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The map label for this gene is holB [H]

Identifier: 23097494

GI number: 23097494

Start: 40378

End: 41367

Strand: Direct

Name: holB [H]

Synonym: OB0039

Alternate gene names: 23097494

Gene position: 40378-41367 (Clockwise)

Preceding gene: 23097493

Following gene: 23097495

Centisome position: 1.11

GC content: 36.26

Gene sequence:

>990_bases
ATGATAACTTGGGAGGAAATCACAGACCTTCAGCCGATTGCTAGTCGAACAATCATCAATAGTATAAATAAAGGCAGGGT
ATCCCACGCTTACCTTCTACAAGGCGAACGAGGAACAGGGAAGCGGGACATTGCATTATTATTAGCAAAGTATTTATTCT
GTTTGCATCGTGAAGGAGCAAATCCTTGCCATACATGTAATAATTGTAAACGAATAGATTCGGGTAATCATCCTGACTTG
CATTGGATTGAACCAGATGGGCAATCGATTAAAATCGAACAGGTGCGTAATTTGCAAAAGGAATTCACATATTCAGGGAT
GGAATCGAATCAAAAAGTATATGTTATTCAGGATGCAGATACATTAACGGTAAATGCAGCAAATCGTTTGCTTAAATTTT
TGGAAGAACCAAGTAAGCAAACAACCGCAATTATGCTGACAGAGAATGGACACTCGATACTATCAACCATTCGTTCACGA
TGCCAAATTATAGAATTACAGCCTCTCCCACCTGATGTAATGAATCAAAGATTACAAGATGAAGGGGTTCACCACTTGGA
AGCAAACCTTTTAAGTGCCTTAACAAATAATACAAAAGAAGCTTTGTCATTACACCAAGATGAGTGGTTTGCAGGTGCAA
GAAAGATAGTGGTACAATTAATAGAAATGTATGTTCAAAAACCAGAGGATGTTTATTTATTTATCCACCAACAATGGATA
CCTCATTTTAAAGAACGTACCGAACAACAAATGGGATTGGATTTATTATTATTAGGATTTCAGGATATCCTTCATCAACA
TATTAAAGAAGACACTACAACAGTCTTATTACCTAAAGAAGATGCAAAAATGGAACGGGCAATGATGTCTTTTTCCAAAG
AGCAATTATTATTTATATTGCAGCTGATTTTAACAGCAAAACGAAAATTAAAGCAAAATGTTCAGCCGACGTTAGTAATG
GAACAATTAGCACTTCAAATACAGAGGTGA

Upstream 100 bases:

>100_bases
ATTAGGTCTAATTGGTGAGATCAAAGGTCTATTGATTAATATATATACATGAATAACAAGTGATCTCTATTAAGTGGGGA
TCAATTAGGATGGATGAATG

Downstream 100 bases:

>100_bases
AAAAATGATAGAAGTTATAGGAGTACGATTTAAGAAGGCGGGTAAAATCTATTATTTCGATCCAGACCAAGATAATATAG
AGTTAGATAGTTATGTCATT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MITWEEITDLQPIASRTIINSINKGRVSHAYLLQGERGTGKRDIALLLAKYLFCLHREGANPCHTCNNCKRIDSGNHPDL
HWIEPDGQSIKIEQVRNLQKEFTYSGMESNQKVYVIQDADTLTVNAANRLLKFLEEPSKQTTAIMLTENGHSILSTIRSR
CQIIELQPLPPDVMNQRLQDEGVHHLEANLLSALTNNTKEALSLHQDEWFAGARKIVVQLIEMYVQKPEDVYLFIHQQWI
PHFKERTEQQMGLDLLLLGFQDILHQHIKEDTTTVLLPKEDAKMERAMMSFSKEQLLFILQLILTAKRKLKQNVQPTLVM
EQLALQIQR

Sequences:

>Translated_329_residues
MITWEEITDLQPIASRTIINSINKGRVSHAYLLQGERGTGKRDIALLLAKYLFCLHREGANPCHTCNNCKRIDSGNHPDL
HWIEPDGQSIKIEQVRNLQKEFTYSGMESNQKVYVIQDADTLTVNAANRLLKFLEEPSKQTTAIMLTENGHSILSTIRSR
CQIIELQPLPPDVMNQRLQDEGVHHLEANLLSALTNNTKEALSLHQDEWFAGARKIVVQLIEMYVQKPEDVYLFIHQQWI
PHFKERTEQQMGLDLLLLGFQDILHQHIKEDTTTVLLPKEDAKMERAMMSFSKEQLLFILQLILTAKRKLKQNVQPTLVM
EQLALQIQR
>Mature_329_residues
MITWEEITDLQPIASRTIINSINKGRVSHAYLLQGERGTGKRDIALLLAKYLFCLHREGANPCHTCNNCKRIDSGNHPDL
HWIEPDGQSIKIEQVRNLQKEFTYSGMESNQKVYVIQDADTLTVNAANRLLKFLEEPSKQTTAIMLTENGHSILSTIRSR
CQIIELQPLPPDVMNQRLQDEGVHHLEANLLSALTNNTKEALSLHQDEWFAGARKIVVQLIEMYVQKPEDVYLFIHQQWI
PHFKERTEQQMGLDLLLLGFQDILHQHIKEDTTTVLLPKEDAKMERAMMSFSKEQLLFILQLILTAKRKLKQNVQPTLVM
EQLALQIQR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=215, Percent_Identity=34.4186046511628, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787341, Length=155, Percent_Identity=32.9032258064516, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37931; Mature: 37931

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITWEEITDLQPIASRTIINSINKGRVSHAYLLQGERGTGKRDIALLLAKYLFCLHREGA
CCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
NPCHTCNNCKRIDSGNHPDLHWIEPDGQSIKIEQVRNLQKEFTYSGMESNQKVYVIQDAD
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCC
TLTVNAANRLLKFLEEPSKQTTAIMLTENGHSILSTIRSRCQIIELQPLPPDVMNQRLQD
EEEEHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHH
EGVHHLEANLLSALTNNTKEALSLHQDEWFAGARKIVVQLIEMYVQKPEDVYLFIHQQWI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHC
PHFKERTEQQMGLDLLLLGFQDILHQHIKEDTTTVLLPKEDAKMERAMMSFSKEQLLFIL
HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
QLILTAKRKLKQNVQPTLVMEQLALQIQR
HHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MITWEEITDLQPIASRTIINSINKGRVSHAYLLQGERGTGKRDIALLLAKYLFCLHREGA
CCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
NPCHTCNNCKRIDSGNHPDLHWIEPDGQSIKIEQVRNLQKEFTYSGMESNQKVYVIQDAD
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCC
TLTVNAANRLLKFLEEPSKQTTAIMLTENGHSILSTIRSRCQIIELQPLPPDVMNQRLQD
EEEEHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHH
EGVHHLEANLLSALTNNTKEALSLHQDEWFAGARKIVVQLIEMYVQKPEDVYLFIHQQWI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHC
PHFKERTEQQMGLDLLLLGFQDILHQHIKEDTTTVLLPKEDAKMERAMMSFSKEQLLFIL
HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
QLILTAKRKLKQNVQPTLVMEQLALQIQR
HHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]