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The map label for this gene is liaS [H]

Identifier: 229918077

GI number: 229918077

Start: 2309982

End: 2311004

Strand: Direct

Name: liaS [H]

Synonym: EAT1b_2356

Alternate gene names: 229918077

Gene position: 2309982-2311004 (Clockwise)

Preceding gene: 229918076

Following gene: 229918078

Centisome position: 77.0

GC content: 49.36

Gene sequence:

>1023_bases
ATGAAAAAAGATTCTTATCCACGAACGGTCGTCTGGCATCAACTGTTTAGTAGCCTGTTGACGGCTTTCTTGATTACAGT
AGCGTTTCTCATAGGTGACTCGACTTCATTGAGCGATTTATTTTCGCGTGAAGAAGGGTTGCCTTTCGGTGTGTCCATCC
TGGTGATTGCCGTCTTGATCGGAAGTATCTTTGGCGTCGGTTCGTATTTTTATTTGCGCCGAGACTTTCGAGAAGTCGCC
AATGCCCTTTGGAAACTGGAGAACCAAAAAGACATCACTTTCCGCCCGATGTCCCCATACCGAGATACACTCGAACGAAT
CGTCCGCGTATCGAAACAGCGCCAAGAGATGGTCGAGATGGCGAAGCGAGTCGGTGAGCGTCCGGTCAGTGTGGAAGAGG
CGCGGAGTGAGGCCGTCGTCGAAGAAAGACGTCGCGTCGCACGAGAACTTCATGATTCGGTGAGTCAACAGCTCTATGCC
ATCTCGATGATGACGACAGCGGTCAAACAGACGATGCAGACGAAACCAGATCTCGCGAGTAAACAAATGGAACAAGTCGA
GCTCATGGCGCAGACCGCACAGGCAGAGATGCGTTCGTTGCTGTTACAACTTCGGCCGGTCGAACTAGAAGGCATGACAC
TAAAGACGGGAATTGAACGCTTGTTAGAAGAACTGTCTCGTAAACAATCGACCGAGCTCGTCTGGCGTTTAGAGGAAGTG
AAGCTCTCCCGGCACACTGAAAATGAACTGTTTCGGATTGTTCAGGAAGCGATTAGTAACACGTTACGACACGCCAAAGC
GACTCGTTTAGAAATCGAGATGCGAACGGTGCAACAGACGGTCATCTTAAAGATTAACGATGACGGAATCGGATTTAATG
TGGACGATACACAAGTCGCCTCATATGGATTACAATCGATAAGAGAGCGTACGGCAGAAGTCGGTGGAACGTTGCGATTG
GTCAGTGTGCCGAATGTCGGGACACAAATTGAAGTACGTGTGAAACAACAAGTGGAGGGGTAA

Upstream 100 bases:

>100_bases
ATCGTCGCTTCTATACAGCGTCTGATGATTATGTCCAGGCCACGCGAAAAGTCCGGATTCATATCGTGCTCATGAGCGGA
AGTGTCGAGGTGATGACGGT

Downstream 100 bases:

>100_bases
TGATGATTCGAGTTGTATTAATTGATGATCATGAAATGGTTCGGGCGGGCGTATCTGCCTTTTTATCGACACAACCGGAC
ATCGAAGTCGTCGGCGAAGC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKKDSYPRTVVWHQLFSSLLTAFLITVAFLIGDSTSLSDLFSREEGLPFGVSILVIAVLIGSIFGVGSYFYLRRDFREVA
NALWKLENQKDITFRPMSPYRDTLERIVRVSKQRQEMVEMAKRVGERPVSVEEARSEAVVEERRRVARELHDSVSQQLYA
ISMMTTAVKQTMQTKPDLASKQMEQVELMAQTAQAEMRSLLLQLRPVELEGMTLKTGIERLLEELSRKQSTELVWRLEEV
KLSRHTENELFRIVQEAISNTLRHAKATRLEIEMRTVQQTVILKINDDGIGFNVDDTQVASYGLQSIRERTAEVGGTLRL
VSVPNVGTQIEVRVKQQVEG

Sequences:

>Translated_340_residues
MKKDSYPRTVVWHQLFSSLLTAFLITVAFLIGDSTSLSDLFSREEGLPFGVSILVIAVLIGSIFGVGSYFYLRRDFREVA
NALWKLENQKDITFRPMSPYRDTLERIVRVSKQRQEMVEMAKRVGERPVSVEEARSEAVVEERRRVARELHDSVSQQLYA
ISMMTTAVKQTMQTKPDLASKQMEQVELMAQTAQAEMRSLLLQLRPVELEGMTLKTGIERLLEELSRKQSTELVWRLEEV
KLSRHTENELFRIVQEAISNTLRHAKATRLEIEMRTVQQTVILKINDDGIGFNVDDTQVASYGLQSIRERTAEVGGTLRL
VSVPNVGTQIEVRVKQQVEG
>Mature_340_residues
MKKDSYPRTVVWHQLFSSLLTAFLITVAFLIGDSTSLSDLFSREEGLPFGVSILVIAVLIGSIFGVGSYFYLRRDFREVA
NALWKLENQKDITFRPMSPYRDTLERIVRVSKQRQEMVEMAKRVGERPVSVEEARSEAVVEERRRVARELHDSVSQQLYA
ISMMTTAVKQTMQTKPDLASKQMEQVELMAQTAQAEMRSLLLQLRPVELEGMTLKTGIERLLEELSRKQSTELVWRLEEV
KLSRHTENELFRIVQEAISNTLRHAKATRLEIEMRTVQQTVILKINDDGIGFNVDDTQVASYGLQSIRERTAEVGGTLRL
VSVPNVGTQIEVRVKQQVEG

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=239, Percent_Identity=29.7071129707113, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788812, Length=202, Percent_Identity=28.2178217821782, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787474, Length=196, Percent_Identity=27.5510204081633, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38845; Mature: 38845

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDSYPRTVVWHQLFSSLLTAFLITVAFLIGDSTSLSDLFSREEGLPFGVSILVIAVLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
GSIFGVGSYFYLRRDFREVANALWKLENQKDITFRPMSPYRDTLERIVRVSKQRQEMVEM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
AKRVGERPVSVEEARSEAVVEERRRVARELHDSVSQQLYAISMMTTAVKQTMQTKPDLAS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KQMEQVELMAQTAQAEMRSLLLQLRPVELEGMTLKTGIERLLEELSRKQSTELVWRLEEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLSRHTENELFRIVQEAISNTLRHAKATRLEIEMRTVQQTVILKINDDGIGFNVDDTQVA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHH
SYGLQSIRERTAEVGGTLRLVSVPNVGTQIEVRVKQQVEG
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHCCCC
>Mature Secondary Structure
MKKDSYPRTVVWHQLFSSLLTAFLITVAFLIGDSTSLSDLFSREEGLPFGVSILVIAVLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
GSIFGVGSYFYLRRDFREVANALWKLENQKDITFRPMSPYRDTLERIVRVSKQRQEMVEM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
AKRVGERPVSVEEARSEAVVEERRRVARELHDSVSQQLYAISMMTTAVKQTMQTKPDLAS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KQMEQVELMAQTAQAEMRSLLLQLRPVELEGMTLKTGIERLLEELSRKQSTELVWRLEEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLSRHTENELFRIVQEAISNTLRHAKATRLEIEMRTVQQTVILKINDDGIGFNVDDTQVA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHH
SYGLQSIRERTAEVGGTLRLVSVPNVGTQIEVRVKQQVEG
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]