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The map label for this gene is def2 [H]

Identifier: 229918497

GI number: 229918497

Start: 2751522

End: 2752061

Strand: Reverse

Name: def2 [H]

Synonym: EAT1b_2784

Alternate gene names: 229918497

Gene position: 2752061-2751522 (Counterclockwise)

Preceding gene: 229918502

Following gene: 229918496

Centisome position: 91.74

GC content: 50.56

Gene sequence:

>540_bases
ATGTTAACCATGAAAGATGTCATCCGCGAAGGAGACGAGCGGCTTCGCGCGCGCTCAACTGAAGTACCGATTCCGCCAAC
GGCAGAAGATTTGGCGTTACTCGACGAGATGGAGACGTTCCTCGTCAATAGTCAAGACCCAGAGATGAGCGAGAAATACG
AGTTGCGAGGTGGGGTCGGTATCGCCGCGCCACAACTCGGTGTGAATCGTCGTTTCTTCACAGTCTTGCTACAAGAAGAG
GAAGAGACGTTCAAACTCTCCATCTTCAATCCTAAAATCACAAGTCATTCCGTCGAGCAGACATTTTTGAATGGCGGCGA
AGGCTGTCTATCGGTCGATCGCGTGGTCAAAGGAAATGTACCTCGTTTTCGTCGAATCACGTTAGAAGGGTTCGACCGCG
ATGGGAAGCCCATTAAGCTTCGCCTGCGTGGAATGCGCGCTGTCGTATGTCAGCACGAGCTCGATCATTTAGATGGGATC
ATGTTCTATGACCGCATCAATATGTCGAACCCGTTAGAAACATACGGCGAACCGATTTAA

Upstream 100 bases:

>100_bases
CACTGTATTATTTTCATTTCACCTTCTGTACCATCCACATTCCTTCCTGTTTAAATATGATATAATCAATCATATATGTT
TGATAGAAAGGAAGTTGTGT

Downstream 100 bases:

>100_bases
CATGTTTAGCTCGAGAAAAAGGGCACTGTTGTTACTATTCCCATTTTTATTTCTTTTAATCGGTGGATGTGTGCGAATCA
ATTATGATGCGACTGTTCAC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MLTMKDVIREGDERLRARSTEVPIPPTAEDLALLDEMETFLVNSQDPEMSEKYELRGGVGIAAPQLGVNRRFFTVLLQEE
EETFKLSIFNPKITSHSVEQTFLNGGEGCLSVDRVVKGNVPRFRRITLEGFDRDGKPIKLRLRGMRAVVCQHELDHLDGI
MFYDRINMSNPLETYGEPI

Sequences:

>Translated_179_residues
MLTMKDVIREGDERLRARSTEVPIPPTAEDLALLDEMETFLVNSQDPEMSEKYELRGGVGIAAPQLGVNRRFFTVLLQEE
EETFKLSIFNPKITSHSVEQTFLNGGEGCLSVDRVVKGNVPRFRRITLEGFDRDGKPIKLRLRGMRAVVCQHELDHLDGI
MFYDRINMSNPLETYGEPI
>Mature_179_residues
MLTMKDVIREGDERLRARSTEVPIPPTAEDLALLDEMETFLVNSQDPEMSEKYELRGGVGIAAPQLGVNRRFFTVLLQEE
EETFKLSIFNPKITSHSVEQTFLNGGEGCLSVDRVVKGNVPRFRRITLEGFDRDGKPIKLRLRGMRAVVCQHELDHLDGI
MFYDRINMSNPLETYGEPI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=110, Percent_Identity=34.5454545454545, Blast_Score=72, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645728, Length=173, Percent_Identity=30.635838150289, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20398; Mature: 20398

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMKDVIREGDERLRARSTEVPIPPTAEDLALLDEMETFLVNSQDPEMSEKYELRGGVG
CCCHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCE
IAAPQLGVNRRFFTVLLQEEEETFKLSIFNPKITSHSVEQTFLNGGEGCLSVDRVVKGNV
EECCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHCCCCCCEEHHHHHCCCC
PRFRRITLEGFDRDGKPIKLRLRGMRAVVCQHELDHLDGIMFYDRINMSNPLETYGEPI
CCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHCCCCC
>Mature Secondary Structure
MLTMKDVIREGDERLRARSTEVPIPPTAEDLALLDEMETFLVNSQDPEMSEKYELRGGVG
CCCHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCE
IAAPQLGVNRRFFTVLLQEEEETFKLSIFNPKITSHSVEQTFLNGGEGCLSVDRVVKGNV
EECCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHCCCCCCEEHHHHHCCCC
PRFRRITLEGFDRDGKPIKLRLRGMRAVVCQHELDHLDGIMFYDRINMSNPLETYGEPI
CCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]