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The map label for this gene is ywcJ [H]

Identifier: 229917564

GI number: 229917564

Start: 1811043

End: 1811843

Strand: Reverse

Name: ywcJ [H]

Synonym: EAT1b_1839

Alternate gene names: 229917564

Gene position: 1811843-1811043 (Counterclockwise)

Preceding gene: 229917565

Following gene: 229917562

Centisome position: 60.4

GC content: 49.06

Gene sequence:

>801_bases
ATGGAAAAGCAAGTCGTCGATTATATGGAAGAAGCCGCATTTAAGAAATCATCATTGCTTCAACATTCATTTCCTCAATA
CATGTTACGATCGATAATCGCCGGTTTTTTAATCGGGATTGGAGTCGTCTTCTCATTCACCGTCGGAAACATGTTCATCG
ATGTCCCCGGTACGATGCTGATTGCAGGCGCCAGTTTCTCGGTGGCCCTCGTCTTCATTGTCTGGATGGGGGGAGAATTG
TTTACGAGCAACACGATGTATCTCTACACTGGTGCAAAACGGGGACGTGTCGCTTGGTTCGACCTCGCAAAGATATGGGG
ACTCAGTTGGGTCGGTAACTTGATCGGTGCCGTCTTGCTATCGCTTCTCGTCATCGGAGCTCACAGTATGGGTGACGTGT
CGGGTGACCATTTGCTTGCTGTCGTCGCCGCGAAGAAAATCGGTGGAGACGCCCTTGCTATTTTCTTGAAAGGAATCTTA
TGTAACGTGCTCGTCTGTTTGGCCATCTTCATGCCACTCAAAACGAACAATGATGTCGCGAAGATTATGCTGACGATGCT
TCCGGTCGTCGTATTCTTCGCTGCTGGATTCGAACACTCGATTGCGAACATGGGCGTTTTCGCTCTCGCGCTCCACTATG
TACCGTCGGACCTCGTCACCTTTAGCGGTGCCCTTTACAATCTCTTCTTCGCCACACTCGGTAATATTGTCGGTGGTGCG
TTGTTCGTTGCCGGAAGTTACTTGTATTTGAACAAATCGGAGACGGAACAAACCGTCAAGCCGATACGTCATCATGCATA
A

Upstream 100 bases:

>100_bases
GATGTGAACGGTTTCTTGAGAACTTCTTGAAAGTTTGTGAAAACAATTGCAAACTTGACGTCGACCTCCTAGACTGAAAG
GGTCTAAAGGAGGTCTTTTC

Downstream 100 bases:

>100_bases
AAAGACAGGGACCCCGATTAGCAGGGTCCCTGTTTGTTGCATTTCACGAATTATGATTTTGAAGCGTCGGCTGCTGCTGC
TTGAACAGTACCTTGTGCCA

Product: formate/nitrite transporter

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEKQVVDYMEEAAFKKSSLLQHSFPQYMLRSIIAGFLIGIGVVFSFTVGNMFIDVPGTMLIAGASFSVALVFIVWMGGEL
FTSNTMYLYTGAKRGRVAWFDLAKIWGLSWVGNLIGAVLLSLLVIGAHSMGDVSGDHLLAVVAAKKIGGDALAIFLKGIL
CNVLVCLAIFMPLKTNNDVAKIMLTMLPVVVFFAAGFEHSIANMGVFALALHYVPSDLVTFSGALYNLFFATLGNIVGGA
LFVAGSYLYLNKSETEQTVKPIRHHA

Sequences:

>Translated_266_residues
MEKQVVDYMEEAAFKKSSLLQHSFPQYMLRSIIAGFLIGIGVVFSFTVGNMFIDVPGTMLIAGASFSVALVFIVWMGGEL
FTSNTMYLYTGAKRGRVAWFDLAKIWGLSWVGNLIGAVLLSLLVIGAHSMGDVSGDHLLAVVAAKKIGGDALAIFLKGIL
CNVLVCLAIFMPLKTNNDVAKIMLTMLPVVVFFAAGFEHSIANMGVFALALHYVPSDLVTFSGALYNLFFATLGNIVGGA
LFVAGSYLYLNKSETEQTVKPIRHHA
>Mature_266_residues
MEKQVVDYMEEAAFKKSSLLQHSFPQYMLRSIIAGFLIGIGVVFSFTVGNMFIDVPGTMLIAGASFSVALVFIVWMGGEL
FTSNTMYLYTGAKRGRVAWFDLAKIWGLSWVGNLIGAVLLSLLVIGAHSMGDVSGDHLLAVVAAKKIGGDALAIFLKGIL
CNVLVCLAIFMPLKTNNDVAKIMLTMLPVVVFFAAGFEHSIANMGVFALALHYVPSDLVTFSGALYNLFFATLGNIVGGA
LFVAGSYLYLNKSETEQTVKPIRHHA

Specific function: Potential Nitrite Transporter. [C]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI87082249, Length=269, Percent_Identity=34.9442379182156, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787132, Length=268, Percent_Identity=33.955223880597, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1788837, Length=259, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321779, Length=252, Percent_Identity=31.3492063492063, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 28702; Mature: 28702

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure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HHHHCCEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377 [H]