

>100_bases
TAAGTAAAGCGCGGTAGATGTACACGATTTGGCAAATTGCGTTATAATAGCTACAATAGAATCTATATATAATAAGGAAG
AGATTTAGGAGGCACAACGA

Downstream 100 bases:

>100_bases
TCGAAATAAGAAATGAAGTAAAAACTATAAGCTAAATCGGATCGAGCTATAATGGGGTGACACTTCCTAGATACCCGGAT
TATAGCTCGGTTTTTTATTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MARENAFYITTPIYYPSGKLHIGNAYTTIACDVMARYKRMRGFDVFYLTGSDEHGQKIEQKAKEMNISPKAYVDDMAEGM
KKLWNTLEISNDKFIRTTEEQHKKVVADIFERFLEQGDIYLDEYEGWYSVPDETFYTETQLEDVERDDDGNVIGGKSPDS
GHPVELIKEESYFFRMSKYADRLLKFYEDNPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFSWGVQVPSNPKHVVYVWI
DALTNYITALGYGSEDTSLYDKFWPADVHMVGKEIVRFHTIYWPIMLMALDLPLPKKVFAHGWLLMKDGKMSKSKGNVVY
PEMLVERYGLDALRYYLMREVAFGSDGVFTPEDFISRVNYDLANDLGNLLNRTVAMINKYFDGKVPEFKGEVTSFDGELQ
TTANNAVKEYEKHMEGMQFSDALKQVWILISRANKYIDETEPWIVAKDEGRRNELASVMVHLAESLHAAALMLQPFLTHA
PKKIAEQLGLGEEYGLDWGTIGFGNFPENTTVVKKGTPIFPRLDLDEEAAYIRDQMANGANAASSEDETGDWDPNETDLV
SEKEKQIKYDVFDKVELKVAEVKDCSKVEGADKLLKFRLDAGDNGDRQILSGIAEYYSEPEQLIGKKVVIVANLKPRKMR
GEISQGMILSAEYDGKLQIVEAPSEAPNGSSIS

Sequences:

>Translated_673_residues
MARENAFYITTPIYYPSGKLHIGNAYTTIACDVMARYKRMRGFDVFYLTGSDEHGQKIEQKAKEMNISPKAYVDDMAEGM
KKLWNTLEISNDKFIRTTEEQHKKVVADIFERFLEQGDIYLDEYEGWYSVPDETFYTETQLEDVERDDDGNVIGGKSPDS
GHPVELIKEESYFFRMSKYADRLLKFYEDNPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFSWGVQVPSNPKHVVYVWI
DALTNYITALGYGSEDTSLYDKFWPADVHMVGKEIVRFHTIYWPIMLMALDLPLPKKVFAHGWLLMKDGKMSKSKGNVVY
PEMLVERYGLDALRYYLMREVAFGSDGVFTPEDFISRVNYDLANDLGNLLNRTVAMINKYFDGKVPEFKGEVTSFDGELQ
TTANNAVKEYEKHMEGMQFSDALKQVWILISRANKYIDETEPWIVAKDEGRRNELASVMVHLAESLHAAALMLQPFLTHA
PKKIAEQLGLGEEYGLDWGTIGFGNFPENTTVVKKGTPIFPRLDLDEEAAYIRDQMANGANAASSEDETGDWDPNETDLV
SEKEKQIKYDVFDKVELKVAEVKDCSKVEGADKLLKFRLDAGDNGDRQILSGIAEYYSEPEQLIGKKVVIVANLKPRKMR
GEISQGMILSAEYDGKLQIVEAPSEAPNGSSIS
>Mature_672_residues
ARENAFYITTPIYYPSGKLHIGNAYTTIACDVMARYKRMRGFDVFYLTGSDEHGQKIEQKAKEMNISPKAYVDDMAEGMK
KLWNTLEISNDKFIRTTEEQHKKVVADIFERFLEQGDIYLDEYEGWYSVPDETFYTETQLEDVERDDDGNVIGGKSPDSG
HPVELIKEESYFFRMSKYADRLLKFYEDNPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFSWGVQVPSNPKHVVYVWID
ALTNYITALGYGSEDTSLYDKFWPADVHMVGKEIVRFHTIYWPIMLMALDLPLPKKVFAHGWLLMKDGKMSKSKGNVVYP
EMLVERYGLDALRYYLMREVAFGSDGVFTPEDFISRVNYDLANDLGNLLNRTVAMINKYFDGKVPEFKGEVTSFDGELQT
TANNAVKEYEKHMEGMQFSDALKQVWILISRANKYIDETEPWIVAKDEGRRNELASVMVHLAESLHAAALMLQPFLTHAP
KKIAEQLGLGEEYGLDWGTIGFGNFPENTTVVKKGTPIFPRLDLDEEAAYIRDQMANGANAASSEDETGDWDPNETDLVS
EKEKQIKYDVFDKVELKVAEVKDCSKVEGADKLLKFRLDAGDNGDRQILSGIAEYYSEPEQLIGKKVVIVANLKPRKMRG
EISQGMILSAEYDGKLQIVEAPSEAPNGSSIS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=546, Percent_Identity=31.8681318681319, Blast_Score=280, Evalue=4e-75,
Organism=Homo sapiens, GI14043022, Length=633, Percent_Identity=25.7503949447077, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI7661872, Length=189, Percent_Identity=28.042328042328, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788432, Length=686, Percent_Identity=29.1545189504373, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1789455, Length=90, Percent_Identity=38.8888888888889, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=500, Percent_Identity=32, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71992781, Length=384, Percent_Identity=31.25, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17540794, Length=604, Percent_Identity=24.5033112582781, Blast_Score=161, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71997510, Length=178, Percent_Identity=30.3370786516854, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71997517, Length=178, Percent_Identity=30.3370786516854, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321610, Length=527, Percent_Identity=29.9810246679317, Blast_Score=253, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6321704, Length=519, Percent_Identity=24.2774566473988, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323414, Length=226, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358483, Length=520, Percent_Identity=31.7307692307692, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24655311, Length=523, Percent_Identity=26.9598470363289, Blast_Score=155, Evalue=1e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_OCEIH (P59079)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690967.1
- ProteinModelPortal:   P59079
- GeneID:   1015098
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0046
- NMPDR:   fig|221109.1.peg.46
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK12267
- BioCyc:   OIHE221109:OB0046-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 76611; Mature: 76480

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD; PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARENAFYITTPIYYPSGKLHIGNAYTTIACDVMARYKRMRGFDVFYLTGSDEHGQKIEQ
CCCCCCEEEECCEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
KAKEMNISPKAYVDDMAEGMKKLWNTLEISNDKFIRTTEEQHKKVVADIFERFLEQGDIY
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCEE
LDEYEGWYSVPDETFYTETQLEDVERDDDGNVIGGKSPDSGHPVELIKEESYFFRMSKYA
EECCCCCCCCCCCCEECHHHHHHCCCCCCCCEECCCCCCCCCCHHHEECCHHHHHHHHHH
DRLLKFYEDNPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFSWGVQVPSNPKHVVYVWI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCEEEEECEECCCCCCEEEEEEH
DALTNYITALGYGSEDTSLYDKFWPADVHMVGKEIVRFHTIYWPIMLMALDLPLPKKVFA
HHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRYYLMREVAFGSDGVFTPEDFISRVNY
CCEEEEECCCCCCCCCCEECHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCH
DLANDLGNLLNRTVAMINKYFDGKVPEFKGEVTSFDGELQTTANNAVKEYEKHMEGMQFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
DALKQVWILISRANKYIDETEPWIVAKDEGRRNELASVMVHLAESLHAAALMLQPFLTHA
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKKIAEQLGLGEEYGLDWGTIGFGNFPENTTVVKKGTPIFPRLDLDEEAAYIRDQMANGA
HHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCC
NAASSEDETGDWDPNETDLVSEKEKQIKYDVFDKVELKVAEVKDCSKVEGADKLLKFRLD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHHCCHHHHHEEEEC
AGDNGDRQILSGIAEYYSEPEQLIGKKVVIVANLKPRKMRGEISQGMILSAEYDGKLQIV
CCCCCCHHHHHHHHHHHCCHHHHCCCEEEEEECCCCHHHHCCCCCCEEEEEECCCEEEEE
EAPSEAPNGSSIS
ECCCCCCCCCCCC
>Mature Secondary Structure 
ARENAFYITTPIYYPSGKLHIGNAYTTIACDVMARYKRMRGFDVFYLTGSDEHGQKIEQ
CCCCCEEEECCEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
KAKEMNISPKAYVDDMAEGMKKLWNTLEISNDKFIRTTEEQHKKVVADIFERFLEQGDIY
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCEE
LDEYEGWYSVPDETFYTETQLEDVERDDDGNVIGGKSPDSGHPVELIKEESYFFRMSKYA
EECCCCCCCCCCCCEECHHHHHHCCCCCCCCEECCCCCCCCCCHHHEECCHHHHHHHHHH
DRLLKFYEDNPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFSWGVQVPSNPKHVVYVWI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCEEEEECEECCCCCCEEEEEEH
DALTNYITALGYGSEDTSLYDKFWPADVHMVGKEIVRFHTIYWPIMLMALDLPLPKKVFA
HHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRYYLMREVAFGSDGVFTPEDFISRVNY
CCEEEEECCCCCCCCCCEECHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCH
DLANDLGNLLNRTVAMINKYFDGKVPEFKGEVTSFDGELQTTANNAVKEYEKHMEGMQFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
DALKQVWILISRANKYIDETEPWIVAKDEGRRNELASVMVHLAESLHAAALMLQPFLTHA
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKKIAEQLGLGEEYGLDWGTIGFGNFPENTTVVKKGTPIFPRLDLDEEAAYIRDQMANGA
HHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCC
NAASSEDETGDWDPNETDLVSEKEKQIKYDVFDKVELKVAEVKDCSKVEGADKLLKFRLD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHHCCHHHHHEEEEC
AGDNGDRQILSGIAEYYSEPEQLIGKKVVIVANLKPRKMRGEISQGMILSAEYDGKLQIV
CCCCCCHHHHHHHHHHHCCHHHHCCCEEEEEECCCCHHHHCCCCCCEEEEEECCCEEEEE
EAPSEAPNGSSIS
ECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376