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The map label for this gene is lysC [H]

Identifier: 229917981

GI number: 229917981

Start: 2214223

End: 2215440

Strand: Direct

Name: lysC [H]

Synonym: EAT1b_2260

Alternate gene names: 229917981

Gene position: 2214223-2215440 (Clockwise)

Preceding gene: 229917980

Following gene: 229917982

Centisome position: 73.81

GC content: 51.64

Gene sequence:

>1218_bases
TTGAAAGTGGGGAACTTGATGACAACCGTACTGAAATTTGGTGGCAGCTCCGTTGCCACAATCGAACAAATACAAGCCAT
TTCGGACTATTTACAGACACGTGTGGCGACCGGTGAAAAATTGGTCGTCGTCGTATCGGCGATGGGCAAAACGACGGATT
CGCTCCTGTCGCTCGCCAAACAAATCACGGATCGCCCGATGATTCGTGAACTCGATCGGCTACTCGCCGTCGGGGAAGAA
CAGACAATCAGCCTACTCAGTATCGCCTTGAACTCACGAGGCGTGCCGGCCATCTCTCTAACGGGACAGCAAGCCGGGAT
CGATACGATGGGCATTCATACGAAGAGCAAAATCAAGGCGATTCGGAAAGAAGTGTTACACGAGAAGTTGCGTCAATACG
ACGTCCTGATTGTCGCTGGATTCCAAGGCGTAAATGAAGCGGGAGATGTCACGACCCTTGGGCGTGGCGGTTCGGACACG
ACAGCCGTCGCACTGGCGGCCGTCCTCAGTCAGCGTTGTGAAATTTATACGGACGTCGCGGGTGTCTATACGACAGACCC
ACGAATCCATCCGACGGCGAAACGTCTGGACACCGTCTCTTATGACGAAATGATGGAGATGAGCGCACTGGGCTCTAAAG
TGATGGAGATGCGCAGCGTGGAGCTCGCGAAAAAGTACGACGTCAATATTTTTGTTGGGAAGACGCTCTCGAGCGAAGGA
GGAACATGGATCATGGATACGACGCAAGCGATGGAACAAAAAGCAGTCACGAGTGTGAGTGTCGTCAAGAACGTACTCAG
TGTCTCAATCAAACATATTCCGCATACGGCGGCGGGAGTCGCAGACATCTTTGAACGATTGTCAGAGCGACACGTCAACA
TCGATATGATTTCACAGACGGCATTCGACGGTGAAGTGTTCCTCTCATTCACATGTCCGCTCGATGAAGAGGCATTTTTA
GAAGAGGCACTCGCGGAGTTGACAGCGACCTTGCCTCAAATCAAAATCGATCGTCACACGGAACATGCAAAAATTTCGGT
CGTCGGAATCGGCATGCGAGACGCGACAGGCGTCGCATCAGAGCTCTTCGCGACATTCCGCGAGATGGGGATTCCATTCT
ATCAAGTCACGACATCAGAAATCAGTATTTCCTATACGATTCAAGAACAAGATGTGGAAGCGGCAGTCGCAGCCATTGCG
GCCCGGTTCAACTTATAA

Upstream 100 bases:

>100_bases
CGGAACGATTCTTTAATCGTTTTTCAACTGCGCGTACAAGCGGCAGACCGTAGGGAGAGTGCTTCCTACCGGTCTGCCGC
TTTTTCTGTTGGTGGACATC

Downstream 100 bases:

>100_bases
GGAGGTAGCACGATGTATAACGTAGCAGTCGTCGGTGCAACAGGTGCCGTCGGACAAAAAATGATTGACGTCTTAACGGA
ATATGAATTTCCAATCAGCG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKVGNLMTTVLKFGGSSVATIEQIQAISDYLQTRVATGEKLVVVVSAMGKTTDSLLSLAKQITDRPMIRELDRLLAVGEE
QTISLLSIALNSRGVPAISLTGQQAGIDTMGIHTKSKIKAIRKEVLHEKLRQYDVLIVAGFQGVNEAGDVTTLGRGGSDT
TAVALAAVLSQRCEIYTDVAGVYTTDPRIHPTAKRLDTVSYDEMMEMSALGSKVMEMRSVELAKKYDVNIFVGKTLSSEG
GTWIMDTTQAMEQKAVTSVSVVKNVLSVSIKHIPHTAAGVADIFERLSERHVNIDMISQTAFDGEVFLSFTCPLDEEAFL
EEALAELTATLPQIKIDRHTEHAKISVVGIGMRDATGVASELFATFREMGIPFYQVTTSEISISYTIQEQDVEAAVAAIA
ARFNL

Sequences:

>Translated_405_residues
MKVGNLMTTVLKFGGSSVATIEQIQAISDYLQTRVATGEKLVVVVSAMGKTTDSLLSLAKQITDRPMIRELDRLLAVGEE
QTISLLSIALNSRGVPAISLTGQQAGIDTMGIHTKSKIKAIRKEVLHEKLRQYDVLIVAGFQGVNEAGDVTTLGRGGSDT
TAVALAAVLSQRCEIYTDVAGVYTTDPRIHPTAKRLDTVSYDEMMEMSALGSKVMEMRSVELAKKYDVNIFVGKTLSSEG
GTWIMDTTQAMEQKAVTSVSVVKNVLSVSIKHIPHTAAGVADIFERLSERHVNIDMISQTAFDGEVFLSFTCPLDEEAFL
EEALAELTATLPQIKIDRHTEHAKISVVGIGMRDATGVASELFATFREMGIPFYQVTTSEISISYTIQEQDVEAAVAAIA
ARFNL
>Mature_405_residues
MKVGNLMTTVLKFGGSSVATIEQIQAISDYLQTRVATGEKLVVVVSAMGKTTDSLLSLAKQITDRPMIRELDRLLAVGEE
QTISLLSIALNSRGVPAISLTGQQAGIDTMGIHTKSKIKAIRKEVLHEKLRQYDVLIVAGFQGVNEAGDVTTLGRGGSDT
TAVALAAVLSQRCEIYTDVAGVYTTDPRIHPTAKRLDTVSYDEMMEMSALGSKVMEMRSVELAKKYDVNIFVGKTLSSEG
GTWIMDTTQAMEQKAVTSVSVVKNVLSVSIKHIPHTAAGVADIFERLSERHVNIDMISQTAFDGEVFLSFTCPLDEEAFL
EEALAELTATLPQIKIDRHTEHAKISVVGIGMRDATGVASELFATFREMGIPFYQVTTSEISISYTIQEQDVEAAVAAIA
ARFNL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=462, Percent_Identity=25.974025974026, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790455, Length=266, Percent_Identity=28.1954887218045, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790376, Length=283, Percent_Identity=25.0883392226148, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=373, Percent_Identity=23.8605898123324, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43883; Mature: 43883

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGNLMTTVLKFGGSSVATIEQIQAISDYLQTRVATGEKLVVVVSAMGKTTDSLLSLAK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCCCHHHHHHHHH
QITDRPMIRELDRLLAVGEEQTISLLSIALNSRGVPAISLTGQQAGIDTMGIHTKSKIKA
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHH
IRKEVLHEKLRQYDVLIVAGFQGVNEAGDVTTLGRGGSDTTAVALAAVLSQRCEIYTDVA
HHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
GVYTTDPRIHPTAKRLDTVSYDEMMEMSALGSKVMEMRSVELAKKYDVNIFVGKTLSSEG
CEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEECCCC
GTWIMDTTQAMEQKAVTSVSVVKNVLSVSIKHIPHTAAGVADIFERLSERHVNIDMISQT
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECC
AFDGEVFLSFTCPLDEEAFLEEALAELTATLPQIKIDRHTEHAKISVVGIGMRDATGVAS
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEECCCCHHHHHH
ELFATFREMGIPFYQVTTSEISISYTIQEQDVEAAVAAIAARFNL
HHHHHHHHHCCCEEEEEECEEEEEEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVGNLMTTVLKFGGSSVATIEQIQAISDYLQTRVATGEKLVVVVSAMGKTTDSLLSLAK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCCCHHHHHHHHH
QITDRPMIRELDRLLAVGEEQTISLLSIALNSRGVPAISLTGQQAGIDTMGIHTKSKIKA
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHH
IRKEVLHEKLRQYDVLIVAGFQGVNEAGDVTTLGRGGSDTTAVALAAVLSQRCEIYTDVA
HHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
GVYTTDPRIHPTAKRLDTVSYDEMMEMSALGSKVMEMRSVELAKKYDVNIFVGKTLSSEG
CEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEECCCC
GTWIMDTTQAMEQKAVTSVSVVKNVLSVSIKHIPHTAAGVADIFERLSERHVNIDMISQT
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECC
AFDGEVFLSFTCPLDEEAFLEEALAELTATLPQIKIDRHTEHAKISVVGIGMRDATGVAS
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEECCCCHHHHHH
ELFATFREMGIPFYQVTTSEISISYTIQEQDVEAAVAAIAARFNL
HHHHHHHHHCCCEEEEEECEEEEEEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]