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The map label for this gene is pyrAA [H]

Identifier: 229918567

GI number: 229918567

Start: 2817509

End: 2818591

Strand: Direct

Name: pyrAA [H]

Synonym: EAT1b_2854

Alternate gene names: 229918567

Gene position: 2817509-2818591 (Clockwise)

Preceding gene: 229918566

Following gene: 229918568

Centisome position: 93.92

GC content: 51.89

Gene sequence:

>1083_bases
ATGAAACGAACATTATGGCTTGAAGATGGAATGAAATTTGTCGGTAAAGGATTCGGGGCACCGGCTGATACGATGGGGGA
AGTCGTCTTCCAGACAGGAATGACAGGATACCAAGAAATCTTATCGGATCCATCATACTGCGACCAAATGATTGTACTGA
CGAACCCATTGATCGGGAACTATGGCGTCAATCGTGAGGATTTTGAAACGACGATTCCGATGGCAAAAGCGTTGATTGTA
AAAGAAGTATGCGATACGCCTTCGAACTTCCGTTCAACGGCGACACTCGATGCGACACTCACCCGCTTCGGCATTCCGGG
GCTCTCGGATATCGATACACGGATGTTGACACGTCATTTACGAGCGAAAGGGGTCATGCGCGGTGTCCTCATCGATGGAG
AGTTGACGTGGGACGAGGTACGGGTCCGCTTTGATGAAGAATCGCCGAACGATCAAGTCGCACGCGTATCGACGAAGCGG
ACATATGTGAGTACGGGAACCGGCCCTCGCATCGCTTTGATCGACTTCGGGGCGAAACTTGGAATCATGCGCGAGTTGAT
GAAACGAGATTGTGAGGTCGTCGTCTTCCCGCACGATGTGACGGCAGAGGACGTATTACGGTATGAGCCGGACGGTATCA
TGTTGTCAAATGGACCAGGAGATCCGAAAGACGTTCCGACCGCACTGCCGCTCATCGAGCAATTGATGCCGACGACACCG
ATGTTCGGAATTTGTCTCGGTCACCAGCTGATTGCTCTCGCAAACGGTGCGAACACCTTCAAGCTTCCGTTCGGACATCG
TGGCGCGAATCATCCGGTGCTCGAGCTTGAGACTGGTGCCGTGACCATCACTTCTCAAAACCATGGATATGCGGTTGAGG
AAACGTCACTGCTCGATACACCGCTCATCGTCACACACCGTGCGGTCAATGACGGAACGATCGAAGGGGTAAGACATCTC
CATCACCCGACCTTCTCGGTACAGTATCATCCGGAGGCAGCACCGGGACCGTTTGACGCCAACGACTTATTTGACCGATT
CATGCAACTCGTACAAGAAAACAAACAGAAAGTAGGAATTTGA

Upstream 100 bases:

>100_bases
TATTCAAAAGGAAAGAATACACCGTTCGCAGGGGAAAAGCTTGTCGGATGGCCGGTCATGACGATGGTCGGTGGCGAAAT
TGTATATAAGGGGCGATTTG

Downstream 100 bases:

>100_bases
CATGCCAAAACGACCAGATATTCAGAAAATCCTCGTAATCGGATCAGGACCGATTGTAATCGGACAAGCAGCGGAGTTTG
ATTATGCTGGTACACAGGCA

Product: carbamoyl-phosphate synthase, small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKRTLWLEDGMKFVGKGFGAPADTMGEVVFQTGMTGYQEILSDPSYCDQMIVLTNPLIGNYGVNREDFETTIPMAKALIV
KEVCDTPSNFRSTATLDATLTRFGIPGLSDIDTRMLTRHLRAKGVMRGVLIDGELTWDEVRVRFDEESPNDQVARVSTKR
TYVSTGTGPRIALIDFGAKLGIMRELMKRDCEVVVFPHDVTAEDVLRYEPDGIMLSNGPGDPKDVPTALPLIEQLMPTTP
MFGICLGHQLIALANGANTFKLPFGHRGANHPVLELETGAVTITSQNHGYAVEETSLLDTPLIVTHRAVNDGTIEGVRHL
HHPTFSVQYHPEAAPGPFDANDLFDRFMQLVQENKQKVGI

Sequences:

>Translated_360_residues
MKRTLWLEDGMKFVGKGFGAPADTMGEVVFQTGMTGYQEILSDPSYCDQMIVLTNPLIGNYGVNREDFETTIPMAKALIV
KEVCDTPSNFRSTATLDATLTRFGIPGLSDIDTRMLTRHLRAKGVMRGVLIDGELTWDEVRVRFDEESPNDQVARVSTKR
TYVSTGTGPRIALIDFGAKLGIMRELMKRDCEVVVFPHDVTAEDVLRYEPDGIMLSNGPGDPKDVPTALPLIEQLMPTTP
MFGICLGHQLIALANGANTFKLPFGHRGANHPVLELETGAVTITSQNHGYAVEETSLLDTPLIVTHRAVNDGTIEGVRHL
HHPTFSVQYHPEAAPGPFDANDLFDRFMQLVQENKQKVGI
>Mature_360_residues
MKRTLWLEDGMKFVGKGFGAPADTMGEVVFQTGMTGYQEILSDPSYCDQMIVLTNPLIGNYGVNREDFETTIPMAKALIV
KEVCDTPSNFRSTATLDATLTRFGIPGLSDIDTRMLTRHLRAKGVMRGVLIDGELTWDEVRVRFDEESPNDQVARVSTKR
TYVSTGTGPRIALIDFGAKLGIMRELMKRDCEVVVFPHDVTAEDVLRYEPDGIMLSNGPGDPKDVPTALPLIEQLMPTTP
MFGICLGHQLIALANGANTFKLPFGHRGANHPVLELETGAVTITSQNHGYAVEETSLLDTPLIVTHRAVNDGTIEGVRHL
HHPTFSVQYHPEAAPGPFDANDLFDRFMQLVQENKQKVGI

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=364, Percent_Identity=44.7802197802198, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI21361331, Length=367, Percent_Identity=35.9673024523161, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI169790915, Length=367, Percent_Identity=35.9673024523161, Blast_Score=214, Evalue=7e-56,
Organism=Escherichia coli, GI1786215, Length=375, Percent_Identity=44.8, Blast_Score=308, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI193204318, Length=379, Percent_Identity=41.1609498680739, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=371, Percent_Identity=39.0835579514825, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=400, Percent_Identity=40, Blast_Score=236, Evalue=3e-63,
Organism=Drosophila melanogaster, GI45555749, Length=376, Percent_Identity=40.6914893617021, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24642586, Length=376, Percent_Identity=40.6914893617021, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39709; Mature: 39709

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTLWLEDGMKFVGKGFGAPADTMGEVVFQTGMTGYQEILSDPSYCDQMIVLTNPLIGN
CCCEEEHHHCHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCHHHHCEEEEEECCCCCC
YGVNREDFETTIPMAKALIVKEVCDTPSNFRSTATLDATLTRFGIPGLSDIDTRMLTRHL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHH
RAKGVMRGVLIDGELTWDEVRVRFDEESPNDQVARVSTKRTYVSTGTGPRIALIDFGAKL
HHHHHHEEEEECCCEEHHHEEEEECCCCCCCCEEEEEECEEEEECCCCCEEEEEECCHHH
GIMRELMKRDCEVVVFPHDVTAEDVLRYEPDGIMLSNGPGDPKDVPTALPLIEQLMPTTP
HHHHHHHHCCCCEEEECCCCCHHHHHEECCCCEEEECCCCCCHHCCHHHHHHHHHCCCCC
MFGICLGHQLIALANGANTFKLPFGHRGANHPVLELETGAVTITSQNHGYAVEETSLLDT
HHHHHHHHHHEEEECCCCEEECCCCCCCCCCCEEEEECCEEEEEECCCCEEEECCCCCCC
PLIVTHRAVNDGTIEGVRHLHHPTFSVQYHPEAAPGPFDANDLFDRFMQLVQENKQKVGI
CEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRTLWLEDGMKFVGKGFGAPADTMGEVVFQTGMTGYQEILSDPSYCDQMIVLTNPLIGN
CCCEEEHHHCHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCHHHHCEEEEEECCCCCC
YGVNREDFETTIPMAKALIVKEVCDTPSNFRSTATLDATLTRFGIPGLSDIDTRMLTRHL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHH
RAKGVMRGVLIDGELTWDEVRVRFDEESPNDQVARVSTKRTYVSTGTGPRIALIDFGAKL
HHHHHHEEEEECCCEEHHHEEEEECCCCCCCCEEEEEECEEEEECCCCCEEEEEECCHHH
GIMRELMKRDCEVVVFPHDVTAEDVLRYEPDGIMLSNGPGDPKDVPTALPLIEQLMPTTP
HHHHHHHHCCCCEEEECCCCCHHHHHEECCCCEEEECCCCCCHHCCHHHHHHHHHCCCCC
MFGICLGHQLIALANGANTFKLPFGHRGANHPVLELETGAVTITSQNHGYAVEETSLLDT
HHHHHHHHHHEEEECCCCEEECCCCCCCCCCCEEEEECCEEEEEECCCCEEEECCCCCCC
PLIVTHRAVNDGTIEGVRHLHHPTFSVQYHPEAAPGPFDANDLFDRFMQLVQENKQKVGI
CEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8206848 [H]