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The map label for this gene is fabI [H]

Identifier: 229918470

GI number: 229918470

Start: 2728888

End: 2729661

Strand: Direct

Name: fabI [H]

Synonym: EAT1b_2757

Alternate gene names: 229918470

Gene position: 2728888-2729661 (Clockwise)

Preceding gene: 229918468

Following gene: 229918471

Centisome position: 90.97

GC content: 50.13

Gene sequence:

>774_bases
ATGTCAATTTTTCCAACATTAACGAATAAAACGTACGTCGTCATGGGGATTGCCAACAAGCGCTCGATTGCATGGGCTAT
TGCCAAAACGCTTGATGAGGCAGGGGCGAAGCTCGTATTAACGTATGCGGCAGAACGATTCAAATCAGGTCTCGAGTCAC
TCGCAAGTGAGTTGAAGCAAGAAGCGATTCTACTTGAGTGTGATGTGACGAATGACGAAGCGATTGATTCAACATTCCGT
TCGATTCACGAACAAGCGGGCGAGATTCAAGGAATCGCGCATTCGATCGCATTTGCTGACAAAGAAGCATTGCGCGGTGA
GTTCTCAGAAATGACACGTGGTCAGTTCGCGCAGGCATTAGACATCTCAGCATATAGTTTGACGGCAGTTGTGAAAGCGG
CGAAGCCTTACTTCGCACAAGACGCCTCGGTGATCACACTCACTTACCTCGGCGGCGAGCGTGTCGTTCCGAACTACAAC
TTGATGGGTGTCGCCAAAGCGGCACTTGATTCGAGCGTCAAGTATTTGGCGAACGAATACGGTCCACATGGTGTGCGTGT
CAATGCTATCTCGGCGGGGCCGATTCGTACATTGTCAGCCAAAGGGGTTGGTGATTTCAACAGTATCCTTTCTGAGCTTG
AACAGAAGGCGCCATTGCGTCGAAACGTTTCGGCGGAAGAAGTAGCTTCGACAGGCCTCTTCCTCCTCTCGGGGATGGGA
AGTGGCGTGACCGGCGAAGTCATTCACGTAGATAGCGGATACCATATTTTATAA

Upstream 100 bases:

>100_bases
AAAAAAATCGCAATACGTAACGAGTCGGTTGACAAAACAGAAACCTTTCTGTAATCTGAGGACAGATATTATCACCAGGT
ACTAAAAAGGGGAGTTTATG

Downstream 100 bases:

>100_bases
GTTTGGGGTATGGCGCAGTGATGCGCCATTTTTGTATTACCTCGTGAATAAATCGGGTAAGGACCTATGAAGGAGGTCAG
ATCATGGGTCCCTATCAGAT

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSIFPTLTNKTYVVMGIANKRSIAWAIAKTLDEAGAKLVLTYAAERFKSGLESLASELKQEAILLECDVTNDEAIDSTFR
SIHEQAGEIQGIAHSIAFADKEALRGEFSEMTRGQFAQALDISAYSLTAVVKAAKPYFAQDASVITLTYLGGERVVPNYN
LMGVAKAALDSSVKYLANEYGPHGVRVNAISAGPIRTLSAKGVGDFNSILSELEQKAPLRRNVSAEEVASTGLFLLSGMG
SGVTGEVIHVDSGYHIL

Sequences:

>Translated_257_residues
MSIFPTLTNKTYVVMGIANKRSIAWAIAKTLDEAGAKLVLTYAAERFKSGLESLASELKQEAILLECDVTNDEAIDSTFR
SIHEQAGEIQGIAHSIAFADKEALRGEFSEMTRGQFAQALDISAYSLTAVVKAAKPYFAQDASVITLTYLGGERVVPNYN
LMGVAKAALDSSVKYLANEYGPHGVRVNAISAGPIRTLSAKGVGDFNSILSELEQKAPLRRNVSAEEVASTGLFLLSGMG
SGVTGEVIHVDSGYHIL
>Mature_256_residues
SIFPTLTNKTYVVMGIANKRSIAWAIAKTLDEAGAKLVLTYAAERFKSGLESLASELKQEAILLECDVTNDEAIDSTFRS
IHEQAGEIQGIAHSIAFADKEALRGEFSEMTRGQFAQALDISAYSLTAVVKAAKPYFAQDASVITLTYLGGERVVPNYNL
MGVAKAALDSSVKYLANEYGPHGVRVNAISAGPIRTLSAKGVGDFNSILSELEQKAPLRRNVSAEEVASTGLFLLSGMGS
GVTGEVIHVDSGYHIL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=251, Percent_Identity=44.2231075697211, Blast_Score=228, Evalue=2e-61,
Organism=Escherichia coli, GI1789378, Length=267, Percent_Identity=25.0936329588015, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=27.4131274131274, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787335, Length=243, Percent_Identity=26.7489711934156, Blast_Score=62, Evalue=3e-11,
Organism=Drosophila melanogaster, GI23397609, Length=258, Percent_Identity=28.2945736434109, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27450; Mature: 27319

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFPTLTNKTYVVMGIANKRSIAWAIAKTLDEAGAKLVLTYAAERFKSGLESLASELKQ
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHH
EAILLECDVTNDEAIDSTFRSIHEQAGEIQGIAHSIAFADKEALRGEFSEMTRGQFAQAL
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DISAYSLTAVVKAAKPYFAQDASVITLTYLGGERVVPNYNLMGVAKAALDSSVKYLANEY
CCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHC
GPHGVRVNAISAGPIRTLSAKGVGDFNSILSELEQKAPLRRNVSAEEVASTGLFLLSGMG
CCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHEEECCC
SGVTGEVIHVDSGYHIL
CCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SIFPTLTNKTYVVMGIANKRSIAWAIAKTLDEAGAKLVLTYAAERFKSGLESLASELKQ
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHH
EAILLECDVTNDEAIDSTFRSIHEQAGEIQGIAHSIAFADKEALRGEFSEMTRGQFAQAL
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DISAYSLTAVVKAAKPYFAQDASVITLTYLGGERVVPNYNLMGVAKAALDSSVKYLANEY
CCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHC
GPHGVRVNAISAGPIRTLSAKGVGDFNSILSELEQKAPLRRNVSAEEVASTGLFLLSGMG
CCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHEEECCC
SGVTGEVIHVDSGYHIL
CCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]