Click here to switch to the map view.

The map label for this gene is gltT [H]

Identifier: 23097476

GI number: 23097476

Start: 24172

End: 25398

Strand: Direct

Name: gltT [H]

Synonym: OB0021

Alternate gene names: 23097476

Gene position: 24172-25398 (Clockwise)

Preceding gene: 23097475

Following gene: 23097477

Centisome position: 0.67

GC content: 37.98

Gene sequence:

>1227_bases
ATGAGTTTAACAAAAAAGATATTAATTGCTCTTATTGCAGGTGCTGTTGTCGGAGGAATTTTAACATTTACACCTGATTC
TTTTTTTGGGCCTGTAGATAAGTTTTTTCTAAATCCAATTGGTCAAATATTCTTGAATTTAATAATGATGCTAGTTGTAC
CACTAGTATTTGTATCGATTACTTTAGGAACAGCTGGATTAGGTGAACCGGCTAAACTAGGTAAGATTGGTGTATCAACG
GTTGGGTTTTTCTTATGTACCACTGCACTTGCACTTACAATTGCATTAGGATTGGGTTATCTCATAAAGCCAGGGGAAGC
TGGGATGTTCGATACGGCGAATGCGAACTATGAGCCGAGCGAAGCTCCTCCGATTATGGAAACGTTAATAAACATCATTC
CAGAAAATCCAATTGCAGCAATGTCATCGGGAGACATGTTGCAAATAATCGCATTTGCTATATTTATTGGTATTGCTTTA
GCTTACCTTGGAGAGAAAACCCAAGGAATTCATAGTCTTTTTGAACAAGCAAATGAAATACTAAATTGGTTAGTAATGGT
AGTAATGAAATTCGCTCCTTATGGTGCTTTTGCTTTAATCGCTTCTGCTATAGGTGGTGCAGGATTAGACGCAGTAGGAT
CTATGGTGAGTTATATGTTAACAGTGATACTTGGATTAATCGTTCATGGACTATTAGTTTATGGGTTAATCATTTGGTTC
TTAGGAAAACAGAATCCATTTACCTTTTATAAAGGATTTTTCCCGGCTATGTCCGTTGCATTTAGTACTTCAAGTTCCAA
TGCAACGTTACCATTATCCATGAAGACAGCGCAGGAAAACCTAGGTGTGAAGAAAGAGATTAGTAGTTTTGTTCAACCTT
TGGGTGCAACAATTAACATGGATGGTACTGCTATTATGCAGGCAGTTGCCACTGTATTCATATCGCAGGTGTATGATATA
CCACTAGGCTTTTCAGAATTACTTATGGTTGTATTGGTTGCAACATTAGCAAGTGTTGGAACTGCAGGAGTTCCAGGTGT
GGGCTTGATTATGTTAGCAATGGTATTAAGTCAAGTAGGTTTACCAGCAGAAGGAATAGCGTTAATTGTTGGTATCGATC
GTATCTTAGATATGCTACGGACTTCATTAAACATTACCGGTGATGCCACTATTGCCTATGTACTGTCTGAAAGAGAAAAA
CGTAAAGAAGCAGCTAGTGCGAAATAG

Upstream 100 bases:

>100_bases
TATCCCAATGCCCAAACAAACATAATAGCTAAGAAGTAGAGATGATTTCACTTATATAAGAAAGAATTTTCTGAAAAAAC
AGAGAGGATGATATTACACC

Downstream 100 bases:

>100_bases
AAAAAAATTAGTAGGAAGAAAACCATTCGCTTTTAGAGTGGCTTTCTTCCTCTTTTTTTGTAAAAAGCGGACAAGGGACT
TTAAAAAAGTCACAAAATGT

Product: H(+):sodium-glutamate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSLTKKILIALIAGAVVGGILTFTPDSFFGPVDKFFLNPIGQIFLNLIMMLVVPLVFVSITLGTAGLGEPAKLGKIGVST
VGFFLCTTALALTIALGLGYLIKPGEAGMFDTANANYEPSEAPPIMETLINIIPENPIAAMSSGDMLQIIAFAIFIGIAL
AYLGEKTQGIHSLFEQANEILNWLVMVVMKFAPYGAFALIASAIGGAGLDAVGSMVSYMLTVILGLIVHGLLVYGLIIWF
LGKQNPFTFYKGFFPAMSVAFSTSSSNATLPLSMKTAQENLGVKKEISSFVQPLGATINMDGTAIMQAVATVFISQVYDI
PLGFSELLMVVLVATLASVGTAGVPGVGLIMLAMVLSQVGLPAEGIALIVGIDRILDMLRTSLNITGDATIAYVLSEREK
RKEAASAK

Sequences:

>Translated_408_residues
MSLTKKILIALIAGAVVGGILTFTPDSFFGPVDKFFLNPIGQIFLNLIMMLVVPLVFVSITLGTAGLGEPAKLGKIGVST
VGFFLCTTALALTIALGLGYLIKPGEAGMFDTANANYEPSEAPPIMETLINIIPENPIAAMSSGDMLQIIAFAIFIGIAL
AYLGEKTQGIHSLFEQANEILNWLVMVVMKFAPYGAFALIASAIGGAGLDAVGSMVSYMLTVILGLIVHGLLVYGLIIWF
LGKQNPFTFYKGFFPAMSVAFSTSSSNATLPLSMKTAQENLGVKKEISSFVQPLGATINMDGTAIMQAVATVFISQVYDI
PLGFSELLMVVLVATLASVGTAGVPGVGLIMLAMVLSQVGLPAEGIALIVGIDRILDMLRTSLNITGDATIAYVLSEREK
RKEAASAK
>Mature_407_residues
SLTKKILIALIAGAVVGGILTFTPDSFFGPVDKFFLNPIGQIFLNLIMMLVVPLVFVSITLGTAGLGEPAKLGKIGVSTV
GFFLCTTALALTIALGLGYLIKPGEAGMFDTANANYEPSEAPPIMETLINIIPENPIAAMSSGDMLQIIAFAIFIGIALA
YLGEKTQGIHSLFEQANEILNWLVMVVMKFAPYGAFALIASAIGGAGLDAVGSMVSYMLTVILGLIVHGLLVYGLIIWFL
GKQNPFTFYKGFFPAMSVAFSTSSSNATLPLSMKTAQENLGVKKEISSFVQPLGATINMDGTAIMQAVATVFISQVYDIP
LGFSELLMVVLVATLASVGTAGVPGVGLIMLAMVLSQVGLPAEGIALIVGIDRILDMLRTSLNITGDATIAYVLSEREKR
KEAASAK

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=449, Percent_Identity=33.630289532294, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI66773030, Length=461, Percent_Identity=31.6702819956616, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI21314632, Length=407, Percent_Identity=33.4152334152334, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI194239697, Length=460, Percent_Identity=30.6521739130435, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI40254478, Length=464, Percent_Identity=32.7586206896552, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI5032093, Length=429, Percent_Identity=35.6643356643357, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI223468564, Length=268, Percent_Identity=40.2985074626866, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI223468566, Length=268, Percent_Identity=40.2985074626866, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI4827012, Length=276, Percent_Identity=39.1304347826087, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI262359914, Length=377, Percent_Identity=31.2997347480106, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI301601644, Length=185, Percent_Identity=39.4594594594595, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI1790514, Length=417, Percent_Identity=32.8537170263789, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1789947, Length=373, Percent_Identity=30.8310991957105, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1788024, Length=393, Percent_Identity=29.0076335877863, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1789473, Length=296, Percent_Identity=28.0405405405405, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71983106, Length=436, Percent_Identity=32.7981651376147, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI71983099, Length=436, Percent_Identity=32.7981651376147, Blast_Score=224, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI193206505, Length=413, Percent_Identity=31.7191283292978, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71996953, Length=453, Percent_Identity=31.7880794701987, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17537407, Length=429, Percent_Identity=31.2354312354312, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI193206654, Length=400, Percent_Identity=29.25, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17541374, Length=392, Percent_Identity=30.3571428571429, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24583025, Length=426, Percent_Identity=29.1079812206573, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17137668, Length=426, Percent_Identity=29.1079812206573, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24583023, Length=426, Percent_Identity=29.1079812206573, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI281360483, Length=468, Percent_Identity=28.2051282051282, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17137666, Length=468, Percent_Identity=28.2051282051282, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI281360481, Length=468, Percent_Identity=28.4188034188034, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 42889; Mature: 42757

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTKKILIALIAGAVVGGILTFTPDSFFGPVDKFFLNPIGQIFLNLIMMLVVPLVFVSI
CCHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGTAGLGEPAKLGKIGVSTVGFFLCTTALALTIALGLGYLIKPGEAGMFDTANANYEPS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCC
EAPPIMETLINIIPENPIAAMSSGDMLQIIAFAIFIGIALAYLGEKTQGIHSLFEQANEI
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LNWLVMVVMKFAPYGAFALIASAIGGAGLDAVGSMVSYMLTVILGLIVHGLLVYGLIIWF
HHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGKQNPFTFYKGFFPAMSVAFSTSSSNATLPLSMKTAQENLGVKKEISSFVQPLGATINM
HCCCCCCHHHHHHHHHHHHHEECCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHCCEECC
DGTAIMQAVATVFISQVYDIPLGFSELLMVVLVATLASVGTAGVPGVGLIMLAMVLSQVG
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
LPAEGIALIVGIDRILDMLRTSLNITGDATIAYVLSEREKRKEAASAK
CCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLTKKILIALIAGAVVGGILTFTPDSFFGPVDKFFLNPIGQIFLNLIMMLVVPLVFVSI
CHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGTAGLGEPAKLGKIGVSTVGFFLCTTALALTIALGLGYLIKPGEAGMFDTANANYEPS
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCC
EAPPIMETLINIIPENPIAAMSSGDMLQIIAFAIFIGIALAYLGEKTQGIHSLFEQANEI
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LNWLVMVVMKFAPYGAFALIASAIGGAGLDAVGSMVSYMLTVILGLIVHGLLVYGLIIWF
HHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGKQNPFTFYKGFFPAMSVAFSTSSSNATLPLSMKTAQENLGVKKEISSFVQPLGATINM
HCCCCCCHHHHHHHHHHHHHEECCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHCCEECC
DGTAIMQAVATVFISQVYDIPLGFSELLMVVLVATLASVGTAGVPGVGLIMLAMVLSQVG
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
LPAEGIALIVGIDRILDMLRTSLNITGDATIAYVLSEREKRKEAASAK
CCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]