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The map label for this gene is alaXL [H]

Identifier: 229917979

GI number: 229917979

Start: 2212287

End: 2213447

Strand: Direct

Name: alaXL [H]

Synonym: EAT1b_2258

Alternate gene names: 229917979

Gene position: 2212287-2213447 (Clockwise)

Preceding gene: 229917977

Following gene: 229917980

Centisome position: 73.75

GC content: 50.47

Gene sequence:

>1161_bases
ATGGAACCATATACAGCAAGTACAGTCGTGACCTCGATAAAGGAAGACGGCAACGGTCATTGGCACACACTCGAGAACAG
TCCGTTTTATGCGGAAGGTGGGGGACAACCGGCTGACCGTGGATGGATTGGGACGCTTCCTGTACTAGATGTACAAATCA
AGGACGGGGAAGTGTGGCATCTCTTGTCAGAATCATTACCAACGGGAAAAGCGGTAGAGGTTCGAATCGATGAGGATGTC
AGACGGGATCATTCCATCCAGCATACGGCTCAACATGTGATAACGGCGATTTTAGAAGACCGTCATGGCATTCGGACGCT
CAGCTTTGGAATCGGAGAAACGGATTCATCCATTGAGATTGAAACGGCAACGATGGACTGGGCGCACGTTGAGGCACTTG
AACAAGAGATGCAACAACTCATCTTTGAAAACCGTCCGATTCGTGTCTATGAAATCCCAAAAGAAGAAGTAGACCGGGCG
CGCTTACGGAAAGAGACGGACCGAACGGGGATGATTCGCATCGTCGAAATCGAAGGTCTAGATTACAATGCATGTGGTGG
CACTCATGTGGAGACAACGGGACAACTCGGTACCATCCATGTGACGGCGATGAAAAAAGTGAGGGGGAATCTCCGCTTGA
CGTATGTGGCGGGAAATCGAGCGATCAAGGCGACTCAACAAGCGCGAGCAGCGTTGCGGACGATTCACCATACCTTCTCG
ACAACGAATGAATCCGTTGCAGCCCGTGTAATCGAAGAACATACGATTCGTTTGGAACGTGAAAAAGAAATTAAAACGCT
CGAAGAACGGATTGCCAAAGCGAAAGTAGAGGCCGTGTTGAAAGAAGATAACTATGTGGCCTTACATGTCGGAAGAACAG
AAGCGGAGACGTTGACGACCGCCATCATCGAACGAATCGCGGCAACAGGGCGAATTGCCGTCGGGGCCAACTATGGAACG
CAGAAGTTGTTCGTCATGCATGACGGGACACAGGATATCGCCGTCGGCACATTCTTGAAAGCGGCGCTCCAAGAGATGCA
GATCGGTCGTGGCGGTGGAAACCATAAGCAGGCTCAAGCGCGTTTCACATCCGTAGCGGAACTAGAAAAAGGAATCGAAG
AAATCGTGAAACGACTGCAGGAAGGGGTTTTACCATGTTGA

Upstream 100 bases:

>100_bases
AACATATCATTTCGCTCCTGACTGAGAAGATGTATGTTTCATTATAGACGAAATTAAACTAGTTGTCTGAACAGATTATA
GAGAGAGGGTGGAGGACAAG

Downstream 100 bases:

>100_bases
TCATTCAATCGGGAAATGAAGCAACGTTGATTGAACAGGCCAAACAAGTGCGGGAAATCGTATTCGTTCAGGAACAAGGG
ATCGATGCGAGTTTAGAATA

Product: Threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MEPYTASTVVTSIKEDGNGHWHTLENSPFYAEGGGQPADRGWIGTLPVLDVQIKDGEVWHLLSESLPTGKAVEVRIDEDV
RRDHSIQHTAQHVITAILEDRHGIRTLSFGIGETDSSIEIETATMDWAHVEALEQEMQQLIFENRPIRVYEIPKEEVDRA
RLRKETDRTGMIRIVEIEGLDYNACGGTHVETTGQLGTIHVTAMKKVRGNLRLTYVAGNRAIKATQQARAALRTIHHTFS
TTNESVAARVIEEHTIRLEREKEIKTLEERIAKAKVEAVLKEDNYVALHVGRTEAETLTTAIIERIAATGRIAVGANYGT
QKLFVMHDGTQDIAVGTFLKAALQEMQIGRGGGNHKQAQARFTSVAELEKGIEEIVKRLQEGVLPC

Sequences:

>Translated_386_residues
MEPYTASTVVTSIKEDGNGHWHTLENSPFYAEGGGQPADRGWIGTLPVLDVQIKDGEVWHLLSESLPTGKAVEVRIDEDV
RRDHSIQHTAQHVITAILEDRHGIRTLSFGIGETDSSIEIETATMDWAHVEALEQEMQQLIFENRPIRVYEIPKEEVDRA
RLRKETDRTGMIRIVEIEGLDYNACGGTHVETTGQLGTIHVTAMKKVRGNLRLTYVAGNRAIKATQQARAALRTIHHTFS
TTNESVAARVIEEHTIRLEREKEIKTLEERIAKAKVEAVLKEDNYVALHVGRTEAETLTTAIIERIAATGRIAVGANYGT
QKLFVMHDGTQDIAVGTFLKAALQEMQIGRGGGNHKQAQARFTSVAELEKGIEEIVKRLQEGVLPC
>Mature_386_residues
MEPYTASTVVTSIKEDGNGHWHTLENSPFYAEGGGQPADRGWIGTLPVLDVQIKDGEVWHLLSESLPTGKAVEVRIDEDV
RRDHSIQHTAQHVITAILEDRHGIRTLSFGIGETDSSIEIETATMDWAHVEALEQEMQQLIFENRPIRVYEIPKEEVDRA
RLRKETDRTGMIRIVEIEGLDYNACGGTHVETTGQLGTIHVTAMKKVRGNLRLTYVAGNRAIKATQQARAALRTIHHTFS
TTNESVAARVIEEHTIRLEREKEIKTLEERIAKAKVEAVLKEDNYVALHVGRTEAETLTTAIIERIAATGRIAVGANYGT
QKLFVMHDGTQDIAVGTFLKAALQEMQIGRGGGNHKQAQARFTSVAELEKGIEEIVKRLQEGVLPC

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=261, Percent_Identity=32.5670498084291, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI217416402, Length=261, Percent_Identity=32.5670498084291, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1789048, Length=377, Percent_Identity=25.4641909814324, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324288, Length=287, Percent_Identity=26.8292682926829, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18543305, Length=206, Percent_Identity=33.495145631068, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 42916; Mature: 42916

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPYTASTVVTSIKEDGNGHWHTLENSPFYAEGGGQPADRGWIGTLPVLDVQIKDGEVWH
CCCCCHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCCEECCCEEEEEECCCCCHH
LLSESLPTGKAVEVRIDEDVRRDHSIQHTAQHVITAILEDRHGIRTLSFGIGETDSSIEI
HHHHCCCCCCEEEEEECHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEE
ETATMDWAHVEALEQEMQQLIFENRPIRVYEIPKEEVDRARLRKETDRTGMIRIVEIEGL
EEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECC
DYNACGGTHVETTGQLGTIHVTAMKKVRGNLRLTYVAGNRAIKATQQARAALRTIHHTFS
CCCCCCCCEEECCCCEEEEEEEEEHHHCCCEEEEEEECCCEEHHHHHHHHHHHHHHHHHC
TTNESVAARVIEEHTIRLEREKEIKTLEERIAKAKVEAVLKEDNYVALHVGRTEAETLTT
CCCHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
AIIERIAATGRIAVGANYGTQKLFVMHDGTQDIAVGTFLKAALQEMQIGRGGGNHKQAQA
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
RFTSVAELEKGIEEIVKRLQEGVLPC
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEPYTASTVVTSIKEDGNGHWHTLENSPFYAEGGGQPADRGWIGTLPVLDVQIKDGEVWH
CCCCCHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCCEECCCEEEEEECCCCCHH
LLSESLPTGKAVEVRIDEDVRRDHSIQHTAQHVITAILEDRHGIRTLSFGIGETDSSIEI
HHHHCCCCCCEEEEEECHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEE
ETATMDWAHVEALEQEMQQLIFENRPIRVYEIPKEEVDRARLRKETDRTGMIRIVEIEGL
EEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECC
DYNACGGTHVETTGQLGTIHVTAMKKVRGNLRLTYVAGNRAIKATQQARAALRTIHHTFS
CCCCCCCCEEECCCCEEEEEEEEEHHHCCCEEEEEEECCCEEHHHHHHHHHHHHHHHHHC
TTNESVAARVIEEHTIRLEREKEIKTLEERIAKAKVEAVLKEDNYVALHVGRTEAETLTT
CCCHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
AIIERIAATGRIAVGANYGTQKLFVMHDGTQDIAVGTFLKAALQEMQIGRGGGNHKQAQA
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
RFTSVAELEKGIEEIVKRLQEGVLPC
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]