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The map label for this gene is yaaI [H]

Identifier: 23097480

GI number: 23097480

Start: 29612

End: 30145

Strand: Reverse

Name: yaaI [H]

Synonym: OB0025

Alternate gene names: 23097480

Gene position: 30145-29612 (Counterclockwise)

Preceding gene: 23097500

Following gene: 23097479

Centisome position: 0.83

GC content: 32.58

Gene sequence:

>534_bases
ATGAATTTTTCATCTGAAAATACAGCTGTAGTCTTTGTTGATTTAATCAATGACTTTCAATTTGATGGTGGAGATGACCT
ACTTCACCACACAAAACAAATCTTACCTAATGTTATAAAACTACGCTCCTTTGCTAAGGAAAACAATTTACCTATTATCT
ATGTTAATGATCATTACGGCTTGTGGCAAGCTGATTTTAACAAAGTAATTGATTATTGTACGAATGAAAAAAGTAAAGAA
ATAATCGATAAGATTAAACCCGATGAAGATGATTATTTCTTAATCAAGCCGCAGCATTCCGCCTTTTTCCAGACTCCTCT
TCATTCCTTACTTATAGAACTAGAGAGAAATCATGTAATACTTGCCGGAATAGCCGGAGATATTTGTATTTTGTTCAGTG
CAAAAGATGCCTACATGTACAAAATAGACATGTCTATACCAAAAAATTGTATGGCTTCTGAACAAGAAGAAAATAATAAC
TACGCTTTATATTTAATGGAATCCGTCATGGATGCAGATATATCTCCAATTTAG

Upstream 100 bases:

>100_bases
ACTGATAATTCGTCTTTCACAGTATCACATCTCTTTATTTAAAGTTTTAATATAGATTTGAACGGTAATAATAATAATAG
AATATCAAAGGAGGAGCTTT

Downstream 100 bases:

>100_bases
CATAATGCATTTACCCTCCTCATATAGATAGTTAGAGACTCTATGTAAGGAGGTAGTTCCTTTGCAAATTCACGTTGTGC
AACAAGGAGATAGTGTATAT

Product: isochorismatase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MNFSSENTAVVFVDLINDFQFDGGDDLLHHTKQILPNVIKLRSFAKENNLPIIYVNDHYGLWQADFNKVIDYCTNEKSKE
IIDKIKPDEDDYFLIKPQHSAFFQTPLHSLLIELERNHVILAGIAGDICILFSAKDAYMYKIDMSIPKNCMASEQEENNN
YALYLMESVMDADISPI

Sequences:

>Translated_177_residues
MNFSSENTAVVFVDLINDFQFDGGDDLLHHTKQILPNVIKLRSFAKENNLPIIYVNDHYGLWQADFNKVIDYCTNEKSKE
IIDKIKPDEDDYFLIKPQHSAFFQTPLHSLLIELERNHVILAGIAGDICILFSAKDAYMYKIDMSIPKNCMASEQEENNN
YALYLMESVMDADISPI
>Mature_177_residues
MNFSSENTAVVFVDLINDFQFDGGDDLLHHTKQILPNVIKLRSFAKENNLPIIYVNDHYGLWQADFNKVIDYCTNEKSKE
IIDKIKPDEDDYFLIKPQHSAFFQTPLHSLLIELERNHVILAGIAGDICILFSAKDAYMYKIDMSIPKNCMASEQEENNN
YALYLMESVMDADISPI

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=147, Percent_Identity=26.530612244898, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 20357; Mature: 20357

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSSENTAVVFVDLINDFQFDGGDDLLHHTKQILPNVIKLRSFAKENNLPIIYVNDHYG
CCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LWQADFNKVIDYCTNEKSKEIIDKIKPDEDDYFLIKPQHSAFFQTPLHSLLIELERNHVI
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEE
LAGIAGDICILFSAKDAYMYKIDMSIPKNCMASEQEENNNYALYLMESVMDADISPI
EEECCCCEEEEEECCCEEEEEEECCCCCCHHCCCCCCCCCEEEEEEEHHHCCCCCCC
>Mature Secondary Structure
MNFSSENTAVVFVDLINDFQFDGGDDLLHHTKQILPNVIKLRSFAKENNLPIIYVNDHYG
CCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LWQADFNKVIDYCTNEKSKEIIDKIKPDEDDYFLIKPQHSAFFQTPLHSLLIELERNHVI
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEE
LAGIAGDICILFSAKDAYMYKIDMSIPKNCMASEQEENNNYALYLMESVMDADISPI
EEECCCCEEEEEECCCEEEEEEECCCCCCHHCCCCCCCCCEEEEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]