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The map label for this gene is lip2 [H]

Identifier: 229917500

GI number: 229917500

Start: 1741029

End: 1742105

Strand: Direct

Name: lip2 [H]

Synonym: EAT1b_1775

Alternate gene names: 229917500

Gene position: 1741029-1742105 (Clockwise)

Preceding gene: 229917497

Following gene: 229917501

Centisome position: 58.04

GC content: 51.44

Gene sequence:

>1077_bases
ATGGAATCCGATTATAAATATCTCGTCCAACATCTTCAACAAAGCCAATATCGAACGCACGCGAACCACCTTGAAACCAT
CATCAAACCCATACCGGATTCAGACCGCACCGGCGATCTCGACTCACGCGTGTTCGAAACGCTGACCGCGAATCATTCTT
CACACGATGAACCGGATACAGCATTTGACCTCGAGGCGGCACGTGCCAGCATGGGTTGGCCGAATCAGGATCGGACGACG
ACTGACATCAAAACGGAGTATCTCATGATTCCTAGTGAGGACGGGGATATTCCGGTCCGTCTGTATCGTCCGAGTCATAC
TGAAGCCATCCCAGTCATCCTCTTCTTCCATGGTGGCGGGTTCTTCGGCGGAACGCTCGATACGGTCGAGAATCCTTGTA
AATTGCTTTCGGAACGGGCGAACGCGGTCGTCATCTCCGTCGATTATCGTCTTGCCCCGGAGCATCCGTTCCCGGCCGGA
TTGAATGACTGTTACCGCGCCTTTGAATGGGTACACGCACATTCGAATGAATTGAACATCCTCAAAGACCGAATCGGCGT
CGCCGGAGACAGTGCCGGAGGAAATTTGGCAACGGCTTGCTGTCTGATGGAGCAAGAACGAGGGGAGGGGCGCATTTGTT
ATCAAGCACTCGTCTATCCTGTCGTCAATCTGGGAACGATTCCGACAGATGATTTTGAATGGGCACTCGATGCGTACGAT
ATCTCACACCATCACGATCTGATTCGAGGTGTCGTGCTCGCACTCGCCGATGCGGACTCATTTTTTAATGACCTCTATCT
TCAAGGAATGGCGGATGTCACACATCCACTCGTATCACCATTATTCGCAGACGATTTCTCGAGTTTACCACCGACTTTGA
TTGTGACGGCCGAGTACGATTACTTACGACTTGAAGGAGAAGCGTATGCGCGGAAACTTTCAGGAGCGGGCGTTCCAACA
CGACTCGTTCAATATCGTGGGATGGACCATGCCTTCATGGACAAGTTGGGCGACTATCCACAGGCAGACGATTGCATGAA
TGAAATCGCAAAAGGACTGAAGGAGCTGACGAAATGA

Upstream 100 bases:

>100_bases
TCTGTTCCAACAACGTATCATGCGATGAGCCGATGTACAACTTTTCCCGATAACAATATTTTTATAGGTTCAGAAACATT
GAAAGAAAGGGGCTTACATA

Downstream 100 bases:

>100_bases
AACAGTATACATTGGATTGGACGCGTTATGAGGAATTGGCACGTCAAGCGGTCGCGGAAGGCGTCGTATTAGTTAAAAAC
GAACAGAAGACCCTCCCGTT

Product: alpha/beta hydrolase fold-3 domain protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MESDYKYLVQHLQQSQYRTHANHLETIIKPIPDSDRTGDLDSRVFETLTANHSSHDEPDTAFDLEAARASMGWPNQDRTT
TDIKTEYLMIPSEDGDIPVRLYRPSHTEAIPVILFFHGGGFFGGTLDTVENPCKLLSERANAVVISVDYRLAPEHPFPAG
LNDCYRAFEWVHAHSNELNILKDRIGVAGDSAGGNLATACCLMEQERGEGRICYQALVYPVVNLGTIPTDDFEWALDAYD
ISHHHDLIRGVVLALADADSFFNDLYLQGMADVTHPLVSPLFADDFSSLPPTLIVTAEYDYLRLEGEAYARKLSGAGVPT
RLVQYRGMDHAFMDKLGDYPQADDCMNEIAKGLKELTK

Sequences:

>Translated_358_residues
MESDYKYLVQHLQQSQYRTHANHLETIIKPIPDSDRTGDLDSRVFETLTANHSSHDEPDTAFDLEAARASMGWPNQDRTT
TDIKTEYLMIPSEDGDIPVRLYRPSHTEAIPVILFFHGGGFFGGTLDTVENPCKLLSERANAVVISVDYRLAPEHPFPAG
LNDCYRAFEWVHAHSNELNILKDRIGVAGDSAGGNLATACCLMEQERGEGRICYQALVYPVVNLGTIPTDDFEWALDAYD
ISHHHDLIRGVVLALADADSFFNDLYLQGMADVTHPLVSPLFADDFSSLPPTLIVTAEYDYLRLEGEAYARKLSGAGVPT
RLVQYRGMDHAFMDKLGDYPQADDCMNEIAKGLKELTK
>Mature_358_residues
MESDYKYLVQHLQQSQYRTHANHLETIIKPIPDSDRTGDLDSRVFETLTANHSSHDEPDTAFDLEAARASMGWPNQDRTT
TDIKTEYLMIPSEDGDIPVRLYRPSHTEAIPVILFFHGGGFFGGTLDTVENPCKLLSERANAVVISVDYRLAPEHPFPAG
LNDCYRAFEWVHAHSNELNILKDRIGVAGDSAGGNLATACCLMEQERGEGRICYQALVYPVVNLGTIPTDDFEWALDAYD
ISHHHDLIRGVVLALADADSFFNDLYLQGMADVTHPLVSPLFADDFSSLPPTLIVTAEYDYLRLEGEAYARKLSGAGVPT
RLVQYRGMDHAFMDKLGDYPQADDCMNEIAKGLKELTK

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=285, Percent_Identity=27.3684210526316, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI68299767, Length=299, Percent_Identity=26.4214046822742, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI206597554, Length=272, Percent_Identity=29.7794117647059, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI21328446, Length=91, Percent_Identity=43.956043956044, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI61966717, Length=316, Percent_Identity=26.2658227848101, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786682, Length=272, Percent_Identity=27.9411764705882, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI72001146, Length=315, Percent_Identity=27.6190476190476, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17567059, Length=289, Percent_Identity=30.7958477508651, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17540028, Length=175, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71996133, Length=141, Percent_Identity=31.2056737588652, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24656084, Length=99, Percent_Identity=38.3838383838384, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24656076, Length=99, Percent_Identity=38.3838383838384, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI20130169, Length=99, Percent_Identity=38.3838383838384, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 39928; Mature: 39928

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESDYKYLVQHLQQSQYRTHANHLETIIKPIPDSDRTGDLDSRVFETLTANHSSHDEPDT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
AFDLEAARASMGWPNQDRTTTDIKTEYLMIPSEDGDIPVRLYRPSHTEAIPVILFFHGGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEEEECCC
FFGGTLDTVENPCKLLSERANAVVISVDYRLAPEHPFPAGLNDCYRAFEWVHAHSNELNI
CCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHH
LKDRIGVAGDSAGGNLATACCLMEQERGEGRICYQALVYPVVNLGTIPTDDFEWALDAYD
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ISHHHDLIRGVVLALADADSFFNDLYLQGMADVTHPLVSPLFADDFSSLPPTLIVTAEYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
YLRLEGEAYARKLSGAGVPTRLVQYRGMDHAFMDKLGDYPQADDCMNEIAKGLKELTK
EEEECCHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MESDYKYLVQHLQQSQYRTHANHLETIIKPIPDSDRTGDLDSRVFETLTANHSSHDEPDT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
AFDLEAARASMGWPNQDRTTTDIKTEYLMIPSEDGDIPVRLYRPSHTEAIPVILFFHGGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEEEECCC
FFGGTLDTVENPCKLLSERANAVVISVDYRLAPEHPFPAGLNDCYRAFEWVHAHSNELNI
CCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHH
LKDRIGVAGDSAGGNLATACCLMEQERGEGRICYQALVYPVVNLGTIPTDDFEWALDAYD
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ISHHHDLIRGVVLALADADSFFNDLYLQGMADVTHPLVSPLFADDFSSLPPTLIVTAEYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
YLRLEGEAYARKLSGAGVPTRLVQYRGMDHAFMDKLGDYPQADDCMNEIAKGLKELTK
EEEECCHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]