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The map label for this gene is cysS

Identifier: 229917379

GI number: 229917379

Start: 1595955

End: 1597352

Strand: Reverse

Name: cysS

Synonym: EAT1b_1654

Alternate gene names: 229917379

Gene position: 1597352-1595955 (Counterclockwise)

Preceding gene: 229917380

Following gene: 229917378

Centisome position: 53.25

GC content: 49.71

Gene sequence:

>1398_bases
ATGATTCAGCTGTACAACAGTCTCACGAATAAAAAAGAACCGTTTGTCCCGATTGTTCCGGGTAAAGTATCGATGTACGT
ATGCGGTCCGACCGTTTATAACTACATTCATGTCGGAAACGCCCGACCGGCGATCGCCTTCGACACGGTCCGCCGCTACT
TGACGTATCGTGGGTACGAGGTCAAATACGTCTTGAACTTTACAGACGTGGATGACAAGATCATTCGTACGGCGAATGAA
TTAGGAGAAGACACGGAGACGCTGACGAATCGCTATATCGAGGCGTATTTAGCAGATACAGGGGCACTTAACGTTCAACC
GGCAGACGTGCATCCGCGTGTTACTGATACGATGGAAGAAATCATCGAGTTCATTCGTCAATTGGAGACGGAAGGGTTCG
CTTATGAATCAGAAGGAGACGTGTATTTCCGGACGAAGAAGTTTGAGACGTACGGGAAGCTGAGTCAGCAATCGATTGAA
GATTTGCGGGCCGGTTCACGTGTCGATGTCGGAGAGAAGAAAGAAGACCCGCTCGACTTCGTTCTATGGAAAGCGGCTAA
ACCGGATGAACCATCATGGGATAGTCCGTGGGGCAAAGGACGTCCGGGCTGGCATATCGAATGCTCGGCGATGGCGAAAA
AACATCTCGGGACAACGATTGATATTCACGCGGGCGGGCATGATTTAAAATTCCCACACCACGAGAACGAGATTGCTCAG
TCGGAAGCTTGCAACCATGCGAAGTTCGCCAACTATTGGCTGCATAACGGCTTCATTAACATTGAAAATGAGAAGATGTC
TAAGTCGCTCGGAAACTTCCTCCTCGTCCATGAGGCGTTGAAAGAAGTCGACCCGATGGTCCTGCGCTTCTTCATGTTGT
CTGTCCATTATCGTCATCCGATCAACTATAGCCGAGAATTGATTGAACAAGCGGCGAACGGCTGGAGCCGAATCAAGGAA
GCTTACCAAAATATTGAATATCGCTTGTCGGTCACAGCTGGACTTGGAGAGGCGAGCGAGTCGATGGAACGGAAGCTTGA
ATCCATCAAGGCGTCTTTCATCGAGTCAATGGATGATGATATCAATACGGCCAACGCCGTGACCGTCCTCTTTGACTTGG
CACGGGAAGCGAACATTTATGCGAAGGCCGATCATGTGGCAAAAGCGACGCTTGAACAGGTCCTGTCGCTATTTGATGAA
TTGACGGGTGTCCTCGGCTTGACGTTGGCAGAAGAAAAAGAACTATTGGATGCCGAGATCGACCAATTGATTCAAGAACG
GAACGACGCACGGGCGGCACGTAATTTTGCCCGGGCCGACGAGATTCGTGATTTACTAAAAGAACAAAACATCCAACTCG
AAGACACGGCCCAAGGGGTTCGGTGGAAGCGGTTATGA

Upstream 100 bases:

>100_bases
CCAGATCCGGACCGCGAATGCCAAGAACGCTTAGAAGCGGCAGTGGATGAACTACAACAAAAATTAGCGACGCTTGAAAA
GCGGATGGAGGAGAAGACAC

Downstream 100 bases:

>100_bases
TGCAACCGAAACAAATGAATGCCCTCGCTCTCGCTTATATGGGAGACGTGGTATACGAAATGGCTGTACGGAGACGCCTG
CTTGAGAAAGGATTGACGCG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MIQLYNSLTNKKEPFVPIVPGKVSMYVCGPTVYNYIHVGNARPAIAFDTVRRYLTYRGYEVKYVLNFTDVDDKIIRTANE
LGEDTETLTNRYIEAYLADTGALNVQPADVHPRVTDTMEEIIEFIRQLETEGFAYESEGDVYFRTKKFETYGKLSQQSIE
DLRAGSRVDVGEKKEDPLDFVLWKAAKPDEPSWDSPWGKGRPGWHIECSAMAKKHLGTTIDIHAGGHDLKFPHHENEIAQ
SEACNHAKFANYWLHNGFINIENEKMSKSLGNFLLVHEALKEVDPMVLRFFMLSVHYRHPINYSRELIEQAANGWSRIKE
AYQNIEYRLSVTAGLGEASESMERKLESIKASFIESMDDDINTANAVTVLFDLAREANIYAKADHVAKATLEQVLSLFDE
LTGVLGLTLAEEKELLDAEIDQLIQERNDARAARNFARADEIRDLLKEQNIQLEDTAQGVRWKRL

Sequences:

>Translated_465_residues
MIQLYNSLTNKKEPFVPIVPGKVSMYVCGPTVYNYIHVGNARPAIAFDTVRRYLTYRGYEVKYVLNFTDVDDKIIRTANE
LGEDTETLTNRYIEAYLADTGALNVQPADVHPRVTDTMEEIIEFIRQLETEGFAYESEGDVYFRTKKFETYGKLSQQSIE
DLRAGSRVDVGEKKEDPLDFVLWKAAKPDEPSWDSPWGKGRPGWHIECSAMAKKHLGTTIDIHAGGHDLKFPHHENEIAQ
SEACNHAKFANYWLHNGFINIENEKMSKSLGNFLLVHEALKEVDPMVLRFFMLSVHYRHPINYSRELIEQAANGWSRIKE
AYQNIEYRLSVTAGLGEASESMERKLESIKASFIESMDDDINTANAVTVLFDLAREANIYAKADHVAKATLEQVLSLFDE
LTGVLGLTLAEEKELLDAEIDQLIQERNDARAARNFARADEIRDLLKEQNIQLEDTAQGVRWKRL
>Mature_465_residues
MIQLYNSLTNKKEPFVPIVPGKVSMYVCGPTVYNYIHVGNARPAIAFDTVRRYLTYRGYEVKYVLNFTDVDDKIIRTANE
LGEDTETLTNRYIEAYLADTGALNVQPADVHPRVTDTMEEIIEFIRQLETEGFAYESEGDVYFRTKKFETYGKLSQQSIE
DLRAGSRVDVGEKKEDPLDFVLWKAAKPDEPSWDSPWGKGRPGWHIECSAMAKKHLGTTIDIHAGGHDLKFPHHENEIAQ
SEACNHAKFANYWLHNGFINIENEKMSKSLGNFLLVHEALKEVDPMVLRFFMLSVHYRHPINYSRELIEQAANGWSRIKE
AYQNIEYRLSVTAGLGEASESMERKLESIKASFIESMDDDINTANAVTVLFDLAREANIYAKADHVAKATLEQVLSLFDE
LTGVLGLTLAEEKELLDAEIDQLIQERNDARAARNFARADEIRDLLKEQNIQLEDTAQGVRWKRL

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=477, Percent_Identity=37.1069182389937, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI303304987, Length=318, Percent_Identity=37.1069182389937, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI21269877, Length=318, Percent_Identity=37.1069182389937, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI10835051, Length=318, Percent_Identity=37.1069182389937, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI62240992, Length=318, Percent_Identity=37.1069182389937, Blast_Score=212, Evalue=6e-55,
Organism=Escherichia coli, GI1786737, Length=475, Percent_Identity=47.3684210526316, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17509795, Length=338, Percent_Identity=35.207100591716, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17509797, Length=338, Percent_Identity=35.207100591716, Blast_Score=194, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6324082, Length=329, Percent_Identity=36.7781155015198, Blast_Score=209, Evalue=8e-55,
Organism=Drosophila melanogaster, GI19922218, Length=510, Percent_Identity=34.7058823529412, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI19922364, Length=347, Percent_Identity=34.8703170028818, Blast_Score=201, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_EXISA (C4KZR7)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886025.1
- GeneID:   7869715
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1654
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53076; Mature: 53076

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQLYNSLTNKKEPFVPIVPGKVSMYVCGPTVYNYIHVGNARPAIAFDTVRRYLTYRGYE
CEEHHHHHCCCCCCCEEECCCCEEEEEECHHHHHEEEECCCCCCHHHHHHHHHHHHCCEE
VKYVLNFTDVDDKIIRTANELGEDTETLTNRYIEAYLADTGALNVQPADVHPRVTDTMEE
EEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
IIEFIRQLETEGFAYESEGDVYFRTKKFETYGKLSQQSIEDLRAGSRVDVGEKKEDPLDF
HHHHHHHHHHCCCEECCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHE
VLWKAAKPDEPSWDSPWGKGRPGWHIECSAMAKKHLGTTIDIHAGGHDLKFPHHENEIAQ
EEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHH
SEACNHAKFANYWLHNGFINIENEKMSKSLGNFLLVHEALKEVDPMVLRFFMLSVHYRHP
HHCCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
INYSRELIEQAANGWSRIKEAYQNIEYRLSVTAGLGEASESMERKLESIKASFIESMDDD
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
INTANAVTVLFDLAREANIYAKADHVAKATLEQVLSLFDELTGVLGLTLAEEKELLDAEI
CCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQLIQERNDARAARNFARADEIRDLLKEQNIQLEDTAQGVRWKRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCC
>Mature Secondary Structure
MIQLYNSLTNKKEPFVPIVPGKVSMYVCGPTVYNYIHVGNARPAIAFDTVRRYLTYRGYE
CEEHHHHHCCCCCCCEEECCCCEEEEEECHHHHHEEEECCCCCCHHHHHHHHHHHHCCEE
VKYVLNFTDVDDKIIRTANELGEDTETLTNRYIEAYLADTGALNVQPADVHPRVTDTMEE
EEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
IIEFIRQLETEGFAYESEGDVYFRTKKFETYGKLSQQSIEDLRAGSRVDVGEKKEDPLDF
HHHHHHHHHHCCCEECCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHE
VLWKAAKPDEPSWDSPWGKGRPGWHIECSAMAKKHLGTTIDIHAGGHDLKFPHHENEIAQ
EEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHH
SEACNHAKFANYWLHNGFINIENEKMSKSLGNFLLVHEALKEVDPMVLRFFMLSVHYRHP
HHCCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
INYSRELIEQAANGWSRIKEAYQNIEYRLSVTAGLGEASESMERKLESIKASFIESMDDD
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
INTANAVTVLFDLAREANIYAKADHVAKATLEQVLSLFDELTGVLGLTLAEEKELLDAEI
CCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQLIQERNDARAARNFARADEIRDLLKEQNIQLEDTAQGVRWKRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA