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The map label for this gene is rfbA [H]

Identifier: 229917238

GI number: 229917238

Start: 1481494

End: 1482831

Strand: Direct

Name: rfbA [H]

Synonym: EAT1b_1513

Alternate gene names: 229917238

Gene position: 1481494-1482831 (Clockwise)

Preceding gene: 229917236

Following gene: 229917247

Centisome position: 49.38

GC content: 48.13

Gene sequence:

>1338_bases
ATGAAACTACTTCTATTATCCGGTGGGTCTGGAAAACGTCTCTGGCCTCTTTCAAATGATATCAACTCGAAACAGTTCAT
TCGATTGCTCGACGACGGACACGGTTCTAAACAATCCATGATTGAACGCGTCTTTTCACAACTGAGTGACATGAAGTTGA
ATCAGGACGCCTATATTCTGACGAATGCACATCAGCGTGATGCCATCGTCTATCAAATCGGGACCGACGCCAATATCATC
ACCGAACCAAGTCGCCGCGACACATTCCCCGCAATCGTTCTTGGCGCAAGTTATTTGAACGAACAACAGGTCGACCCGGA
TGAAACGGTACTTGTGATGCCGGTCGATGCTTATGTCGACCTCCGCTTCTTTGAATATTTCCCTCAAGTCGATGCGGCCG
TCCAAGCGAGCGACGATGCCTTGCATTTGATTGGGGTCACCCCTCTTCATCCGACATCAAAATACGGATATATCGTTCCA
GACGAGACGGCTCAAGTATCCTCGGTTCGCCTGTTCCAGGAAAAACCGACCGTCGACGTGGCGGAACAGTTGATTGGACA
AGGTGCGCTTTGGAACTGTGGAATCTTTGCTTGCAAGTTGCATCTGTTACTTGATGAACTGTCAGAACGAAACTTACCGA
CAACTTATTCAGACCTCACTTCACGCTATGACGAATTAGAAAAGACTTCGTTCGACTATGCTGTTGTCGAACATGCGGAG
TCCGTATTCGTTCACCGCTACAACGGCGATTGGAAAGACTTAGGTACATGGAACACGTTGACTGAAGAAATGACGTCCAC
AACTCACGGTCAAGTCGACCTATTTGAATCGACGAACACCCATGTGATCAATGAATTGAATCTTCCGTTGATTGGGATCG
GATTAGAAGACCTTGTCGTCGCCGTCAACGCGGATGGCATTCTCGTTTCAACGAAAGAGGCGACCCCACAGCTGAAAGAT
TATCTCGCCTCGTCCGACGACTATCCAAAATTCAAATGGAAGCGATGGGGGCAGTCGGAAGTCATCCATCATGAGACACT
CGCAGACGGACATCACAGTGTGACACGACGCATGCTCGTTCAAGCCGGAAAAGAACTCAGTTATCATTATCATGAAGAGA
CCGATACGATTTGGACGATGCTTCAAGGATCAGCAACCGTGACGATTGATGGAGAAACGTTTGAGGCGAAGCCGACCGAC
GTCATCTCGATTCAACGTGGTGCGGCTCACACGCTCAAAGCAAAAGAAGATGTCGTGTTTATCGAGGTTCAGTATTCAAA
CGACTGGACACTTGACGATGCTTGTCCTGTGCCTTCTCCACTTGATAAACAGTATTGA

Upstream 100 bases:

>100_bases
CAGACGAGCCAACGTCTTGCGCGTGTGGTCATGCGGTTCGACGAGTAAGTAATTTGCCATATTGTGTACCTCCATTTTCA
TAAAGAAGGGATGTTACGTA

Downstream 100 bases:

>100_bases
TGCGTTGTGTCGTTGGTACAGGGACTTCACTTGTGACGATTTCCACCTTTCCGGTGAACAAGTGAGGTCCTTTTCCTTTT
GGAATGAAGTAATGTTCCCC

Product: Mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKLLLLSGGSGKRLWPLSNDINSKQFIRLLDDGHGSKQSMIERVFSQLSDMKLNQDAYILTNAHQRDAIVYQIGTDANII
TEPSRRDTFPAIVLGASYLNEQQVDPDETVLVMPVDAYVDLRFFEYFPQVDAAVQASDDALHLIGVTPLHPTSKYGYIVP
DETAQVSSVRLFQEKPTVDVAEQLIGQGALWNCGIFACKLHLLLDELSERNLPTTYSDLTSRYDELEKTSFDYAVVEHAE
SVFVHRYNGDWKDLGTWNTLTEEMTSTTHGQVDLFESTNTHVINELNLPLIGIGLEDLVVAVNADGILVSTKEATPQLKD
YLASSDDYPKFKWKRWGQSEVIHHETLADGHHSVTRRMLVQAGKELSYHYHEETDTIWTMLQGSATVTIDGETFEAKPTD
VISIQRGAAHTLKAKEDVVFIEVQYSNDWTLDDACPVPSPLDKQY

Sequences:

>Translated_445_residues
MKLLLLSGGSGKRLWPLSNDINSKQFIRLLDDGHGSKQSMIERVFSQLSDMKLNQDAYILTNAHQRDAIVYQIGTDANII
TEPSRRDTFPAIVLGASYLNEQQVDPDETVLVMPVDAYVDLRFFEYFPQVDAAVQASDDALHLIGVTPLHPTSKYGYIVP
DETAQVSSVRLFQEKPTVDVAEQLIGQGALWNCGIFACKLHLLLDELSERNLPTTYSDLTSRYDELEKTSFDYAVVEHAE
SVFVHRYNGDWKDLGTWNTLTEEMTSTTHGQVDLFESTNTHVINELNLPLIGIGLEDLVVAVNADGILVSTKEATPQLKD
YLASSDDYPKFKWKRWGQSEVIHHETLADGHHSVTRRMLVQAGKELSYHYHEETDTIWTMLQGSATVTIDGETFEAKPTD
VISIQRGAAHTLKAKEDVVFIEVQYSNDWTLDDACPVPSPLDKQY
>Mature_445_residues
MKLLLLSGGSGKRLWPLSNDINSKQFIRLLDDGHGSKQSMIERVFSQLSDMKLNQDAYILTNAHQRDAIVYQIGTDANII
TEPSRRDTFPAIVLGASYLNEQQVDPDETVLVMPVDAYVDLRFFEYFPQVDAAVQASDDALHLIGVTPLHPTSKYGYIVP
DETAQVSSVRLFQEKPTVDVAEQLIGQGALWNCGIFACKLHLLLDELSERNLPTTYSDLTSRYDELEKTSFDYAVVEHAE
SVFVHRYNGDWKDLGTWNTLTEEMTSTTHGQVDLFESTNTHVINELNLPLIGIGLEDLVVAVNADGILVSTKEATPQLKD
YLASSDDYPKFKWKRWGQSEVIHHETLADGHHSVTRRMLVQAGKELSYHYHEETDTIWTMLQGSATVTIDGETFEAKPTD
VISIQRGAAHTLKAKEDVVFIEVQYSNDWTLDDACPVPSPLDKQY

Specific function: Involved In The Biosynthesis Of The Capsular Polysaccharide Colanic Acid. [C]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=472, Percent_Identity=27.3305084745763, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 50026; Mature: 50026

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLLLSGGSGKRLWPLSNDINSKQFIRLLDDGHGSKQSMIERVFSQLSDMKLNQDAYIL
CEEEEEECCCCCEEECCCCCCCHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
TNAHQRDAIVYQIGTDANIITEPSRRDTFPAIVLGASYLNEQQVDPDETVLVMPVDAYVD
ECCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEEHHHHCCCCCCCCCEEEEEECCHHEE
LRFFEYFPQVDAAVQASDDALHLIGVTPLHPTSKYGYIVPDETAQVSSVRLFQEKPTVDV
EHHHHHCCCCCCEEECCCCCEEEEEECCCCCCCCCCEECCCCCCCHHEEEEECCCCCHHH
AEQLIGQGALWNCGIFACKLHLLLDELSERNLPTTYSDLTSRYDELEKTSFDYAVVEHAE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCC
SVFVHRYNGDWKDLGTWNTLTEEMTSTTHGQVDLFESTNTHVINELNLPLIGIGLEDLVV
EEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEECHHHEEE
AVNADGILVSTKEATPQLKDYLASSDDYPKFKWKRWGQSEVIHHETLADGHHSVTRRMLV
EEECCEEEEECCCCCHHHHHHHCCCCCCCCCHHHCCCCCCCEEHHHHCCCHHHHHHHHHH
QAGKELSYHYHEETDTIWTMLQGSATVTIDGETFEAKPTDVISIQRGAAHTLKAKEDVVF
HCCCCCCEEECCCCCCEEEEECCCEEEEECCCEECCCCCCEEEECCCCCCEEECCCCEEE
IEVQYSNDWTLDDACPVPSPLDKQY
EEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLLLLSGGSGKRLWPLSNDINSKQFIRLLDDGHGSKQSMIERVFSQLSDMKLNQDAYIL
CEEEEEECCCCCEEECCCCCCCHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
TNAHQRDAIVYQIGTDANIITEPSRRDTFPAIVLGASYLNEQQVDPDETVLVMPVDAYVD
ECCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEEHHHHCCCCCCCCCEEEEEECCHHEE
LRFFEYFPQVDAAVQASDDALHLIGVTPLHPTSKYGYIVPDETAQVSSVRLFQEKPTVDV
EHHHHHCCCCCCEEECCCCCEEEEEECCCCCCCCCCEECCCCCCCHHEEEEECCCCCHHH
AEQLIGQGALWNCGIFACKLHLLLDELSERNLPTTYSDLTSRYDELEKTSFDYAVVEHAE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCC
SVFVHRYNGDWKDLGTWNTLTEEMTSTTHGQVDLFESTNTHVINELNLPLIGIGLEDLVV
EEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEECHHHEEE
AVNADGILVSTKEATPQLKDYLASSDDYPKFKWKRWGQSEVIHHETLADGHHSVTRRMLV
EEECCEEEEECCCCCHHHHHHHCCCCCCCCCHHHCCCCCCCEEHHHHCCCHHHHHHHHHH
QAGKELSYHYHEETDTIWTMLQGSATVTIDGETFEAKPTDVISIQRGAAHTLKAKEDVVF
HCCCCCCEEECCCCCCEEEEECCCEEEEECCCEECCCCCCEEEECCCCCCEEECCCCEEE
IEVQYSNDWTLDDACPVPSPLDKQY
EEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1372980; 10952301 [H]