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The map label for this gene is fliM [H]

Identifier: 229918638

GI number: 229918638

Start: 2885647

End: 2886636

Strand: Direct

Name: fliM [H]

Synonym: EAT1b_2925

Alternate gene names: 229918638

Gene position: 2885647-2886636 (Clockwise)

Preceding gene: 229918637

Following gene: 229918639

Centisome position: 96.19

GC content: 47.27

Gene sequence:

>990_bases
ATGGGAGAAGTACTGTCTCAACAAGAAATTGATGCCTTACTATCTGCATTAAGCAGTGGGGATGTCGAAGCGGACTCCTT
TCTCGCGCAAGAGGAAGAGAAGAAAGTTCGACAATATGACTTCAAGCGAGCCGTTCGATTTTCAAAAGATCAAATTCGTA
GCTTAACTCGGATTCACGAGCATTTCACACGGATGCTCACGACATTTTTCTCGGCCCAACTTCGCACGTACGTTCAGTTT
TCGGTCAACTCGGTCGAACAATTACCGTACGATGAATTCATCCATTCCATTCCGAGCATGACGATGATCAACTTGATTGA
GGCGCCACCGTTAAACGGGCGATTCATCATCGAGGTCAATCCGAACATCTCGTACGCGATGTTAGACCGATTACTCGGTG
GTCCCGGTGTCGAGATGGATAAAATCGAATCGTTCACCGAAATCGAGATGCGGATCTTGACGCAATTGTACAAACGGGCG
TTTTCGACATATGGGGATGCTTGGGAATCGATTGCTGAGATTAAAGCAGAAATGTCAGCCGTTGAAGTGAATCCGCAGTT
CCTGCAACTCGTCTCACCAAATGAAACGGTCGTTTTGATTTCAATCGGTGTGACGATCGGTGAAGTGAGCGGAACGATCA
ACGTCTGTCTGCCATTCGTCACAATCGAACCGGTATTGTCCAAATTATCGAGTCATTACTGGATGCAGGAAGCGAATCGA
CGCAATGCGAGTCAATCGAGTAAGGAACCGCTTAAAGCCCAATTGATGAACTCGACGGTCGAGGTCGTATCCTTACTCGG
TGAGACGACGATCACATTTGGAGATTTGTTGCATCTAGAAGTCGGAGATTGTTTGGCACTCGATCAATTAGCAAAAGAAC
CCCTGTCCGTCATGGTGGGGGAAAATAAAGTATTTAAAGGGCAAGTCGGTGTGAGCGGGAAGCGAATGGCGGTTCAAGTG
CTTCACCGTGTGAAGGAGGAAGAGCAATGA

Upstream 100 bases:

>100_bases
GTTCGAACAAACATTACGAGAACAACTTAACGGACTGCTTGAAGAAGGTGAAGTCCAACGCGTCTACATGACGAAGAAAA
TTATTCAGTGAGGTGGCATC

Downstream 100 bases:

>100_bases
GTGAGATGCTGTCACAAGATGAAATTGATGCGTTATTACGCGGGACAAGCGACAATACGCCGACGCCAGAAGTCGATGCG
AAAGAAGAACATTTAGATTC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MGEVLSQQEIDALLSALSSGDVEADSFLAQEEEKKVRQYDFKRAVRFSKDQIRSLTRIHEHFTRMLTTFFSAQLRTYVQF
SVNSVEQLPYDEFIHSIPSMTMINLIEAPPLNGRFIIEVNPNISYAMLDRLLGGPGVEMDKIESFTEIEMRILTQLYKRA
FSTYGDAWESIAEIKAEMSAVEVNPQFLQLVSPNETVVLISIGVTIGEVSGTINVCLPFVTIEPVLSKLSSHYWMQEANR
RNASQSSKEPLKAQLMNSTVEVVSLLGETTITFGDLLHLEVGDCLALDQLAKEPLSVMVGENKVFKGQVGVSGKRMAVQV
LHRVKEEEQ

Sequences:

>Translated_329_residues
MGEVLSQQEIDALLSALSSGDVEADSFLAQEEEKKVRQYDFKRAVRFSKDQIRSLTRIHEHFTRMLTTFFSAQLRTYVQF
SVNSVEQLPYDEFIHSIPSMTMINLIEAPPLNGRFIIEVNPNISYAMLDRLLGGPGVEMDKIESFTEIEMRILTQLYKRA
FSTYGDAWESIAEIKAEMSAVEVNPQFLQLVSPNETVVLISIGVTIGEVSGTINVCLPFVTIEPVLSKLSSHYWMQEANR
RNASQSSKEPLKAQLMNSTVEVVSLLGETTITFGDLLHLEVGDCLALDQLAKEPLSVMVGENKVFKGQVGVSGKRMAVQV
LHRVKEEEQ
>Mature_328_residues
GEVLSQQEIDALLSALSSGDVEADSFLAQEEEKKVRQYDFKRAVRFSKDQIRSLTRIHEHFTRMLTTFFSAQLRTYVQFS
VNSVEQLPYDEFIHSIPSMTMINLIEAPPLNGRFIIEVNPNISYAMLDRLLGGPGVEMDKIESFTEIEMRILTQLYKRAF
STYGDAWESIAEIKAEMSAVEVNPQFLQLVSPNETVVLISIGVTIGEVSGTINVCLPFVTIEPVLSKLSSHYWMQEANRR
NASQSSKEPLKAQLMNSTVEVVSLLGETTITFGDLLHLEVGDCLALDQLAKEPLSVMVGENKVFKGQVGVSGKRMAVQVL
HRVKEEEQ

Specific function: One of the proteins that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with chemotaxis proteins (such as CheY) in addition to contacting components of the motor that determine the direction of f

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=337, Percent_Identity=29.080118694362, Blast_Score=147, Evalue=8e-37,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36912; Mature: 36781

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVLSQQEIDALLSALSSGDVEADSFLAQEEEKKVRQYDFKRAVRFSKDQIRSLTRIHE
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HFTRMLTTFFSAQLRTYVQFSVNSVEQLPYDEFIHSIPSMTMINLIEAPPLNGRFIIEVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEC
PNISYAMLDRLLGGPGVEMDKIESFTEIEMRILTQLYKRAFSTYGDAWESIAEIKAEMSA
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEVNPQFLQLVSPNETVVLISIGVTIGEVSGTINVCLPFVTIEPVLSKLSSHYWMQEANR
EECCHHHHEEECCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNASQSSKEPLKAQLMNSTVEVVSLLGETTITFGDLLHLEVGDCLALDQLAKEPLSVMVG
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHEEEECCCCHHHHHHHHHCCHHEEEC
ENKVFKGQVGVSGKRMAVQVLHRVKEEEQ
CCEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GEVLSQQEIDALLSALSSGDVEADSFLAQEEEKKVRQYDFKRAVRFSKDQIRSLTRIHE
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HFTRMLTTFFSAQLRTYVQFSVNSVEQLPYDEFIHSIPSMTMINLIEAPPLNGRFIIEVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEC
PNISYAMLDRLLGGPGVEMDKIESFTEIEMRILTQLYKRAFSTYGDAWESIAEIKAEMSA
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEVNPQFLQLVSPNETVVLISIGVTIGEVSGTINVCLPFVTIEPVLSKLSSHYWMQEANR
EECCHHHHEEECCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNASQSSKEPLKAQLMNSTVEVVSLLGETTITFGDLLHLEVGDCLALDQLAKEPLSVMVG
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHEEEECCCCHHHHHHHHHCCHHEEEC
ENKVFKGQVGVSGKRMAVQVLHRVKEEEQ
CCEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898932; 9384377; 1828465; 1447979 [H]