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The map label for this gene is sigD [H]

Identifier: 229918653

GI number: 229918653

Start: 2898888

End: 2899658

Strand: Direct

Name: sigD [H]

Synonym: EAT1b_2940

Alternate gene names: 229918653

Gene position: 2898888-2899658 (Clockwise)

Preceding gene: 229918652

Following gene: 229918654

Centisome position: 96.63

GC content: 45.27

Gene sequence:

>771_bases
ATGACGACTCATCTTACACAGTTGTGGGATCGCTGGAATATGGACCGTGATATGGATACAGCAAACGAATTACTAGCACA
TTACGAGTTTCTCATCCACTACCACGTACAACGGATGATCGTGACGCTTCCAAAGAGCGTCGAACGCGACGAATTGAAAA
GTTTGGGTTACATGGGATTGTACGATGCCCTTATGAAATATGATCCAGAGCACAACAATAAATTTGATACGTATGCTGCG
TTTCGGATACGAGGCGCCATAATCGACGGCTTACGAAAAGTGGACTGGTTACCTCGGTCGGTTCGAGAAAAAGTAAAAAA
AATCGATCACGTGGCAGAACAGCTTGAACAGTCGTTACATCGAGCTCCGACGAAGAGCGAATTAGCTTCGGCATGCGACC
TGCCGATTTCAGAAATCGAACGAACGATGGCGGAAGGTTACTTTGCTAATATGTTGTCGATTGATGAGGCCGTGACGTTG
CAAGAAGAAGGGAAAGTCATGTCTGTCACGTATTTCGACCCGGATTCTGAAAAGCCTGAAGATACATTATTACAACGCGA
ATTGATTGACGTGCTGGCTGCAGAGGTTGACCATTTATCTGAAAAAGAGCGACTCGTCGTATCTTTGTTTTACTTTGACG
AACTGACGTTGACAGAGATTGGGGAAGTGCTCGAACTGTCCACATCGCGGATATCGCAAATTCATTCCAAAGCGTTGAAG
ACGTTAAAGCATGCGCTCGGTCGGTCCTATTTAGAAGAAAAGACATCGTAA

Upstream 100 bases:

>100_bases
CGGACATAACGGGCGGACGATCGAATTTGATGTCGCAACATCCGTCTTGTCGATTCGGACGGTGAGCGTGGGTACGAAAG
AACTATAAGGGGGGATCGAC

Downstream 100 bases:

>100_bases
GGAGATGAACAAGTGTATATTGTATATGTGATCACAGCTTGTTTGGTTGCTTACGGTTTTTTATTCGGCTACCGACAAGT
TCAAGCGCTGCAGGAACGAG

Product: RNA polymerase sigma factor SigD

Products: NA

Alternate protein names: Sigma-28 [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTTHLTQLWDRWNMDRDMDTANELLAHYEFLIHYHVQRMIVTLPKSVERDELKSLGYMGLYDALMKYDPEHNNKFDTYAA
FRIRGAIIDGLRKVDWLPRSVREKVKKIDHVAEQLEQSLHRAPTKSELASACDLPISEIERTMAEGYFANMLSIDEAVTL
QEEGKVMSVTYFDPDSEKPEDTLLQRELIDVLAAEVDHLSEKERLVVSLFYFDELTLTEIGEVLELSTSRISQIHSKALK
TLKHALGRSYLEEKTS

Sequences:

>Translated_256_residues
MTTHLTQLWDRWNMDRDMDTANELLAHYEFLIHYHVQRMIVTLPKSVERDELKSLGYMGLYDALMKYDPEHNNKFDTYAA
FRIRGAIIDGLRKVDWLPRSVREKVKKIDHVAEQLEQSLHRAPTKSELASACDLPISEIERTMAEGYFANMLSIDEAVTL
QEEGKVMSVTYFDPDSEKPEDTLLQRELIDVLAAEVDHLSEKERLVVSLFYFDELTLTEIGEVLELSTSRISQIHSKALK
TLKHALGRSYLEEKTS
>Mature_255_residues
TTHLTQLWDRWNMDRDMDTANELLAHYEFLIHYHVQRMIVTLPKSVERDELKSLGYMGLYDALMKYDPEHNNKFDTYAAF
RIRGAIIDGLRKVDWLPRSVREKVKKIDHVAEQLEQSLHRAPTKSELASACDLPISEIERTMAEGYFANMLSIDEAVTLQ
EEGKVMSVTYFDPDSEKPEDTLLQRELIDVLAAEVDHLSEKERLVVSLFYFDELTLTEIGEVLELSTSRISQIHSKALKT
LKHALGRSYLEEKTS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=225, Percent_Identity=37.7777777777778, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1789098, Length=199, Percent_Identity=25.6281407035176, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29650; Mature: 29519

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHLTQLWDRWNMDRDMDTANELLAHYEFLIHYHVQRMIVTLPKSVERDELKSLGYMGL
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHH
YDALMKYDPEHNNKFDTYAAFRIRGAIIDGLRKVDWLPRSVREKVKKIDHVAEQLEQSLH
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RAPTKSELASACDLPISEIERTMAEGYFANMLSIDEAVTLQEEGKVMSVTYFDPDSEKPE
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHH
DTLLQRELIDVLAAEVDHLSEKERLVVSLFYFDELTLTEIGEVLELSTSRISQIHSKALK
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLKHALGRSYLEEKTS
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTHLTQLWDRWNMDRDMDTANELLAHYEFLIHYHVQRMIVTLPKSVERDELKSLGYMGL
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHH
YDALMKYDPEHNNKFDTYAAFRIRGAIIDGLRKVDWLPRSVREKVKKIDHVAEQLEQSLH
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RAPTKSELASACDLPISEIERTMAEGYFANMLSIDEAVTLQEEGKVMSVTYFDPDSEKPE
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHH
DTLLQRELIDVLAAEVDHLSEKERLVVSLFYFDELTLTEIGEVLELSTSRISQIHSKALK
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLKHALGRSYLEEKTS
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377; 7602586; 8157612 [H]