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The map label for this gene is odhB [H]

Identifier: 229918257

GI number: 229918257

Start: 2500988

End: 2502262

Strand: Direct

Name: odhB [H]

Synonym: EAT1b_2539

Alternate gene names: 229918257

Gene position: 2500988-2502262 (Clockwise)

Preceding gene: 229918256

Following gene: 229918259

Centisome position: 83.37

GC content: 51.69

Gene sequence:

>1275_bases
GTGATCGAAATCAAAGTACCGGAGTTAGCAGAGTCCATTACGGAAGGAACGGTCGCGACATGGCTCAAACAGCCAGGCGA
CCAAGTCGAAAAAGGGGAAGCCATCGTCGAGCTCGAGACAGATAAAGTCAATATCGAAGTCCCGGCTGACGAAGCGGGTG
TGTTAGAAGAACAACTTGCCGGAGAAGGGGACACGGTTCAAGTCGGTGAAGTCATCGCACGTCTCGGTTCAGGTTCAGGT
GGTGGTACGGCTGTCGCGACGAAGACGAAGACGGAAAACGCGACTGAAACGAAGACAGAAGCACCGACTGAGAAAAAGAC
GGAGTCTGTCGAGGAAGGCAAGAAAGTTGAGAAACGTGAAGAACACGTTGCGAGCCCTGGTAAAGGACCAATCGCGACAC
CGGCTGCACGTAAACTCGCTCGTGAGAAAGGCATTGATCTCTCAGCCGTTCAAACGAATGATCCAATCGGGCGCATCAAC
GTCCATGATGTATCGAGACATGAAGAGAAACCGGCTGTGAAAGAGCAGAAGCCAGCGGCAGCAGCTCCGGCACCACAACC
GGCTGCAAGTGGAAAAGAAGAAGAGCGCATCAAAATGACACGTCGCCGTCAGACGATCGCGAACCGTCTCGTCGAAGTGC
AACAGACGGCAGCGATGCTCACGACGTTCAATGAAATCGACATGTCTGCGGTCATGGCACTTCGTAAACGTCGCCAAGAG
AAGTTCGTGAAAGACAACGATGTCAAACTCGGCTTCATGTCATTCTTCACGAAAGCAGCGGTAGCGGCTCTCAAGCGGAT
GCCTTACTTGAACGCTGAAATCCAAGGCAACGAAATCGTCTTGAAGAAATATTATGACATCGGAATCGCCGTTTCGGCTC
CAGATGGTCTCGTCGTTCCGGTCGTCCGTGAAGCGGATCGCAAAAACTTCGGGGAGATCGAGAAAGACATCCTTCACCTT
GCGGACAAAGCACGTAACAACAAGCTCGGGTTGAGTGATTTGACAGGTGGTACGTTCACGATCACAAACGGTGGCGTGTT
CGGTTCACTCCTCTCAACACCAATCTTGAACGGACCGCAAGTCGCTATCCTTGGGATGCACTCGATTCAACTCCGTCCAG
TCGCAATCGATGCGGATACAATGGAGAACCGTCCGATGATGTACGTGGCACTCTCGTATGACCACCGAATCGTAGATGGT
CGTGAGGCTGTGACATTCCTCAAGCACATTAAAGACATGATTGAAGATCCAGAACAATTGTTGTTCGAAGCGTAA

Upstream 100 bases:

>100_bases
ACGCATCATCCGTGAAGCACTCTCTCGTGACGTCGTCTCGAGCGGTGCAGGCACGAGCACATATCAAAAAGATCGTAAAT
AAGTTAAGGGGGAACTCATT

Downstream 100 bases:

>100_bases
GAACAGGAAGTACTCGCTCCTATTTAGGGGCGAGTGCTTTTTTATGTCAGGTTTTAGAAAAAATCTTATTATAGGTTTTT
TTGTTGAGTAAATATTTATG

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MIEIKVPELAESITEGTVATWLKQPGDQVEKGEAIVELETDKVNIEVPADEAGVLEEQLAGEGDTVQVGEVIARLGSGSG
GGTAVATKTKTENATETKTEAPTEKKTESVEEGKKVEKREEHVASPGKGPIATPAARKLAREKGIDLSAVQTNDPIGRIN
VHDVSRHEEKPAVKEQKPAAAAPAPQPAASGKEEERIKMTRRRQTIANRLVEVQQTAAMLTTFNEIDMSAVMALRKRRQE
KFVKDNDVKLGFMSFFTKAAVAALKRMPYLNAEIQGNEIVLKKYYDIGIAVSAPDGLVVPVVREADRKNFGEIEKDILHL
ADKARNNKLGLSDLTGGTFTITNGGVFGSLLSTPILNGPQVAILGMHSIQLRPVAIDADTMENRPMMYVALSYDHRIVDG
REAVTFLKHIKDMIEDPEQLLFEA

Sequences:

>Translated_424_residues
MIEIKVPELAESITEGTVATWLKQPGDQVEKGEAIVELETDKVNIEVPADEAGVLEEQLAGEGDTVQVGEVIARLGSGSG
GGTAVATKTKTENATETKTEAPTEKKTESVEEGKKVEKREEHVASPGKGPIATPAARKLAREKGIDLSAVQTNDPIGRIN
VHDVSRHEEKPAVKEQKPAAAAPAPQPAASGKEEERIKMTRRRQTIANRLVEVQQTAAMLTTFNEIDMSAVMALRKRRQE
KFVKDNDVKLGFMSFFTKAAVAALKRMPYLNAEIQGNEIVLKKYYDIGIAVSAPDGLVVPVVREADRKNFGEIEKDILHL
ADKARNNKLGLSDLTGGTFTITNGGVFGSLLSTPILNGPQVAILGMHSIQLRPVAIDADTMENRPMMYVALSYDHRIVDG
REAVTFLKHIKDMIEDPEQLLFEA
>Mature_424_residues
MIEIKVPELAESITEGTVATWLKQPGDQVEKGEAIVELETDKVNIEVPADEAGVLEEQLAGEGDTVQVGEVIARLGSGSG
GGTAVATKTKTENATETKTEAPTEKKTESVEEGKKVEKREEHVASPGKGPIATPAARKLAREKGIDLSAVQTNDPIGRIN
VHDVSRHEEKPAVKEQKPAAAAPAPQPAASGKEEERIKMTRRRQTIANRLVEVQQTAAMLTTFNEIDMSAVMALRKRRQE
KFVKDNDVKLGFMSFFTKAAVAALKRMPYLNAEIQGNEIVLKKYYDIGIAVSAPDGLVVPVVREADRKNFGEIEKDILHL
ADKARNNKLGLSDLTGGTFTITNGGVFGSLLSTPILNGPQVAILGMHSIQLRPVAIDADTMENRPMMYVALSYDHRIVDG
REAVTFLKHIKDMIEDPEQLLFEA

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=255, Percent_Identity=55.2941176470588, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=29.3302540415704, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI110671329, Length=452, Percent_Identity=27.4336283185841, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=28.0434782608696, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=28.695652173913, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=33.1325301204819, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1786946, Length=422, Percent_Identity=47.39336492891, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1786305, Length=434, Percent_Identity=30.6451612903226, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=424, Percent_Identity=41.5094339622642, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=30.6818181818182, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=27.9445727482679, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=235, Percent_Identity=31.4893617021277, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=41.0926365795724, Blast_Score=305, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=30.3296703296703, Blast_Score=169, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=53.9823008849557, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=26.8348623853211, Blast_Score=148, Evalue=5e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46146; Mature: 46146

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIKVPELAESITEGTVATWLKQPGDQVEKGEAIVELETDKVNIEVPADEAGVLEEQLA
CEEEECHHHHHHHHCCHHHHHHHCCCCHHHCCCEEEEEECCEEEEEECCCHHHHHHHHHC
GEGDTVQVGEVIARLGSGSGGGTAVATKTKTENATETKTEAPTEKKTESVEEGKKVEKRE
CCCCEEHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EHVASPGKGPIATPAARKLAREKGIDLSAVQTNDPIGRINVHDVSRHEEKPAVKEQKPAA
HHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEECCCHHCCCCCCCHHCCCCC
AAPAPQPAASGKEEERIKMTRRRQTIANRLVEVQQTAAMLTTFNEIDMSAVMALRKRRQE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFVKDNDVKLGFMSFFTKAAVAALKRMPYLNAEIQGNEIVLKKYYDIGIAVSAPDGLVVP
HCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEEEEEEEEEECCCCEEEH
VVREADRKNFGEIEKDILHLADKARNNKLGLSDLTGGTFTITNGGVFGSLLSTPILNGPQ
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCC
VAILGMHSIQLRPVAIDADTMENRPMMYVALSYDHRIVDGREAVTFLKHIKDMIEDPEQL
EEEEEEEEEEEEEEEEECCCCCCCCEEEEEEECCCEECCHHHHHHHHHHHHHHHCCHHHH
LFEA
HCCC
>Mature Secondary Structure
MIEIKVPELAESITEGTVATWLKQPGDQVEKGEAIVELETDKVNIEVPADEAGVLEEQLA
CEEEECHHHHHHHHCCHHHHHHHCCCCHHHCCCEEEEEECCEEEEEECCCHHHHHHHHHC
GEGDTVQVGEVIARLGSGSGGGTAVATKTKTENATETKTEAPTEKKTESVEEGKKVEKRE
CCCCEEHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EHVASPGKGPIATPAARKLAREKGIDLSAVQTNDPIGRINVHDVSRHEEKPAVKEQKPAA
HHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEECCCHHCCCCCCCHHCCCCC
AAPAPQPAASGKEEERIKMTRRRQTIANRLVEVQQTAAMLTTFNEIDMSAVMALRKRRQE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFVKDNDVKLGFMSFFTKAAVAALKRMPYLNAEIQGNEIVLKKYYDIGIAVSAPDGLVVP
HCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEEEEEEEEEECCCCEEEH
VVREADRKNFGEIEKDILHLADKARNNKLGLSDLTGGTFTITNGGVFGSLLSTPILNGPQ
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCC
VAILGMHSIQLRPVAIDADTMENRPMMYVALSYDHRIVDGREAVTFLKHIKDMIEDPEQL
EEEEEEEEEEEEEEEEECCCCCCCCEEEEEEECCCEECCHHHHHHHHHHHHHHHCCHHHH
LFEA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]