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The map label for this gene is mrpA [H]

Identifier: 229918331

GI number: 229918331

Start: 2582839

End: 2585280

Strand: Direct

Name: mrpA [H]

Synonym: EAT1b_2616

Alternate gene names: 229918331

Gene position: 2582839-2585280 (Clockwise)

Preceding gene: 229918330

Following gene: 229918332

Centisome position: 86.1

GC content: 51.8

Gene sequence:

>2442_bases
TTGTCGCTGTTACATTTAGCTATTTTGTTGCCGCTTCTTGCGGCACCATTCATCCCGCTTTTGTTCCGAGGGATGAAACA
AATTCATACAGGCTGGTTCGTCTTGATCGTTCCGGTCGTGCTCGTCACCTACTTCGCCGGATTCATTCCGATCACGAGTG
CAGGGGACGTCGTGACAGAGACGATTGCGTGGATGCCGTCCCTCGGGATCAACTTCACGGCATACGTCGATGGACTCAGT
CTCTTGTTCGCCCTTCTCATCACGGGGATCGGCGCACTGGTCGTCCTCTATTCGATTTACTATTTGAATAAGGACAAAGA
GTCGCTCGGGCATTTTTACGTGTATCTCTTGATGTTCATGAGTGCGATGCTCGGGGTCGTTCTCTCGGACAACATGATTG
TCATGTATATGTTCTGGGAGCTCACATCGATTTCATCATTCCTATTGATCGGGTACTGGTATGAGAAAGAACGTTCCCGC
TATGGTGCGCAGAAGTCGATGCTTCTCACCGTGTTCGGTGGATTGTCGATGCTCGGGGGGATCGTCTTACTCGAATCGAT
GGTCGGCTCGTACAGCATTCGCGATATGGTTGCGAACTCTGGCGTCATCGCCGAGAGCCCATTCTTCCTTGTCGCGATGG
TTCTCATCTTGCTCGGGGCGTTCACGAAGTCAGCGCAGTTCCCGTTCCACATCTGGTTGCCTGACGCGATGGAAGCACCG
ACTCCGGTCAGTGCGTACCTCCACTCAGCAACGATGGTCAAGGCGGGTCTCTACCTCGTGGCCCGCATGAGCCCGGTGTT
CCAAGAGTCATCCGTCTGGCTATGGACGATTGCCGGAGTCGGGATGTTCACGATGTTCTGGGGCTCGTTCAACGCTGTCA
AACAGACCGACCTGAAGGGGATTTTAGCGTTCTCGACGGTCAGTCAGCTCGGTTTGATCATGTCACTCCTCGGTCTCGGG
GCGGCGGCGCTCCACTATGACGGTATGGATGACAATATCTTTATGGTCGCAACGATTGCGGCAATCTTCCATTTGATCAA
CCATGCGACGTTTAAAGGTTCGCTCTTCATGATGGTCGGAATTGTCGACCACGAGACGGGCACGCGTGACATTCGGAAGC
TTGGCGGTCTGATGACTGTCATGCCGATTTCATTCACGGTCGCTGTCATCGGTTCGTTATCGATGGCCGGTCTTCCGCCA
TTCAACGGATTCTTGAGTAAAGAGATGTTCTTGAAGGCGACGACGAAAGTCTTCTCGGCCGACTTCTTTGCGCTTGATAC
GATTGGGATTTTGCTCCCGGTCATCGCGTTCGTCGGAAGTGTGTTCACGTTCATCTACAGCATCATGCTGTTCACACGGA
CGTTCATGGGCAAGGCAAAGTTCGATCAATTGCCGAAACAACCGCATGAGGCGCCGATTGGCATGTTGATTTCGCCGGTC
ATTCTCGCGCTTCTCGTTGTCGGTCTCGGGTTCTTCCCGAACGTGTTGTCGAACTCGCTCATCAAACCAGCCGTCGAGTC
GATCTATCCGACACTCGTCGCGCAAGGGCAAGATGTTAAAGTAGATATTTACTTCTGGCACGGTTTGACGCCTGAACTGT
ATATGACAATCGGGGTGATCCTTCTCGGTTCATTGCTGTTCTGGCAAATGGGTAAATGGATGGGTATCTACAACCGCATC
CCACATCGTTTGACGCTCAATGGGATGTACGACGGTACGCTCGTCTGGTCGAACAAGGCGGCTTATCGCTTTAAAGACAA
CTACATGACTGGATTCATCCGGTCGTACTTGATCTATATTTTCGTCTTCCTCTCAGGTATGATGCTCTTCAGCATCTGGT
GGTTCGACATGTACCGCTTCTCGTTTGAGAACCTGGCTGAGATTGCACCGTATGAATATGTGCTCGGAGCTGTCGTCATG
CTAGCGGCGCTCGCGATTCTCTTCATGAAGTCGCGTCTCCCGTCCATCATCCTACTTGGGGTCGTTGGATACGGCGTGGC
ACTGTTCTTCGTCTTCTTCGGTGCACCGGACTTGGCGCTTACGCAGCTCGTCATCGAGACGGTCTCGGTCGCATTGTTCT
TGCTCGTCTTCTATCACATGCCGGACATCAGCAAGAAGGAAGTTCGGATGCGTTTCAAAGTCGGGAATGCCATCGTGGCT
GTTCTCGTCGGGACGACCGTGACGCTTCTCAGCTTCATGGCGCTCACAAATAAATCGCTCACCTCGATCTCAGAGTATTA
CAAAGAGAACGTGTATGAGCTTGCAGCAGGTAAAAACATGGTCAACGTCGTTCTCGTCGACTTCCGTGGATTTGATACCT
TGTTTGAAATCGTCGTTCTCGGGATTGCAGCTTTCGGGATCTACACGATGATTAAATTTAGAACAACACGACGTACGAAA
GGAGAAGGTGAACATGAAGCAAAACAGCAGACGCGTCAATGA

Upstream 100 bases:

>100_bases
GAAAATTTACGCTATACTCATGAGGAAGACATGATGGAGTCAAGTGACCTTACTTTACGGCTCAATCTATTGTACGACTC
TGTTCGAAGGAGGAATTTTC

Downstream 100 bases:

>100_bases
CGTCATTTTGCAGACGGCCGCGATTATCATTTTCTTCATCATTGTCATCTTCTCCATCGACTTGTTCTTTGCCGGTCACT
ATTCACCGGGTGGAGGATTC

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 813; Mature: 812

Protein sequence:

>813_residues
MSLLHLAILLPLLAAPFIPLLFRGMKQIHTGWFVLIVPVVLVTYFAGFIPITSAGDVVTETIAWMPSLGINFTAYVDGLS
LLFALLITGIGALVVLYSIYYLNKDKESLGHFYVYLLMFMSAMLGVVLSDNMIVMYMFWELTSISSFLLIGYWYEKERSR
YGAQKSMLLTVFGGLSMLGGIVLLESMVGSYSIRDMVANSGVIAESPFFLVAMVLILLGAFTKSAQFPFHIWLPDAMEAP
TPVSAYLHSATMVKAGLYLVARMSPVFQESSVWLWTIAGVGMFTMFWGSFNAVKQTDLKGILAFSTVSQLGLIMSLLGLG
AAALHYDGMDDNIFMVATIAAIFHLINHATFKGSLFMMVGIVDHETGTRDIRKLGGLMTVMPISFTVAVIGSLSMAGLPP
FNGFLSKEMFLKATTKVFSADFFALDTIGILLPVIAFVGSVFTFIYSIMLFTRTFMGKAKFDQLPKQPHEAPIGMLISPV
ILALLVVGLGFFPNVLSNSLIKPAVESIYPTLVAQGQDVKVDIYFWHGLTPELYMTIGVILLGSLLFWQMGKWMGIYNRI
PHRLTLNGMYDGTLVWSNKAAYRFKDNYMTGFIRSYLIYIFVFLSGMMLFSIWWFDMYRFSFENLAEIAPYEYVLGAVVM
LAALAILFMKSRLPSIILLGVVGYGVALFFVFFGAPDLALTQLVIETVSVALFLLVFYHMPDISKKEVRMRFKVGNAIVA
VLVGTTVTLLSFMALTNKSLTSISEYYKENVYELAAGKNMVNVVLVDFRGFDTLFEIVVLGIAAFGIYTMIKFRTTRRTK
GEGEHEAKQQTRQ

Sequences:

>Translated_813_residues
MSLLHLAILLPLLAAPFIPLLFRGMKQIHTGWFVLIVPVVLVTYFAGFIPITSAGDVVTETIAWMPSLGINFTAYVDGLS
LLFALLITGIGALVVLYSIYYLNKDKESLGHFYVYLLMFMSAMLGVVLSDNMIVMYMFWELTSISSFLLIGYWYEKERSR
YGAQKSMLLTVFGGLSMLGGIVLLESMVGSYSIRDMVANSGVIAESPFFLVAMVLILLGAFTKSAQFPFHIWLPDAMEAP
TPVSAYLHSATMVKAGLYLVARMSPVFQESSVWLWTIAGVGMFTMFWGSFNAVKQTDLKGILAFSTVSQLGLIMSLLGLG
AAALHYDGMDDNIFMVATIAAIFHLINHATFKGSLFMMVGIVDHETGTRDIRKLGGLMTVMPISFTVAVIGSLSMAGLPP
FNGFLSKEMFLKATTKVFSADFFALDTIGILLPVIAFVGSVFTFIYSIMLFTRTFMGKAKFDQLPKQPHEAPIGMLISPV
ILALLVVGLGFFPNVLSNSLIKPAVESIYPTLVAQGQDVKVDIYFWHGLTPELYMTIGVILLGSLLFWQMGKWMGIYNRI
PHRLTLNGMYDGTLVWSNKAAYRFKDNYMTGFIRSYLIYIFVFLSGMMLFSIWWFDMYRFSFENLAEIAPYEYVLGAVVM
LAALAILFMKSRLPSIILLGVVGYGVALFFVFFGAPDLALTQLVIETVSVALFLLVFYHMPDISKKEVRMRFKVGNAIVA
VLVGTTVTLLSFMALTNKSLTSISEYYKENVYELAAGKNMVNVVLVDFRGFDTLFEIVVLGIAAFGIYTMIKFRTTRRTK
GEGEHEAKQQTRQ
>Mature_812_residues
SLLHLAILLPLLAAPFIPLLFRGMKQIHTGWFVLIVPVVLVTYFAGFIPITSAGDVVTETIAWMPSLGINFTAYVDGLSL
LFALLITGIGALVVLYSIYYLNKDKESLGHFYVYLLMFMSAMLGVVLSDNMIVMYMFWELTSISSFLLIGYWYEKERSRY
GAQKSMLLTVFGGLSMLGGIVLLESMVGSYSIRDMVANSGVIAESPFFLVAMVLILLGAFTKSAQFPFHIWLPDAMEAPT
PVSAYLHSATMVKAGLYLVARMSPVFQESSVWLWTIAGVGMFTMFWGSFNAVKQTDLKGILAFSTVSQLGLIMSLLGLGA
AALHYDGMDDNIFMVATIAAIFHLINHATFKGSLFMMVGIVDHETGTRDIRKLGGLMTVMPISFTVAVIGSLSMAGLPPF
NGFLSKEMFLKATTKVFSADFFALDTIGILLPVIAFVGSVFTFIYSIMLFTRTFMGKAKFDQLPKQPHEAPIGMLISPVI
LALLVVGLGFFPNVLSNSLIKPAVESIYPTLVAQGQDVKVDIYFWHGLTPELYMTIGVILLGSLLFWQMGKWMGIYNRIP
HRLTLNGMYDGTLVWSNKAAYRFKDNYMTGFIRSYLIYIFVFLSGMMLFSIWWFDMYRFSFENLAEIAPYEYVLGAVVML
AALAILFMKSRLPSIILLGVVGYGVALFFVFFGAPDLALTQLVIETVSVALFLLVFYHMPDISKKEVRMRFKVGNAIVAV
LVGTTVTLLSFMALTNKSLTSISEYYKENVYELAAGKNMVNVVLVDFRGFDTLFEIVVLGIAAFGIYTMIKFRTTRRTKG
EGEHEAKQQTRQ

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=388, Percent_Identity=32.4742268041237, Blast_Score=189, Evalue=8e-48,
Organism=Escherichia coli, GI1788614, Length=581, Percent_Identity=30.9810671256454, Blast_Score=205, Evalue=9e-54,
Organism=Escherichia coli, GI1788829, Length=396, Percent_Identity=33.0808080808081, Blast_Score=179, Evalue=7e-46,
Organism=Escherichia coli, GI1788831, Length=357, Percent_Identity=32.7731092436975, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788827, Length=460, Percent_Identity=26.5217391304348, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1788613, Length=416, Percent_Identity=28.8461538461538, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI2367154, Length=262, Percent_Identity=30.5343511450382, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI145693160, Length=358, Percent_Identity=28.2122905027933, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 90404; Mature: 90273

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SLLHLAILLPLLAAPFIPLLFRGMKQIHTGWFVLIVPVVLVTYFAGFIPITSAGDVVTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
TIAWMPSLGINFTAYVDGLSLLFALLITGIGALVVLYSIYYLNKDKESLGHFYVYLLMFM
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SAMLGVVLSDNMIVMYMFWELTSISSFLLIGYWYEKERSRYGAQKSMLLTVFGGLSMLGG
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IVLLESMVGSYSIRDMVANSGVIAESPFFLVAMVLILLGAFTKSAQFPFHIWLPDAMEAP
HHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
TPVSAYLHSATMVKAGLYLVARMSPVFQESSVWLWTIAGVGMFTMFWGSFNAVKQTDLKG
CCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHH
ILAFSTVSQLGLIMSLLGLGAAALHYDGMDDNIFMVATIAAIFHLINHATFKGSLFMMVG
HHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEE
IVDHETGTRDIRKLGGLMTVMPISFTVAVIGSLSMAGLPPFNGFLSKEMFLKATTKVFSA
EEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
DFFALDTIGILLPVIAFVGSVFTFIYSIMLFTRTFMGKAKFDQLPKQPHEAPIGMLISPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHH
ILALLVVGLGFFPNVLSNSLIKPAVESIYPTLVAQGQDVKVDIYFWHGLTPELYMTIGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHH
LLGSLLFWQMGKWMGIYNRIPHRLTLNGMYDGTLVWSNKAAYRFKDNYMTGFIRSYLIYI
HHHHHHHHHHHHHHHHHHCCCCEEEECCEECCEEEECCCCCEEECCHHHHHHHHHHHHHH
FVFLSGMMLFSIWWFDMYRFSFENLAEIAPYEYVLGAVVMLAALAILFMKSRLPSIILLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VVGYGVALFFVFFGAPDLALTQLVIETVSVALFLLVFYHMPDISKKEVRMRFKVGNAIVA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VLVGTTVTLLSFMALTNKSLTSISEYYKENVYELAAGKNMVNVVLVDFRGFDTLFEIVVL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHH
GIAAFGIYTMIKFRTTRRTKGEGEHEAKQQTRQ
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]