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The map label for this gene is dapA

Identifier: 229917983

GI number: 229917983

Start: 2216461

End: 2217342

Strand: Direct

Name: dapA

Synonym: EAT1b_2262

Alternate gene names: 229917983

Gene position: 2216461-2217342 (Clockwise)

Preceding gene: 229917982

Following gene: 229917984

Centisome position: 73.88

GC content: 54.65

Gene sequence:

>882_bases
ATGTTCACAGGAGTGGCGACAGCATTAGCTACCCCGTTTCAAGAAAATGGGCAGTTGAATCTAGAAGCATGGGCAGCATT
AATTGAAGACCAAATCAAAGAAGGCGTGACGGGTCTCGTCATCGGCGGGACGACCGGCGAAGGGATGACAATCACTGATG
ATGAGTTTGAGACATTGCTCGTTCGAGCGGTGGAGGTCGCAAACGGTCGAGCCGTGATCATCGCGGGGACCGGGTCGAAC
AATACAGCAGTCAGTATCGAAAAAACGAAACGGGCCGCCGAACTTGGTGCGGAGATGGCGATGGTCGTCACACCATACTA
TAACAAGTCGACACAGGCCGGGCTGATTGCTCACTTTACCGCGATTGCGGATGCGTCACCGATTCCACTCATGCTCTATA
ATGTTCCGTCACGGACGGGTGTCGCCTTGGCACCGGAAACGGTCGGTGAGCTCGCAGAACATCCACGTATCACGGCCTTG
AAAGAAGCGAGTGGGGATATCTCGGTCATGGCGCAGATGATGGCCCATATTCCGGAGGGGTTCACTGTTTACTGTGGAAA
TGACGATCAAATCTTGCCATACATGGCTTGGGGCGCACAAGGTGTCGTCTCCGTTTTATCAAACGTCTATCCGGGAGCGA
CGGTCGCGTTGGCAGAGGCGCTGTTGGCAGGTGATTTACAGACGGCTCGCACGTGGCAAATTCGCTTGCTCCCAGTCATC
GATGAACTCTTCGCTGAAGTGAATCCGATTCCGGTGAAGGCGGCGCTTCAAGCGCGAGGTTTTGAAGTCGGCGCGCCACG
CCTACCGCTCGTTCCGATGAGCGCGACGGCTGAAGCGAAATTACTGTCGGCGATGGAGCAATTCAATGAGGTGAGACAAT
GA

Upstream 100 bases:

>100_bases
GTGCGTTGCAGATAACGTCCGAAAAGGAGCGGCCGCGAATGCCGTTCAAATCGCACAATGTATGATTAGTCAAACAGCGA
CGAAATAAGGAGGAGATGAC

Downstream 100 bases:

>100_bases
ATGTTCTCATCCATGGGTTTGGCGCAATGGGACGGATTGTCGAAGAGGTCGCTCATGCGCAAGGTGTGAATGTCACGGCA
ATCGTCTCACCGGGAAGTGA

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MFTGVATALATPFQENGQLNLEAWAALIEDQIKEGVTGLVIGGTTGEGMTITDDEFETLLVRAVEVANGRAVIIAGTGSN
NTAVSIEKTKRAAELGAEMAMVVTPYYNKSTQAGLIAHFTAIADASPIPLMLYNVPSRTGVALAPETVGELAEHPRITAL
KEASGDISVMAQMMAHIPEGFTVYCGNDDQILPYMAWGAQGVVSVLSNVYPGATVALAEALLAGDLQTARTWQIRLLPVI
DELFAEVNPIPVKAALQARGFEVGAPRLPLVPMSATAEAKLLSAMEQFNEVRQ

Sequences:

>Translated_293_residues
MFTGVATALATPFQENGQLNLEAWAALIEDQIKEGVTGLVIGGTTGEGMTITDDEFETLLVRAVEVANGRAVIIAGTGSN
NTAVSIEKTKRAAELGAEMAMVVTPYYNKSTQAGLIAHFTAIADASPIPLMLYNVPSRTGVALAPETVGELAEHPRITAL
KEASGDISVMAQMMAHIPEGFTVYCGNDDQILPYMAWGAQGVVSVLSNVYPGATVALAEALLAGDLQTARTWQIRLLPVI
DELFAEVNPIPVKAALQARGFEVGAPRLPLVPMSATAEAKLLSAMEQFNEVRQ
>Mature_293_residues
MFTGVATALATPFQENGQLNLEAWAALIEDQIKEGVTGLVIGGTTGEGMTITDDEFETLLVRAVEVANGRAVIIAGTGSN
NTAVSIEKTKRAAELGAEMAMVVTPYYNKSTQAGLIAHFTAIADASPIPLMLYNVPSRTGVALAPETVGELAEHPRITAL
KEASGDISVMAQMMAHIPEGFTVYCGNDDQILPYMAWGAQGVVSVLSNVYPGATVALAEALLAGDLQTARTWQIRLLPVI
DELFAEVNPIPVKAALQARGFEVGAPRLPLVPMSATAEAKLLSAMEQFNEVRQ

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=284, Percent_Identity=29.5774647887324, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI13540533, Length=294, Percent_Identity=29.2517006802721, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1788823, Length=288, Percent_Identity=37.5, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1786463, Length=279, Percent_Identity=32.9749103942652, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082415, Length=285, Percent_Identity=30.1754385964912, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789620, Length=241, Percent_Identity=32.3651452282158, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_EXISA (C4L2D2)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886629.1
- ProteinModelPortal:   C4L2D2
- GeneID:   7870849
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2262
- OMA:   GMYRHFK
- ProtClustDB:   CLSK2488979
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 30952; Mature: 30952

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGVATALATPFQENGQLNLEAWAALIEDQIKEGVTGLVIGGTTGEGMTITDDEFETLL
CCCCHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECHHHHHHH
VRAVEVANGRAVIIAGTGSNNTAVSIEKTKRAAELGAEMAMVVTPYYNKSTQAGLIAHFT
HHHHHCCCCCEEEEEECCCCCEEEEEHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHH
AIADASPIPLMLYNVPSRTGVALAPETVGELAEHPRITALKEASGDISVMAQMMAHIPEG
HHCCCCCCCEEEEECCCCCCEEECHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCC
FTVYCGNDDQILPYMAWGAQGVVSVLSNVYPGATVALAEALLAGDLQTARTWQIRLLPVI
CEEEECCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHHH
DELFAEVNPIPVKAALQARGFEVGAPRLPLVPMSATAEAKLLSAMEQFNEVRQ
HHHHHHCCCCCCHHHHHHCCEECCCCCCCEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTGVATALATPFQENGQLNLEAWAALIEDQIKEGVTGLVIGGTTGEGMTITDDEFETLL
CCCCHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECHHHHHHH
VRAVEVANGRAVIIAGTGSNNTAVSIEKTKRAAELGAEMAMVVTPYYNKSTQAGLIAHFT
HHHHHCCCCCEEEEEECCCCCEEEEEHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHH
AIADASPIPLMLYNVPSRTGVALAPETVGELAEHPRITALKEASGDISVMAQMMAHIPEG
HHCCCCCCCEEEEECCCCCCEEECHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCC
FTVYCGNDDQILPYMAWGAQGVVSVLSNVYPGATVALAEALLAGDLQTARTWQIRLLPVI
CEEEECCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHHH
DELFAEVNPIPVKAALQARGFEVGAPRLPLVPMSATAEAKLLSAMEQFNEVRQ
HHHHHHCCCCCCHHHHHHCCEECCCCCCCEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA