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The map label for this gene is pyrB [H]

Identifier: 229918565

GI number: 229918565

Start: 2815340

End: 2816254

Strand: Direct

Name: pyrB [H]

Synonym: EAT1b_2852

Alternate gene names: 229918565

Gene position: 2815340-2816254 (Clockwise)

Preceding gene: 229918564

Following gene: 229918566

Centisome position: 93.85

GC content: 51.48

Gene sequence:

>915_bases
ATGAATACGATGCAAACAAACGTGAAACCGGTCAAACACTTCGTCAGTTTGGACACACTTTCCCTTGAGGAGATCATGAG
CATGATTGAAGAATCATTGCGCTACAAGCAAGGTGACCTCCCCCCACTATTTGATGGGAAAACGGTCGCAAACCTGTTCT
TTGAAAACTCGACGCGTACGAAAAATTCGTTTCAAATGGCCGAGCGCCACTTGAACATGCAAGAGATTCCGTTCGATGTG
ACGACGTCATCGGTCACGAAAGGGGAGACGCTCTACGATACGTGTAAAACGCTCGAAGCCATCGGCGTGGACGCGCTCGT
CATTCGACATCCGGAAGCAGGCTATTATCACGAACTTGTGGAGAAGTTGAATATCCCAGTGTTGAACGGAGGGGACGGGA
GCGGGGAACATCCATCCCAATCATTGCTCGATCTCGTCACCATCTTTGAAGAGTTCGGAACGTTCCAAGGATTGAACGTC
GCCATCTGTGGTGACCTCGTCCATAGTCGGGTCGCCCGCTCGAACGCAAGCGTCTTGAAGCGCCTCGGAGCAAACGTGGT
CGGTTGTAGTCCTGCCTCTTGGTGGGATGACTCGATGGGGCTAGAGCGAAAAGAACTCGATGACATCCTAGGCGAATGTG
ACGTCATCATGTTGCTCCGTGTACAACACGAGCGCCATATCATGGGAGACGTCTTCTCGAAAGAAGCGTATCACACACAG
TTCGGTTTGACGGTCGAACGCGTTGAGCGAATGAAAGCAGGCGCCATCATCATGCACCCGGCTCCGGTCAATCGGGATGT
AGAGATTGCCGGAAGTCTGATCGAACATGAACGGTCACGCATCTTCCCACAAATGAAGAATGGGGTGTACGCCCGGATGA
GCATCTTAAATCGAGCATTAGGAGGACAACAATGA

Upstream 100 bases:

>100_bases
GCTCGTTTATTTGGGTGTACCCTGCCCAGATAAAGGCGTCCCTTCACATCGGTGAAGAGGCGCCTTTTCTCTTTGCCTCT
AATAAAAGGAGGAGACGAAC

Downstream 100 bases:

>100_bases
TACTTCAAAACGCGACATGGTATGACAAGGGAACGAAGCGCACGACAAACATTCACGTGAAAGACGGAAAGATCGAATCA
CTCGACTATAAAGGGGAGAC

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNTMQTNVKPVKHFVSLDTLSLEEIMSMIEESLRYKQGDLPPLFDGKTVANLFFENSTRTKNSFQMAERHLNMQEIPFDV
TTSSVTKGETLYDTCKTLEAIGVDALVIRHPEAGYYHELVEKLNIPVLNGGDGSGEHPSQSLLDLVTIFEEFGTFQGLNV
AICGDLVHSRVARSNASVLKRLGANVVGCSPASWWDDSMGLERKELDDILGECDVIMLLRVQHERHIMGDVFSKEAYHTQ
FGLTVERVERMKAGAIIMHPAPVNRDVEIAGSLIEHERSRIFPQMKNGVYARMSILNRALGGQQ

Sequences:

>Translated_304_residues
MNTMQTNVKPVKHFVSLDTLSLEEIMSMIEESLRYKQGDLPPLFDGKTVANLFFENSTRTKNSFQMAERHLNMQEIPFDV
TTSSVTKGETLYDTCKTLEAIGVDALVIRHPEAGYYHELVEKLNIPVLNGGDGSGEHPSQSLLDLVTIFEEFGTFQGLNV
AICGDLVHSRVARSNASVLKRLGANVVGCSPASWWDDSMGLERKELDDILGECDVIMLLRVQHERHIMGDVFSKEAYHTQ
FGLTVERVERMKAGAIIMHPAPVNRDVEIAGSLIEHERSRIFPQMKNGVYARMSILNRALGGQQ
>Mature_304_residues
MNTMQTNVKPVKHFVSLDTLSLEEIMSMIEESLRYKQGDLPPLFDGKTVANLFFENSTRTKNSFQMAERHLNMQEIPFDV
TTSSVTKGETLYDTCKTLEAIGVDALVIRHPEAGYYHELVEKLNIPVLNGGDGSGEHPSQSLLDLVTIFEEFGTFQGLNV
AICGDLVHSRVARSNASVLKRLGANVVGCSPASWWDDSMGLERKELDDILGECDVIMLLRVQHERHIMGDVFSKEAYHTQ
FGLTVERVERMKAGAIIMHPAPVNRDVEIAGSLIEHERSRIFPQMKNGVYARMSILNRALGGQQ

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=270, Percent_Identity=36.6666666666667, Blast_Score=160, Evalue=1e-39,
Organism=Escherichia coli, GI2367364, Length=300, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=36.4485981308411, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=273, Percent_Identity=35.5311355311355, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24642586, Length=320, Percent_Identity=33.75, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33992; Mature: 33992

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMQTNVKPVKHFVSLDTLSLEEIMSMIEESLRYKQGDLPPLFDGKTVANLFFENSTRT
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
KNSFQMAERHLNMQEIPFDVTTSSVTKGETLYDTCKTLEAIGVDALVIRHPEAGYYHELV
HHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
EKLNIPVLNGGDGSGEHPSQSLLDLVTIFEEFGTFQGLNVAICGDLVHSRVARSNASVLK
HHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLGANVVGCSPASWWDDSMGLERKELDDILGECDVIMLLRVQHERHIMGDVFSKEAYHTQ
HHCCCEEECCCCCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLTVERVERMKAGAIIMHPAPVNRDVEIAGSLIEHERSRIFPQMKNGVYARMSILNRAL
HCCHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHH
GGQQ
CCCC
>Mature Secondary Structure
MNTMQTNVKPVKHFVSLDTLSLEEIMSMIEESLRYKQGDLPPLFDGKTVANLFFENSTRT
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
KNSFQMAERHLNMQEIPFDVTTSSVTKGETLYDTCKTLEAIGVDALVIRHPEAGYYHELV
HHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
EKLNIPVLNGGDGSGEHPSQSLLDLVTIFEEFGTFQGLNVAICGDLVHSRVARSNASVLK
HHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLGANVVGCSPASWWDDSMGLERKELDDILGECDVIMLLRVQHERHIMGDVFSKEAYHTQ
HHCCCEEECCCCCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLTVERVERMKAGAIIMHPAPVNRDVEIAGSLIEHERSRIFPQMKNGVYARMSILNRAL
HCCHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHH
GGQQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA