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The map label for this gene is purR [H]

Identifier: 229917901

GI number: 229917901

Start: 2115832

End: 2116845

Strand: Direct

Name: purR [H]

Synonym: EAT1b_2179

Alternate gene names: 229917901

Gene position: 2115832-2116845 (Clockwise)

Preceding gene: 229917900

Following gene: 229917902

Centisome position: 70.53

GC content: 50.3

Gene sequence:

>1014_bases
ATGGGTACGATTTATGATTTAGCCAAAAAAACAGGATTCTCAATCACGACAGTGTCCAAAGCGTTGAACAATTATAAAGA
CGTGAGTGACAAGACGAGAGCGAAGATTCTTCAAGCCGCTGCTGAGATGGACTATTTGCCGAACGCACATGCCCAGTCGT
TATCGACGAAAAAATCATGGGCGATTGGGGTCATGTTCTCAGAAGCGAATGAAGTCGGGATGAAGCACCCGTTCTTTAAC
GGAATCATCGAAAGTTTTCGTCACGCGACAGAGGAGCAGGGCTACGATTTGATTTTCGCCTCGCGGAATCTGCGCAATCG
TGACATGAGTTACCTCGAACATTTTAAACACCGTGCGGTCGACGGCATCGTCGTCATCTGCTCGGACCGAATGGATGAGC
AAGTTCAAGAGCTCATGCGAAGCGACATCCCGATCGTCGTCGTGGATATGGACAGCGCTAACTGTAGCGTCGTCTATTCC
GACAACATCGAAGGAGCTCGTCTCGCCGTGAATTATCTCTATGAACTCGGTCACCGTAAAATTGCCCACATCGCTGGGGA
TGAGACGATTGACGCTGGAGCGGCACGGATCAAAGGATATGAACTTGCGATGCAAGAGCTGGACCTTCCGATTCGACCGG
GCTACCTCGTCAACGCCGGTTTCTTCTCGGTTGAGGAAGGCAAGCAAGCGATGGAACGATTGCTTGAACTAGACGATCAA
CCGACTGCAGTCTTTGTGGCCGGGGATCAAATGGCAATCGGTGTCATCGAAGCCATTCACGAACACGGTCTTCACGTCCC
GAATGACATCTCTGTCATCGGATATGATGACATTGAAATGATTCAATACATCACGCCGAAACTGACCACGATTCGCCAAG
ATACGGACGAAATCGGGGAAGCGGCAGCAGAACTTTTACTTGAACAAATGACCGCGAAAGAGCGACTCACTGAACGTCGC
GTCATTCCGGTACGCTTAGTCGAACGGGCATCCTGTGCACCGGTCAAACAGTGA

Upstream 100 bases:

>100_bases
AAAAAAGAATCAAAAAGTTTCCTCGAAAAAGAATTGATTCCTACAAATCCATACGCTACAATCAAATTACCGAAAGCGCT
TTCGGGAAGAGGAGAATGAA

Downstream 100 bases:

>100_bases
TTTATAGCCAATGATATTTCGAAGTCGCTCTCTTTTCTTCAGTGGATGGAGAGGGGAGCGCGATTTCGAAAGTCATTTTT
AGAGAAAATCGAAACCGCTT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MGTIYDLAKKTGFSITTVSKALNNYKDVSDKTRAKILQAAAEMDYLPNAHAQSLSTKKSWAIGVMFSEANEVGMKHPFFN
GIIESFRHATEEQGYDLIFASRNLRNRDMSYLEHFKHRAVDGIVVICSDRMDEQVQELMRSDIPIVVVDMDSANCSVVYS
DNIEGARLAVNYLYELGHRKIAHIAGDETIDAGAARIKGYELAMQELDLPIRPGYLVNAGFFSVEEGKQAMERLLELDDQ
PTAVFVAGDQMAIGVIEAIHEHGLHVPNDISVIGYDDIEMIQYITPKLTTIRQDTDEIGEAAAELLLEQMTAKERLTERR
VIPVRLVERASCAPVKQ

Sequences:

>Translated_337_residues
MGTIYDLAKKTGFSITTVSKALNNYKDVSDKTRAKILQAAAEMDYLPNAHAQSLSTKKSWAIGVMFSEANEVGMKHPFFN
GIIESFRHATEEQGYDLIFASRNLRNRDMSYLEHFKHRAVDGIVVICSDRMDEQVQELMRSDIPIVVVDMDSANCSVVYS
DNIEGARLAVNYLYELGHRKIAHIAGDETIDAGAARIKGYELAMQELDLPIRPGYLVNAGFFSVEEGKQAMERLLELDDQ
PTAVFVAGDQMAIGVIEAIHEHGLHVPNDISVIGYDDIEMIQYITPKLTTIRQDTDEIGEAAAELLLEQMTAKERLTERR
VIPVRLVERASCAPVKQ
>Mature_336_residues
GTIYDLAKKTGFSITTVSKALNNYKDVSDKTRAKILQAAAEMDYLPNAHAQSLSTKKSWAIGVMFSEANEVGMKHPFFNG
IIESFRHATEEQGYDLIFASRNLRNRDMSYLEHFKHRAVDGIVVICSDRMDEQVQELMRSDIPIVVVDMDSANCSVVYSD
NIEGARLAVNYLYELGHRKIAHIAGDETIDAGAARIKGYELAMQELDLPIRPGYLVNAGFFSVEEGKQAMERLLELDDQP
TAVFVAGDQMAIGVIEAIHEHGLHVPNDISVIGYDDIEMIQYITPKLTTIRQDTDEIGEAAAELLLEQMTAKERLTERRV
IPVRLVERASCAPVKQ

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=35.5029585798817, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=33.6309523809524, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=33.4328358208955, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1788474, Length=314, Percent_Identity=33.1210191082803, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1786540, Length=342, Percent_Identity=31.8713450292398, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=27.6190476190476, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=27.7027027027027, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789068, Length=305, Percent_Identity=26.8852459016393, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI48994940, Length=316, Percent_Identity=28.1645569620253, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789456, Length=341, Percent_Identity=28.1524926686217, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786268, Length=313, Percent_Identity=23.0031948881789, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790715, Length=314, Percent_Identity=24.8407643312102, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787906, Length=347, Percent_Identity=22.1902017291066, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=22.7272727272727, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37653; Mature: 37522

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTIYDLAKKTGFSITTVSKALNNYKDVSDKTRAKILQAAAEMDYLPNAHAQSLSTKKSW
CCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCE
AIGVMFSEANEVGMKHPFFNGIIESFRHATEEQGYDLIFASRNLRNRDMSYLEHFKHRAV
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCC
DGIVVICSDRMDEQVQELMRSDIPIVVVDMDSANCSVVYSDNIEGARLAVNYLYELGHRK
CCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCHH
IAHIAGDETIDAGAARIKGYELAMQELDLPIRPGYLVNAGFFSVEEGKQAMERLLELDDQ
EEHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEHHHHHHHHHHHHCCCCC
PTAVFVAGDQMAIGVIEAIHEHGLHVPNDISVIGYDDIEMIQYITPKLTTIRQDTDEIGE
CCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHCCHHHHHH
AAAELLLEQMTAKERLTERRVIPVRLVERASCAPVKQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GTIYDLAKKTGFSITTVSKALNNYKDVSDKTRAKILQAAAEMDYLPNAHAQSLSTKKSW
CCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCE
AIGVMFSEANEVGMKHPFFNGIIESFRHATEEQGYDLIFASRNLRNRDMSYLEHFKHRAV
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCC
DGIVVICSDRMDEQVQELMRSDIPIVVVDMDSANCSVVYSDNIEGARLAVNYLYELGHRK
CCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCHH
IAHIAGDETIDAGAARIKGYELAMQELDLPIRPGYLVNAGFFSVEEGKQAMERLLELDDQ
EEHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEHHHHHHHHHHHHCCCCC
PTAVFVAGDQMAIGVIEAIHEHGLHVPNDISVIGYDDIEMIQYITPKLTTIRQDTDEIGE
CCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHCCHHHHHH
AAAELLLEQMTAKERLTERRVIPVRLVERASCAPVKQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA