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The map label for this gene is nadB [H]

Identifier: 229918403

GI number: 229918403

Start: 2654434

End: 2655867

Strand: Direct

Name: nadB [H]

Synonym: EAT1b_2688

Alternate gene names: 229918403

Gene position: 2654434-2655867 (Clockwise)

Preceding gene: 229918398

Following gene: 229918404

Centisome position: 88.48

GC content: 52.3

Gene sequence:

>1434_bases
GTGTCTTTACACATAGAGAGAACTGACATTTTGATTATCGGAACGGGACTTGCCGCCGTCGCAACGGCTTTGCATCTAGC
AAAATCATTCTCCGTTCTTCTCGTCTCAAAAGGTGAGATGACAGAGAGCAACTCCGATTTGGCACAAGGCGGGATTGCGA
GCGCGTATCTTGAGAAATCGAGTACGGCCCATAAAGAAGACACGATGCTCGCCTCTAAAAACACATCGTGCGCTGAGCGC
GTCGACATTCTCGTAGAAGAAGGTCGTCAAGCGATTGAAGAAATGGAACGATTCGGTGTCCGGTTCGACCGTGAAGAGGG
CGGCTATGCCCTCGGACGTGAAGGCGCGCATCGATTGAATCGAATCTTTCATGTCGGTGGCGACGAGACGGGACGTCATG
TCATGAGGCAATTGCGTCAGTCGTTACCCGATAACGTGAACGTATTGGAACGGGTGCTCGTGCACGATCTCATCACGAGA
GACGGCAAGGTTGTCGGCGCGATGGCGTTTCGGGATGAAGTGCCCATTCGAATTGAAGCCGGCGCTGTCGTGTTGGCGAC
AGGTGGCATTGGTGGGCTGATTGATTGGACTTCAAACGCGCGTACCGTGACCGGTGACGGTCTCGTTCTGGCAAATCGAT
GTGGGGCCAAGCTGACTCATTTGCAGCGGATCCAATTTCATCCCACCTTACTGGCGACCGACGAGCCTCATCTCGTGACC
GAGGCACTCCGGGGAGCCGGAGCGACGCTCGTTGATGAAGAGGGAAACCATGTGATGGCGCATCATCCGCTCGGAAGTCT
CGCTCCTCGTGATGACGTGGCGCGGATACTGACGGAACGAATGGGAACGGTGTATCTCGATACGACAAACGTGACACGGC
TTCGTGAACGGTTTCCAAAACTTGCGTTGACATGTGACAAACTTAGGATCGATCCTCGCCGCATTCCGGTACGTCCAGGC
CTCCATTTTCATATGGGTGGAATCGAAGTGGACGACATGGGACGGACAGACGTTGACGGATTATATGCCGTCGGTGAAGT
GGCGGATGCGGGCATACATGGGATGAATCGTTTGGCCTCGAACTCACTTTTGGAATGTTGGGTGTTTGGAAAACGAGTCG
CGCGGGCGGTGCGATTGAATCAGACGACCACGGCGACGAAGGAAACGTTGGTGTACGACATCTCACCTGTGGCATTTCAA
GCGTTACGTGCTCAAATTGGAAGATGGTTGACGATTTCACCAAGTCTTGATGAATTGAAAGGTTTCTTACAAAATACCCA
AACGTTGACCATGACGAAACACGTGACAAGACAATTAAGTGAACAAACTTTACAACTCGATGCGGCGCGTCTTCTCGCGC
AAAGCATCCTCGAAATGGAGCGGAATGATGAATACGATATACCTACGCGAACAGCTACGTCAGTTCTTGATTGA

Upstream 100 bases:

>100_bases
TTCATCATGGACTCCCCTTTTCTCATGTTGTCTTCTACACAAAATTATGCGAAACTAGTTTACAAGTCAACATTACATAT
GAAAAACAGGTGATGTACAA

Downstream 100 bases:

>100_bases
AGATATCGGGTATGGGGACCTATCGAGTGCGCTGATTGATGGACAGGACGAGTCGGTCGCAACGATTGTCGCCAAAGAAC
AGGGCATTTTCTGTGGGAAA

Product: fumarate reductase/succinate dehydrogenase flavoprotein domain protein

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSLHIERTDILIIGTGLAAVATALHLAKSFSVLLVSKGEMTESNSDLAQGGIASAYLEKSSTAHKEDTMLASKNTSCAER
VDILVEEGRQAIEEMERFGVRFDREEGGYALGREGAHRLNRIFHVGGDETGRHVMRQLRQSLPDNVNVLERVLVHDLITR
DGKVVGAMAFRDEVPIRIEAGAVVLATGGIGGLIDWTSNARTVTGDGLVLANRCGAKLTHLQRIQFHPTLLATDEPHLVT
EALRGAGATLVDEEGNHVMAHHPLGSLAPRDDVARILTERMGTVYLDTTNVTRLRERFPKLALTCDKLRIDPRRIPVRPG
LHFHMGGIEVDDMGRTDVDGLYAVGEVADAGIHGMNRLASNSLLECWVFGKRVARAVRLNQTTTATKETLVYDISPVAFQ
ALRAQIGRWLTISPSLDELKGFLQNTQTLTMTKHVTRQLSEQTLQLDAARLLAQSILEMERNDEYDIPTRTATSVLD

Sequences:

>Translated_477_residues
MSLHIERTDILIIGTGLAAVATALHLAKSFSVLLVSKGEMTESNSDLAQGGIASAYLEKSSTAHKEDTMLASKNTSCAER
VDILVEEGRQAIEEMERFGVRFDREEGGYALGREGAHRLNRIFHVGGDETGRHVMRQLRQSLPDNVNVLERVLVHDLITR
DGKVVGAMAFRDEVPIRIEAGAVVLATGGIGGLIDWTSNARTVTGDGLVLANRCGAKLTHLQRIQFHPTLLATDEPHLVT
EALRGAGATLVDEEGNHVMAHHPLGSLAPRDDVARILTERMGTVYLDTTNVTRLRERFPKLALTCDKLRIDPRRIPVRPG
LHFHMGGIEVDDMGRTDVDGLYAVGEVADAGIHGMNRLASNSLLECWVFGKRVARAVRLNQTTTATKETLVYDISPVAFQ
ALRAQIGRWLTISPSLDELKGFLQNTQTLTMTKHVTRQLSEQTLQLDAARLLAQSILEMERNDEYDIPTRTATSVLD
>Mature_476_residues
SLHIERTDILIIGTGLAAVATALHLAKSFSVLLVSKGEMTESNSDLAQGGIASAYLEKSSTAHKEDTMLASKNTSCAERV
DILVEEGRQAIEEMERFGVRFDREEGGYALGREGAHRLNRIFHVGGDETGRHVMRQLRQSLPDNVNVLERVLVHDLITRD
GKVVGAMAFRDEVPIRIEAGAVVLATGGIGGLIDWTSNARTVTGDGLVLANRCGAKLTHLQRIQFHPTLLATDEPHLVTE
ALRGAGATLVDEEGNHVMAHHPLGSLAPRDDVARILTERMGTVYLDTTNVTRLRERFPKLALTCDKLRIDPRRIPVRPGL
HFHMGGIEVDDMGRTDVDGLYAVGEVADAGIHGMNRLASNSLLECWVFGKRVARAVRLNQTTTATKETLVYDISPVAFQA
LRAQIGRWLTISPSLDELKGFLQNTQTLTMTKHVTRQLSEQTLQLDAARLLAQSILEMERNDEYDIPTRTATSVLD

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=392, Percent_Identity=31.8877551020408, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788928, Length=399, Percent_Identity=39.0977443609023, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790597, Length=377, Percent_Identity=32.8912466843501, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1786942, Length=418, Percent_Identity=33.0143540669856, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17505833, Length=391, Percent_Identity=29.923273657289, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17550100, Length=394, Percent_Identity=30.7106598984772, Blast_Score=131, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6322701, Length=417, Percent_Identity=30.9352517985612, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322416, Length=391, Percent_Identity=30.4347826086957, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320788, Length=466, Percent_Identity=25.3218884120172, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322511, Length=470, Percent_Identity=22.9787234042553, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137288, Length=397, Percent_Identity=31.7380352644836, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24655642, Length=397, Percent_Identity=31.7380352644836, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24655647, Length=397, Percent_Identity=31.7380352644836, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24663005, Length=427, Percent_Identity=29.2740046838407, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 52394; Mature: 52262

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHIERTDILIIGTGLAAVATALHLAKSFSVLLVSKGEMTESNSDLAQGGIASAYLEKS
CCEEEEECCEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHCCHHHHHHHHC
STAHKEDTMLASKNTSCAERVDILVEEGRQAIEEMERFGVRFDREEGGYALGREGAHRLN
CCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEECCHHHHHHHH
RIFHVGGDETGRHVMRQLRQSLPDNVNVLERVLVHDLITRDGKVVGAMAFRDEVPIRIEA
HEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEC
GAVVLATGGIGGLIDWTSNARTVTGDGLVLANRCGAKLTHLQRIQFHPTLLATDEPHLVT
CEEEEEECCCCCEEECCCCCEEEECCCEEEECCCCCHHHHHHHEEECCEEEECCCCHHHH
EALRGAGATLVDEEGNHVMAHHPLGSLAPRDDVARILTERMGTVYLDTTNVTRLRERFPK
HHHHCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHCCH
LALTCDKLRIDPRRIPVRPGLHFHMGGIEVDDMGRTDVDGLYAVGEVADAGIHGMNRLAS
HEEEHHHHEECCCCCCCCCCCEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NSLLECWVFGKRVARAVRLNQTTTATKETLVYDISPVAFQALRAQIGRWLTISPSLDELK
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEECCHHHHHHHHHHHCCEEEECCCHHHHH
GFLQNTQTLTMTKHVTRQLSEQTLQLDAARLLAQSILEMERNDEYDIPTRTATSVLD
HHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SLHIERTDILIIGTGLAAVATALHLAKSFSVLLVSKGEMTESNSDLAQGGIASAYLEKS
CEEEEECCEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHCCHHHHHHHHC
STAHKEDTMLASKNTSCAERVDILVEEGRQAIEEMERFGVRFDREEGGYALGREGAHRLN
CCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEECCHHHHHHHH
RIFHVGGDETGRHVMRQLRQSLPDNVNVLERVLVHDLITRDGKVVGAMAFRDEVPIRIEA
HEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEC
GAVVLATGGIGGLIDWTSNARTVTGDGLVLANRCGAKLTHLQRIQFHPTLLATDEPHLVT
CEEEEEECCCCCEEECCCCCEEEECCCEEEECCCCCHHHHHHHEEECCEEEECCCCHHHH
EALRGAGATLVDEEGNHVMAHHPLGSLAPRDDVARILTERMGTVYLDTTNVTRLRERFPK
HHHHCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHCCH
LALTCDKLRIDPRRIPVRPGLHFHMGGIEVDDMGRTDVDGLYAVGEVADAGIHGMNRLAS
HEEEHHHHEECCCCCCCCCCCEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NSLLECWVFGKRVARAVRLNQTTTATKETLVYDISPVAFQALRAQIGRWLTISPSLDELK
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEECCHHHHHHHHHHHCCEEEECCCHHHHH
GFLQNTQTLTMTKHVTRQLSEQTLQLDAARLLAQSILEMERNDEYDIPTRTATSVLD
HHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]