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The map label for this gene is rne [H]

Identifier: 229918392

GI number: 229918392

Start: 2645547

End: 2646872

Strand: Direct

Name: rne [H]

Synonym: EAT1b_2677

Alternate gene names: 229918392

Gene position: 2645547-2646872 (Clockwise)

Preceding gene: 229918391

Following gene: 229918393

Centisome position: 88.19

GC content: 53.62

Gene sequence:

>1326_bases
ATGAAGCTAATCTACGAACGAACACCTTCCATTGAACGGGCGATGCTCGTGGACGGCGAACGATTACTCGAAGTCGAGGA
ACGGTTTGTCGACCAACTGTCTGTCGGGACCATCGTCGAAGCCGCAATTGAACGGATCCATCCGTCGCTCGGGGCAGCCT
TTTTGACGGACGGAGAGACGACCTATTTTCTCCCGATCTCAGAGACGCTTCGTGCGCTCGCCTCGTATCCGGAAGTACCG
GCCATCGGGCAAGCGGCAGTCGTCGGGGAAAAGCGACTCGTCCAATTGACGAAAGAAGGAATCAACGGTAAGCATCATAA
AGTGACAGAGAACATTCGGTTCGGTGGGCGATATCTTGTGTATTTCCCATTTGGACGTCGGCAACTGTTCAGCCGGAAAC
TCGACCTCGTCACGCAGGACCGCCTTTCAAAGGCGCTCGACTTAACGGAAGAGGAAGGGGTCTTGTTTCGGACGGAAGCC
GGACAAGTGGCGCCGGAAGCGTTACAGGAAGAACTGAACCGATTGCGTCACCGTTCAATCGTGGTGATTGAGCAACGGTT
CGGGCAAGACGAGCTACTATGCGAACAATTCGTGAGACGTCATGCAGTCGAGTCCGTGCTGTTGAATGACAAAGAAGGGC
AATCGCGACTCGAACGATTGGATGTCCCGGTGAAACGTCATATCGGAAAAGGGCGAATGCCTGAAGCGAAACATCTCGAT
GGAGCCATCGAGAAACTGGAGAGTCGTGCCGTCTGGTTGGATGGTGGGGCGTTCGTCTTGATCGAACAAGTCGAGACGAT
GACCGTCATCGACGTGAACAGTGGCAAAAATGTCGCCGTCAAAGACAAGGGACGTGCGGCCGATACCATCAATGAAGCCG
CGCTCTATTCAATCATGGAGCAGTTGCGCCTGCGAAACATCGGCGGCATGATCGTCATCGATTTCTTACGAGGTTCAAAA
GAAGGACAGACCCGTCTCCTTAAAAAGATGAAAGAACTCGGGGCGCGTGACCCACGACGGGTCGAAGTGTACGGCTTCAC
ACGAATGGGGCTGTTCGAGCTGTCGCGGGAACGGACCGGTCGCTCGATTCAAGACCGGATGTTTCATGCCGGCAAGCCTT
CAAAATTGGCCATCTTCTCACAGTTTGAACGGTCGCTCCTTGATTTGAATGATCAGTACGAAGCGGTCGTGCTCTCACTC
CCCAAGAAGTGGCATGACGCTTCCTGGCACGAATTTGACATCGAGGTGCATCTCGTCGAAGGGGAGCCGGAGTTCCTCTT
TTATGGGACGTCCGAAGAATGTCAGAATTTCTTATCTCGTCGTTGA

Upstream 100 bases:

>100_bases
GTCGGCTTCTGGGCACGACTCATGATGAAATTTGGAATGAAAAAAGCGTAAAGGCAAAGGCAACGGAAGTTGCCTTTTTC
TGTCGAAAGGAGTGATGCAG

Downstream 100 bases:

>100_bases
CAATGATTAATTGAGAATGGTATTATCTATAACGTTAGTTCGTTTGGAAGCACCCAAATGGCTACAACCGCACAGAACGG
GTTTTTAAAGGTTTTGCCCT

Product: ribonuclease G and E-like protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKLIYERTPSIERAMLVDGERLLEVEERFVDQLSVGTIVEAAIERIHPSLGAAFLTDGETTYFLPISETLRALASYPEVP
AIGQAAVVGEKRLVQLTKEGINGKHHKVTENIRFGGRYLVYFPFGRRQLFSRKLDLVTQDRLSKALDLTEEEGVLFRTEA
GQVAPEALQEELNRLRHRSIVVIEQRFGQDELLCEQFVRRHAVESVLLNDKEGQSRLERLDVPVKRHIGKGRMPEAKHLD
GAIEKLESRAVWLDGGAFVLIEQVETMTVIDVNSGKNVAVKDKGRAADTINEAALYSIMEQLRLRNIGGMIVIDFLRGSK
EGQTRLLKKMKELGARDPRRVEVYGFTRMGLFELSRERTGRSIQDRMFHAGKPSKLAIFSQFERSLLDLNDQYEAVVLSL
PKKWHDASWHEFDIEVHLVEGEPEFLFYGTSEECQNFLSRR

Sequences:

>Translated_441_residues
MKLIYERTPSIERAMLVDGERLLEVEERFVDQLSVGTIVEAAIERIHPSLGAAFLTDGETTYFLPISETLRALASYPEVP
AIGQAAVVGEKRLVQLTKEGINGKHHKVTENIRFGGRYLVYFPFGRRQLFSRKLDLVTQDRLSKALDLTEEEGVLFRTEA
GQVAPEALQEELNRLRHRSIVVIEQRFGQDELLCEQFVRRHAVESVLLNDKEGQSRLERLDVPVKRHIGKGRMPEAKHLD
GAIEKLESRAVWLDGGAFVLIEQVETMTVIDVNSGKNVAVKDKGRAADTINEAALYSIMEQLRLRNIGGMIVIDFLRGSK
EGQTRLLKKMKELGARDPRRVEVYGFTRMGLFELSRERTGRSIQDRMFHAGKPSKLAIFSQFERSLLDLNDQYEAVVLSL
PKKWHDASWHEFDIEVHLVEGEPEFLFYGTSEECQNFLSRR
>Mature_441_residues
MKLIYERTPSIERAMLVDGERLLEVEERFVDQLSVGTIVEAAIERIHPSLGAAFLTDGETTYFLPISETLRALASYPEVP
AIGQAAVVGEKRLVQLTKEGINGKHHKVTENIRFGGRYLVYFPFGRRQLFSRKLDLVTQDRLSKALDLTEEEGVLFRTEA
GQVAPEALQEELNRLRHRSIVVIEQRFGQDELLCEQFVRRHAVESVLLNDKEGQSRLERLDVPVKRHIGKGRMPEAKHLD
GAIEKLESRAVWLDGGAFVLIEQVETMTVIDVNSGKNVAVKDKGRAADTINEAALYSIMEQLRLRNIGGMIVIDFLRGSK
EGQTRLLKKMKELGARDPRRVEVYGFTRMGLFELSRERTGRSIQDRMFHAGKPSKLAIFSQFERSLLDLNDQYEAVVLSL
PKKWHDASWHEFDIEVHLVEGEPEFLFYGTSEECQNFLSRR

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=389, Percent_Identity=30.3341902313625, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082235, Length=398, Percent_Identity=27.1356783919598, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 50318; Mature: 50318

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIYERTPSIERAMLVDGERLLEVEERFVDQLSVGTIVEAAIERIHPSLGAAFLTDGET
CCEEECCCCCHHHHHECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCEEEEECCCE
TYFLPISETLRALASYPEVPAIGQAAVVGEKRLVQLTKEGINGKHHKVTENIRFGGRYLV
EEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCEECCCEEEE
YFPFGRRQLFSRKLDLVTQDRLSKALDLTEEEGVLFRTEAGQVAPEALQEELNRLRHRSI
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCE
VVIEQRFGQDELLCEQFVRRHAVESVLLNDKEGQSRLERLDVPVKRHIGKGRMPEAKHLD
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHCCCCCCCCHHHHH
GAIEKLESRAVWLDGGAFVLIEQVETMTVIDVNSGKNVAVKDKGRAADTINEAALYSIME
HHHHHHHCCEEEECCCEEEEEEECCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
QLRLRNIGGMIVIDFLRGSKEGQTRLLKKMKELGARDPRRVEVYGFTRMGLFELSRERTG
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHCC
RSIQDRMFHAGKPSKLAIFSQFERSLLDLNDQYEAVVLSLPKKWHDASWHEFDIEVHLVE
CCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCCCCCEEEEEEEEEEC
GEPEFLFYGTSEECQNFLSRR
CCCCEEEECCHHHHHHHHHCC
>Mature Secondary Structure
MKLIYERTPSIERAMLVDGERLLEVEERFVDQLSVGTIVEAAIERIHPSLGAAFLTDGET
CCEEECCCCCHHHHHECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCEEEEECCCE
TYFLPISETLRALASYPEVPAIGQAAVVGEKRLVQLTKEGINGKHHKVTENIRFGGRYLV
EEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCEECCCEEEE
YFPFGRRQLFSRKLDLVTQDRLSKALDLTEEEGVLFRTEAGQVAPEALQEELNRLRHRSI
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCE
VVIEQRFGQDELLCEQFVRRHAVESVLLNDKEGQSRLERLDVPVKRHIGKGRMPEAKHLD
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHCCCCCCCCHHHHH
GAIEKLESRAVWLDGGAFVLIEQVETMTVIDVNSGKNVAVKDKGRAADTINEAALYSIME
HHHHHHHCCEEEECCCEEEEEEECCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
QLRLRNIGGMIVIDFLRGSKEGQTRLLKKMKELGARDPRRVEVYGFTRMGLFELSRERTG
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHCC
RSIQDRMFHAGKPSKLAIFSQFERSLLDLNDQYEAVVLSLPKKWHDASWHEFDIEVHLVE
CCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCCCCCEEEEEEEEEEC
GEPEFLFYGTSEECQNFLSRR
CCCCEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]