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The map label for this gene is iscS1 [H]

Identifier: 229918429

GI number: 229918429

Start: 2686366

End: 2687502

Strand: Direct

Name: iscS1 [H]

Synonym: EAT1b_2716

Alternate gene names: 229918429

Gene position: 2686366-2687502 (Clockwise)

Preceding gene: 229918428

Following gene: 229918430

Centisome position: 89.55

GC content: 52.15

Gene sequence:

>1137_bases
ATGAACTATTTTGATCATGCTGCGACGACGCCGATGCGACCGGAAGTCCTCGAAGCGATGACACCGTACTTTTTAGAAAC
ATACGGGAACGCATCGAGCATCCACGGAATCGGGCGTAACGCTCGGGCGGCGCTCGATTCGTCACGTCGCGTCCTCGCGA
AGTGGATTGGTGCTTCACCGAACGAGATTGTATTCACGTCAGGTGGGACGGAGTCGGATAATTATGCCTTGTTCGGTGCG
GCATATGCCCATCAGGAAAAAGGCAAACATATCATTACGACAAAAGTCGAGCACCACGCCGTGCTTCATGCGATGGAACA
GCTCGAGCGTGAGGGCTTCGACGTCACATATCTCGATGTCGATGCTTCGGGAGCCGTCACTGTCGACGCCGTTCGTTCAG
CCTTACGGGATGACACGATTCTTGTATCGATCATGTATGGCAATAACGAAGTCGGGACGCGCCAGCCGATTCACGAAATC
GGGGAGTTGTTACAAGATCACCAGGCTCTCTTCCATACCGATGCGGTACAAGCACTCGGTACAGAAGAAATTGATGTGAA
GGCGATTCATGTCGATTTATTGTCGGCGTCCGCACATAAAGTGAATGGGCCGAAAGGTATCGGTTTTCTTTTCATGAAAA
CAGGTGTAAAATTAGCGTCACGTTCTTTTGGTGGACAACAAGAACGTAAACGTCGAGCCGGCACGGAAAATGTTCCGGGT
GCCGTCGGATTTCAAAAAGCAGCTGAACTCGTCATGGCAGAGCGTGAGACCCGAGTGACTCTTTATGAATCGCTCGATCT
AGCACTACGTCAGCGCCTGGACGAATTAGGCGTGTCTTATGAGGTGAACGGGTCAAATCGTTTACCTCACATCGTGAACT
TATACCTTCCTGGAATTGACCTTGAACCCTTCCTCATCATGATGGATATGCGGGGCATGGCGATTTCAAGCGGAAGTGCC
TGCACGGCAGGGTCGGTCGAACCGTCCCACGTATTGTCGGCGATGTACGGGGATTCAGAGCGGACGCGTCAGTCTGTTCG
AATCAGTTTTGGCCACGGCAACTCGGTCGAGGACGCGAAACGGCTCGCGGAGCGACTACAAGAAGTCGTCTCGACATTTA
TGAACGGAGTGAGATAG

Upstream 100 bases:

>100_bases
AAGCCATCGATACCGTCCTTGACGAAACGACACTCGCGGACCTCGCTGAAGAAGAGTCAGACGGCTACATGTTTTATATT
TAAGAACGAGGTGACCTTAG

Downstream 100 bases:

>100_bases
ATGAATTTACGCACGAAAGCCCCTGAAGAGACGACGGTCGTCGTCGGCATGTCAGGTGGCGTCGATTCATCGGTGACAGC
CCATGTGTTAAAAGAGCAAG

Product: Cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNYFDHAATTPMRPEVLEAMTPYFLETYGNASSIHGIGRNARAALDSSRRVLAKWIGASPNEIVFTSGGTESDNYALFGA
AYAHQEKGKHIITTKVEHHAVLHAMEQLEREGFDVTYLDVDASGAVTVDAVRSALRDDTILVSIMYGNNEVGTRQPIHEI
GELLQDHQALFHTDAVQALGTEEIDVKAIHVDLLSASAHKVNGPKGIGFLFMKTGVKLASRSFGGQQERKRRAGTENVPG
AVGFQKAAELVMAERETRVTLYESLDLALRQRLDELGVSYEVNGSNRLPHIVNLYLPGIDLEPFLIMMDMRGMAISSGSA
CTAGSVEPSHVLSAMYGDSERTRQSVRISFGHGNSVEDAKRLAERLQEVVSTFMNGVR

Sequences:

>Translated_378_residues
MNYFDHAATTPMRPEVLEAMTPYFLETYGNASSIHGIGRNARAALDSSRRVLAKWIGASPNEIVFTSGGTESDNYALFGA
AYAHQEKGKHIITTKVEHHAVLHAMEQLEREGFDVTYLDVDASGAVTVDAVRSALRDDTILVSIMYGNNEVGTRQPIHEI
GELLQDHQALFHTDAVQALGTEEIDVKAIHVDLLSASAHKVNGPKGIGFLFMKTGVKLASRSFGGQQERKRRAGTENVPG
AVGFQKAAELVMAERETRVTLYESLDLALRQRLDELGVSYEVNGSNRLPHIVNLYLPGIDLEPFLIMMDMRGMAISSGSA
CTAGSVEPSHVLSAMYGDSERTRQSVRISFGHGNSVEDAKRLAERLQEVVSTFMNGVR
>Mature_378_residues
MNYFDHAATTPMRPEVLEAMTPYFLETYGNASSIHGIGRNARAALDSSRRVLAKWIGASPNEIVFTSGGTESDNYALFGA
AYAHQEKGKHIITTKVEHHAVLHAMEQLEREGFDVTYLDVDASGAVTVDAVRSALRDDTILVSIMYGNNEVGTRQPIHEI
GELLQDHQALFHTDAVQALGTEEIDVKAIHVDLLSASAHKVNGPKGIGFLFMKTGVKLASRSFGGQQERKRRAGTENVPG
AVGFQKAAELVMAERETRVTLYESLDLALRQRLDELGVSYEVNGSNRLPHIVNLYLPGIDLEPFLIMMDMRGMAISSGSA
CTAGSVEPSHVLSAMYGDSERTRQSVRISFGHGNSVEDAKRLAERLQEVVSTFMNGVR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=369, Percent_Identity=39.8373983739837, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=34.9514563106796, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI48994898, Length=363, Percent_Identity=42.4242424242424, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1787970, Length=402, Percent_Identity=25.6218905472637, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789175, Length=269, Percent_Identity=26.0223048327138, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25143064, Length=376, Percent_Identity=40.1595744680851, Blast_Score=254, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI17533177, Length=332, Percent_Identity=31.0240963855422, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6319831, Length=376, Percent_Identity=40.1595744680851, Blast_Score=254, Evalue=1e-68,
Organism=Drosophila melanogaster, GI20129463, Length=380, Percent_Identity=40.5263157894737, Blast_Score=267, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41345; Mature: 41345

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYFDHAATTPMRPEVLEAMTPYFLETYGNASSIHGIGRNARAALDSSRRVLAKWIGASP
CCCCCCCCCCCCCHHHHHHHCHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
NEIVFTSGGTESDNYALFGAAYAHQEKGKHIITTKVEHHAVLHAMEQLEREGFDVTYLDV
CEEEEECCCCCCCCEEEEEEHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEE
DASGAVTVDAVRSALRDDTILVSIMYGNNEVGTRQPIHEIGELLQDHQALFHTDAVQALG
CCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
TEEIDVKAIHVDLLSASAHKVNGPKGIGFLFMKTGVKLASRSFGGQQERKRRAGTENVPG
CCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEHHCHHHHHHCCCCHHHHHHHCCCCCCCC
AVGFQKAAELVMAERETRVTLYESLDLALRQRLDELGVSYEVNGSNRLPHIVNLYLPGID
CCCHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCC
LEPFLIMMDMRGMAISSGSACTAGSVEPSHVLSAMYGDSERTRQSVRISFGHGNSVEDAK
CCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHCCCHHCCEEEEEEECCCCCHHHHH
RLAERLQEVVSTFMNGVR
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYFDHAATTPMRPEVLEAMTPYFLETYGNASSIHGIGRNARAALDSSRRVLAKWIGASP
CCCCCCCCCCCCCHHHHHHHCHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
NEIVFTSGGTESDNYALFGAAYAHQEKGKHIITTKVEHHAVLHAMEQLEREGFDVTYLDV
CEEEEECCCCCCCCEEEEEEHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEE
DASGAVTVDAVRSALRDDTILVSIMYGNNEVGTRQPIHEIGELLQDHQALFHTDAVQALG
CCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
TEEIDVKAIHVDLLSASAHKVNGPKGIGFLFMKTGVKLASRSFGGQQERKRRAGTENVPG
CCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEHHCHHHHHHCCCCHHHHHHHCCCCCCCC
AVGFQKAAELVMAERETRVTLYESLDLALRQRLDELGVSYEVNGSNRLPHIVNLYLPGID
CCCHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCC
LEPFLIMMDMRGMAISSGSACTAGSVEPSHVLSAMYGDSERTRQSVRISFGHGNSVEDAK
CCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHCCCHHCCEEEEEEECCCCCHHHHH
RLAERLQEVVSTFMNGVR
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]