BacMap

Definition	Exiguobacterium sp. AT1b, complete genome.
Accession	NC_012673
Length	2,999,895

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The map label for this gene is dkgB [H]

Identifier: 229917530

GI number: 229917530

Start: 1771397

End: 1772233

Strand: Reverse

Name: dkgB [H]

Synonym: EAT1b_1805

Alternate gene names: 229917530

Gene position: 1772233-1771397 (Counterclockwise)

Preceding gene: 229917531

Following gene: 229917529

Centisome position: 59.08

GC content: 51.14

Gene sequence:

>837_bases
ATGGGTATTCCAACAATTAAGCTACATGACGGTTATGAGATTCCGGCCATCGGACTCGGAACAGTTTATTTAAAAGGTGA
AGCAGGGGTCGACGCCATCACGTGCGCGATCCGGAACGGATATCGACTCATCGATTCGGCGATTCGCTATGACAATGAGG
GTGCGGTCGGTGAAGCGGTTCGTCAATCTGGCATCCCGCGCGAACAGCTTTTCTTAACGTCAAAATTGCGCGCGCAATAT
TATGACTACGACCAGGCACTCGAGATGATTCGGGAGTCCCTATATCGGGCGAACCTCGACTATTGGGATTTGTTCCTCTT
GCATTGGCCAAATCCGAAACAAGACAAGTACGTCGAGGCATGGCGTGCCTTGATTCAAGCGAAGAAAAACGGATGGATCC
GCTCCATTGGGGTATCGAACTTTATGCCGGAGCACCTTGACCGCTTGATTGAAGAAACGGGAGAAAAACCGGTCATCAAC
CAAATCGAGTTACACCCGTACTTCTCGCAAGTCGAACAGCGGCAAGCTGACAAAGAACGCGGCATCATCACAGAAGCGTG
GAGCCCGCTCAGTCGCGCCCGTACGGTTATTCATGACGAGAAGATTACCGAGCTCGCCAAACAAAAAGGCAAGAGCGTGT
CTCAAGTCATCTTACGTTGGCACGTGCAACTTGGGGTCATCCCGCTCGCCCGCTCGTCCAGTGAGTTGCACCAAAAAGAG
AATCTCGACGTCTTCAACTTCGAATTGACGGATGAAGAGATGGAGACGCTCAATGCGTTGACGAAGCCGGATGGTCGCAT
CGATGACCAAGATCCTCGTGAATACGAAGAGTATTAA

Upstream 100 bases:

>100_bases
GATCAAAGCGTTTCGACGTTGACCTCACATTCAAACGTTTCATTTCCGAGGAAAGTGGAATAGCGGAGAAGAGAACGGAT
TTTGAATGGGGAGGCATTTG

Downstream 100 bases:

>100_bases
TTAAATCGGGTTGAGCATGCGCTCAATCCGATTTTTGTATATATTCTAACTAAACTACTTAAAGGAGATTCTCATGCTAT
CCCAACAACGAAAAGACATG

Product: aldo/keto reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MGIPTIKLHDGYEIPAIGLGTVYLKGEAGVDAITCAIRNGYRLIDSAIRYDNEGAVGEAVRQSGIPREQLFLTSKLRAQY
YDYDQALEMIRESLYRANLDYWDLFLLHWPNPKQDKYVEAWRALIQAKKNGWIRSIGVSNFMPEHLDRLIEETGEKPVIN
QIELHPYFSQVEQRQADKERGIITEAWSPLSRARTVIHDEKITELAKQKGKSVSQVILRWHVQLGVIPLARSSSELHQKE
NLDVFNFELTDEEMETLNALTKPDGRIDDQDPREYEEY

Sequences:

>Translated_278_residues
MGIPTIKLHDGYEIPAIGLGTVYLKGEAGVDAITCAIRNGYRLIDSAIRYDNEGAVGEAVRQSGIPREQLFLTSKLRAQY
YDYDQALEMIRESLYRANLDYWDLFLLHWPNPKQDKYVEAWRALIQAKKNGWIRSIGVSNFMPEHLDRLIEETGEKPVIN
QIELHPYFSQVEQRQADKERGIITEAWSPLSRARTVIHDEKITELAKQKGKSVSQVILRWHVQLGVIPLARSSSELHQKE
NLDVFNFELTDEEMETLNALTKPDGRIDDQDPREYEEY
>Mature_277_residues
GIPTIKLHDGYEIPAIGLGTVYLKGEAGVDAITCAIRNGYRLIDSAIRYDNEGAVGEAVRQSGIPREQLFLTSKLRAQYY
DYDQALEMIRESLYRANLDYWDLFLLHWPNPKQDKYVEAWRALIQAKKNGWIRSIGVSNFMPEHLDRLIEETGEKPVINQ
IELHPYFSQVEQRQADKERGIITEAWSPLSRARTVIHDEKITELAKQKGKSVSQVILRWHVQLGVIPLARSSSELHQKEN
LDVFNFELTDEEMETLNALTKPDGRIDDQDPREYEEY

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=290, Percent_Identity=32.4137931034483, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI24497577, Length=296, Percent_Identity=33.1081081081081, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI5174391, Length=296, Percent_Identity=33.1081081081081, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI310109922, Length=278, Percent_Identity=34.1726618705036, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI5453543, Length=296, Percent_Identity=33.1081081081081, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI291291012, Length=281, Percent_Identity=32.3843416370107, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI310109920, Length=296, Percent_Identity=33.1081081081081, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI45446745, Length=296, Percent_Identity=32.7702702702703, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI4503285, Length=296, Percent_Identity=32.7702702702703, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI5174695, Length=300, Percent_Identity=34, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=32.8813559322034, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI24497583, Length=296, Percent_Identity=32.0945945945946, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI93277124, Length=296, Percent_Identity=30.4054054054054, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4502049, Length=283, Percent_Identity=30.7420494699647, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI300116273, Length=282, Percent_Identity=32.9787234042553, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI300116271, Length=284, Percent_Identity=31.6901408450704, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI310109924, Length=149, Percent_Identity=34.2281879194631, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI310109926, Length=127, Percent_Identity=36.2204724409449, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI207028673, Length=127, Percent_Identity=35.4330708661417, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI87082198, Length=277, Percent_Identity=41.5162454873646, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1786400, Length=253, Percent_Identity=35.9683794466403, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788081, Length=279, Percent_Identity=30.1075268817204, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71998625, Length=275, Percent_Identity=36.7272727272727, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17537077, Length=304, Percent_Identity=36.5131578947368, Blast_Score=170, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17537075, Length=304, Percent_Identity=35.1973684210526, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17561298, Length=273, Percent_Identity=34.7985347985348, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=31.6326530612245, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17564128, Length=297, Percent_Identity=30.6397306397306, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17538386, Length=283, Percent_Identity=32.8621908127208, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17566692, Length=289, Percent_Identity=30.7958477508651, Blast_Score=136, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17550248, Length=284, Percent_Identity=32.0422535211268, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17561300, Length=252, Percent_Identity=32.9365079365079, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17552492, Length=264, Percent_Identity=32.5757575757576, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17562292, Length=281, Percent_Identity=30.6049822064057, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324694, Length=290, Percent_Identity=34.8275862068965, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320576, Length=291, Percent_Identity=33.3333333333333, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322556, Length=261, Percent_Identity=33.3333333333333, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319625, Length=291, Percent_Identity=31.6151202749141, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321896, Length=314, Percent_Identity=28.6624203821656, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6320079, Length=282, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24663317, Length=286, Percent_Identity=36.013986013986, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI45553081, Length=288, Percent_Identity=35.0694444444444, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI20129731, Length=276, Percent_Identity=33.695652173913, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24657054, Length=285, Percent_Identity=36.140350877193, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI21356425, Length=287, Percent_Identity=33.7979094076655, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=30.952380952381, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24644950, Length=294, Percent_Identity=31.2925170068027, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI281366140, Length=252, Percent_Identity=33.7301587301587, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI221378297, Length=314, Percent_Identity=29.6178343949045, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24662781, Length=281, Percent_Identity=33.0960854092527, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24662785, Length=278, Percent_Identity=33.4532374100719, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 32102; Mature: 31970

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIPTIKLHDGYEIPAIGLGTVYLKGEAGVDAITCAIRNGYRLIDSAIRYDNEGAVGEAV
CCCCEEEECCCCCCCEEECEEEEEECCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHH
RQSGIPREQLFLTSKLRAQYYDYDQALEMIRESLYRANLDYWDLFLLHWPNPKQDKYVEA
HHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
WRALIQAKKNGWIRSIGVSNFMPEHLDRLIEETGEKPVINQIELHPYFSQVEQRQADKER
HHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHC
GIITEAWSPLSRARTVIHDEKITELAKQKGKSVSQVILRWHVQLGVIPLARSSSELHQKE
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEECCCCCHHHHHHC
NLDVFNFELTDEEMETLNALTKPDGRIDDQDPREYEEY
CCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
GIPTIKLHDGYEIPAIGLGTVYLKGEAGVDAITCAIRNGYRLIDSAIRYDNEGAVGEAV
CCCEEEECCCCCCCEEECEEEEEECCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHH
RQSGIPREQLFLTSKLRAQYYDYDQALEMIRESLYRANLDYWDLFLLHWPNPKQDKYVEA
HHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
WRALIQAKKNGWIRSIGVSNFMPEHLDRLIEETGEKPVINQIELHPYFSQVEQRQADKER
HHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHC
GIITEAWSPLSRARTVIHDEKITELAKQKGKSVSQVILRWHVQLGVIPLARSSSELHQKE
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEECCCCCHHHHHHC
NLDVFNFELTDEEMETLNALTKPDGRIDDQDPREYEEY
CCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA