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The map label for this gene is ydgK [H]

Identifier: 23097472

GI number: 23097472

Start: 20453

End: 21691

Strand: Direct

Name: ydgK [H]

Synonym: OB0017

Alternate gene names: 23097472

Gene position: 20453-21691 (Clockwise)

Preceding gene: 23097469

Following gene: 23097473

Centisome position: 0.56

GC content: 38.5

Gene sequence:

>1239_bases
TTGCTTATTTCTCTCAAACATCAGGAAAAATCAAATTCAATCTTACATAACCCTACTGGAAAAGCGCGGATTGGGTTAGC
GTTATTACTAGGATTACTGGCTTTTTTAGGTCCGCTTAATATCGATATGTATTTGCCAAGCTTTCCAGGGATATCTTCAG
ATTTCGGCGTTAGTGCTACCCTTGTCCAATTTAGTTTGACTGCGTGTTTAATTGGACTAGCTGTCGGTCAAATAGTAGTA
GGACCGATAAGTGATGCACAAGGTAGGAAAAAACCATTATTAATTGCGACTTTCTTATTTGCCTTATCTTCGCTTCTTTG
TGCTTTTTCGCCTAATATCTCAACACTAATTGTTGCACGGTTCTTGCAAGGGTTAACTGCATCAGCAGGAGTTGTGTTAT
CTCGTGCAGTTGTACGTGATGTATTTACAGGCAAACAGCTGACAAAGTTTTTCGCTCTTTTAATGGTAATTAATGCGATA
GCACCAATGATAGCACCAATGATTGGTGGAGGAATATTAGCTATTCCATCTACAAATTGGCAATCTATCTTTTATTTCCT
TGCAATAATTGGCATTTTAATTGTTCTCATCGTTGCCATAAAATTAAAAGAAACATTGCCTCCACAAAAACGTATACCGA
GTTCAATTGGTTCTTCTGTGAAAACGATGGGGAGTTTACTCAAGGATCGTTCGTTTATCGGCTATGCATTAGTGGTTGGA
TTTGTGCATGGAGGAAGTTTCGCATATGTATCCGGTACGCCATTTGTATATCAAGATATCTATGGTGTTTCACCACAGGT
ATTCAGTGTCTTATTTGGAATCAATGGACTTGCAATTATTACTGGAAGCTTTCTCATCGGACGATTGAGTGATATTGTCT
CAGAGAAAAAACTTCTACAGACAGCAGTTACTATTGCACTTACTGCAACCTCCCTGCTTTTACTAATGACAATTATAAAA
GGACCATTAGCGTCGTTAGTGATTTTAATCTTTATATACATGATTACGATGGGGATGACGATTACAAGTACATTTACTTT
AGGTATGGCTAAACAAGGGCATCGTGCAGGAAGTGCGAGTGCGGTATTAGGCATGCTTCCAATGTTACTTGGTGCGTTAT
TTTCTCCGCTTGCGGGTATTAATGAAGCATCTGCCGTGCCGATGGGGATGATATTGTTTACAACATCGCTGATCGGTTTC
ATTGCTTTCTTCACATTGGTTAAAAAAAATGAAAATTAA

Upstream 100 bases:

>100_bases
TTCTACCATAAATCGACATTATCTACTACAAGACAACAAATAGTATACATTTCTTGACAGGAAGCGTATGGACGATTAAA
ATTCTTCCATTAAGGAGTGA

Downstream 100 bases:

>100_bases
AAAGGGTAGTATATTCAGTGATTTGTCAGCTTTTCAAATAGTGGAGTATTTAGTATAATAGCTTCTGTTATTACCTTTTA
TGCCCTCGTAGCTCAGGGGA

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MLISLKHQEKSNSILHNPTGKARIGLALLLGLLAFLGPLNIDMYLPSFPGISSDFGVSATLVQFSLTACLIGLAVGQIVV
GPISDAQGRKKPLLIATFLFALSSLLCAFSPNISTLIVARFLQGLTASAGVVLSRAVVRDVFTGKQLTKFFALLMVINAI
APMIAPMIGGGILAIPSTNWQSIFYFLAIIGILIVLIVAIKLKETLPPQKRIPSSIGSSVKTMGSLLKDRSFIGYALVVG
FVHGGSFAYVSGTPFVYQDIYGVSPQVFSVLFGINGLAIITGSFLIGRLSDIVSEKKLLQTAVTIALTATSLLLLMTIIK
GPLASLVILIFIYMITMGMTITSTFTLGMAKQGHRAGSASAVLGMLPMLLGALFSPLAGINEASAVPMGMILFTTSLIGF
IAFFTLVKKNEN

Sequences:

>Translated_412_residues
MLISLKHQEKSNSILHNPTGKARIGLALLLGLLAFLGPLNIDMYLPSFPGISSDFGVSATLVQFSLTACLIGLAVGQIVV
GPISDAQGRKKPLLIATFLFALSSLLCAFSPNISTLIVARFLQGLTASAGVVLSRAVVRDVFTGKQLTKFFALLMVINAI
APMIAPMIGGGILAIPSTNWQSIFYFLAIIGILIVLIVAIKLKETLPPQKRIPSSIGSSVKTMGSLLKDRSFIGYALVVG
FVHGGSFAYVSGTPFVYQDIYGVSPQVFSVLFGINGLAIITGSFLIGRLSDIVSEKKLLQTAVTIALTATSLLLLMTIIK
GPLASLVILIFIYMITMGMTITSTFTLGMAKQGHRAGSASAVLGMLPMLLGALFSPLAGINEASAVPMGMILFTTSLIGF
IAFFTLVKKNEN
>Mature_412_residues
MLISLKHQEKSNSILHNPTGKARIGLALLLGLLAFLGPLNIDMYLPSFPGISSDFGVSATLVQFSLTACLIGLAVGQIVV
GPISDAQGRKKPLLIATFLFALSSLLCAFSPNISTLIVARFLQGLTASAGVVLSRAVVRDVFTGKQLTKFFALLMVINAI
APMIAPMIGGGILAIPSTNWQSIFYFLAIIGILIVLIVAIKLKETLPPQKRIPSSIGSSVKTMGSLLKDRSFIGYALVVG
FVHGGSFAYVSGTPFVYQDIYGVSPQVFSVLFGINGLAIITGSFLIGRLSDIVSEKKLLQTAVTIALTATSLLLLMTIIK
GPLASLVILIFIYMITMGMTITSTFTLGMAKQGHRAGSASAVLGMLPMLLGALFSPLAGINEASAVPMGMILFTTSLIGF
IAFFTLVKKNEN

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=394, Percent_Identity=31.2182741116751, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI48994889, Length=252, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790146, Length=353, Percent_Identity=27.1954674220963, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87082312, Length=257, Percent_Identity=28.4046692607004, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790794, Length=342, Percent_Identity=22.2222222222222, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787065, Length=350, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319657, Length=171, Percent_Identity=30.9941520467836, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325414, Length=154, Percent_Identity=26.6233766233766, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321577, Length=157, Percent_Identity=28.0254777070064, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43507; Mature: 43507

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISLKHQEKSNSILHNPTGKARIGLALLLGLLAFLGPLNIDMYLPSFPGISSDFGVSAT
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHH
LVQFSLTACLIGLAVGQIVVGPISDAQGRKKPLLIATFLFALSSLLCAFSPNISTLIVAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FLQGLTASAGVVLSRAVVRDVFTGKQLTKFFALLMVINAIAPMIAPMIGGGILAIPSTNW
HHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCH
QSIFYFLAIIGILIVLIVAIKLKETLPPQKRIPSSIGSSVKTMGSLLKDRSFIGYALVVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHH
FVHGGSFAYVSGTPFVYQDIYGVSPQVFSVLFGINGLAIITGSFLIGRLSDIVSEKKLLQ
HHCCCCEEEECCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAVTIALTATSLLLLMTIIKGPLASLVILIFIYMITMGMTITSTFTLGMAKQGHRAGSAS
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHH
AVLGMLPMLLGALFSPLAGINEASAVPMGMILFTTSLIGFIAFFTLVKKNEN
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLISLKHQEKSNSILHNPTGKARIGLALLLGLLAFLGPLNIDMYLPSFPGISSDFGVSAT
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHH
LVQFSLTACLIGLAVGQIVVGPISDAQGRKKPLLIATFLFALSSLLCAFSPNISTLIVAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FLQGLTASAGVVLSRAVVRDVFTGKQLTKFFALLMVINAIAPMIAPMIGGGILAIPSTNW
HHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCH
QSIFYFLAIIGILIVLIVAIKLKETLPPQKRIPSSIGSSVKTMGSLLKDRSFIGYALVVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHH
FVHGGSFAYVSGTPFVYQDIYGVSPQVFSVLFGINGLAIITGSFLIGRLSDIVSEKKLLQ
HHCCCCEEEECCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAVTIALTATSLLLLMTIIKGPLASLVILIFIYMITMGMTITSTFTLGMAKQGHRAGSAS
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHH
AVLGMLPMLLGALFSPLAGINEASAVPMGMILFTTSLIGFIAFFTLVKKNEN
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]