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The map label for this gene is tuf [H]

Identifier: 229917361

GI number: 229917361

Start: 1576952

End: 1578139

Strand: Reverse

Name: tuf [H]

Synonym: EAT1b_1636

Alternate gene names: 229917361

Gene position: 1578139-1576952 (Counterclockwise)

Preceding gene: 229917362

Following gene: 229917360

Centisome position: 52.61

GC content: 47.47

Gene sequence:

>1188_bases
ATGGGTAAAGAAAAATTCGACCGTTCTAAACCGCACGTTAACGTTGGTACAATCGGCCACGTCGACCACGGTAAAACAAC
TTTGACTGCAGCAATCTCTGCTGTACTTTCAAAAGCACAAGGTAAAGCAGCTACTAAGTTCGACCAAATCGATGGTGCTC
CAGAAGAGCGCGAGCGCGGTATCACAATCGCAACAGCTCACATCGAGTACGAAACTGACAAGCGTCACTACGCACACGTT
GACTGCCCAGGACACGCTGACTATGTTAAAAACATGATCACTGGTGCTGCACAAATGGATGGCGCGATCCTCGTTGTATC
TGCAACAGATGGCCCAATGCCACAAACACGTGAGCACATCCTTCTTTCACGTCAAGTAGGTGTTCCTTACATCGTAGTAT
TCATGAACAAAGTTGACATGGTAGACGACGAAGAGCTTCTCGAACTCGTTGAAATGGAAATCCGTGAACTTCTTTCTGAA
TACGACTTCCCAGGCGACGACCTCCCTGTAATCAAAGGTTCTGCACTTGGCGCACTTAACGGTGAAGCTCAGTGGGAAGA
AAAAGTTATGGAACTCATGAACGCTGTTGATGAGTACATCCCAGAACCAGTTCGTGACACTGAAAAAGACTTCATGATGC
CTGTTGAGGACGTATTCTCAATCACTGGTCGTGGTACTGTAGCGACTGGTCGTGTTGAGCGTGGTGTTCTCCGCGTCAAC
GACGAGATCGAAATCGTTGGTCTTACAGAAGAAACGAAGAAAACTGTATGTACTGGTGTAGAGATGTTCCGTAAGCTTCT
TGACTATGCTGAAGCTGGCGACAACATCGGTGCACTTCTCCGTGGTGTATCACGTGACGACATCGAGCGTGGACAAGTAC
TTGCTAAGCCAGGTTCAATCACACCACACTCTAAATTCAAAGCATCAATCTACGTTCTTTCTAAAGAAGAGGGTGGCCGT
CACACGCCATTCTTCGCGAACTACCGCCCGCAGTTCTACTTCCGTACAACTGACGTAACAGGTATCGTTCACCTTCCAGA
AGGTACTGAAATGGTAATGCCAGGAGACAACATCGAGTTGACGATCGAATTGATTTCTACAATCGCGATCGAAGACGGTA
CACGTTTCTCTATCCGTGAGGGTGGCCGTACTGTAGGTGCTGGTTCAGTAACAGAAATCATCGAGTAA

Upstream 100 bases:

>100_bases
GCTAGATTGATTGTTGTAAGCTAAGGAGGACGTATGTTAAAAGTAGCCTACGTCTTCCTAGTTAAATAAAACTAATCGTT
TAATAGAGGAGGAATCTAGA

Downstream 100 bases:

>100_bases
TCGATTGGTTCAACTGAACAACATGAAGCAGTCCGCAATATGCGGGCTGCTTTTTTGTGTTTTCATCACAACATGTTAAG
ATGAGGCATACGAGAAAGGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MGKEKFDRSKPHVNVGTIGHVDHGKTTLTAAISAVLSKAQGKAATKFDQIDGAPEERERGITIATAHIEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHILLSRQVGVPYIVVFMNKVDMVDDEELLELVEMEIRELLSE
YDFPGDDLPVIKGSALGALNGEAQWEEKVMELMNAVDEYIPEPVRDTEKDFMMPVEDVFSITGRGTVATGRVERGVLRVN
DEIEIVGLTEETKKTVCTGVEMFRKLLDYAEAGDNIGALLRGVSRDDIERGQVLAKPGSITPHSKFKASIYVLSKEEGGR
HTPFFANYRPQFYFRTTDVTGIVHLPEGTEMVMPGDNIELTIELISTIAIEDGTRFSIREGGRTVGAGSVTEIIE

Sequences:

>Translated_395_residues
MGKEKFDRSKPHVNVGTIGHVDHGKTTLTAAISAVLSKAQGKAATKFDQIDGAPEERERGITIATAHIEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHILLSRQVGVPYIVVFMNKVDMVDDEELLELVEMEIRELLSE
YDFPGDDLPVIKGSALGALNGEAQWEEKVMELMNAVDEYIPEPVRDTEKDFMMPVEDVFSITGRGTVATGRVERGVLRVN
DEIEIVGLTEETKKTVCTGVEMFRKLLDYAEAGDNIGALLRGVSRDDIERGQVLAKPGSITPHSKFKASIYVLSKEEGGR
HTPFFANYRPQFYFRTTDVTGIVHLPEGTEMVMPGDNIELTIELISTIAIEDGTRFSIREGGRTVGAGSVTEIIE
>Mature_394_residues
GKEKFDRSKPHVNVGTIGHVDHGKTTLTAAISAVLSKAQGKAATKFDQIDGAPEERERGITIATAHIEYETDKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHILLSRQVGVPYIVVFMNKVDMVDDEELLELVEMEIRELLSEY
DFPGDDLPVIKGSALGALNGEAQWEEKVMELMNAVDEYIPEPVRDTEKDFMMPVEDVFSITGRGTVATGRVERGVLRVND
EIEIVGLTEETKKTVCTGVEMFRKLLDYAEAGDNIGALLRGVSRDDIERGQVLAKPGSITPHSKFKASIYVLSKEEGGRH
TPFFANYRPQFYFRTTDVTGIVHLPEGTEMVMPGDNIELTIELISTIAIEDGTRFSIREGGRTVGAGSVTEIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=55.9493670886076, Blast_Score=465, Evalue=1e-131,
Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=30.1801801801802, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=30.1801801801802, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503475, Length=363, Percent_Identity=31.9559228650138, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4503471, Length=363, Percent_Identity=32.5068870523416, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=27.7258566978193, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194018522, Length=344, Percent_Identity=27.6162790697674, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194018520, Length=344, Percent_Identity=27.6162790697674, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI194097354, Length=344, Percent_Identity=27.6162790697674, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI46094014, Length=336, Percent_Identity=27.9761904761905, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4503507, Length=362, Percent_Identity=27.6243093922652, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI157426893, Length=261, Percent_Identity=29.8850574712644, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=34.0909090909091, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=73.0964467005076, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=73.0964467005076, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI2367247, Length=383, Percent_Identity=28.9817232375979, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI48994988, Length=269, Percent_Identity=30.4832713754647, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789108, Length=253, Percent_Identity=33.596837944664, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=29.4520547945205, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=54.726368159204, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=44.300518134715, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=340, Percent_Identity=34.4117647058824, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=340, Percent_Identity=34.4117647058824, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=322, Percent_Identity=33.2298136645963, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=27.0642201834862, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=27.0642201834862, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=342, Percent_Identity=28.3625730994152, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=357, Percent_Identity=27.7310924369748, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=40.9090909090909, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=368, Percent_Identity=27.1739130434783, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=26.8398268398268, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=64.8989898989899, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=363, Percent_Identity=33.0578512396694, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=363, Percent_Identity=33.0578512396694, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=25.3897550111359, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=283, Percent_Identity=28.6219081272085, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=352, Percent_Identity=27.8409090909091, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=29.9180327868852, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=200, Percent_Identity=31, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=56.3451776649746, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=56.3451776649746, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=47.9381443298969, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45553807, Length=363, Percent_Identity=33.0578512396694, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45553816, Length=363, Percent_Identity=33.0578512396694, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24651721, Length=363, Percent_Identity=33.0578512396694, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI17864154, Length=363, Percent_Identity=33.0578512396694, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24652838, Length=361, Percent_Identity=32.9639889196676, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=361, Percent_Identity=32.9639889196676, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=32.2368421052632, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45550900, Length=444, Percent_Identity=29.0540540540541, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=29.1545189504373, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647048, Length=369, Percent_Identity=27.9132791327913, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24647046, Length=369, Percent_Identity=27.9132791327913, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28572034, Length=170, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706572, Length=238, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43483; Mature: 43352

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEKFDRSKPHVNVGTIGHVDHGKTTLTAAISAVLSKAQGKAATKFDQIDGAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHCC
ITIATAHIEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPYIVVFMNKVDMVDDEELLELVEMEIRELLSEYDFPGDDLPVIKGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEAQWEEKVMELMNAVDEYIPEPVRDTEKDFMMPVEDVFSITGRGTVATGRVERGVLRVN
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCCCCEEEECCHHCCEEEEC
DEIEIVGLTEETKKTVCTGVEMFRKLLDYAEAGDNIGALLRGVSRDDIERGQVLAKPGSI
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCCCEEEECCCCC
TPHSKFKASIYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGIVHLPEGTEMVMPGDNIEL
CCCCCCEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCCEE
TIELISTIAIEDGTRFSIREGGRTVGAGSVTEIIE
EEEEEEHHEECCCCEEEECCCCCEECCCCHHHHCC
>Mature Secondary Structure 
GKEKFDRSKPHVNVGTIGHVDHGKTTLTAAISAVLSKAQGKAATKFDQIDGAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHCC
ITIATAHIEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPYIVVFMNKVDMVDDEELLELVEMEIRELLSEYDFPGDDLPVIKGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEAQWEEKVMELMNAVDEYIPEPVRDTEKDFMMPVEDVFSITGRGTVATGRVERGVLRVN
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCCCCEEEECCHHCCEEEEC
DEIEIVGLTEETKKTVCTGVEMFRKLLDYAEAGDNIGALLRGVSRDDIERGQVLAKPGSI
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCCCEEEECCCCC
TPHSKFKASIYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGIVHLPEGTEMVMPGDNIEL
CCCCCCEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCCEE
TIELISTIAIEDGTRFSIREGGRTVGAGSVTEIIE
EEEEEEHHEECCCCEEEECCCCCEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA