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The map label for this gene is nagE [H]

Identifier: 229917594

GI number: 229917594

Start: 1834679

End: 1836169

Strand: Reverse

Name: nagE [H]

Synonym: EAT1b_1869

Alternate gene names: 229917594

Gene position: 1836169-1834679 (Counterclockwise)

Preceding gene: 229917595

Following gene: 229917590

Centisome position: 61.21

GC content: 51.51

Gene sequence:

>1491_bases
ATGATGAACTTTTTCCAACGTCTCGGACGTTCCCTCATGTTACCGGTCGCCGTACTACCGGCTGCTGCCATTCTCATGGG
GATTGGTTATTGGATTGACCCAGTCGGCTGGGGATCTGAAAACATTGCCGCTGCATTCTTAATTAAAGCGGGGGCCGCGA
TTATCGATAACATTCCAATTCTTTTCGCAGTCGGGGTCGCGCTCGGAATGGCTAAGAACCGTGACGGTTCAGCAGCACTC
AGTGGTCTCGTTGCCTTCTTGACGGTCACAACGCTTCTCTCTGCAGGAACGGTTGCGTTGTTCCAAGGCATCGACGTAGA
AGCCGTCAACCCGGCATTCGGGAAGATTGGGAATGCTTTTGTCGGGATTCTATCCGGTCTCGTCGCCGCTTCCATGTATA
ACCGCTTCAGTCAAGTCAAACTGCCAGATGCTCTCGCCTTCTTTAGTGGGAAGCGTCTCGTACCAATCATGACAGCCTTC
TCGATGATTCTCGTCTCGGTCGCCCTTTACTTCATCTGGCCGGTCGTCTATACAGGTCTCGTCGGATTCGGTGAATCGAT
TTCAAAACTTGGCGCATTCGGTGCCGGACTTTACGGATTCTTTAACCGTCTCTTGATTCCAACTGGTCTTCACCACGCCC
TCAACTCGGTATTCTGGTTCGACGTCGCAGGAATCAACGACATCGGAAACTTCTGGTCAGGTCAAGGTGAAAAAGGTGTG
ACAGGGATGTACCAAGCAGGTTACTTCCCAATCATGATGTTCGGTCTTCCGGCCGCAGCACTCGCGATGTACCACACAGC
GAAAACGAAACGGAAAAAACAAGCCGCTTCACTTCTTCTCGCAGCGGCATTCGCTTCATTCTTCACAGGGGTAACAGAAC
CGCTCGAGTTCTCATTCATGTTCCTCGCTCCTGTTCTTTACCTTGTACACGCAGTCCTTACAGGAATTTCGCTCTTCGTT
GCGGCAACGTTCCAATGGACAGCCGGCTTCGGGTTCAGCGCAGGCTTGGTCGACTTTATCTTGAGCTCACGCTTGCCGCT
TGCGAACCAGCCGTATATGCTCATCGTTCAAGGTCTCGTGTTCGCAGTCATTTACTACGTCATCTTCCGCTTCATGATTG
TGAAGCTCAACTTGGCAACACCAGGTCGTGAACCAGATGAAGTCGTCGATGCGGTTGCAAGTGAGACGGCAGACGGTGAA
GCGAAAGTCGCTCCGGTCGCATCAGCTTCAGGTAGCGAATACTCAGCAATGGCACATATGATTTATGACGGTCTCGGCGG
TGACAGCAACGTCACAGGCATCGAGTCGTGCATCACACGCCTACGCGTCGATGTCAAAGACATGGATACGGTCAACCAAG
ATCAAATCAAAAAAGCCGGTATTCCGGGAATCAAAGTCGTCAGTCCAAACCATATTCAAGTCATCGTCGGCACAAACGTT
CAGTTCGTCCTCGATGAGATTGAAAAAATCCGTAGCCACCGCGCCGGATAA

Upstream 100 bases:

>100_bases
AGACCTATGGCATCGTCTTGCCCGAGTCAGAACTTGGTTATATTACGCTTCACATCCAACGTCTACAAGATCAACACCAT
CAAAAAGGAGGAAATCAACA

Downstream 100 bases:

>100_bases
ACGTCGAAAGCCACCTCATTCAGGTGGCTTTTCTTGTTTGGCTGTATATTGTTTATAAAATTGAATGAAATAGTAGAGGA
AGATCCACGGTAAGATGTAC

Product: PTS system, N-acetylglucosamine-specific IIBC subunit

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MMNFFQRLGRSLMLPVAVLPAAAILMGIGYWIDPVGWGSENIAAAFLIKAGAAIIDNIPILFAVGVALGMAKNRDGSAAL
SGLVAFLTVTTLLSAGTVALFQGIDVEAVNPAFGKIGNAFVGILSGLVAASMYNRFSQVKLPDALAFFSGKRLVPIMTAF
SMILVSVALYFIWPVVYTGLVGFGESISKLGAFGAGLYGFFNRLLIPTGLHHALNSVFWFDVAGINDIGNFWSGQGEKGV
TGMYQAGYFPIMMFGLPAAALAMYHTAKTKRKKQAASLLLAAAFASFFTGVTEPLEFSFMFLAPVLYLVHAVLTGISLFV
AATFQWTAGFGFSAGLVDFILSSRLPLANQPYMLIVQGLVFAVIYYVIFRFMIVKLNLATPGREPDEVVDAVASETADGE
AKVAPVASASGSEYSAMAHMIYDGLGGDSNVTGIESCITRLRVDVKDMDTVNQDQIKKAGIPGIKVVSPNHIQVIVGTNV
QFVLDEIEKIRSHRAG

Sequences:

>Translated_496_residues
MMNFFQRLGRSLMLPVAVLPAAAILMGIGYWIDPVGWGSENIAAAFLIKAGAAIIDNIPILFAVGVALGMAKNRDGSAAL
SGLVAFLTVTTLLSAGTVALFQGIDVEAVNPAFGKIGNAFVGILSGLVAASMYNRFSQVKLPDALAFFSGKRLVPIMTAF
SMILVSVALYFIWPVVYTGLVGFGESISKLGAFGAGLYGFFNRLLIPTGLHHALNSVFWFDVAGINDIGNFWSGQGEKGV
TGMYQAGYFPIMMFGLPAAALAMYHTAKTKRKKQAASLLLAAAFASFFTGVTEPLEFSFMFLAPVLYLVHAVLTGISLFV
AATFQWTAGFGFSAGLVDFILSSRLPLANQPYMLIVQGLVFAVIYYVIFRFMIVKLNLATPGREPDEVVDAVASETADGE
AKVAPVASASGSEYSAMAHMIYDGLGGDSNVTGIESCITRLRVDVKDMDTVNQDQIKKAGIPGIKVVSPNHIQVIVGTNV
QFVLDEIEKIRSHRAG
>Mature_496_residues
MMNFFQRLGRSLMLPVAVLPAAAILMGIGYWIDPVGWGSENIAAAFLIKAGAAIIDNIPILFAVGVALGMAKNRDGSAAL
SGLVAFLTVTTLLSAGTVALFQGIDVEAVNPAFGKIGNAFVGILSGLVAASMYNRFSQVKLPDALAFFSGKRLVPIMTAF
SMILVSVALYFIWPVVYTGLVGFGESISKLGAFGAGLYGFFNRLLIPTGLHHALNSVFWFDVAGINDIGNFWSGQGEKGV
TGMYQAGYFPIMMFGLPAAALAMYHTAKTKRKKQAASLLLAAAFASFFTGVTEPLEFSFMFLAPVLYLVHAVLTGISLFV
AATFQWTAGFGFSAGLVDFILSSRLPLANQPYMLIVQGLVFAVIYYVIFRFMIVKLNLATPGREPDEVVDAVASETADGE
AKVAPVASASGSEYSAMAHMIYDGLGGDSNVTGIESCITRLRVDVKDMDTVNQDQIKKAGIPGIKVVSPNHIQVIVGTNV
QFVLDEIEKIRSHRAG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=500, Percent_Identity=42, Blast_Score=335, Evalue=6e-93,
Organism=Escherichia coli, GI1787343, Length=505, Percent_Identity=37.2277227722772, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1787908, Length=530, Percent_Identity=33.7735849056604, Blast_Score=274, Evalue=7e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 52855; Mature: 52855

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]