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The map label for this gene is cwlO [H]

Identifier: 229918283

GI number: 229918283

Start: 2525974

End: 2527269

Strand: Direct

Name: cwlO [H]

Synonym: EAT1b_2566

Alternate gene names: 229918283

Gene position: 2525974-2527269 (Clockwise)

Preceding gene: 229918282

Following gene: 229918287

Centisome position: 84.2

GC content: 48.3

Gene sequence:

>1296_bases
TTGGGTAACTTGAAGAAATTTGCAGCTACAGTCACTCTAGGCGCACTCTTGATCGGCGGAACGTCACCGACTTATGCAGC
CTCACTTACAGAGAAGCAGAAGAAAGTACAAGAACAACGACAAAAGAATAGCAGTGAACAGTCGAAAGCAAACTCTTCGA
TTCAATCACGTGAGCAAGCCATCAGCAAAGAGCAGCAAGAGATCAACAAGTTAGATGCTGAACTCCAAGACGTCATCAAC
GATGTCGCTCAGAAAAAAGCTGAGATTCGTGCAACTGAAGAAAAAATTAAAAAGCTTCAAGCACAAATTAAAAAGTACGA
AGCGAAAATGAAGGCACAAGAAGATCTTATGAAAGATCGTATGGCGACGATGCAAAAGAACGGCGGCTCGTCAGTCAACT
GGGCTGAGTTCATCTTCGGTTCTAAAGACTTCGGTGATTTGATCACACGCATGATCACAGCTGGTACGATTCAAGAGAAC
GACCAACAGATCTTCGAAGAGTATGAAGCAACGAAAGAAAAACTTGCGAAAGCGCAAGCTGATTTGAAAGCAGAGCGCGA
TTCACTCGTGAAGCAAAAAGAAGATCTTGAAAAACGTCAGAAAGAGCTCGATCAAAAAATGAAAGAGCGCGCTGCGAAAA
TCAAGAAACTTGAAGCTGAAAAAGTGAAGTTCGAATCAAAACTTATGAGCCTTCAAGAGATGGAAGATACGCTCAAAGCT
CAAGAACAAGCCATTCAAGCAGAAATCGAAGCGCAACGTCGTGCTGAAGAAGAAGCACGTCGTGCAGCGGCAGCAGCTGC
GGCAGCGAAAAAACAACAAGGTTCAAGCTCGAACAGCGGTTCAAGTGCTTCAACAGCACCGGTAGCGTCTGCACCAGCAA
GCGGTGGTATGTTCCAACGCCCAGCAAGCGGTCGCCTCTCACAAGGATGGGGTCCAGCAAGCGGTGCGAACGGCTACTCG
TTCCACAACGGTCTTGATATCGCAGGTCCAGTCGGTACACCGATCTATGCAGCACAAACTGGTACGGTCCTCCGTGCAGG
TTGGGGTGGCGCATACGGAAACCACGTCATGATTGCACACGTGATCAACGGGCAAGTATGGACGACAGTATACGCTCACA
TGAGCTCGGTATCCGTTTCTGCGGGTCAACGCGTGTCACAAGGACAAAACCTTGGCGGCATGGGTAGCACAGGGAACTCG
ACAGGTTCACACCTTCACTTCGAGATTCACCGTGGCGGCTACTCGTACTCTTCATCGAGCGCGGGAAGCACAGTCAACCC
AGCATCATTCTTCTAA

Upstream 100 bases:

>100_bases
ATGAGTCACAGAGCGAACGAAATGTCGCGACTTATCTTATTAAAAAATTACTGCACGACTATAGATAAAAACTACACGGG
AATCAGCGGAGGGAAGCACA

Downstream 100 bases:

>100_bases
ACACAAAGACAGTGATCGACGAACGCGTCGATCACTTTTTTGTTGCCTGATAGACGATGAACGTCAAAAGGGCGGCACCG
ATGACACTGAGCCATAGCGT

Product: peptidase M23

Products: NA

Alternate protein names: D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGNLKKFAATVTLGALLIGGTSPTYAASLTEKQKKVQEQRQKNSSEQSKANSSIQSREQAISKEQQEINKLDAELQDVIN
DVAQKKAEIRATEEKIKKLQAQIKKYEAKMKAQEDLMKDRMATMQKNGGSSVNWAEFIFGSKDFGDLITRMITAGTIQEN
DQQIFEEYEATKEKLAKAQADLKAERDSLVKQKEDLEKRQKELDQKMKERAAKIKKLEAEKVKFESKLMSLQEMEDTLKA
QEQAIQAEIEAQRRAEEEARRAAAAAAAAKKQQGSSSNSGSSASTAPVASAPASGGMFQRPASGRLSQGWGPASGANGYS
FHNGLDIAGPVGTPIYAAQTGTVLRAGWGGAYGNHVMIAHVINGQVWTTVYAHMSSVSVSAGQRVSQGQNLGGMGSTGNS
TGSHLHFEIHRGGYSYSSSSAGSTVNPASFF

Sequences:

>Translated_431_residues
MGNLKKFAATVTLGALLIGGTSPTYAASLTEKQKKVQEQRQKNSSEQSKANSSIQSREQAISKEQQEINKLDAELQDVIN
DVAQKKAEIRATEEKIKKLQAQIKKYEAKMKAQEDLMKDRMATMQKNGGSSVNWAEFIFGSKDFGDLITRMITAGTIQEN
DQQIFEEYEATKEKLAKAQADLKAERDSLVKQKEDLEKRQKELDQKMKERAAKIKKLEAEKVKFESKLMSLQEMEDTLKA
QEQAIQAEIEAQRRAEEEARRAAAAAAAAKKQQGSSSNSGSSASTAPVASAPASGGMFQRPASGRLSQGWGPASGANGYS
FHNGLDIAGPVGTPIYAAQTGTVLRAGWGGAYGNHVMIAHVINGQVWTTVYAHMSSVSVSAGQRVSQGQNLGGMGSTGNS
TGSHLHFEIHRGGYSYSSSSAGSTVNPASFF
>Mature_430_residues
GNLKKFAATVTLGALLIGGTSPTYAASLTEKQKKVQEQRQKNSSEQSKANSSIQSREQAISKEQQEINKLDAELQDVIND
VAQKKAEIRATEEKIKKLQAQIKKYEAKMKAQEDLMKDRMATMQKNGGSSVNWAEFIFGSKDFGDLITRMITAGTIQEND
QQIFEEYEATKEKLAKAQADLKAERDSLVKQKEDLEKRQKELDQKMKERAAKIKKLEAEKVKFESKLMSLQEMEDTLKAQ
EQAIQAEIEAQRRAEEEARRAAAAAAAAKKQQGSSSNSGSSASTAPVASAPASGGMFQRPASGRLSQGWGPASGANGYSF
HNGLDIAGPVGTPIYAAQTGTVLRAGWGGAYGNHVMIAHVINGQVWTTVYAHMSSVSVSAGQRVSQGQNLGGMGSTGNST
GSHLHFEIHRGGYSYSSSSAGSTVNPASFF

Specific function: Shows a cell wall hydrolytic DL-endopeptidase activity [H]

COG id: COG0739

COG function: function code M; Membrane proteins related to metalloendopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C40 family [H]

Homologues:

Organism=Escherichia coli, GI87081989, Length=88, Percent_Identity=42.0454545454545, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082174, Length=131, Percent_Identity=36.6412213740458, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 46551; Mature: 46419

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCC
>Mature Secondary Structure 
GNLKKFAATVTLGALLIGGTSPTYAASLTEKQKKVQEQRQKNSSEQSKANSSIQSREQA
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ISKEQQEINKLDAELQDVINDVAQKKAEIRATEEKIKKLQAQIKKYEAKMKAQEDLMKDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MATMQKNGGSSVNWAEFIFGSKDFGDLITRMITAGTIQENDQQIFEEYEATKEKLAKAQA
HHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DLKAERDSLVKQKEDLEKRQKELDQKMKERAAKIKKLEAEKVKFESKLMSLQEMEDTLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEQAIQAEIEAQRRAEEEARRAAAAAAAAKKQQGSSSNSGSSASTAPVASAPASGGMFQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PASGRLSQGWGPASGANGYSFHNGLDIAGPVGTPIYAAQTGTVLRAGWGGAYGNHVMIAH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCEEEEE
VINGQVWTTVYAHMSSVSVSAGQRVSQGQNLGGMGSTGNSTGSHLHFEIHRGGYSYSSSS
EECCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
AGSTVNPASFF
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA