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The map label for this gene is argF [H]

Identifier: 229917792

GI number: 229917792

Start: 2022334

End: 2023260

Strand: Direct

Name: argF [H]

Synonym: EAT1b_2070

Alternate gene names: 229917792

Gene position: 2022334-2023260 (Clockwise)

Preceding gene: 229917791

Following gene: 229917794

Centisome position: 67.41

GC content: 49.62

Gene sequence:

>927_bases
ATGACAGTGAAGACGATACAACATTTTTTAACGATGGAGGAATTGACACCGGACACGCTCGATTCGTTGATGCAATTGGC
ACACGAAGTGAAACGCTCACCTCAATCTTATGCAACGGCATTGACAGGAAAGAAAGTCGCCTTGCTATTTGAAAAGGCAT
CGACACGGACACGTATGTCATTTGAAGTCGGGGTCGTCGAATTAGGTGGATACCCACTCTTTCTAAGCGGGCAAGATTTG
CAAATCGGGCGTGGGGAGCCGTTGACGGATACGATTCAAGTGATGAGTCGGTATGTGGACGCACTCATGATTCGGACGTA
TGCGCATGAAACGGTGGAGACGTTGGCACAACACGGGTCAATCCCAATCATCAATGGATTGACGGACTTGCATCACCCGT
GCCAAGTGCTCGCGGATTTATTGACGATTGAAGAACAGTTCGGGAGTCGTGCCGGAAAAGTGTTGGCGTATATCGGAGAC
GGCAATAATATGGCACACTCGCTTATGATCGGTGGGGCGCTGAGTGGGATGCATATCCGCATCGCCTCACCAAACGGATA
TACCGTTGATCATGCCATTTATGAGAAAGCGCAGAAGTTGGCTACATCGACCGGAGGAAGCATTGCATTGTTCGAAAACC
CATCGCTTGCTGCGAACGAGGCGGATATTGTCTACACGGATGTCTTTGCGAGCATGGGACAGGAAGGGGAAGCGGAGGAA
CGGCTGATGAAGTTTAACGGTTTTCAAGTAAATAAAGAGTTGATGGCACTTGCGAACCCCGACGCCATCTTCCTTCACTG
TTTACCGGCGCATCGCGGAGAAGAAGTGACGGCTGATGTGATTGATGGACCGCAGTCATGGGTGTTCGATCAAGCTGAGA
ATCGTCTGCATGCACAAAAAGCTCTGATGCTGACATTGTTAACGTAA

Upstream 100 bases:

>100_bases
CGTCCAAACAGTTAGCTCAGTATTATCAGATGAGTCAGTCACGGACCGGCAATCCGGTTCAGTCGTTGCAATCCATTCAA
ATGAGAGAGGGAGTAGCCAA

Downstream 100 bases:

>100_bases
CGACAAAGGGTGACACGCCACGTGTCACCCTTTTCGTTATAAGAACTGTGCCAATAATCCGAAGATGGCCAGTCCGATGA
TGATGTAGAAAAACCACTTT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTVKTIQHFLTMEELTPDTLDSLMQLAHEVKRSPQSYATALTGKKVALLFEKASTRTRMSFEVGVVELGGYPLFLSGQDL
QIGRGEPLTDTIQVMSRYVDALMIRTYAHETVETLAQHGSIPIINGLTDLHHPCQVLADLLTIEEQFGSRAGKVLAYIGD
GNNMAHSLMIGGALSGMHIRIASPNGYTVDHAIYEKAQKLATSTGGSIALFENPSLAANEADIVYTDVFASMGQEGEAEE
RLMKFNGFQVNKELMALANPDAIFLHCLPAHRGEEVTADVIDGPQSWVFDQAENRLHAQKALMLTLLT

Sequences:

>Translated_308_residues
MTVKTIQHFLTMEELTPDTLDSLMQLAHEVKRSPQSYATALTGKKVALLFEKASTRTRMSFEVGVVELGGYPLFLSGQDL
QIGRGEPLTDTIQVMSRYVDALMIRTYAHETVETLAQHGSIPIINGLTDLHHPCQVLADLLTIEEQFGSRAGKVLAYIGD
GNNMAHSLMIGGALSGMHIRIASPNGYTVDHAIYEKAQKLATSTGGSIALFENPSLAANEADIVYTDVFASMGQEGEAEE
RLMKFNGFQVNKELMALANPDAIFLHCLPAHRGEEVTADVIDGPQSWVFDQAENRLHAQKALMLTLLT
>Mature_307_residues
TVKTIQHFLTMEELTPDTLDSLMQLAHEVKRSPQSYATALTGKKVALLFEKASTRTRMSFEVGVVELGGYPLFLSGQDLQ
IGRGEPLTDTIQVMSRYVDALMIRTYAHETVETLAQHGSIPIINGLTDLHHPCQVLADLLTIEEQFGSRAGKVLAYIGDG
NNMAHSLMIGGALSGMHIRIASPNGYTVDHAIYEKAQKLATSTGGSIALFENPSLAANEADIVYTDVFASMGQEGEAEER
LMKFNGFQVNKELMALANPDAIFLHCLPAHRGEEVTADVIDGPQSWVFDQAENRLHAQKALMLTLLT

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=42.2950819672131, Blast_Score=254, Evalue=5e-68,
Organism=Homo sapiens, GI18105007, Length=306, Percent_Identity=28.4313725490196, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=40.9785932721713, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1786469, Length=326, Percent_Identity=39.8773006134969, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=29.6774193548387, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994908, Length=322, Percent_Identity=27.9503105590062, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=169, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=314, Percent_Identity=36.624203821656, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=25.4071661237785, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24642586, Length=305, Percent_Identity=26.8852459016393, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33673; Mature: 33542

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTIQHFLTMEELTPDTLDSLMQLAHEVKRSPQSYATALTGKKVALLFEKASTRTRMS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEEECCCCCEEEE
FEVGVVELGGYPLFLSGQDLQIGRGEPLTDTIQVMSRYVDALMIRTYAHETVETLAQHGS
EEEEEEEECCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPIINGLTDLHHPCQVLADLLTIEEQFGSRAGKVLAYIGDGNNMAHSLMIGGALSGMHIR
CEEECCCHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHEEECCCCCCEEEE
IASPNGYTVDHAIYEKAQKLATSTGGSIALFENPSLAANEADIVYTDVFASMGQEGEAEE
EECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCHHH
RLMKFNGFQVNKELMALANPDAIFLHCLPAHRGEEVTADVIDGPQSWVFDQAENRLHAQK
HHHHHCCEEECHHHHHHCCCCEEEEEEECCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHH
ALMLTLLT
HHHHEECC
>Mature Secondary Structure 
TVKTIQHFLTMEELTPDTLDSLMQLAHEVKRSPQSYATALTGKKVALLFEKASTRTRMS
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEEECCCCCEEEE
FEVGVVELGGYPLFLSGQDLQIGRGEPLTDTIQVMSRYVDALMIRTYAHETVETLAQHGS
EEEEEEEECCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPIINGLTDLHHPCQVLADLLTIEEQFGSRAGKVLAYIGDGNNMAHSLMIGGALSGMHIR
CEEECCCHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHEEECCCCCCEEEE
IASPNGYTVDHAIYEKAQKLATSTGGSIALFENPSLAANEADIVYTDVFASMGQEGEAEE
EECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCHHH
RLMKFNGFQVNKELMALANPDAIFLHCLPAHRGEEVTADVIDGPQSWVFDQAENRLHAQK
HHHHHCCEEECHHHHHHCCCCEEEEEEECCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHH
ALMLTLLT
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]