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The map label for this gene is narK [H]

Identifier: 229917633

GI number: 229917633

Start: 1872102

End: 1873418

Strand: Direct

Name: narK [H]

Synonym: EAT1b_1909

Alternate gene names: 229917633

Gene position: 1872102-1873418 (Clockwise)

Preceding gene: 229917631

Following gene: 229917634

Centisome position: 62.41

GC content: 53.23

Gene sequence:

>1317_bases
ATGCAACAATTAGAAAAAGGGTCACACGCACTCGCGGAGTGGAAGCCAGAGGATGTGGGATTCTGGGAAGAGACGGGAAA
ACAAGTCGCCAATCGGAACTTGCTCGTTTCGGTCCCGGCACTGACGCTTGCATTCATCGTCTGGCAAATGTGGTCGGTCG
CGGCCGTCCAATTGAATTCGATCGGGTTTAGCTTTACGCAAGGCCAGCTGTTCACGTTGGCCGCCCTACCTGGACTCGTC
GGGGCGACGTTGCGCTTTTTCTATACGTTCGCAAACAGTATATTCGGCGGTCGGAACTGGACGATCTTCTCGACATCGAT
TTTGTTGTTGCCGCTCCTAGGCATCGCCTATGCCGTTCAAAACGTCAATACGCCGTATTGGGTATTCATGATTTTGGCCG
CACTTTGCGGACTAGGCGGCGGAAACTTCTCGTCATCAAACGCCAACATCGGCGCGTTTTATCCGAAAGCGAAAAAAGGG
ACGGCGCTAGGGATTAACGGAGGCATCGGGAACTTGGGCGTCTCGCTCGTCCAATTGATCACACCGCTCGTCATCACGAC
AAGTATTTTCGGGTTCATCGGCGGGAACGCCCTCGTGACCGAGACCGGCAAAGAGATTTGGCTTCAAAACGCCGCGCTCC
TTTGGGTCGTGCCGACCATCATTTTGACGGTCTGCGCATACTTGTTCATGGATAACTTACCGACCGCGCGTCAATCGCTT
CGTGAACAGGCGAGCGTGTTTAAAGACAAACATTTTTGGATTCAAACGTTGCTCTACACGGCGGCGTTCGGTTCCTTCAT
TGGATATGCCGCAGCGTTCCCGATGATTTTAAAGTTAAAGTTCCCTGAGTCGGACTTGATGGCGCTCGCCTTCCTTGGTG
CATTCCTTGGCGCCTCTTCGCGTCCGTTCGGGGGCTGGATCAGTGATAAGATCGGAGCGGCCAAAGTGACATTAATCGTG
TTCGGAATCATGGCGCTCGGTACGATCAGTGTCATCATGGCCATCACGAACAGCAACTTGACGATGTTTTTCATCTCCTT
CGTCGTCTTGTTCATCGCAACAGGACTCAACTCTGGAGCGACGTTCGCGATGATTCCCCACGTATTCGTGGCGACTAAGA
CACCGGCCGTCGTCGGATTCTCGGCAGCGTTTGCTGCTTACGGAGCCTTCTTCATTCCGAAGATGTTCGGTTGGTCGCTC
GAGCTCGTCGGTTCGTTCAACGGTGCGTTGTTCGTCATGCTAGGGCTCTATGCGATCTCGATTGCCACCACAATTTATTA
TTACTTGCGCAGCGGTGCTGAGAAGCCGATTCGCTGA

Upstream 100 bases:

>100_bases
AGATGCGAAATAAGTGTGAAAGAAGGCACACTTTTTTTAGGGGGTGGCCGATAGGATGAGATTAGGGTACGAATTGAACC
GTTAAGAGGAGGAATAGACT

Downstream 100 bases:

>100_bases
CAAATCGACGCAGGGACTGTGAGGGTCCCTGCTGTCGTTGATGGATAGAAAGGATGAACCAAGATGACACCATGGACCGA
TCGACGGAGACGACCGTTGG

Product: major facilitator superfamily MFS_1

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQQLEKGSHALAEWKPEDVGFWEETGKQVANRNLLVSVPALTLAFIVWQMWSVAAVQLNSIGFSFTQGQLFTLAALPGLV
GATLRFFYTFANSIFGGRNWTIFSTSILLLPLLGIAYAVQNVNTPYWVFMILAALCGLGGGNFSSSNANIGAFYPKAKKG
TALGINGGIGNLGVSLVQLITPLVITTSIFGFIGGNALVTETGKEIWLQNAALLWVVPTIILTVCAYLFMDNLPTARQSL
REQASVFKDKHFWIQTLLYTAAFGSFIGYAAAFPMILKLKFPESDLMALAFLGAFLGASSRPFGGWISDKIGAAKVTLIV
FGIMALGTISVIMAITNSNLTMFFISFVVLFIATGLNSGATFAMIPHVFVATKTPAVVGFSAAFAAYGAFFIPKMFGWSL
ELVGSFNGALFVMLGLYAISIATTIYYYLRSGAEKPIR

Sequences:

>Translated_438_residues
MQQLEKGSHALAEWKPEDVGFWEETGKQVANRNLLVSVPALTLAFIVWQMWSVAAVQLNSIGFSFTQGQLFTLAALPGLV
GATLRFFYTFANSIFGGRNWTIFSTSILLLPLLGIAYAVQNVNTPYWVFMILAALCGLGGGNFSSSNANIGAFYPKAKKG
TALGINGGIGNLGVSLVQLITPLVITTSIFGFIGGNALVTETGKEIWLQNAALLWVVPTIILTVCAYLFMDNLPTARQSL
REQASVFKDKHFWIQTLLYTAAFGSFIGYAAAFPMILKLKFPESDLMALAFLGAFLGASSRPFGGWISDKIGAAKVTLIV
FGIMALGTISVIMAITNSNLTMFFISFVVLFIATGLNSGATFAMIPHVFVATKTPAVVGFSAAFAAYGAFFIPKMFGWSL
ELVGSFNGALFVMLGLYAISIATTIYYYLRSGAEKPIR
>Mature_438_residues
MQQLEKGSHALAEWKPEDVGFWEETGKQVANRNLLVSVPALTLAFIVWQMWSVAAVQLNSIGFSFTQGQLFTLAALPGLV
GATLRFFYTFANSIFGGRNWTIFSTSILLLPLLGIAYAVQNVNTPYWVFMILAALCGLGGGNFSSSNANIGAFYPKAKKG
TALGINGGIGNLGVSLVQLITPLVITTSIFGFIGGNALVTETGKEIWLQNAALLWVVPTIILTVCAYLFMDNLPTARQSL
REQASVFKDKHFWIQTLLYTAAFGSFIGYAAAFPMILKLKFPESDLMALAFLGAFLGASSRPFGGWISDKIGAAKVTLIV
FGIMALGTISVIMAITNSNLTMFFISFVVLFIATGLNSGATFAMIPHVFVATKTPAVVGFSAAFAAYGAFFIPKMFGWSL
ELVGSFNGALFVMLGLYAISIATTIYYYLRSGAEKPIR

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=456, Percent_Identity=43.640350877193, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1787743, Length=454, Percent_Identity=44.273127753304, Blast_Score=324, Evalue=7e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47167; Mature: 47167

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLEKGSHALAEWKPEDVGFWEETGKQVANRNLLVSVPALTLAFIVWQMWSVAAVQLNS
CCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
IGFSFTQGQLFTLAALPGLVGATLRFFYTFANSIFGGRNWTIFSTSILLLPLLGIAYAVQ
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
NVNTPYWVFMILAALCGLGGGNFSSSNANIGAFYPKAKKGTALGINGGIGNLGVSLVQLI
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHH
TPLVITTSIFGFIGGNALVTETGKEIWLQNAALLWVVPTIILTVCAYLFMDNLPTARQSL
HHHHHHHHHHHHHCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
REQASVFKDKHFWIQTLLYTAAFGSFIGYAAAFPMILKLKFPESDLMALAFLGAFLGASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCC
RPFGGWISDKIGAAKVTLIVFGIMALGTISVIMAITNSNLTMFFISFVVLFIATGLNSGA
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
TFAMIPHVFVATKTPAVVGFSAAFAAYGAFFIPKMFGWSLELVGSFNGALFVMLGLYAIS
EEEECCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
IATTIYYYLRSGAEKPIR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQQLEKGSHALAEWKPEDVGFWEETGKQVANRNLLVSVPALTLAFIVWQMWSVAAVQLNS
CCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
IGFSFTQGQLFTLAALPGLVGATLRFFYTFANSIFGGRNWTIFSTSILLLPLLGIAYAVQ
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
NVNTPYWVFMILAALCGLGGGNFSSSNANIGAFYPKAKKGTALGINGGIGNLGVSLVQLI
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHH
TPLVITTSIFGFIGGNALVTETGKEIWLQNAALLWVVPTIILTVCAYLFMDNLPTARQSL
HHHHHHHHHHHHHCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
REQASVFKDKHFWIQTLLYTAAFGSFIGYAAAFPMILKLKFPESDLMALAFLGAFLGASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCC
RPFGGWISDKIGAAKVTLIVFGIMALGTISVIMAITNSNLTMFFISFVVLFIATGLNSGA
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
TFAMIPHVFVATKTPAVVGFSAAFAAYGAFFIPKMFGWSLELVGSFNGALFVMLGLYAIS
EEEECCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
IATTIYYYLRSGAEKPIR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]