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The map label for this gene is trpS [H]

Identifier: 229918448

GI number: 229918448

Start: 2706672

End: 2707673

Strand: Reverse

Name: trpS [H]

Synonym: EAT1b_2735

Alternate gene names: 229918448

Gene position: 2707673-2706672 (Counterclockwise)

Preceding gene: 229918449

Following gene: 229918432

Centisome position: 90.26

GC content: 47.21

Gene sequence:

>1002_bases
ATGGCAACGATTTTTTCAGGAATCAAACCGACCGGCGTCGTGACGCTCGGGAACTACTTAGGCGCAATGAAGCAATTCGT
CGATCTTCAAGATGACAACAATGCGTATTATTGTATCGTCGATTTACATTCGATTACCGTTCAACTCGATCGTGTCGAGT
TAATGAACAACACGCGAAAGTTGGCGGCACTCTACTTGGCTTCTGGTCTCGACCCAGAAAAAGCGACGATTTTTGTTCAG
TCTGAAGTGAAGGCGCACGCTCAACTCGGTTGGATGTTAACTTGTATCGCCGGCATGGGTGAGCTCGGCCGGATGACGCA
GTATAAAGACAAGTCGCAAGGTAGTGAGAACGTTGGCGCAGGGCTGTTCGTTTACCCGACGCTCATGGCATCTGACATCT
TGTTATACAATACGGAACTCGTTCCAGTCGGAGACGACCAAAAGCAACACATCGAGTTGACTCGTGATTTGGCGGAACGC
TTTAACAAACGATATTATGAGACATTCACAATCCCAGAGCCACTCATTCAAAAAGAAGGCGCACGGATCATGTCTCTCAC
GAATCCCGTGAAGAAGATGTCGAAATCGGATACGAATGCCAAAGGATATATCTCGATGCTCGACGAGCCGAATGTCATCC
GTAAGAAGATTAAGAGTGCCGTCACAGACTCGAGTGGTGTCATCTCGTTCGACCGGGAGAACAAACCGGGGATTTCGAAC
TTGTTAGAAATTTACTCGCTTTCGACGGGGACCCCAATCGATGATCTCGTCAAACAATATGAGAACTCGAACTATGGGAC
GTTCAAACAAGATGTGGCTGAAGCGGTTGTTGCTCTACTTGAACCAATTCAAGCTCGTTATCACGAATTGATCGATTCTC
CAGAACTCGATGAAATTCTTGATGCCGGTCGTGCGAAAGCTGAAGCCGTCGCAAATAAGAATCTCGCGAAGATTGAAAAA
GCGATGGGTCTCAGTCGTAAGCGCGCCAAAGTGAAAAAATAA

Upstream 100 bases:

>100_bases
TGGCATATGATATGCAAATTTGGGTGGCACCGCGGTTATTCGTCCCGATTTATTCGGGAGGACGACTGCGGTTTTTTATA
TTCTTCGGAGGTGTATACAC

Downstream 100 bases:

>100_bases
GATTGTTGTGAAAGAACTCTCCATGTGAGAGTTCTTTTTTGTATAAAAAAATGTACATAAAAAGAGAAACAGGCGTCCCT
GTCTCTCTTCGTAAGATGAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MATIFSGIKPTGVVTLGNYLGAMKQFVDLQDDNNAYYCIVDLHSITVQLDRVELMNNTRKLAALYLASGLDPEKATIFVQ
SEVKAHAQLGWMLTCIAGMGELGRMTQYKDKSQGSENVGAGLFVYPTLMASDILLYNTELVPVGDDQKQHIELTRDLAER
FNKRYYETFTIPEPLIQKEGARIMSLTNPVKKMSKSDTNAKGYISMLDEPNVIRKKIKSAVTDSSGVISFDRENKPGISN
LLEIYSLSTGTPIDDLVKQYENSNYGTFKQDVAEAVVALLEPIQARYHELIDSPELDEILDAGRAKAEAVANKNLAKIEK
AMGLSRKRAKVKK

Sequences:

>Translated_333_residues
MATIFSGIKPTGVVTLGNYLGAMKQFVDLQDDNNAYYCIVDLHSITVQLDRVELMNNTRKLAALYLASGLDPEKATIFVQ
SEVKAHAQLGWMLTCIAGMGELGRMTQYKDKSQGSENVGAGLFVYPTLMASDILLYNTELVPVGDDQKQHIELTRDLAER
FNKRYYETFTIPEPLIQKEGARIMSLTNPVKKMSKSDTNAKGYISMLDEPNVIRKKIKSAVTDSSGVISFDRENKPGISN
LLEIYSLSTGTPIDDLVKQYENSNYGTFKQDVAEAVVALLEPIQARYHELIDSPELDEILDAGRAKAEAVANKNLAKIEK
AMGLSRKRAKVKK
>Mature_332_residues
ATIFSGIKPTGVVTLGNYLGAMKQFVDLQDDNNAYYCIVDLHSITVQLDRVELMNNTRKLAALYLASGLDPEKATIFVQS
EVKAHAQLGWMLTCIAGMGELGRMTQYKDKSQGSENVGAGLFVYPTLMASDILLYNTELVPVGDDQKQHIELTRDLAERF
NKRYYETFTIPEPLIQKEGARIMSLTNPVKKMSKSDTNAKGYISMLDEPNVIRKKIKSAVTDSSGVISFDRENKPGISNL
LEIYSLSTGTPIDDLVKQYENSNYGTFKQDVAEAVVALLEPIQARYHELIDSPELDEILDAGRAKAEAVANKNLAKIEKA
MGLSRKRAKVKK

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=324, Percent_Identity=39.1975308641975, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI41352700, Length=175, Percent_Identity=48, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1789786, Length=328, Percent_Identity=53.3536585365854, Blast_Score=349, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI71982800, Length=330, Percent_Identity=37.5757575757576, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI71982793, Length=330, Percent_Identity=37.5757575757576, Blast_Score=218, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6320474, Length=346, Percent_Identity=42.1965317919075, Blast_Score=246, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24666151, Length=324, Percent_Identity=41.6666666666667, Blast_Score=259, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 36968; Mature: 36837

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIFSGIKPTGVVTLGNYLGAMKQFVDLQDDNNAYYCIVDLHSITVQLDRVELMNNTRK
CCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEHHHHHHHHHHHH
LAALYLASGLDPEKATIFVQSEVKAHAQLGWMLTCIAGMGELGRMTQYKDKSQGSENVGA
HHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
GLFVYPTLMASDILLYNTELVPVGDDQKQHIELTRDLAERFNKRYYETFTIPEPLIQKEG
CHHHHHHHHHCCEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHCC
ARIMSLTNPVKKMSKSDTNAKGYISMLDEPNVIRKKIKSAVTDSSGVISFDRENKPGISN
CHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
LLEIYSLSTGTPIDDLVKQYENSNYGTFKQDVAEAVVALLEPIQARYHELIDSPELDEIL
HHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DAGRAKAEAVANKNLAKIEKAMGLSRKRAKVKK
HCCCHHHHHHHCCHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
ATIFSGIKPTGVVTLGNYLGAMKQFVDLQDDNNAYYCIVDLHSITVQLDRVELMNNTRK
CCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEHHHHHHHHHHHH
LAALYLASGLDPEKATIFVQSEVKAHAQLGWMLTCIAGMGELGRMTQYKDKSQGSENVGA
HHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
GLFVYPTLMASDILLYNTELVPVGDDQKQHIELTRDLAERFNKRYYETFTIPEPLIQKEG
CHHHHHHHHHCCEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHCC
ARIMSLTNPVKKMSKSDTNAKGYISMLDEPNVIRKKIKSAVTDSSGVISFDRENKPGISN
CHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
LLEIYSLSTGTPIDDLVKQYENSNYGTFKQDVAEAVVALLEPIQARYHELIDSPELDEIL
HHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DAGRAKAEAVANKNLAKIEKAMGLSRKRAKVKK
HCCCHHHHHHHCCHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]