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The map label for this gene is guaC

Identifier: 229917550

GI number: 229917550

Start: 1792022

End: 1793005

Strand: Reverse

Name: guaC

Synonym: EAT1b_1825

Alternate gene names: 229917550

Gene position: 1793005-1792022 (Counterclockwise)

Preceding gene: 229917551

Following gene: 229917544

Centisome position: 59.77

GC content: 49.09

Gene sequence:

>984_bases
ATGGATAAAGTATTTGATTATGAAGATATTCAATTAATTCCTGCAAAATGTATTGTCGACAGTCGTTCGGAATGTGATCC
GACCGTCGAGCTCGGGGGCTTCACGTTCCGTTTACCGGTCGTTCCGGCTAACATGCAGACGATTATCGATGAAAAGGTCG
CATTGATGCTGGCCAAGAACGGGTATTTTTACATCATGCATCGCTTCAATCCAGAGACGCGCCTGGCGTTTATCCAAGAC
ATGCATGAGCGTGGTTTGTATGCGTCCATCAGTGTCGGCGTGAAAGATGAAGAGTATGGGTTTATCGAAGTGCTCAAATC
GACCGGTCATACGCCAGAATTTATTACAATCGATATCGCGCATGGACACTCGAATGCGGTCATCCGAATGATTCAGCATA
TCAAACACCATCTTCCTGGATCATTCGTCATCGCGGGGAACGTCGGGACACCAGAGGCCGTTCGCGAATTAGAGCATGCC
GGAGCGGATGCGACGAAGGTTGGAATCGGTCCTGGTAAAGTGTGTATCACGAAAATCAAGACAGGTTTTGGTACCGGGGG
CTGGCAATTGGCTGCACTTCGCTGGTGTGCCAAAGCAGCGACGAAACCGATTATCGCGGATGGGGGCATCCGTACTCACG
GTGACATCGCCAAATCGATTCGCTTCGGCGCTTCGATGGTCATGATCGGTTCATTGTTCGCCGGACACGATGAATCACCG
GGGGAAACGTTCGAGCAAGACGGGAAACAACTGAAAGAGTATTTCGGTTCTGCTTCTGAGTTCCAAAAAGGAGAGCGAAA
GAACGTCGAGGGGAAAAAGATGTTTGTCGAACACAAAGGGGCATTGCAAGATACATTGACCGAGATGGAGCAAGACCTCC
AATCGGCGATTTCTTACGCGGGTGGTGACAGTCTCGAGGCATTGCGCACCGTAGACTACGTCATGGTAAAAAACTCAATC
TTCAATGGGGATAAAGTATATTAA

Upstream 100 bases:

>100_bases
AGTTATCCTTTGACTTTAGACTCTATTCACGTTATATTACCGAATGGGCGAAGTTTTTTATTGTTCAATACGCTAATGTT
CGTGAATAGGGGTGCGATAT

Downstream 100 bases:

>100_bases
AAAGGCGACCTCGTGTCGCCTTTTATAGTTCCTTCATCATCATGTAGTAATCCGATCCCGGTGGGTAGTCCGGTAGCATA
CCTGCGACTGTGTAGCCGAA

Product: guanosine 5'-monophosphate oxidoreductase

Products: NA

Alternate protein names: Guanosine 5'-monophosphate oxidoreductase; Guanosine monophosphate reductase

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MDKVFDYEDIQLIPAKCIVDSRSECDPTVELGGFTFRLPVVPANMQTIIDEKVALMLAKNGYFYIMHRFNPETRLAFIQD
MHERGLYASISVGVKDEEYGFIEVLKSTGHTPEFITIDIAHGHSNAVIRMIQHIKHHLPGSFVIAGNVGTPEAVRELEHA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAATKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHDESP
GETFEQDGKQLKEYFGSASEFQKGERKNVEGKKMFVEHKGALQDTLTEMEQDLQSAISYAGGDSLEALRTVDYVMVKNSI
FNGDKVY

Sequences:

>Translated_327_residues
MDKVFDYEDIQLIPAKCIVDSRSECDPTVELGGFTFRLPVVPANMQTIIDEKVALMLAKNGYFYIMHRFNPETRLAFIQD
MHERGLYASISVGVKDEEYGFIEVLKSTGHTPEFITIDIAHGHSNAVIRMIQHIKHHLPGSFVIAGNVGTPEAVRELEHA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAATKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHDESP
GETFEQDGKQLKEYFGSASEFQKGERKNVEGKKMFVEHKGALQDTLTEMEQDLQSAISYAGGDSLEALRTVDYVMVKNSI
FNGDKVY
>Mature_327_residues
MDKVFDYEDIQLIPAKCIVDSRSECDPTVELGGFTFRLPVVPANMQTIIDEKVALMLAKNGYFYIMHRFNPETRLAFIQD
MHERGLYASISVGVKDEEYGFIEVLKSTGHTPEFITIDIAHGHSNAVIRMIQHIKHHLPGSFVIAGNVGTPEAVRELEHA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAATKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHDESP
GETFEQDGKQLKEYFGSASEFQKGERKNVEGKKMFVEHKGALQDTLTEMEQDLQSAISYAGGDSLEALRTVDYVMVKNSI
FNGDKVY

Specific function: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI50541954, Length=344, Percent_Identity=33.1395348837209, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI50541952, Length=344, Percent_Identity=33.1395348837209, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI50541948, Length=344, Percent_Identity=33.1395348837209, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI50541956, Length=344, Percent_Identity=33.1395348837209, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI156104880, Length=344, Percent_Identity=31.3953488372093, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI217035150, Length=248, Percent_Identity=33.4677419354839, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI217035146, Length=248, Percent_Identity=33.4677419354839, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI217035148, Length=248, Percent_Identity=33.4677419354839, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI156616279, Length=248, Percent_Identity=33.4677419354839, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI34328928, Length=248, Percent_Identity=33.4677419354839, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI34328930, Length=248, Percent_Identity=33.4677419354839, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI217035152, Length=248, Percent_Identity=33.4677419354839, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI66933016, Length=248, Percent_Identity=31.8548387096774, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786293, Length=345, Percent_Identity=31.304347826087, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1788855, Length=215, Percent_Identity=38.1395348837209, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560440, Length=324, Percent_Identity=31.7901234567901, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71994385, Length=151, Percent_Identity=40.3973509933775, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994389, Length=151, Percent_Identity=40.3973509933775, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322012, Length=207, Percent_Identity=35.2657004830918, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323464, Length=203, Percent_Identity=35.4679802955665, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323585, Length=218, Percent_Identity=33.4862385321101, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28571163, Length=249, Percent_Identity=34.136546184739, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24641071, Length=249, Percent_Identity=34.136546184739, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24641073, Length=249, Percent_Identity=34.136546184739, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): GUAC_EXISA (C4L088)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886196.1
- ProteinModelPortal:   C4L088
- GeneID:   7868577
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1825
- OMA:   PDYITID
- ProtClustDB:   PRK05458
- HAMAP:   MF_01511
- InterPro:   IPR013785
- InterPro:   IPR005994
- InterPro:   IPR015875
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF036500
- TIGRFAMs:   TIGR01306

Pfam domain/function: PF00478 IMPDH

EC number: =1.7.1.7

Molecular weight: Translated: 35906; Mature: 35906

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVFDYEDIQLIPAKCIVDSRSECDPTVELGGFTFRLPVVPANMQTIIDEKVALMLAKN
CCCCCCCCCCEEECHHHEECCCCCCCCEEEECCEEEEEEECCCHHHHHHHHHHEEEEECC
GYFYIMHRFNPETRLAFIQDMHERGLYASISVGVKDEEYGFIEVLKSTGHTPEFITIDIA
CEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEE
HGHSNAVIRMIQHIKHHLPGSFVIAGNVGTPEAVRELEHAGADATKVGIGPGKVCITKIK
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEEC
TGFGTGGWQLAALRWCAKAATKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHDESP
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCC
GETFEQDGKQLKEYFGSASEFQKGERKNVEGKKMFVEHKGALQDTLTEMEQDLQSAISYA
CHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHEEEEHHCCHHHHHHHHHHHHHHHHHHHC
GGDSLEALRTVDYVMVKNSIFNGDKVY
CCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDKVFDYEDIQLIPAKCIVDSRSECDPTVELGGFTFRLPVVPANMQTIIDEKVALMLAKN
CCCCCCCCCCEEECHHHEECCCCCCCCEEEECCEEEEEEECCCHHHHHHHHHHEEEEECC
GYFYIMHRFNPETRLAFIQDMHERGLYASISVGVKDEEYGFIEVLKSTGHTPEFITIDIA
CEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEE
HGHSNAVIRMIQHIKHHLPGSFVIAGNVGTPEAVRELEHAGADATKVGIGPGKVCITKIK
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEEC
TGFGTGGWQLAALRWCAKAATKPIIADGGIRTHGDIAKSIRFGASMVMIGSLFAGHDESP
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCC
GETFEQDGKQLKEYFGSASEFQKGERKNVEGKKMFVEHKGALQDTLTEMEQDLQSAISYA
CHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHEEEEHHCCHHHHHHHHHHHHHHHHHHHC
GGDSLEALRTVDYVMVKNSIFNGDKVY
CCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA