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The map label for this gene is cofF [H]

Identifier: 229918532

GI number: 229918532

Start: 2784666

End: 2785433

Strand: Reverse

Name: cofF [H]

Synonym: EAT1b_2819

Alternate gene names: 229918532

Gene position: 2785433-2784666 (Counterclockwise)

Preceding gene: 229918538

Following gene: 229918531

Centisome position: 92.85

GC content: 46.88

Gene sequence:

>768_bases
ATGCGTCTACGTATGAAAAAAGTTTCTTTTAATATGATCCGATCGGCCGGGATCAAGACGGATTATGCGAAACCCGAATA
TTATTTCGATCAGCTCGACAAGGTTCGCGCTGCCGACCTCGTCCTGTTCCCTGAGTATTGGATGGTCAATTCCATCATCT
ACGGGATGAATAAAGCGATTTTCCCATCACCAGCGACTTATCACCTCGGGCACGACAAGATCGAGATGACGCGCGCCTTC
AAGGCGACATATCCGCACCGGATTCCGAAGACATTGATTTATGGAAAAAACCCATACACGATTGAACGCGTGTTAGAAGA
GTTCCACTACCCGTTCGTCGCCAAAAAAGCCAAATCCTCAATGGGTCAAGGGGTATGGTTAATCAAAAATGACCAAGACT
GGTTAGAATATGTCGAGAATCATGATACGTTCTATGTCCAAGAGTTCATCCCGAACGATCGTGACTTACGCGTCATCGTG
ATTGGAGAAGAAGTCGTCGGTGCCTATTGGCGTGTCAGTGAGGCCTTTTTAAACAACGTCGCTCAAGGAGCCCACTTCTC
GTACGAAGACATCCCGGAAGACGTCGTCCAAGAAGTTCAATCGATTGCGAATACGTTATCCATCAATCACGCAGCGTTTG
ACGTCATCGTGACAGAAGATGGATTTTACATTTTGGAATTCAACGTCTTTTTCGGGTCGGAAGGATTGCTACCGCTCGGC
GTGAAGCTCCCGCAAAAAATCGAGGAATACTTAAATACACTCGGCTGA

Upstream 100 bases:

>100_bases
TGATTTTACCACAATTGTTTCCCTATGTAAGCAATCCACACTATAATTGAAGTTGACCGCAAGGCGTTCCATGTAGAAAG
GAAGTGAACAGACGCAACCG

Downstream 100 bases:

>100_bases
TTAATCGTTCATCTTTTTTTCACAATTTGAACACACGTTATTCCAAGAACGTTTTTGAATATGGTATGATGACATATGAG
GTTTATTCGAAAAGGGGGAT

Product: RimK domain protein ATP-grasp

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRLRMKKVSFNMIRSAGIKTDYAKPEYYFDQLDKVRAADLVLFPEYWMVNSIIYGMNKAIFPSPATYHLGHDKIEMTRAF
KATYPHRIPKTLIYGKNPYTIERVLEEFHYPFVAKKAKSSMGQGVWLIKNDQDWLEYVENHDTFYVQEFIPNDRDLRVIV
IGEEVVGAYWRVSEAFLNNVAQGAHFSYEDIPEDVVQEVQSIANTLSINHAAFDVIVTEDGFYILEFNVFFGSEGLLPLG
VKLPQKIEEYLNTLG

Sequences:

>Translated_255_residues
MRLRMKKVSFNMIRSAGIKTDYAKPEYYFDQLDKVRAADLVLFPEYWMVNSIIYGMNKAIFPSPATYHLGHDKIEMTRAF
KATYPHRIPKTLIYGKNPYTIERVLEEFHYPFVAKKAKSSMGQGVWLIKNDQDWLEYVENHDTFYVQEFIPNDRDLRVIV
IGEEVVGAYWRVSEAFLNNVAQGAHFSYEDIPEDVVQEVQSIANTLSINHAAFDVIVTEDGFYILEFNVFFGSEGLLPLG
VKLPQKIEEYLNTLG
>Mature_255_residues
MRLRMKKVSFNMIRSAGIKTDYAKPEYYFDQLDKVRAADLVLFPEYWMVNSIIYGMNKAIFPSPATYHLGHDKIEMTRAF
KATYPHRIPKTLIYGKNPYTIERVLEEFHYPFVAKKAKSSMGQGVWLIKNDQDWLEYVENHDTFYVQEFIPNDRDLRVIV
IGEEVVGAYWRVSEAFLNNVAQGAHFSYEDIPEDVVQEVQSIANTLSINHAAFDVIVTEDGFYILEFNVFFGSEGLLPLG
VKLPQKIEEYLNTLG

Specific function: Catalyzes the ATP-dependent addition of one L-glutamate molecule to gamma-F420-2, producing the mature coenzyme alpha- F420-3 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 29544; Mature: 29544

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRMKKVSFNMIRSAGIKTDYAKPEYYFDQLDKVRAADLVLFPEYWMVNSIIYGMNKAI
CCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCC
FPSPATYHLGHDKIEMTRAFKATYPHRIPKTLIYGKNPYTIERVLEEFHYPFVAKKAKSS
CCCCCEEECCCCHHHHHHHHHHCCCCCCCHHEEECCCCHHHHHHHHHHCCCHHHHHHHHC
MGQGVWLIKNDQDWLEYVENHDTFYVQEFIPNDRDLRVIVIGEEVVGAYWRVSEAFLNNV
CCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
AQGAHFSYEDIPEDVVQEVQSIANTLSINHAAFDVIVTEDGFYILEFNVFFGSEGLLPLG
HCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEEEECCCCCEECC
VKLPQKIEEYLNTLG
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MRLRMKKVSFNMIRSAGIKTDYAKPEYYFDQLDKVRAADLVLFPEYWMVNSIIYGMNKAI
CCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCC
FPSPATYHLGHDKIEMTRAFKATYPHRIPKTLIYGKNPYTIERVLEEFHYPFVAKKAKSS
CCCCCEEECCCCHHHHHHHHHHCCCCCCCHHEEECCCCHHHHHHHHHHCCCHHHHHHHHC
MGQGVWLIKNDQDWLEYVENHDTFYVQEFIPNDRDLRVIVIGEEVVGAYWRVSEAFLNNV
CCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
AQGAHFSYEDIPEDVVQEVQSIANTLSINHAAFDVIVTEDGFYILEFNVFFGSEGLLPLG
HCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEEEECCCCCEECC
VKLPQKIEEYLNTLG
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]