Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 229917422

GI number: 229917422

Start: 1647536

End: 1649497

Strand: Reverse

Name: metG [H]

Synonym: EAT1b_1697

Alternate gene names: 229917422

Gene position: 1649497-1647536 (Counterclockwise)

Preceding gene: 229917424

Following gene: 229917421

Centisome position: 54.99

GC content: 48.27

Gene sequence:

>1962_bases
ATGGCGAAGCCGACTTTTTATATTACAACCCCAATTTATTATCCGAGTGCAAAACTGCATATCGGTCACGCATATACCAC
GGTAGCAGGTGATGCGATTGCGCGCTACAAACGCTTAAAAGGATTTGATGTCCGTTATTTGACCGGAACGGATGAGCACG
GTCAAAAGATTCAAGAAAAAGCGAAAGAAGCAGGCGTGACGCCACAAGCTTTCGTGGATGAAGTCGTGGAAGAGATCAAG
GTCTTATGGGACCGTCTCGAAATCTCGTACGATGACTACATCCGTACGACAGAGGACCGCCATAAGAAAGTGGTCGAGCA
TGTTTTCGAGACATTACTTAAAAATGGGGACATCTACCTCGGTGAGTATGAAGGATGGTATTCCGTACCGGATGAGACGT
ACTACACTGAATCGCAGCTCGTCGACGGAAAAAGTCCTGACAGTGGACATCCGGTCGAACTCGTTCGTGAGGAGTGCTAC
TTCTTCCGCCTCAGTAAATACGCGGACCGCTTGATTGAATATTTTGAGTCACATCCTGATTTTATTTTGCCAGAATCTCG
TAAAACCGAGATGATCAATAACTTCTTAAAGCCGGGTCTTCAAGATTTGGCTGTATCACGTACGACATTTGACTGGGGCG
TACAAGTCCCATCGAACCCGAAGCACGTCGTGTACGTATGGGTCGATGCGCTCACAAACTATATCTCATCACTCGGATAC
GGTACAGACAACGATGAACTCTTCAACCGTTACTGGCCAGCAGACGTTCATTTGGTAGGGAAAGAAATCGTCCGTTTCCA
TACGATCATTTGGCCAGCCCTCTTAATGGCGCTTGATCTTCCATTACCGAAGCAAGTATTTGCGCACGGATGGTTGCTCA
TGAAGGATGGGAAAATGTCGAAATCGAAAGGGAACGTCGTGGACCCAATCCCATTGATCGACCGTTACGGCCTCGACTCG
GTCCGCTATTATCTTCTCCGTGAAGTTCCATTCGGAGCGGATGGCACGTTCACGCCGGAAGCGTTCGTTGACCGCTTGAA
CTTTGATTTAGCGAACGACCTCGGAAACTTATTGAATCGCACCATTGCGATGATTAAAAAGTACTTCGATGGAAACATTC
CGGCATATAACGGAAATGTCACGGCATTTGATGCAGAATTGTTGAACGTGGTGAAAGAGACGACACGCAAAACAGAAGAA
GCCATGGAGCACATGGAGTTTTCGGTCGCGCTCACAAACATTTGGCAACTCGTCAGCCGTGCGAATAAATATATCGATGA
GACACAACCATGGGTTCTCGTGAAAGAGGAGTCGAAGCGCGAGGAGCTGGCTTCTGTCATGACACACCTTGCGGGCGTAC
TTCGTCATGTTGCCATCATGATTCAACCATTCATGACGCGCGCTCCAAAAGAGATGTTCCGTCAGCTCGGACTCGAGGGA
GAGGACATGTCATGGGAGGCGTTGGATACATTCGCAGACTTTAGTGACGCAGTTGTGACGGACGGGACACCGATTTTCCC
ACGTCGTGACGTGAAAGAAGAAGTCGATGCAATCCAAGAAATGATGCGTCAGGCGTCTAAAGCACGGATTGAAGAGACGG
AGACGGCATCAGTAGAAGAAGCTGACGAAGACATTCGTCCTGAAATCACGATTGATGTCTTCGATCAAGTCGAATTCCGT
GTCGGTCAAATCGTCGAAGCCGATAAGATTAAAAAAGCGAAGAAACTGTTGAAACTTCAAGTCGACCTTGGGAAAGAGAC
GCGTCAAATCGTGTCTGGTATCGCTGAATGGTATGCCCCTGAAGATTTGGTTGGCAAGAAAGTCATCGTTGTCGCAAACT
TGAAACCAGTCAAGCTCCGTGGAGAACTCTCGCAAGGGATGATTCTAGCTGCAGATCGTGACGGGAAACTAGAGCTCGCG
ACGGTCTCAGACCAGATGCCGAACGGTGCCATCGTCAAATAA

Upstream 100 bases:

>100_bases
AAGAAAACGCAAAAAAATTCCAAACAATTAGGTTTGGAAATTCTTTTGTCGAACCGTGTATAATGGAAATTGCATATGTC
GAAAGGATGGAGAATAAATC

Downstream 100 bases:

>100_bases
TGGAATGGGGGAGACCGCGGTCTCCCTCTCTTTTTCTAAGGAGGAAACAAGATGTTAATTGATACACATACGCACATCAA
TGCGGAACAGTTCAGTGAAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MAKPTFYITTPIYYPSAKLHIGHAYTTVAGDAIARYKRLKGFDVRYLTGTDEHGQKIQEKAKEAGVTPQAFVDEVVEEIK
VLWDRLEISYDDYIRTTEDRHKKVVEHVFETLLKNGDIYLGEYEGWYSVPDETYYTESQLVDGKSPDSGHPVELVREECY
FFRLSKYADRLIEYFESHPDFILPESRKTEMINNFLKPGLQDLAVSRTTFDWGVQVPSNPKHVVYVWVDALTNYISSLGY
GTDNDELFNRYWPADVHLVGKEIVRFHTIIWPALLMALDLPLPKQVFAHGWLLMKDGKMSKSKGNVVDPIPLIDRYGLDS
VRYYLLREVPFGADGTFTPEAFVDRLNFDLANDLGNLLNRTIAMIKKYFDGNIPAYNGNVTAFDAELLNVVKETTRKTEE
AMEHMEFSVALTNIWQLVSRANKYIDETQPWVLVKEESKREELASVMTHLAGVLRHVAIMIQPFMTRAPKEMFRQLGLEG
EDMSWEALDTFADFSDAVVTDGTPIFPRRDVKEEVDAIQEMMRQASKARIEETETASVEEADEDIRPEITIDVFDQVEFR
VGQIVEADKIKKAKKLLKLQVDLGKETRQIVSGIAEWYAPEDLVGKKVIVVANLKPVKLRGELSQGMILAADRDGKLELA
TVSDQMPNGAIVK

Sequences:

>Translated_653_residues
MAKPTFYITTPIYYPSAKLHIGHAYTTVAGDAIARYKRLKGFDVRYLTGTDEHGQKIQEKAKEAGVTPQAFVDEVVEEIK
VLWDRLEISYDDYIRTTEDRHKKVVEHVFETLLKNGDIYLGEYEGWYSVPDETYYTESQLVDGKSPDSGHPVELVREECY
FFRLSKYADRLIEYFESHPDFILPESRKTEMINNFLKPGLQDLAVSRTTFDWGVQVPSNPKHVVYVWVDALTNYISSLGY
GTDNDELFNRYWPADVHLVGKEIVRFHTIIWPALLMALDLPLPKQVFAHGWLLMKDGKMSKSKGNVVDPIPLIDRYGLDS
VRYYLLREVPFGADGTFTPEAFVDRLNFDLANDLGNLLNRTIAMIKKYFDGNIPAYNGNVTAFDAELLNVVKETTRKTEE
AMEHMEFSVALTNIWQLVSRANKYIDETQPWVLVKEESKREELASVMTHLAGVLRHVAIMIQPFMTRAPKEMFRQLGLEG
EDMSWEALDTFADFSDAVVTDGTPIFPRRDVKEEVDAIQEMMRQASKARIEETETASVEEADEDIRPEITIDVFDQVEFR
VGQIVEADKIKKAKKLLKLQVDLGKETRQIVSGIAEWYAPEDLVGKKVIVVANLKPVKLRGELSQGMILAADRDGKLELA
TVSDQMPNGAIVK
>Mature_652_residues
AKPTFYITTPIYYPSAKLHIGHAYTTVAGDAIARYKRLKGFDVRYLTGTDEHGQKIQEKAKEAGVTPQAFVDEVVEEIKV
LWDRLEISYDDYIRTTEDRHKKVVEHVFETLLKNGDIYLGEYEGWYSVPDETYYTESQLVDGKSPDSGHPVELVREECYF
FRLSKYADRLIEYFESHPDFILPESRKTEMINNFLKPGLQDLAVSRTTFDWGVQVPSNPKHVVYVWVDALTNYISSLGYG
TDNDELFNRYWPADVHLVGKEIVRFHTIIWPALLMALDLPLPKQVFAHGWLLMKDGKMSKSKGNVVDPIPLIDRYGLDSV
RYYLLREVPFGADGTFTPEAFVDRLNFDLANDLGNLLNRTIAMIKKYFDGNIPAYNGNVTAFDAELLNVVKETTRKTEEA
MEHMEFSVALTNIWQLVSRANKYIDETQPWVLVKEESKREELASVMTHLAGVLRHVAIMIQPFMTRAPKEMFRQLGLEGE
DMSWEALDTFADFSDAVVTDGTPIFPRRDVKEEVDAIQEMMRQASKARIEETETASVEEADEDIRPEITIDVFDQVEFRV
GQIVEADKIKKAKKLLKLQVDLGKETRQIVSGIAEWYAPEDLVGKKVIVVANLKPVKLRGELSQGMILAADRDGKLELAT
VSDQMPNGAIVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=506, Percent_Identity=34.7826086956522, Blast_Score=305, Evalue=1e-82,
Organism=Homo sapiens, GI14043022, Length=654, Percent_Identity=27.5229357798165, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI7661872, Length=183, Percent_Identity=27.8688524590164, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1788432, Length=673, Percent_Identity=28.9747399702823, Blast_Score=287, Evalue=2e-78,
Organism=Escherichia coli, GI1789455, Length=101, Percent_Identity=42.5742574257426, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786861, Length=173, Percent_Identity=26.0115606936416, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=491, Percent_Identity=32.9938900203666, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71992781, Length=377, Percent_Identity=30.7692307692308, Blast_Score=193, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17540794, Length=563, Percent_Identity=25.3996447602131, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71997510, Length=170, Percent_Identity=30.5882352941176, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71997517, Length=170, Percent_Identity=30.5882352941176, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321610, Length=513, Percent_Identity=33.7231968810916, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6321704, Length=518, Percent_Identity=25.6756756756757, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323414, Length=215, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358483, Length=509, Percent_Identity=35.5599214145383, Blast_Score=299, Evalue=5e-81,
Organism=Drosophila melanogaster, GI24655311, Length=524, Percent_Identity=26.3358778625954, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21355409, Length=176, Percent_Identity=29.5454545454545, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 74509; Mature: 74378

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPTFYITTPIYYPSAKLHIGHAYTTVAGDAIARYKRLKGFDVRYLTGTDEHGQKIQEK
CCCCCEEEECCEEECCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
AKEAGVTPQAFVDEVVEEIKVLWDRLEISYDDYIRTTEDRHKKVVEHVFETLLKNGDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
GEYEGWYSVPDETYYTESQLVDGKSPDSGHPVELVREECYFFRLSKYADRLIEYFESHPD
ECCCCCCCCCCCCEECHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FILPESRKTEMINNFLKPGLQDLAVSRTTFDWGVQVPSNPKHVVYVWVDALTNYISSLGY
EECCCCHHHHHHHHHHCCCHHHHHHHCCEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCC
GTDNDELFNRYWPADVHLVGKEIVRFHTIIWPALLMALDLPLPKQVFAHGWLLMKDGKMS
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCC
KSKGNVVDPIPLIDRYGLDSVRYYLLREVPFGADGTFTPEAFVDRLNFDLANDLGNLLNR
CCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
TIAMIKKYFDGNIPAYNGNVTAFDAELLNVVKETTRKTEEAMEHMEFSVALTNIWQLVSR
HHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANKYIDETQPWVLVKEESKREELASVMTHLAGVLRHVAIMIQPFMTRAPKEMFRQLGLEG
HHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
EDMSWEALDTFADFSDAVVTDGTPIFPRRDVKEEVDAIQEMMRQASKARIEETETASVEE
CCCCHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ADEDIRPEITIDVFDQVEFRVGQIVEADKIKKAKKLLKLQVDLGKETRQIVSGIAEWYAP
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
EDLVGKKVIVVANLKPVKLRGELSQGMILAADRDGKLELATVSDQMPNGAIVK
HHHCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AKPTFYITTPIYYPSAKLHIGHAYTTVAGDAIARYKRLKGFDVRYLTGTDEHGQKIQEK
CCCCEEEECCEEECCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
AKEAGVTPQAFVDEVVEEIKVLWDRLEISYDDYIRTTEDRHKKVVEHVFETLLKNGDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
GEYEGWYSVPDETYYTESQLVDGKSPDSGHPVELVREECYFFRLSKYADRLIEYFESHPD
ECCCCCCCCCCCCEECHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FILPESRKTEMINNFLKPGLQDLAVSRTTFDWGVQVPSNPKHVVYVWVDALTNYISSLGY
EECCCCHHHHHHHHHHCCCHHHHHHHCCEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCC
GTDNDELFNRYWPADVHLVGKEIVRFHTIIWPALLMALDLPLPKQVFAHGWLLMKDGKMS
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCC
KSKGNVVDPIPLIDRYGLDSVRYYLLREVPFGADGTFTPEAFVDRLNFDLANDLGNLLNR
CCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
TIAMIKKYFDGNIPAYNGNVTAFDAELLNVVKETTRKTEEAMEHMEFSVALTNIWQLVSR
HHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANKYIDETQPWVLVKEESKREELASVMTHLAGVLRHVAIMIQPFMTRAPKEMFRQLGLEG
HHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
EDMSWEALDTFADFSDAVVTDGTPIFPRRDVKEEVDAIQEMMRQASKARIEETETASVEE
CCCCHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ADEDIRPEITIDVFDQVEFRVGQIVEADKIKKAKKLLKLQVDLGKETRQIVSGIAEWYAP
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
EDLVGKKVIVVANLKPVKLRGELSQGMILAADRDGKLELATVSDQMPNGAIVK
HHHCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]