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The map label for this gene is ytwF [H]

Identifier: 229918249

GI number: 229918249

Start: 2492267

End: 2492563

Strand: Reverse

Name: ytwF [H]

Synonym: EAT1b_2530

Alternate gene names: 229918249

Gene position: 2492563-2492267 (Counterclockwise)

Preceding gene: 229918252

Following gene: 229918248

Centisome position: 83.09

GC content: 49.49

Gene sequence:

>297_bases
ATGAAGACGATTACGACTACTGAATTAGAAGCGTTACTTCAAGCAGACGACTCACTCAACCTCATCGATGTGCGAGAGGT
AGACGAATATGCTGGAGGTCACATCAAACAAGCGAAGAACGTACCACTTTCTGAATTCGGGGCAAAAGTCGATGAGCTCG
ATCGCTCTAAACCGATTCACGTCATTTGCGCAGCCGGCGGACGCAGCATGAACGCATCTGCCTATCTCGATTCACTCGGA
TTCGATGTCACGAACGTCGACGGTGGAATGATGAGCTGGCAAGGCGAAGTCGAATAA

Upstream 100 bases:

>100_bases
TCTTTTCACTTGCGCTCATTATACCTTAGGGGGTATATTAAAGCCAACGAAACATTCTCGTTGCCAATAACCTTAACCAT
GAAAAGGAGATCACAGACTC

Downstream 100 bases:

>100_bases
ACGATTACATCCATAGGGGGAAATTGATATGCTATTACGTTATTTTTATGATGAAAAATTAGCACAAGCTTCATATATAG
TCGGTTGCCAAATGACGGGA

Product: Rhodanese domain protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MKTITTTELEALLQADDSLNLIDVREVDEYAGGHIKQAKNVPLSEFGAKVDELDRSKPIHVICAAGGRSMNASAYLDSLG
FDVTNVDGGMMSWQGEVE

Sequences:

>Translated_98_residues
MKTITTTELEALLQADDSLNLIDVREVDEYAGGHIKQAKNVPLSEFGAKVDELDRSKPIHVICAAGGRSMNASAYLDSLG
FDVTNVDGGMMSWQGEVE
>Mature_98_residues
MKTITTTELEALLQADDSLNLIDVREVDEYAGGHIKQAKNVPLSEFGAKVDELDRSKPIHVICAAGGRSMNASAYLDSLG
FDVTNVDGGMMSWQGEVE

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 10586; Mature: 10586

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTITTTELEALLQADDSLNLIDVREVDEYAGGHIKQAKNVPLSEFGAKVDELDRSKPIH
CCCCCHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCCCCCCCHHHHCCCHHHCCCCCCEE
VICAAGGRSMNASAYLDSLGFDVTNVDGGMMSWQGEVE
EEECCCCCCCCHHHHHHHCCCEEECCCCCEECCCCCCC
>Mature Secondary Structure
MKTITTTELEALLQADDSLNLIDVREVDEYAGGHIKQAKNVPLSEFGAKVDELDRSKPIH
CCCCCHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCCCCCCCHHHHCCCHHHCCCCCCEE
VICAAGGRSMNASAYLDSLGFDVTNVDGGMMSWQGEVE
EEECCCCCCCCHHHHHHHCCCEEECCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]