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The map label for this gene is glcA [C]

Identifier: 229918118

GI number: 229918118

Start: 2348372

End: 2350012

Strand: Direct

Name: glcA [C]

Synonym: EAT1b_2398

Alternate gene names: 229918118

Gene position: 2348372-2350012 (Clockwise)

Preceding gene: 229918117

Following gene: 229918119

Centisome position: 78.28

GC content: 51.37

Gene sequence:

>1641_bases
TTGACGATTTTAGAATGGATTACAGCACTGACACCGATTGTGGCCGTGCTCATCTTATTGGTCATTATGCGTTTACCTGC
ATCGGTAGCCATGCCCTTGTCACTCGTGACGACGGCAATTTTAGCGTTCTTCGTCTGGCAATTGGATGCGACACGAATTA
TGGCGGCGACATTACAAGGACTCGTCATCGCCGTCACGACAGTATGGATTGTCTACGGGGCAATCGGAATGATGAATACG
ATGAAAGAAGGGGGCGGGATGAACGCGATTCGCTCCGGATTCGTCAACATCACACCTGACCCGCGTATTCAAGTTTTGAT
TGTCGGATGGTTATTCGTTTCATTTATTGAAGGCGCATCTGGATTCGGGACGCCTGCAACGGTTGCGGCACCGCTCTTGA
TCGCACTCGGATTTCCGCCGATTGCCGCCGTTGTCATTGCATTATCCGCAGACGTGTCGGCTGTGACATTTGGTGCGGTC
GGGACACCGGCACTTATCGGGATCGGGAACGGTCTATCTTTAGATGTGAACCAGCCTGAGTCGATTGCCTTCTTGACAGA
GGTCGTCTTACAGACGGTGTTGATCGACATGGTAGTCGGTCTATTCATCCCGTTCATTTTAATTTTGATGTTGACGCGTT
TCTTCAGTAAGGAAAAGTCGATAAAACCTGCACTCGAGGTATTTCCGATCGCGATTGTGGCGGCGCTGTTGTACAGCGTA
CCGGCGTACATTTGGACACTCATCCTCGGGTTTGAGTTCGGTGGCATGCTCGGTGGTTTAACCGGTCTCGTCATCCTCGT
GGCCATGGCTCGTAAAGGCATTCTCATGCCGAAACGCGTCTGGACGTTTGATGGATATGTGGATGAACTAGAACCATCTA
CTGAGAAAGTGACGCTCAAATCAACCACGCGTGAATTACCACAGTGGAAGGCGTGGGCACCTTATCTCATTTTGACGATT
TTCTTAGTGCTCACCCGTACTGTCCCATTTTTAAACGAACTCTCTCGCAGTGTCCGGATCAATCTCCCGGACATCCTCGG
GGTAGAAGGGGTTTCGACGTCTTGGGAACCGTTTTATTCACCAGGTTTTATGTTTATCCTGACGATTATTGCGACATTCT
TCTTACACAACATGAGTTGGTCACAAGTGACATCGGTGTTTAAGGCGACGACACTTGGAGTCGTCGGAACGGCCATCACG
TTAGCGGCGTCGGTTCCGATGGTTCGTATATTCATCCACTCTGAAAATGGCGGTGCCGGACTCGCTTCGATGCCGGTCGA
GCTTGCGACGGCAGCTGCTGACACGTTCGGTGGGGTGTGGCCACTTGTCGCACCGTGGATTGGGGCGCTCGGCGCCTTCG
TTTCAGGTTCGGCGACGTTTTCTAACATGATGTTCTCGAGTTTGACGACAAACGTTGCGGACTCGGTCGGACTCGATAAC
ACGCTCGCTTTATCGATGCAGATGGCCGGGGCGAACGCAGGAAACATGATCTGTGTGCTGAACGTCGTAGCGGTCGCTTC
CGTTGCGGGACTGCTCGGTAAAGAAGGAACCATCATCCGTTATACGCTCATGCCGATGGTTTACTACGTGACGGCCACAG
GGGTCATTGGTCTCATTTTAGGCATGTTTTTCTTAAACTGA

Upstream 100 bases:

>100_bases
GTATGATGTGATTGAACGTACGTTACATCGACTCGCTGAAGAAGAATCGTGATGGGACATCTGTTTACGGTAGGGGAAAA
CACGAAAAGGTGGGTCTACA

Downstream 100 bases:

>100_bases
ATCAAGTTACAATTTTTTTACAGAATAATGTTGACAATTTTCAGAACAGTCTGCTAAGGTTTGAGTAGATTTTAAAACTA
TGAAACGAGAGGAGCCAGAA

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTILEWITALTPIVAVLILLVIMRLPASVAMPLSLVTTAILAFFVWQLDATRIMAATLQGLVIAVTTVWIVYGAIGMMNT
MKEGGGMNAIRSGFVNITPDPRIQVLIVGWLFVSFIEGASGFGTPATVAAPLLIALGFPPIAAVVIALSADVSAVTFGAV
GTPALIGIGNGLSLDVNQPESIAFLTEVVLQTVLIDMVVGLFIPFILILMLTRFFSKEKSIKPALEVFPIAIVAALLYSV
PAYIWTLILGFEFGGMLGGLTGLVILVAMARKGILMPKRVWTFDGYVDELEPSTEKVTLKSTTRELPQWKAWAPYLILTI
FLVLTRTVPFLNELSRSVRINLPDILGVEGVSTSWEPFYSPGFMFILTIIATFFLHNMSWSQVTSVFKATTLGVVGTAIT
LAASVPMVRIFIHSENGGAGLASMPVELATAAADTFGGVWPLVAPWIGALGAFVSGSATFSNMMFSSLTTNVADSVGLDN
TLALSMQMAGANAGNMICVLNVVAVASVAGLLGKEGTIIRYTLMPMVYYVTATGVIGLILGMFFLN

Sequences:

>Translated_546_residues
MTILEWITALTPIVAVLILLVIMRLPASVAMPLSLVTTAILAFFVWQLDATRIMAATLQGLVIAVTTVWIVYGAIGMMNT
MKEGGGMNAIRSGFVNITPDPRIQVLIVGWLFVSFIEGASGFGTPATVAAPLLIALGFPPIAAVVIALSADVSAVTFGAV
GTPALIGIGNGLSLDVNQPESIAFLTEVVLQTVLIDMVVGLFIPFILILMLTRFFSKEKSIKPALEVFPIAIVAALLYSV
PAYIWTLILGFEFGGMLGGLTGLVILVAMARKGILMPKRVWTFDGYVDELEPSTEKVTLKSTTRELPQWKAWAPYLILTI
FLVLTRTVPFLNELSRSVRINLPDILGVEGVSTSWEPFYSPGFMFILTIIATFFLHNMSWSQVTSVFKATTLGVVGTAIT
LAASVPMVRIFIHSENGGAGLASMPVELATAAADTFGGVWPLVAPWIGALGAFVSGSATFSNMMFSSLTTNVADSVGLDN
TLALSMQMAGANAGNMICVLNVVAVASVAGLLGKEGTIIRYTLMPMVYYVTATGVIGLILGMFFLN
>Mature_545_residues
TILEWITALTPIVAVLILLVIMRLPASVAMPLSLVTTAILAFFVWQLDATRIMAATLQGLVIAVTTVWIVYGAIGMMNTM
KEGGGMNAIRSGFVNITPDPRIQVLIVGWLFVSFIEGASGFGTPATVAAPLLIALGFPPIAAVVIALSADVSAVTFGAVG
TPALIGIGNGLSLDVNQPESIAFLTEVVLQTVLIDMVVGLFIPFILILMLTRFFSKEKSIKPALEVFPIAIVAALLYSVP
AYIWTLILGFEFGGMLGGLTGLVILVAMARKGILMPKRVWTFDGYVDELEPSTEKVTLKSTTRELPQWKAWAPYLILTIF
LVLTRTVPFLNELSRSVRINLPDILGVEGVSTSWEPFYSPGFMFILTIIATFFLHNMSWSQVTSVFKATTLGVVGTAITL
AASVPMVRIFIHSENGGAGLASMPVELATAAADTFGGVWPLVAPWIGALGAFVSGSATFSNMMFSSLTTNVADSVGLDNT
LALSMQMAGANAGNMICVLNVVAVASVAGLLGKEGTIIRYTLMPMVYYVTATGVIGLILGMFFLN

Specific function: May play a role in L-lactate transport [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=573, Percent_Identity=25.4799301919721, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790031, Length=553, Percent_Identity=26.9439421338156, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58050; Mature: 57919

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILEWITALTPIVAVLILLVIMRLPASVAMPLSLVTTAILAFFVWQLDATRIMAATLQG
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVIAVTTVWIVYGAIGMMNTMKEGGGMNAIRSGFVNITPDPRIQVLIVGWLFVSFIEGAS
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEECCCCCCEEEEHHHHHHHHHHHCCC
GFGTPATVAAPLLIALGFPPIAAVVIALSADVSAVTFGAVGTPALIGIGNGLSLDVNQPE
CCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCCEEECCCCCEECCCCCH
SIAFLTEVVLQTVLIDMVVGLFIPFILILMLTRFFSKEKSIKPALEVFPIAIVAALLYSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
PAYIWTLILGFEFGGMLGGLTGLVILVAMARKGILMPKRVWTFDGYVDELEPSTEKVTLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCCCEEEEH
STTRELPQWKAWAPYLILTIFLVLTRTVPFLNELSRSVRINLPDILGVEGVSTSWEPFYS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHCCCCCCCCCCCCCCC
PGFMFILTIIATFFLHNMSWSQVTSVFKATTLGVVGTAITLAASVPMVRIFIHSENGGAG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
LASMPVELATAAADTFGGVWPLVAPWIGALGAFVSGSATFSNMMFSSLTTNVADSVGLDN
CHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
TLALSMQMAGANAGNMICVLNVVAVASVAGLLGKEGTIIRYTLMPMVYYVTATGVIGLIL
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
GMFFLN
HHHHCC
>Mature Secondary Structure 
TILEWITALTPIVAVLILLVIMRLPASVAMPLSLVTTAILAFFVWQLDATRIMAATLQG
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVIAVTTVWIVYGAIGMMNTMKEGGGMNAIRSGFVNITPDPRIQVLIVGWLFVSFIEGAS
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEECCCCCCEEEEHHHHHHHHHHHCCC
GFGTPATVAAPLLIALGFPPIAAVVIALSADVSAVTFGAVGTPALIGIGNGLSLDVNQPE
CCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCCEEECCCCCEECCCCCH
SIAFLTEVVLQTVLIDMVVGLFIPFILILMLTRFFSKEKSIKPALEVFPIAIVAALLYSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
PAYIWTLILGFEFGGMLGGLTGLVILVAMARKGILMPKRVWTFDGYVDELEPSTEKVTLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCCCEEEEH
STTRELPQWKAWAPYLILTIFLVLTRTVPFLNELSRSVRINLPDILGVEGVSTSWEPFYS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHCCCCCCCCCCCCCCC
PGFMFILTIIATFFLHNMSWSQVTSVFKATTLGVVGTAITLAASVPMVRIFIHSENGGAG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
LASMPVELATAAADTFGGVWPLVAPWIGALGAFVSGSATFSNMMFSSLTTNVADSVGLDN
CHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
TLALSMQMAGANAGNMICVLNVVAVASVAGLLGKEGTIIRYTLMPMVYYVTATGVIGLIL
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
GMFFLN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]